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Project Based Biosafety Form Information about the sample sources and potentially infectious agents is critical for effective biosafety measures. Consequently, this sample information form must be filled out completely and signed by the Principal Investigator who requests samples to be sorted in the FICR Core. This form and Sort Request Form must be submitted for review for FICR Director before processing experiments or projects are scheduled in Researcher: (individual performing experiment) Phone: E-mail: Lab Location (Building/Room #): Principal Investigator : Phone: E-mail: List type of sample and source (i.e., mouse spleen cells, human peripheral blood mononuclear cells, cells from an animal en-grafted with human cells, etc.); for cell lines, describe from what type of cell the cell line was derived (e.g. murine B-lymphocyte.) Does the sample contain any known Infectious agent(s)? If Yes, list agent(s): YES NO Has the Infectious agent(s) been inactivated or rendered non-infectious? (circle one) YES NO Unknown If yes, describe the method of inactivation. Has this protocol been reviewed by the Institutional Biosafety Committee? (circle one): YES NO (If yes, state BSL and approval number and date of approval) Were tissue/blood donors screened for the following pathogens: HIV, SIV, HepB, HepC, HepD, Herpesvirus simiae, HTLV-1, HTLV-2, LCMV, SARS, Mycobacterium tuberculosis or Mycobacterium bovis or Neisseria meningitidis? (circle one): YES NO Results: (circle one): Unknown Positive Negative Could the sample contain other known human pathogens? If yes, list agent(s): Yes No Were cells transformed using a virus such as EBV, HTLV-1, herpes, Saimirii or other virus? If yes, please list virus: YES NO Were the cells genetically engineered? YES NO If yes, how were they genetically engineered? Was a viral vector (adenovirus, retrovirus, lentivirus, herpesvirus, etc.) used to transfer genetic information to the cells? If yes, is the used virus: (circle one) replication-competent. or replication-incompetent ? If yes describe method and show packaging cell line. Will the samples be fixed prior to submission to core flow cytometry laboratory? YES NO Please describe the fixation protocol in detail, e.g. list concentration and exposure time. I have read the above questions carefully and certify that the information provided to be accurate and correct. Signature: (Researcher):_______________________________Date:______________ Signature: (Principal Investigator)________________________Date:______________ Review: (Circle One) Approved Disapproved The FICR Core: Copy to be retained on file by Flow & Imaging Cytometry Resource.