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Mitosis in Animal and Plant Cells Purpose: To observe mitosis in animal and plant tells and to determine the relative length of each phase. Materials: slide of onion root tip mitosis slide of whitefish blastula mitosis Word Meanings: Mitosis- cell division telo- end phase- part of cycle, stage kinesis- division meta- among, with, after microscopes ` inter- between cyto- cell pro- before, forward karyo- nucleus an a- equal quantity ana- equal quanity Materials: 1. Write the meaning for each word written in the results number one by using the prefixes and suffixes written in the methods. 2. Observe the prepared microscope slide of whitefish blastula undergoing mitosis, and for each phase listed in results number 2 draw in what you see. 3. Observe the prepared microscope slide of onion root tip undergoing mitosis and for each phase listed in results number 3 draw in what you see. 4. Select region of root tip you want to observe. Look at each cell and record what type of cell it is, do this in a systematic manner so you don't miss any cell or count the cell twice. Calculate total number of cells observed. Use this to calculate the percentage of cells in each phase. If a complete cycle of mitosis (including interphase) takes 960 minutes, use the % to determine the time for each phase to occur. (960 minutes times the percent) Results: 1. karyo kinesis________________ telo phase ____________________ meta phase ___________________ inter phase - ___________________ cyto kinesis - ______________ prophase - _______________ anaphase - _______________ 2. Whitefish blastula: interphase prophase metaphase anaphase telophase 3. Onion Root tip: interphase prophase metaphase anaphase telophase 4. PHASE NUMBER OF CELLS PERCENTAGE OF CELL TIME Interphase Prophase Metaphase Anaphase Telophase Total of all phases Conclusions: 1. List the different phases of mitosis in order. 2. Does mitosis occur in both animal and plant cells? How do you know? 3. The slides that we observed is a whole bunch of cells in the mitosis process. Each cell is undergoing mitosis as needed. When we look, (or preserve) the cells at one instant the cells are preserved in the stage that they were going through. If the majority of the cells are of one phase does this mean that this phase lasted the longest or shortest? 4. With number 3 in mind which phase lasted the longest? Discussion: 1. Why is mitosis so important for the organisms? 2. Which cells in the organisms undergo mitosis? 3. Which process occur first karyokinesis or cytokinesis? 4. How can you differentiate the appearance between prophase from metaphase? 5. What are the differences between animal and plant cell mitosis?