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Methylation Patterns in the LCR Region of HPV-16 and HPV-31 Cervical Samples Leticia Rojas Mentor: Mina Kalantari Cervical cancer is highly associated with infection of certain types of Human Papillomaviruses (HPV). Over 90% of cervical cancers have been found to progress from those HPV’s that are particularly “high-risk”. Included in these “high-risk” HPV’s are HPV-16 and HPV-31, which is what this project has worked on thus far. This lab researches the methylation patterns of different HPV type positive cervical samples. Previous studies have shown that methylation of HPV genomes, at cytosine residues, may suppress the progression of lesions and the development of cancer. The general goal is to sequence high-risk HPV genomes and to establish a consistent pattern of methylation and correlation with cancer development. We are investigating if there is a correlation between the methylation pattern of the HPV genome and cervical carcinogenesis. The project begins with the extraction of DNA from cervical tissue samples, which are then modified by bisulfite modification. After modification, the products are amplified by polymerase chain reaction, cloned and sequenced. Sequence analysis is performed to determine the methylation status of each CpG site.