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Reading Guide, Stryer Short Course, Chapter 31 1.What is nitrogen fixation? How much ATP does it cost to form two molecuels of ammonia one molecule of N2? 2. Plants and micororganisms can incorporate ammonia into amino acids by reductive amination of a-ketoglutarate to form __________________. 3. Ammonia can also be incorporated into glutamate to make _____________________ at the cost of one ATP. 4. List the nonessential amino acids for humans. 5. Which three amino acids can be made by a one-step transamination? What cofactor is necessary for this reaction? 6. What three steps are necessary to transform 3-phosphoglycerate into serine? 7. When glycine is formed from serine, the one carbon sidechain is transferred to the cofactor ________________________, which is an important one-carbon carrier. 8. Another important one-carbon carrier is the molecule ________________________. It is formed from reaction of methionine and ATP. 9. The production of SAM utilizing THF is termed the ________________________ cycle. 10. How does enzyme multiplicity function in regulation? What is cumulative feedback inhibition?