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Disinfectants & Stabilizers Mycoplasma Disinfection of all tissue culture equipment and work surfaces in the lab have to be performed regularly to prevent contaminations with mycoplasmas and other unwanted microorganisms, or to eliminate microbes once they have been detected. PromoKine offers different disinfectants ideal for laboratory surfaces and apparatuses including CO2 incubators and workbenches, as well as solutions for maintaining clean water baths and water trays of CO2 incubators. elimination in cell/tissue cultures cytotoxicity Easy handling Low BIOMYC-1 is based on the antibiotic tiamutin, which is produced by the fungus Pleurotus mutilus. BIOMYC-2 is based on the antibiotic minocycline, an improved derivative of tetracycline. When used in combination these two antibiotics eliminate mycoplasmas very efficiently. Following our recommended protocol, the mycoplasmas do not develop resistance to these antibiotics - a common occurrence with other antibiotic treatment methods. Mycoplasma-ExS Spray Spore-EX Spray & Wipes Disinfection of laboratory surfaces and apparatuses effective against mycoplasmas and other micro- bial contaminants commonly found in cell culture labs Non-corrosive and non-irritant Long-lasting and cost-effective Highly BIOMYC-3 Mycoplasma elimination in cell/tissue cultures cytotoxicity Easy handling Low BIOMYC-3 is based on the antibiotic ciprofloxacin, which inhibits the synthesis of DNA gyrase. Many mycoplasma species are sensitive to BIOMYC-3, including M. orale, M. hyorhinis, M. fermentans and M. arginini, and Acheloplasma laidlawii. These species are responsible for most mycoplasma contaminations in cell culture. At the concentrations recommended for use, no cytotoxic effects have been found, and the treatment can be performed within 12 days. Product Size Mycoplasma-ExS Spray is highly effective against a broad spectrum of unwanted microbes commonly found in biological labs (e.g. bacteria) which has been validated according standard EN tests. It not only immediately disinfects the surface, but also provides long-term antimicrobial effects. This new solution allows fast, simple and safe cleaning and disinfection of all laboratory surfaces and apparatuses – preventing mycoplasma and other bacterial contamination. Mycoplasma-ExS is a non-irritant and non-corrosive solution that is free of alcohols and harmful volatile organic compounds (VOCs) such as aldehydes. Catalog Number PromoCidal Spray & Wipes Mycoplasma-EX 3 treatments PK-CC91-4003 BIOMYC-1 10 ml 20 ml 100 ml PK-CC03-036-1D PK-CC03-036-1C PK-CC03-036-1B BIOMYC-2 10 ml 20 ml 100 ml PK-CC03-037-1D PK-CC03-037-1C PK-CC03-037-1B BIOMYC-3 10 ml 20 ml 100 ml PK-CC03-038-1D PK-CC03-038-1C PK-CC03-038-1B PromoCidal has been validated according to different standard EN tests and eliminates bacteria (including mycoplasmas), fungi and viruses reliably within 5 minutes. It is also effective in denaturing DNA and RNA. PromoCidal is very safe and simple to use. The formulation contains only a very low concentration of isopropanol and is free of aldehydes, chlorine, phenols, peroxides as well as nonylphenol ethoxylates (NPEs). It is non-corrosive and virtually non-irritant. efficient against bacteria, fungi, and viruses Fast and reliable disinfection of surfaces Thoroughly validated against a broad range of microbes Safe and easy to use Free of aldehydes, phenols, and peroxides Available as ready-to-use spray and wipes efficient against bacteria, fungi, viruses, and spores fast and reliable disinfection of surfaces Thoroughly validated against a broad range of microbes Safe and easy to use Free of aldehydes, phenols, and peroxides Available as ready-to-use spray and wipes The water in an incubator or in a water bath is a major source of cell culture contamination, particularly with respect to mycoplasmas. Aquaguard, a non-volatile water stabilizer/cleaner, is extremely effective at maintaining the water quality in CO2 incubators (tray) or ordinary water baths. In addition, it does not cause damage to the stainless steel of the incubator or any plastic interior, and it does not cause any skin irritation when used as recommended. Microbial Detection and Elimination Aquaguard-1 is intended for maintaining the water quality in incubators (water trays). The solution is non-volatile and extremely effective for the upkeep of the water quality. Highly Very Aquaguard-2 is intended for maintenance of water quality in ordinary water baths and is highly effective to prevent growth of unwanted microorganisms. Product Spore-EX Spray and Wipes are reliable, all-around solutions for the highly effective and fast surface disinfection of critical workspaces. Spore-EX has a scientifically tested bactericidal, virucidal, fungicidal, tuberculocidal and sporicidal efficacy (according to different standard EN tests) and eliminates spores, viruses, bacteria and fungi (including mycoplasmas, Clostridium difficile spores, Hepatitis B/C, H1N1, MRSA, TB and NDM-1) reliably within 1 minute. Size Catalog Number 500 ml 10 x 500 ml PK-CC91-5051 PK-CC91-5051-10 PromoCidal Spray 500 ml 5l PK-CC91-5052 PK-CC91-5052-5L PromoCidal Wipes Mycoplasma-ExS Spray 100 pieces PK-CC91-5052-W100 Spore-EX Spray 750 ml PK-CC91-5053 Spore-EX Wipes 120 pieces PK-CC91-5053-W120 Aquaguard-1 (incubator) 100 ml 10 x 100 ml 3 x 100 ml PK-CC01-867-1B PK-CC01-867-10B PK-CC01-867-3B Aquaguard-2 (water bath) 500 ml PK-CC91-916-500 Mycoplasma Disinfection Kit I 1 Kit PK-CC91-5061 Aquaguard (Consists of MycoplasmaExS Spray 500 ml, Aquagard-1, and Aquagard-2) Maintenance Mycoplasma Disinfection Kit II 1 Kit PK-CC91-5071 Spore-EX is very safe and simple in its use, non-corrosive, non-irritant and dermatologically tested. It is a unique formulation containing only a very low concentration of propanol and is free of aldehydes, chlorine, phenols, peroxides as well as nonylphenol ethoxylates (NPEs). Highly of water quality in CO2 incubators and water baths Effectively prevents growth of unwanted microbes Non-corrosive, safe and non-volatile (Consists of PromoCidal Spray 500 ml, Aquagard-1, and Aquagard-2) For prices please see www.promokine.info/pricelist/ 07/2016 BIOMYC-1 and 2 Your PromoCell brand for biomedical research. Mycoplasma and Bacteria Test Kits M and bacteria detection in cell cultures, cell/virus stocks, and medium Highly sensitive detection of all mycoplasma and eubac- teria species relevant for cell/tissue culture contamination Fast, reliable and reproducible results Convenient protocol and easy sample preparation 1 2 3 4 5 6 Mycoplasma 479 bp 270 bp Detection of mycoplasmas utilizing a conventional light microscope is nearly impossible. In addition, cloudiness of media, typical for stronger bacterial or yeast contaminations, does not occur. PCR on the other hand is a highly sensitive, specific, and rapid method for the detection of even very low mycoplasma or bacterial contamination levels in culture supernatant, cell/virus stocks, or medium. Detection of mycoplasmas with the PCR Mycoplasma Test Kit I/C. M: 100 bp DNA ladder 1: Positive control 2: 10 mycoplasmas/sample volume 3: 100 mycoplasmas/sample volume 4: 1,000 mycoplasmas/sample volume 5: 10,000 mycoplasmas/sample volume 6: 100,000 mycoplasmas/sample volume Internal control DNA: 479 bp band Positive sample/control: 270 bp band PCR Mycoplasma Tests Kits I & II PCR Mycoplasma Test Kit I/RT The PCR Mycoplasma Test Kit I/C utilizes conventional PCR technology for the convenient, specific and highly sensitive detection of up to 40 mycoplasma and acholeplasma species, including all species known to contaminate cell cultures. The test can be performed within 3 hours and includes Internal Control DNA (as negative control and for verifying a successful PCR run) as well as Positive Control DNA. All components come optimally premixed and lyophilized in PCR tubes. After reconstitution with Reaction Buffer, the sample is added to this master mix and the PCR can be started. The PCR Mycoplasma Test Kit II also includes all components required for PCR premixed at optimized concentrations - however, in a ready-to-use (liquid) master mix. It also includes Positive Control DNA but in contrast to the PCR Mycoplasma Test Kit I/C it does not contain an Internal Control DNA. The PCR Mycoplasma Test Kits I/RT has been developed for convenient, highly sensitive, fast and reproducible RealTime PCR detection of mycoplasma contamination. The kit is compatible with any Real-Time PCR instrument able to detect the fluorescence dyes FAM™ and HEX™, and includes Internal Control DNA (for verifying a successful PCR run) as well as Positive Control DNA. All components come optimally premixed and lyophilized in PCR tubes. After reconstitution with Reaction Buffer, the sample (and – for kit variants A and B – Hot-Start Taq polymerase) is added to this master mix and the PCR can be started. DNA standards to perform a quantitative PCR analysis are available separately. 2 µl supernatant Analyze 5 µl of the PCR sample Boil for 10 minutes and centrifuge briefly Mycoplasma detection with the PromoKine PCR Mycoplasma Test Kit I/C is easy and convenient. Mycoplasma Elimination In addition, PromoKine provides a highly sensitive and time-saving PCR Bacteria Test Kit for detection of contaminating bacteria in cell culture media and virus stocks. It is applicable for 45 bacteria species and detects as little as 12 bacterial genomes (~52 fg) per sample volume. The ready-to-use kit includes positive and internal controls and a convenient protocol for easy sample preparation. Mycoplasma contaminations seriously affect cell growth and cell physiology leading to biased and unreliable experimental results. Standard antibiotics commonly used in cell culture (e.g. Pen/Strep) do not affect mycoplasma growth. Therefore, PromoKine offers two highly effective methods to get rid of mycoplasma contamination: Mycoplasma-EX, a combination of non-antibiotic and antibiotic reagents, and BIOMYC, three highly effective antibiotics. Mycoplasma-EX Mycoplasma 200 - 500 µl cell culture supernatat Extract Bacterial DNA 5 µl DNA sample Analyze 5 µl of the PCR sample PCR Bacteria detection with the PromoKine PCR Bacteria Test Kit is easy and convenient. Product Size Catalog Number PCR Mycoplasma Test Kit I/C 24 tests 48 tests 96 tests PK-CA91-1024 PK-CA91-1048 PK-CA91-1096 PCR Mycoplasma Test Kit I/RT; Variant C 25 tests 50 tests PK-CA91-3025C PK-CA91-3050C PCR Mycoplasma Test Kit II 20 tests PK-CA20-700-200 PCR Bacteria Test Kit 24 tests 48 tests PK-CA91-2024 PK-CA91-2048 elimination in cell/tissue cultures and virus stocks Very short treatment time Very low cytotoxicity Easy handling PromoCell GmbH Sickingenstr. 63/65 69126 Heidelberg Germany USA/Canada Phone: 1 – 866 – 251 28 60 (toll free) Fax: 1 – 866 – 827 92 19 (toll free) Germany Telefon: 0800 – 776 66 23 (gebührenfrei) Mycoplasma-EX combines a non-antibiotic and a thoroughly adjusted antibiotic treatment. It eliminates mycoplasmas by directly killing them and not just by inhibiting their growth, while showing extremely low toxicity for the cells in culture. Mycoplasma-EX is effective with only one treatment, destroying mycoplasmas fast and permanently. It is applicable for most cell lines (e.g. Vero, BHK21, GBK, ML, Hep2, 293, CRFK, H9, Molt4, MT-4, Jurkat) as well as virus stocks (e.g. SHV-1, BHV-1, HSV-1, VSV, SFV, FCV, MEV) and highly effective against all mycoplasma species usually encountered as contaminants in cell cultures. Fax: 0800 – 100 83 06 (gebührenfrei) France Téléphone: 0800 – 90 93 32 (ligne verte) Téléfax: 0800 – 90 27 36 (ligne verte) United Kingdom Phone: 0800 – 96 03 33 (toll free) Fax: 0800 – 169 85 54 (toll free) Other Countries Phone: +49 6221 – 649 34 0 Fax: +49 6221 – 649 34 40 Email:[email protected] [email protected] PCR 100 µl cell culture supernatant Bacteria Test Kit When using the PCR Mycoplasma Test Kit II, the sample is directly added to the ready-to-use, complete reaction mix to set up the PCR. For prices please see www.promokine.info/pricelist/ © PromoCell GmbH www.promocell.com | www.promokine.info Fluorescence microscopic image of cells contaminated with mycoplasmas.