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Download Immunogenicity and Protective Efficacy of Lipopeptides
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Immunogenicity and Protective Efficacy of Lipopeptides Vaccine Containing Human Asymptomatic CD4 and CD8 T Cell Epitopes in Human Leukocyte Antigen (HLA) Transgenic Rabbits Arfan Azeem Mentor: Lbachir BenMohamed Evidence obtained from animal models and humans suggest that CD8+ T cells specific for HSV-1 contribute to protective immunity against herpes infection. The purpose of this study is to test and analyze the immunogenicity (induction in vivo and localization of the CD8+ T-cells) and protective efficacy of lipopeptides vaccine containing human asymptomatic CD4 and CD8 T cell epitopes on ocularly-infected human leukocyte antigen (HLA) transgenic rabbits. I had the responsibility to test one parameter of the vaccine immunogenicity using immunostaining to localize trigeminal ganglia (TGs) infiltrating CD8+ T-cells and one parameter of the protective efficacy using virus titration, which is a quantitative technique that measures the amount of herpes virus present in the rabbit’s eye during ocular herpes disease. We have shown that Lipopeptides immunizations induced strong CD8+ T-cells infiltration in HSV-1 infected TGs and strongly decreased the HSV replication over time. The lipopeptides vaccine strategy, containing human asymptomatic CD4 and CD8 T cell epitopes, induced in vivo strong T-cell responses that infiltrated the infected tissue (TGs) and reduced HSV replication in the eye during ocular herpes disease.
