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CATALOG OF ANTIBODIES FOR MARKERS Stem Cell • Neural • Fibroblast • Organelle • Cancer For research purposes only. Not for use in humans. Prices subject to change. FAX: 303.730.1966 • WEB: www.novusbio.com 2 Table of Contents Stem Cell Markers................ 2-5 Marker Antibodies Stem Cell Marker Antibodies Hematopoietic Stem Cell .............................. 2-5 Mesenchymal Stem Cell .................................... 2 Embryonic Stem Cell .................................... 2-5 Neuronal Stem Cell .......................................... 3 Neural Markers ..................6-12 Keratinocyte Stem Cell ...................................... 3 Hemangioblast Stem Cell .................................. 5 Fibroblast Marker Antibodies ..................... 6 Neural Cell Marker Antibodies Organelle Cell Markers .... 12-16 Musashi 1 Astrocyte/Astroglia Markers ............................... 6 General Glial Cell Markers................................ 7 Microglia Markers ............................................. 7 Neuronal Markers...................................... 8-10 Oligodendrocyte Markers .......................... 10-11 Cancer Markers C M k ............... 17-22 Schwann Cell Markers.................................... 11 Organelle Cell Marker Antibodies Autophagic Vesicles Markers........................... 12 Centrosome Markers...................................... 12 Endosome Marker.......................................... 13 GFAP Endoplasmic Reticulum Markers.............................. 13 Application Key Golgi Complex .............................................. 14 ELISA - Elisa Lysosome Markers ......................................... 15 FACS - Fluorescent Activated Cell Sorting Mitochondrial Markers ............................ 15-16 ICC - Immunocytochemistry Nuclear Markers ............................................ 16 IF - Immunofluorescence IHC - Immunohistochemistry IHC-Fr - Immunohistochemistry Frozen IHC-P - Immunohistochemistry Paraffin IP - Immunoprecipitation RIA - Radioimmunoassay Cancer Marker Antibodies Breast Cancer.......................................... 17-19 Colorectal Cancer .................................... 19-20 Lung Cancer ........................................... 20-21 Prostate Cancer .............................................. 22 Beta Tubulin 3 WB - Western Blot Reactivity Key Am - Amphibian Ha - Hamster Av - Avian Hu - Human Bb - Baboon Ma - Mammal Bv - Bovine Mk - Monkey Ca - Canine Mu - Mouse Ch - Chicken Po - Porcine Dr - Drosophila Rb - Rabbit Eq - Equine Rt - Rat Fe - Feline Ye - Yeast Gp - Guinea Pig Xp - Xenopus Gt - Goat 1 UCHL1 TOLL FREE: 888.506.6887 • PHONE: 303.730.1950 Daily product updates! www.novusbio.com Stem Cell Markers Functionally, stem cells are able to propagate and generate additional stem cells, and can differentiate into various progenitor cells which further maturate along specific lineages. Stem cell markers allow for the identification and isolation of stem cells in different types of tissues and cell populations. Such markers allow identification of embryonic, hematopoietic, mesenchymal/stromal, and neural stem cells. Embryonic stem (ES) cells, derived from the inner cell mass of preimplantation embryos, are recognized as CD34 Antibody Hematopoietic Stem Cell Marker NB600-1071 Staining of murine hair follicles using NB600-1071. Species: Mu Applications: FACS, IHC, IHC-P, IP, WB CD34 Antibody Hematopoietic Stem Cell Marker NB100-1934 Staining of human tonsil using NB100-1934. Species: Hu, Mk Applications: IHC, IP, WB the most pluripotent population, having the greatest developmental potential. However, for many applications adult stem cells may be preferred due to ethical issues involved in the derivation of ES cells. Stem cell research is poised to allow the development of new therapies through repair, replacement, and regeneration of damaged or diseased cells and tissues making it one of the most exciting frontiers in modern medicine. CD133 Antibody Hematopoietic Stem Cell Marker NB120-16518 CD117 Antibody Hematopoietic Stem Cell Marker NB600-765 CD133 is a cell-surface protein that identifies neural stem cells, which give rise to neurons and glial cells. This antibody is expressed on primitive hematopoietic stem and progenitor cells, retinoblastoma, hemangioblasts, and neural stem cells as well as on developing epithelia. CD117 is expressed on the surface of hematopoietic stem cells as well as other cell types. This receptor binds to stem cell factors. Species: Hu, Mu Applications: ELISA, ICC, IF, IHC, IHC-P, WB CD105 Antibody Mesenchymal Stem Cell Marker NB600-1011 This 90 kDa disulfide-linked homodimer is expressed by vascular endothelial cells. It is suggested that CD105 functions in adhesion and embryonic angiogenesis. Species: Mu Applications: FACS, IHC-Fr, IP, WB The highly glycosylated 75-120 kD antigen CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Staining for CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence. Species: Hu, Mk Applications: FACS ABCG2 Antibody Hematopoietic Stem Cell Marker NB100-2177 ABCG2 (ATP-binding cassette superfamily G member 2) is a hematopoietic stem cell marker that is found in a wide variety of stem cells. Species: Hu Applications: ICC, IHC-Fr, IHC-P, WB CRIPTO Antibody Embryonic Stem Cell Marker NB100-1597 Detection of Cripto in MDA-MB21 lysate using NB100-1597 CRIPTO Antibody Embryonic Stem Cell Marker NB100-1598 Detection of Cripto in MDA-MB21 lysate using NB100-1598. Species: Hu Applications: WB Species: Hu Applications: WB The cripto gene is expressed both in embryonic stem cells and during the early phases of embryo development, while, in the adult, it is reactivated in a wide range of epithelial cancers. Connexin 43 Antibody NB100-722 Detection of Connexin 43 in rat hippocampal lysate (showing specifity, lane 1) and lambda-phosphatase incubated rat Species: Hu, Mu, hippocampal lysate Mk, Rt (negative control, lane Applications: WB 2) using NB100-722. For research purposes only. Not for use in humans. Prices subject to change. Connexin 43 Antibody NB300-309 Detection of Connexin 43 in rat cerebellar lysate using NB300-309. Species: Hu, Mu, Mk, Rt Applications: WB Connexin 43 is a negative cell surface marker for the stem cell-containing population of human limbal epithelial cells. Connexin 43 forms gap-junctional channels and regulates the permeability of these gap junctions to small organic molecules. Gap junctional intercellular communication is thought to play a key role in development and may also be involved in epilepsy. FAX: 303.730.1966 • WEB: www.novusbio.com 4 Keratin 19 Antibody Pancreatic Stem Cell Marker NB110-56914 Staining of HeLa cells using NB110-56914. Musashi-1 Antibody Neuronal Stem Cell Marker NB100-1759 Staining of neural rosettes using NB100-1759. PSCA Antibody Prostate Stem Cell Marker NB120-15168 Staining of human breast cancer using NB120-15168. Species: Hu Applications: IHC, ICC, FACS, IP, WB Species: Hu, Mu, Rt Applications: ICC, IHC, IHC-P, WB Musashi-1 is important for cell fate determination, with a role in maintenance of the stem cell state, differentiation and tumorigenesis. Species: Hu Applications: IHC-P NB100-58842 NB100-588 CK19 is synthesized mainly in embryonic and adult simple epithelia, but has also been found in stratified epithelia as well. NANOG Antibodies Embryonic Stem Cell Markers Nanog is a molecular marker that recognizes the undifferentiated state of stem cells in mouse and human. It is a divergent homeodomain protein that directs propagation of undifferentiated ES cells. Nanog-deficient embryonic stem cells lose pluripotency and differentiate into extraembryonic endoderm lineage. Species: Mu Applications: IP, WB Species: Mu Applications: IHC NB100-56450 NB110-40414 NB100-59737 Detection of Nanog in HeLa nuclear lysates using NB100-59737. Species: Hu Applications: ELISA, WB Detection of Nanog in mouse embryonic stem cells using NB100-588. Staining of mouse teratoma using NB100-58846. Detection of Nanog in (A) HeLa and (B) NIH/3T3 cells using NB100-56450. Detection of Nanog in human hippocampus tissue lysate using NB110-40414. Species: Hu Applications: WB Species: Hu, Mu, Mk, Gt Applications: WB p63 Antibody Keratinocyte Stem Cell Marker NB110-57175 Staining of paraffinembedded human breast carcinoma using NB110-57175. Nestin Antibody Neuronal Stem Cell Marker NB300-265 Detection of nestin in human fetal temporal lobe tissue lysates using NB300-265. Lane 1: 0.5 ug/ml Lane 2: 2 ug/ml Nestin Antibody Neuronal Stem Cell Marker NB100-1604 Species: Hu, Mu, Rt Applications: FACS, IHC, WB Species: Hu Applications: WB Species: Species: i Mu Mu Applications: ICC, IHC p63 Antibody Keratinocyte Stem Cell Marker NB110-57309 Immunofluorescent staining of A431 cells using NB110-57309. Staining of neural stem cells of the ventricular zone of the CNS using NB100-1604. Nestin is a Class VI intermediate filament expressed in the developing CNS in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Podocalyxin Antibody Hematopoietic Stem Cell Marker NB110-41503 Detection of podocalyxin in human lung lysate using NB110-41503. Podocalyxin Antibody Hematopoietic Stem Cell Marker NB110-41510 Detection of podocalyxin in human lung lysate using NB110-41510. Species: Hu Applications: WB Species: Hu Applications: WB Species: Hu Applications: ICC, IP, WB The p63 gene is highly expressed in the basal or progenitor layers of many epithelial tissues. p63 plays a fundamental role associated with development and differentiation. 5 Podocalyxin is a CD34 family member expressed by podocytes, vascular endothelia, mesothelia, and a subset of hematopoietic progenitors. Podocalyxin appears to complement CD34 as a useful cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells. TOLL FREE: 888.506.6887 • PHONE: 303.730.1950 Daily product updates! www.novusbio.com Nucleostemin Antibody Embryonic Stem Cell Marker NB100-1569 Detection of human and mouse GNL3 in HeLa, 293T and 3T3 cells using NB100-1569. Nucleostemin Antibody Embryonic Stem Cell Marker NB100-2896 Staining of human skin basal cells carcinoma using NB100-2896. Nucleostemin Antibody Embryonic Stem Cell Marker NB100-1568 Detection of human and mouse GNL3 in HeLa, 293T and 3T3 cells using NB100-1568. Species: Hu, Mu Species: Hu, Mu Species: Hu, Mu Applications: IP, WB Applications: IHC Applications: IP, WB Nucleostemin is a protein found in the nucleoli of embryonic stem cells, adult CNS stem cells, primitive cells in the bone marrow, and cancer cells. It is not in the differentiated cells of most adult tissues. It has been suggested to play a role in controlling the cell-cycle progression in stem cells and cancer cells. The transcription factor OCT4, expressed in ES cells and germ cells, is strongly implicated in the process of maintaining as well as regaining stem-cell pluripotency and functions as a key regulator of mammalian germline development. It is expressed in totipotent embryonic stem and germ cells. A critical level of Oct-4 expression is required to sustain stem cell self-renewal and pluripotency. SSEA Antibodies Embryonic Stem Cell Markers SSEA-1 is expressed on the surface of preimplantation-stage murine embryos and has been found on the surface of teratocarcinoma stem cells, but not on their differentiated derivatives. SSEA-3 is synthesized during oogenesis and is present in the membranes of oocytes, zygotes and early cleavage-stage embryos. Biological roles of these carbohydrate-associated molecules have been suggested in controlling cell surface interactions during development. Undifferentiated primate ES cells, human EC and ES cells express SSEA-3, but not SSEA-1. Undifferentiated mouse ES cells express SSEA-1, but not SSEA-3. SSEA-1 staining has been seen in the the oviduct epithelium, endometrium, epididymis, and in some areas of the brain and kidney tubules in adult mice. SSEA1 Antibody NB100-1831 Species: Hu, Mu Applications: FACS, ICC, IF SSEA3 Antibody NB100-1832 Species: Hu Applications: FACS, IF, IHC For research purposes only. Not for use in humans. Prices subject to change. OCT4 Antibody Embryonic Stem Cell Marker NB100-2379 Detection of OCT4 in HeLa whole cell extract using NB 100-2379. OCT4 Antibody Embryonic Stem Cell Marker NB100-41088 Detection of OCT4 in human liver lysate using NB100-41088. Species: Hu Applications: WB Species: Hu Applications: ELISA, WB Sca-2 Antibody Hematopoietic Stem Cell Marker NB100-58797 Staining of cerebellar Purkinje neurons using NB100-58797. Sox2 Antibody Embryonic Stem Cell Marker NB110-37235 Detection of SOX2 in mouse brain lysate using NB110-37235. Species: Hu Applications: IHC-P, IP, WB Species: Mu Applications: IHC, WB The autosomal dominant cerebellar ataxias (ADCA) are a heterogeneous group of neurodegenerative disorders characterized by progressive degeneration of the cerebellum, brain stem and spinal cord. The SOX (SRY-box containing gene) gene family encodes a group of transcription factors defined by the conserved high motility group (HMG) DNA-binding domain. They are involved in the regulation of embryonic development and in the determination of cell fate. Telomerase Antibodies Embryonic Stem Cell Markers NB100-317 Telomeres are nucleoprotein structures at the ends of eukaryotic chromosomes. Telomerase is an enzyme uniquely associated with immortal cell lines and is useful for identifying undifferentiated PSCs. Staining of normal pancreas using NB100-317. Species: Hu Applications: FACS, IF, WB NB100-297 NB120-32020 Detection of hTERT in MJ90 whole cell lysate using NB100-297. Staining of paraffinembedded human lung adenocarcinoma using NB120-32020. Species: Hu Applications: FACS, ICC, IHC, WB Species: Hu Applications: IF, WB FAX: 303.730.1966 • WEB: www.novusbio.com 6 Thrombopoietin Antibody Hematopoietic Stem Cell Marker NB110-68129 Detection of TPO Lane 1: 100ng mouse TPO (non-reduced) Lane 2: 100ng mouse TPO (reduced). Species: Mu Applications: ELISA, WB TRA 1-60 Antibody Embryonic Stem Cell Marker NB100-730 TRA-1-81 and TRA-1-60 are cellsurface proteins involved in cell differentiation. Cell surface antigens are powerful tools for characterizing the function and expression of developmentally regulated cells during both embryogenesis and oncogenesis. Thrombopoietin (TPO) has been shown to function both as a proliferative and differentiative factor for megakaryocytes and as a survival and weakly proliferative factor for hematopoietic stem cells. TRA 1-81 Antibody Embryonic Stem Cell Marker NB100-1833 Immunofluorescence staining of methanol-fixed NTERA-2 cl.D1 cells showing membrane localization using NB100-1833. Species: Hu Applications: FACS, IF, IHC, IP, WB Detection of TRA1-60 in NTERA-2 cell lysate using NB100-730. Species: Hu S Applications: FACS, IF, IHC, IP, WB A VEGFR2 Antibodies Hemangioblast/Stem Cell Markers Receptors for Vascular Endothelial Growth Factor (VEGF), VEGFB and PGF have a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. VEGF and its high-affinity binding receptors, the tyrosine kinases FLK1 and FLT1, are thought to be important for the development of embryonic vasculature. It has been shown that an alternately spliced form of FLT1 produces a soluble protein, termed sFLT1, which binds vascular endothelial growth factor with high affinity, playing an inhibitory role in angiogenesis. NB200-208 NB100-686 Staining of FFPE human angiosarcoma using NB100-686. Species: Hu, Mu, Rt Applications: IF, IHC-Fr, IHC-P, WB Species: Hu, Rt Applications: ELISA, FACS, IHC NB100-527 NB100-627 Species: Hu Applications: WB Species: Hu, Mu Applications: IP, WB NB110-55547 NB100-530 7 NB100-529 Detection of VEGFR-2 in CSF-1 receptor/ VEGFR2 chimera transfected lysate using NB100-529. Species: Hu Applications: WB NB110-55548 Staining of breast carcinoma using NB110-55548. Species: Hu Applications: IHC Species: Hu Applications: WB NB110-57644 NB110-57149 Detection of VEGFR2 doublet in CSF-IR/VEGFR2 chimera transfected lysate using NB100-2382. Species: Hu Applications: WB Species: Hu, Mu, Rt Applications: IHC-P, WB Detection of VEGFR2 in VEGFR2 induced HUVEC lysate using NB100-530. Staining of breast carcinoma using NB110-55547. NB100-2382 Staining of formalin fixed paraffin embedded human angiosarcoma using NB600-1433. Detection of VEGFR2 in CSF-1/VEGFR2 transfected lysates using NB100-627. Lane 1 - 1:2,000 Lane 2 - 1:10,000 Detection of VEGFR2 in Chimeric CSF-1R/VEGFR2 using NB100-527. Species: Hu Applications: IHC NB600-1433 Staining of myocardium section using NB200-208. The arrowheads indicate VEGFR-2 positive endothelial cells from myocardial capillaries. Detection of VEGFR2 in ECV304 cell lysate using NB110-57644. Detection of VEGFR2 in ECV304 cell lysate using NB110-57149. Species: Hu Applications: WB Species: Hu Applications: WB TOLL FREE: 888.506.6887 • PHONE: 303.730.1950 Daily product updates! www.novusbio.com Fibroblast Markers Fibroblasts (Pan Reticular) Antibody BM4018 Staining of reticular fibroblasts and reticular fibres with BM4018 antibody. Fibroblast (Surface Protein) Antibody SM1214P Staining of KG1 cells with mouse anti human fibroblast antibody (SM1214P). Prolyl 4-Hydroxylase Antibody AF5110 Staining of frozen rat skin sections using AF5110. Species: Hu, Mu Applications: IHC-Fr Species: Hu Species i H Applications: FACS, IHC, IP Species: Rt Applications: ELISA, IHC, WB Neural Cell Markers Astrocyte/Astroglia Markers Astrocytes are star-shaped glial cells that perform multiple functions in the CNS. They play a significant role in maintaining normal brain physiology during development and in adulthood. Astrocytes also provide alpha Smooth Muscle Actin Antibody NB120-18460 physical support to neurons, clean up debris within the brain, provide chemicals to help control fluid composition surrounding neurons, and provide nutritional support to neurons. Survivin Antibody NB500-201 ABCA1 Antibody NB400-105 Telophase with accumulation of survivin in the midbodies of two daughter cells. Staining of human leiomyosarcoma using NB120-18460. Staining of prostate epithelium using NB400-105. Species: Bv, Ch, Hu, Ma, Mu, Rt, Bb Applications: IF, IHC-Fr, IHC-P, WB Species: Hu, Mu, Ca, Rt, Fe Applications: IF, WB, IHC-P, IP, ICC. Species Species: Species i Ha, Ha H a Hu, Hu Mu, H Mu Rt M Applications: FACS, ICC, IF, IHC-P, IP, WB Smooth muscle vessels alpha actin has been found in distinct populations of astrocytes in the adult mouse brain, in particular in the corpus callosum and the fornix. However, in the corpus callosum, it is seen only in parasagittal planes. Thus, alpha smooth muscle actin represents a new marker for subsets of astrocytes. Survivin is also abundantly expressed in brain tissues (astrocytes and some neurons) of adult rats following traumatic brain injury. Survivin has been found co-expressed with NeuN (mature neuronal marker) and PCNA (a cell cycle protein). Survivin might affect regulation of neural cell proliferative responses after brain injury. In the brain, ABCA1 is expressed in neurons, astrocytes, and microglia and is induced by liver X receptor (LXR) and retinoid X receptor (RXR) agonists. GFAP Antibody NB300-141 Staining of typical astrocytic cells using NB 300-141 in neurons and glia of rat forebrain. Species: Bv, Hu, Mu, Po, Rt, Fe Applications: ICC, IF, IHC, IHC-Fr, IHC-P, WB GFAP Antibody NB300-142 Rat cortical neurons and glia in mixed tissue culture stained with Chicken polyclonal to MAP2 NB300-213 (green) at 1/30000 and Mouse Species: Hu, Ma monoclonal to GFAP Applications: IF, WB - NB300-142 (red) at 1/100. GLAST Antibody NB100-1869 GLAST expression begins in the newborn and persists throughout life. This consistency and the fact that GLAST expression is linked to a functional role of astrocytes in glutamate homeostasis makes it a good marker for astrocyte identification. Species: Rt Applications: FACS, ICC, IHC, WB Glial fibrillary acidic protein (GFAP) is a member of the class III intermediate filament protein family. It is heavily and specifically expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In addition neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells. In addition many types of brain tumors, presumably derived from astrocytic cells, heavily express GFAP. For research purposes only. Not for use in humans. Prices subject to change. FAX: 303.730.1966 • WEB: www.novusbio.com 8 General Glial Cell Markers Glial cells are non-neuronal cells that include microglia and macroglia cells. The latter includes astrocytes, oligodendrocytes, ependymal cells, radial glia, Schwann cells and satellite cells. Glial cells, which are often referred to as the glue of the CNS, function to support and protect neurons, maintain homeostasis, form myelin, and partake in signaling in the central nervous system (CNS). The SOX (SRY-box containing gene) gene family encodes a group of transcription factors defined by the Detection of SOX2 in conserved high motility group (HMG) mouse brain lysate DNA-binding domain. They are using NB110-37235 . involved in the regulation of embryonic development and in the determination of cell fate, and are expressed in the Bergmann glia, a Species: Mu population of radial glia present in Applications: IHC, WB the Purkinje cell layer. Sox2 Antibody NB110-37235 Sox2 Antibody NB110-37235 Staining of human uterus using NB110-37235. Species: Mu Applications: IHC, WB Vimentin Antibody NB110-57645 Staining of paraffinembedded human melanoma using NB110-57645. Vimentin (phospho Ser38) Antibody NB110-57646 Detection of Vimentin in 1) untreated and 2) Calyculin A treated HeLa cell lysate using NB110-57646. Vimentin (Phospho Ser72) Antibody NB110-57647 Detection of Vimentin in 1) untreated and 2) Calyculin A treated HeLa cell lysate using NB110-57647. Species: Hu, Mu, Rt Applications: IHC, IP, WB Species: Hu, Mu, Rt Applications: WB Species: Hu, Mu, Rt Applications: IP, WB Vimentin (Phospho Ser82) Antibody NB110-57648 Detection of Vimentin in 1) AP treated and 2) untreated HeLa + Calyculin A cell lysate using NB110-57648. Vimentin Antibody NB200-622 Staining of formalin-fixed, paraffin-embedded human osteosarcoma using NB200-622. S Species: i Hu, H Mu, M Rt R Applications: WB Species: All Applications: IHC-P, IP, WB Vimentin Antibody NB300-223 Rat glial cells in mixed cerebral cortex cultures stained with antivimentin (NB 300-223) [green] and counterstained with anti-GFAP (NB300-142) [red]. Species: Hu, Mu, Rt Hoechst dye reveals Applications: IF nuclear DNA [blue]. Vimentin is the 10nm or intermediate filament protein subunit found in many kinds of mesenchymal and epithelia cells, in many kinds of cells in tissue culture, and in developing neuronal and astrocytic precursor cells in the central nervous system. Vimentin frequently forms co-polymers with other intermediate filament proteins, such as GFAP (in astrocytes), with desmin (in muscle cells) and neurofilament proteins (in developing neurons). Antibodies to vimentin are useful in studies of stem cells and are generally used to reveal the filamentous cytoskeleton. Microglia Markers Microglia are the smallest glial cells that function similarly to macrophages in the blood system. They are the first immune defense in the CNS that seek and destroy damaged neurons, plaques, and infectious agents. When infections cross the blood-brain barrier, microglia cells act to increase inflammation in order to destroy the infectious agents before they cause damage to the brain and/or spinal cord. Due to their plasticity, microglia cells are present in various forms depending on their location in the body, such as ameboid, ramified, phagocytic, juxtavascular, and gitter cells. 9 CD11 b/c Antibody Ferritin Antibody NB110-40766 NB600-920 Staining of bone marrow, myeloid precursors using NB110-40766. Detection of Ferritin in HT1080 cells using NB600-920 using IP. Species: Hu, Mu Applications: IHC, IHC-P, WB Species: Hu Applications: ELISA, IHC, IP, WB CD11b is expressed on 8% spleen cells, 44% bone marrow cells and less than 1% of thymocytes and is commonly used as a microglial marker in nervous tissue. Ferritin is a a marker for microglia, especially for reactive microglia and macrophages. TOLL FREE: 888.506.6887 • PHONE: 303.730.1950 Daily product updates! www.novusbio.com Neuronal Markers Neurons, the functional unit of the nervous system, are electrically excitable cells that transmit signals throughout the body. Generally composed of a soma, dendritic tree, and axon, their size, shape, and function varies greatly. Acetylcholinesterase Antibody Early Neuronal Development NB100-1519 Detection of ACHE in A431 lysate using NB100-1519. Bag 1 Antibody Neuronal Differentiation NB110-55489 Staining of paraffinembedded colon carcinoma using NB110-55489. Beta Tubulin 3 Antibody Dendrite Marker NB100-1612 Dissociated cell culture of a neonatal mouse brain showing beta Tubulin 3 (green staining) of neurons. Species: Hu, Mu Applications: ELISA, WB Species: Hu, Rt Applications: ICC, IHC Species: Mu Applications: ICC, IHC Acetylcholinesterase, or AChE, hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission Bag1 is a multifunctional protein that blocks apoptosis and interacts with several types of proteins, including the Bcl 2 family proteins. Beta III tubulin is abundant in the central and peripheral nervous systems (CNS and PNS) where it is prominently expressed during fetal and postnatal development. Bcl-2 Antibody Bcl-2 Antibody Neuronal Apoptosis Inhibitor Neuronal Apoptosis Inhibitor NB100-2087 NB200-572 Staining of Detection of Bcl-2 human tonsil using in human lung cell NB200-572. Note extract using absence of staining NB100-2087. of reactive cells in the germinal center. Species: Hu, Mk Applications: IHC-P, WB Species: Hu Applications: IHC Bcl-2 Antibody Neuronal Apoptosis Inhibitor NB300-900 Detection of Bcl-2 in human lymph node lysate using NB300-900. Bcl-2 Antibody Neuronal Apoptosis Inhibitor NB110-55551 Staining of paraffinembedded human breast carcinoma using NB110-55551. Species: Hu, Mu, Rt Applications: ELISA, WB Species: Hu, Mu Applications: FACS, ICC, IHC, IP, WB This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells. The broad range of protective effects of BCL-2 protein suggests that BCL-2 may interact with a central step in neural cell death. c-Fos Antibody Neuronal Activation NB600-904 Staining of rat spinal cord uisng NB600-904. Species: Hu, Mu, Rt Applications: IHC-P, IP, WB c-Fos Antibody NB120-14285 Detection of c-Fos in western blot. Species: Bv, Hu, Mu, Rt Applications: WB The Fos proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. Fos was the first transcription factor identified with a critical function in regulating the development of skeletal cells. Calretinin Antibody NB110-55651 Staining of paraffinembedded Human mesothelioma using NB110-55651. Calretinin Antibody NB300-707 Detection of Calretinin rat brain homogenates using NB300-707. Calretinin Antibody NB200-618 Staining of human mesothelioma using NB200-618. Note cytoplasmic and nuclear staining of tumor cells. Species: Hu, Mu, Rt Applications: IHC Species: Hu, Mu, Rt Applications: IP, WB Species: Hu, Mu, Rt, Am Applications: IHC-P Mesothelial Cell Marker: Calretinin (CAB29, calbindin 2) is a calcium binding protein that belongs to calbindin family. It is mainly found in neuronal tissue and is highly expressed in auditory neurons. It has been implicated in a calcium buffer, calcium sensor, and as a regulator of apoptosis. Calretinin is considered a specific marker of mesothelial cells; it is the only marker that has the utility in distinguishing between sarcomatoid mesotheliomas and sarcomatoid renal cell carcinomas. It is widely used in the panel of makers for distinguishing mesothlioma from adenocarcinoma. For research purposes only. Not for use in humans. Prices subject to change. FAX: 303.730.1966 • WEB: www.novusbio.com 10 ChAT Antibody Cholinergic Neuron Marker NB100-40794 Detection of ChAT in mouse brain lysate using NB100-40794. ChAT Antibody Cholinergic Neuron Marker R-043-100 Staining of rat facial nucleus using Rabbit antibody to ChAT (R-043-100) at 1:2000 dilution. S Species: i Rt, Rt Mu M Applications: ELISA, WB Species: Hu, Rt, Rb, Gp Applications: ELISA, IHC, WB ChAT serves as a specific marker for cholinergic neurons in both peripheral and central nervous systems. It is also present at parasympathetic postganglionic fibres. ELF Antibody Neuronal Precursor Cell Marker NB100-68253 Detection of Human ELF1 in HeLa whole cell lysates using NB100-68253. ELF Antibody Neuronal Precursor Cell Marker NB100-40828 Detection of SPTBN1 in HeLa and 293T cells using NB100-40828. ELF Antibody Neuronal Precursor Cell Marker NB100-55272 Staining of human prostate adenocarcinoma using NB100-55272. Species: Hu Applications: WB Species: Hu, Mu Applications: WB, IP Species: Hu Applications: IHC-P Spectrins play a pivotal role in axonal transport, neurite extension, the organization of synaptic vesicles, as well as for protein sorting in the Golgi apparatus and cell membrane. HSV-1 Antibody Neuronal Latency Marker NB110-57251 10-well HSV-1 slides, 10-well HSV-2 slides, anti-MouseFITC F(ab)2 with counterstain. Following primary infection in humans, herpes simplex virus type 1 (HSV-1) establishes latent infections in sensory neurons. Species: All Applications: ELISA, IF, WB MAP2 Antibody Neuronal Dendrite Marker NB300-213 Rat neurons stained with anti-MAP2 (NB300-213) [green] and anti-GFAP (NB300-142) [red]. Species: Bv, Hu, Mu, Rt Applications: IF, WB Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5. Antibodies to MAP2 are excellent markers on neuronal cells, their perikarya and neuronal dendrites. NQO1 Antibody Neuronal Injury Marker NB200-209 Detection of NQO1 in A549 lysates using NB200-209. Lane 1: 0.5 ug/ml. Lane 2: 2 ug/ml. Nestin Antibody Neuronal Stem Cell Marker NB300-266 Visualization of nestin in PC-3 cells using NB300-266. Courtesy of Dolnick Lab, Roswell Park Cancer Institute. Species: Ca, Hu, Mk, Rt Applications: IF, IHC, IP, WB Species: Hu Applications: ICC, IHC, IF, WB Neuronal damage is associated with an increased activity of NQO1. Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. Neurofilament Light Antibody NB300-133 Detection of NF-L in whole rat cerebellum homogenate using NB300-133. Neurofilament Medium Antibody NB300-134 Culture of adult neural cells. Mature neurons can be identified by morphology and staining. Neurofilament Heavy Antibody NB300-135 Section of rat spinal cord stained with NB300-135 (green) and NB110-58869 (red). Species: Bv, Hu, Ma, Mu, Po, Rt, Fe, Av Applications: ICC, IF, IHC-Fr, IHC-P, WB Species: Hu, Ma, Mu, Av Applications: IHC, WB Species: Hu, Ma, Mu, Mk Applications: IHC-Fr, IHC-P, WB Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-L runs on SDS-PAGE gels at about 68kDa; NF-M at 145-170 kDa; NF-H at 180-220 kDa. Antibodies to the neurofilaments are useful for identifying neuronal cells and their processes in tissue sections, and can also be useful in the diagnostics of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig’s disease or Alzheimer’s disease. 11 TOLL FREE: 888.506.6887 • PHONE: 303.730.1950 Daily product updates! www.novusbio.com PGP9.5/UCHL1 Antibodies Peripheral Nerve Fiber Markers PGP9.5 Antibody NB300-675 UCLH1 Antibody NB110-58872 PGP9.5 Antibody NB600-1160 Staining of human brain using NB300-675. Staining of rat abdominal tissue using NB600-1160. Species: Mu, Hu Applications: IHC, WB Species: Hu, Rt Applications: ELISA, IHC-P, WB PGP9.5 Antibody NB110-58874 S Shows rat mixed n neuron/glial cultures sstained with Rabbit UCHL1 (green). Blue U iis a DNA stain. Shows rat mixed neuron/glial cultures stained with chicken UCHL1 (green) and rabbit antibody to glial fibrillary acidic protein (GFAP-red), Species: Hu, Rt NB300-141. Blue is Applications: IF, WB a DNA stain. Ubiquitin C-terminal hydrolase 1 (UCHL1) was originally identified as a major component of the neuronal cytoplasm from 2-dimensional gel analysis of brain tissues, and was given the name PGP9.5. The protein is extremely abundant representing 1-2% of total brain protein. It was later found that a ubiquitin C-terminal hydrolase enzyme activity was associated with the PGP9.5 protein, resulting in the renaming of PGP9.5 to ubiquitin C-terminal hydrolase 1. Since UCHL1 is heavily expressed in neurons, antibodies to UCHL1 can be used to identify neurons in histological sections and in tissue culture. The great abundance of this protein in neurons means that it is released from neurons in large amounts following injury or degeneration, so the detection of UCHL1 in CSF and other bodily fluids can be used as a biomarker. UCHL1 was also discovered as a gene mutated in some rare famial forms of Parkinson’s disease. Interestingly, a common allelic variant of UCHL1, the S18Y polymorphism is actually protective against Parkinson’s disease. Species: Species: i Hu, Hu Rt Applications: IF, WB Synapsin 1 Antibody NB300-312 Detection of synapsin 1 in rat hippocampal lysate using NB300-312. Synapsin 1 Antibody NB300-104 Species: Hu, Mu, Rt Applications: WB Species: Hu, Mu, Rt Applications: IF, IHC, IP, WB Detection of synapsin 1 in rat hippocampal lysate using NB300-104. Synapsin I plays a key role in synaptic plasticity in the brain. This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. In addition to its role in plasticity, the expression of synapsin I is a precise indicator of synapse formation. Thus Synapsin I immunocytochemistry provides a valuable tool for the study of synaptogenesis. The role of synapsin in synaptic plasticity and in synaptogensis is regulated by phosphorylation. Oligodendrocyte Markers Oligodendrocytes provide support to axons and produce insulating myelin sheaths. The CNS depends on this insulation to reduce ion leakage in order to increase overall nerve conduction speed. When myelin Amphoterin Antibody NB100-2322 sheaths are degenerated oligodendrocytes attempt to replace them, however, in multiple sclerosis patients oligodendrocytes themselves are destroyed thus compromising their ability to perform repairs. CNPase Antibody NB100-1935 CNPase Antibody NB110-79874 Detection of CNPase in normal brain tissue lysate using NB110-79874. Detection of HMGB1 in whole cell HeLa lysate using NB 100-2322. Adult mouse brain showing CNPase (brown staining) in white matter tracts and the granule cell layer of the cerebellum. Species: Hu, Mu Applications: WB Species: Species: i Hu Hu Applications: WB The onset of amphoterin expression, prior to the expression of myelin-specific antigens, makes amphoterin a useful marker for studying oligodendrocyte development. 2’,3’-cyclic nucleotide 3’-phosphodiesterase (CNPase) is associated with brain tubulin and acts as a microtubule-associated protein in promoting microtubule assembly and distribution. CNPase is used as a marker of cell differentiation in the CNS as it is expressed primarily in committed oligodendroglial progenitors and terminally-differentiated oligodendrocytes. For research purposes only. Not for use in humans. Prices subject to change. Species: Mu Applications: ICC, IHC FAX: 303.730.1966 • WEB: www.novusbio.com 12 MAP-4 Antibody NB100-74615 Olig2 Antibody NB110-41440 MOG Antibody NB300-948 D Detection of human MAP4 in HeLa h ccell lysate using NB100-74615. u Detection of Olig 2 in Jurkat whole cell lysates using NB110-41440. Detection of MOG in human brain lysate using NB300-948. Species: Species: i Hu Hu Applications: IP, WB Species: Hu, Mu, Rt Applications: ELISA, WB Species: Hu, Mu Applications: WB Microtubule-associated protein 4 is a major non-neuronal microtubuleassociated protein and is a marker for astroglia and oligodendroglia cells. Myelin-Oligodendrocyte Glycoprotein (MOG) is a surface marker of oligodendrocyte maturation. The oligodendroglial lineage marker, Olig2, is expressed in neural progenitors and oligodendroglia and is essential for oligodendrocyte development. Nestin Antibody NB600-876 Nestin Antibody NB100-1604 Detection of Nestin in MEF whole cell lysates using NB600-876. A tissue section of mouse brain showing Nestin (green staining) in neural stem cells of the ventricular zone of the CNS. Species: Mu Applications: ICC, IHC Species: Mu Applications: WB Nestin is a marker of oligodendrocyte lineage cells. Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. Schwann Cell Markers Schwann cells are a type of glial cell that function as support cells for the PNS, similar to oligodendrocytes of the CNS. They mainly provide myelin insulation to AN2 Antibody NB300-750 peripheral axons in order to increase signal conduction. Schwann cells also aid in clearing debris and guide axon regrowth in the PNS. AN2 Antibody NB100-61654 Detection of AN2 in mouse eye lysate using NB100-61654. Detection of PAX6 in rat whole eye extract using NB 300-750. Species: Mu, Hu, Ch, Xp, Rt, Sh Applications: WB Species: Species: i Mu Mu Applications: ELISA, WB AN2 is expressed by immature Schwann cells in vitro and in vivo and downregulated as the cells upregulate myelin genes. Ran-1 Antibody NB100-1384 Species: Hu, Mu, Rt Applications: IHC-Fr, IHC-P, WB Laminin Antibody NB300-144 Detection of laminin in EHS (1) and heart (2) lysates using NB300-144. SCIP Antibody NB100-1359 D Detection of Ran-1 iin 3T3 cell lysate usiing NB100-1384. 13 Laminin Antibody NB300-144 Staining of rat spinal cord and dorsal root tissue using NB 300-144. Pepsin antigen retrieval was performed. Detection of SCIP in human brain lysate using NB100-1359. Species: Species: i Hu, Hu Mu Mu Applications: ELISA, WB Species: Hu Applications: WB Ran-1 is a marker for normal Schwann cells and is associated with adult non-myelin-forming Schwann cells. SCIP, also known as Tst-1 or Oct-6, is expressed in proliferating Schwann cells in the PNS and O2A progenitor cells in the developing CNS. Species: Hu, Mu, Rt Applications: IHC-Fr, IHC-P, WB Laminin is a marker for human Schwann cells in vitro. Laminin antibodies are widely used to label blood vessels and basement membranes. TOLL FREE: 888.506.6887 • PHONE: 303.730.1950 Daily product updates! www.novusbio.com Organelle Markers Autophagic Vesicle Markers Autophagy is an evolutionarily conserved process responsible for the bulk degradation of intracellular material. In autophagy, cytoplasmic components are engulfed by double-membrane-bound structures APG8a Antibody R-146-100 (autophagosomes) and delivered to lysosomes/vacuoles for degradation. Recent reports indicate that autophagy plays a crucial role in many different pathologies. APG8b Antibody R-155-100 Confocal microscopy on immunofluorescently detected MAP1LC3 A in cytospin-isolated human white blood cells. APG8c Antibody R-140-100 Confocal microscopy on immunofluorescently detected MAP1LC3 C in cytospin-isolated human white blood cells. Detection of APG8b in Olfactory cell line (Odora) lysate using R-155-100. Species: Hu, Rt Applications: IF, IHC, WB Species: Hu, Rt Applications: IF, IHC, WB Species: Hu Applications: IF, IHC, WB LC3 Antibody NB100-2220 LC3 Antibody NB100-2331 LC3B Antibody NB600-1384 Staining of brain, cerebral cortex, cell processes in gray matter using NB100-2331 Staining of brain, cerebral cortex, neurons using NB100-2220. Species: Hu, Mu, Rt Applications: IHC, IHC-P, IP, WB Species: Hu, Mu Applications: IHC, IHC-P, IP, WB Staining of treated U373-MG (human glioblastoma) cells using NB600-1384. Species: Hu, Mu Applications: ICC, IF, IHC, WB LC3 is the only known mammalian protein identified that stably associates with the autophagosome membranes. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. The detection of LC3-I to LC3-II conversion is a useful and sensitive marker for distinguishing autophagy in mammalian cells. Centrosome Markers The centrosome is the main microtubule organizing center (MTOC) of the cell and acts as a regulator of cell-cycle progression. Centrosomes are present in higher eukaryotic cells, but not plant cells. The centrosome is composed of two centrioles surrounded by an amorphous mass of pericentriolar material gamma Tubulin Antibody NB100-1628 Detection of gamma Tubulin in chicken fibroblasts using NB100-1628. Species: Bv, Ca, Ch, Ha, Hu, Mu, Rt, Xp Applications: ELISA, IHC, WB Gamma tubulin is responsible for binding microtubule minus ends and mediating the link between microtubles and the centrosome. For research purposes only. Not for use in humans. Prices subject to change. (PCM). The PCM contains proteins responsible for microtubule nucleation and anchoring. Each centriole is composed of nine triplet microtubule blades in a pinwheel structure. Cancer cells often have an abnormal number of centrosomes, which may be of biological significance. Pericentrin/Kendrin Antibody NB100-61071 Detection of Human Pericentrin/ Kendrin in HeLa cell extracts using NB100-61071. Pericentrin/Kendrin Antibody NB100-61072 Detection of Human Pericentrin/ Kendrin in HeLa cell extracts using NB100-61072. Species: Hu Applications: IP Species: Hu Applications: IP, WB Pericentrin binds to calmodulin and is expressed in the centrosome. It is an integral component of the pericentriolar material (PCM). The protein contains a series of coiled-coil domains and a highly conserved PCM targeting motif called the PACT domain near its C-terminus. The protein interacts with gamma-tubulin and is likely important to normal functioning of the centrosomes, cytoskeleton, and cell-cycle progression. Pericentrin/Kendrin Antibody NB100-68277 Staining of HeLa cells using NB100-68277. Species: Hu Applications: ICC FAX: 303.730.1966 • WEB: www.novusbio.com 14 Endosome Marker EEA1 Antibody NB300-502 Endosomes are membrane-bound vesicles that are formed by endocytosis. Endocytosis involves invagination of the plasma membrane to encircle a substance and budding to release the vesicle. Endosomes function to either transport their contents to a lysosome for degradation or to transport substances between different portions of the external cell membrane, the latter of which is essential for epithelial cells whose polarity differs on each side of the membrane. Staining of HeLa cells using NB300-502. Species: Ca, Ha, Hu, Mu, Rt, Xp Applications: IF, IP, WB Early endosomal antigen 1 (EEA1) is a 162 kDa membrane bound protein component specific to the early endosome and is essential for fusion between early endocytic vesicles. Endoplasmic Reticulum Markers The endoplasmic reticulum is an organelle of interconnected tubules, vesicles, and cisternae. Its specific functions involve protein translation and folding, WFS1 Antibody NB100-1918 Immunoprecipitation of WFS1 using NB 100-1918. Precipitated lysate is INS-1. Species: Hu, Mu, M Rt Applications: IF, IHC-Fr, IP, WB WFS1 plays an important role in maintaining homeostasis of the endoplasmic reticulum (ER) in the pancreas. transport of proteins to the cell membrane, secretion of cellular proteins, sequestration of calcium, and production and storage of macromolecules. PDI Antibody NB100-1921 Protein disulfide isomerase (PDI) is an acidic 55 kDa thyroid hormone-binding protein that appears to be identical to the glialp55 protein. It has been suggested to be involved in disulfide-bond formation and isomerization, as well as the reduction of disulfide bonds in proteins. ERp29 Antibody NB300-523 Species: Rt Applications: ICC, IP, WB ERp29 resides in the lumen of the ER and is thought to play a role in the processing of secretory proteins within the ER. Calreticulin Antibody NB600-103 Detection of calreticulin in human kidney lysate using NB 600-103. Calreticulin Antibody NB600-101 Immunofluorescence staining of Calreticulin in HCT15 colon cancer cells using NB 600-101. Species: Hu Applications: WB Species: Bv, Ha, Hu, Mu, Rt Applications: ICC, IF, IHC, IP, WB Calreticulin is a multifunctional, highly conserved Ca2+ -binding protein that is localized to the endoplasmic reticulum. GRP78/BiP Antibody NB300-520 Detection of GRP78 in liver, J77, CHQ and Akt-29 cells using NB300-520. 15 Staining of rat thyrocytes using NB 300-523. Species: Bv, Ca, Gp, Ha, Hu, Mu, Mk, Rt Applications: IF, IP, WB ERp57 Antibody NB100-1143 Detection of ERp57 in HepG2 lysate using NB100-1143. Species: Hu Applications: ELISA, WB ERp57 functions together with calnexin and calreticulin as a molecular chaperone of glycoprotein biosynthesis within the ER. Heme oxygenase-1 Antibody NB110-57028 GRP94 Antibody NB100-41095 Immunofluorescent staining of HeLa cells using NB110-57028. Detection of GRP94 in 3T3 lysate using NB100-41095. Species: Ha, Rt, Mu Applications: IF, IP, WB S i M Species: Mu Applications: ELISA, WB Within the ER, misfolded proteins are detected by Bip(GPR78). Glucose-regulated protein 94, also known as Grp94 or gp96, is an abundant resident endoplasmic reticulum (ER) lumenal stress protein. Species: Hu, Mu Applications: ICC, IHC, IP, WB HO-1 catalyzes the NADPH, O2 and cytochrome P450 reductase dependent oxidation of heme to carbon monoxide, iron and biliverdin that is immediately reduced to bilirubin. TOLL FREE: 888.506.6887 • PHONE: 303.730.1950 Daily product updates! www.novusbio.com Golgi Complex Markers The Golgi apparatus is a membrane-bound stack of cisternae that processes proteins and lipids synthesized in the ER and packages them for transport elsewhere in the cell. The Golgi is responsible for glycosylating proteins synthesized in the ER and also for cleaving some proteins into smaller fragments. The Golgi packages these products in vesicles for transport, either to destinations inside of the cell or outside of it. GlcNAc transferase Antibody NB100-57845 Golgi Marker 58K Antibody NB600-412 Glucose-6-Phosphate Antibody NB100-236 Detection of G6PD in HUVEC cells using NB100-236.. Detection of GlcNAc in rat pancreas lysate using NB100-57845. Staining of HeLa cells using NB600-412. Species: Ca, Ha, Hu, Rt Applications: ICC, IF, IHC, WB Species: Rt, Mu Applications: ELISA, WB Species: Hu, Mu Applications: IP, WB 58K protein antibodies are excellent for use as markers for the Golgi complex. GlcNAc transferase is present as an integral Golgi membrane protein and at least the catalytic center of the transferase is on the lumenal side of the vesicles. Glucose-6-Phosphate is an indigenous component of Golgi membranes. P58 Antibody NB110-57306 Syntaxin-6 Antibody NB100-2920 Detection of Syntaxin-6 in human lymph node lysate using NB100-2920. Detection of p58 in HeLa cell lysate using NB110-57306. S Species: i Hu H Applications: WB Species: Hu Applications: ELISA, WB P58 is a marker of pre-Golgi intermediate compartment (IC) and a putative marker for peripheral punctate pre-Golgi intermediates and the cis-Golgi network. Syntaxin-6 is primarily on the trans-Golgi network (TGN), where it partially colocalizes with the TGN adapter protein AP-1 on clathrin-coated membranes. VPS45 Antibody NB100-2431 TGN38 Antibody NB110-40768 Species: Mu, Rt Applications: IF, WB TGN38 Antibody NB110-60519 Immuno fluorescence staining of TGN38 in 3% paraformaldehydefixed NRK cells. VPS45 Antibody NB100-41372 Detection of VPS45 in human peripheral blood mononucleocyte lysate using NB100-41372. Detection of VPS45 in human placenta lysate using NB100-2431. Species: Hu Applications: ELISA, WB Species: Hu Applications: WB VPS45 is a member of the sec1 family implicated in Golgi to prevacuolar compartment trafficking in yeast. Mammalian VPS45 is found in multiple tissues, is partially membrane associated, and is enriched in the Golgi region. TGN46 Antibody NB110-62093 Species: Hu, Mk Applications: IF, WB Species: Mu, Rt Applications: IF, IHC-P, WB TGN38 is a a 38 kDa protein which has been shown to be predominantly localized to the trans Golgi network. TGN46 Antibody NB110-60520 TGN46 Antibody NB110-40769 Immunofluorescence staining of human TGN46 in 3% paraformaldehydefixed HeLa cells. Immunofluorescence staining of human TGN46 in 3% paraformaldehydefixed HeLa cells. Detection of TGN46 in HeLa cell lysate using NB110-40769. Species: Hu, Mk Applications: IF, WB Immunofluorescence staining of TGN38 in 3% paraformaldehyde-fixed NRK cells. Species: Hu, Mk Applications: IF, IHC-P, WB TGN46 is localized primarily in the Trans-Golgi network. TGN46 has been reported as being the best available marker for human trans-Golgi network. For research purposes only. Not for use in humans. Prices subject to change. FAX: 303.730.1966 • WEB: www.novusbio.com 16 Lysosome Markers Lysosomes are membrane-bound vacuoles which play a critical role in cellular metabolism. These organelles participate in endocytosis, the synthesis and assembly of LAMP1 Antibody NB120-19294 acid hydrolases, act as sites for digestion of foreign materials and for specialized autolytic cellular processes. LAMP2 Antibody NB110-11470 Staining of mouse astrocytes using NB110-11470. Immunofluorescence staining of Lamp1 (red) in HeLa cells using N120-19294. LIMPII/lgp85 Antibody NB400-129 Staining of LIMP II in rat infarctzone tissue using NB 400-129 with a DAB detection system. Species: Hu Applications: IF Species: Ha, Hu, Mu, Mk, Rt, Rb Applications: ICC, IF, IHC, IHC-P, IP, WB Species: Ca, Hu, Mu, Rt Applications: ICC, IHC, IP, WB Lamp1, also known as lysosome associated membrane protein 1, is a heavily glycosylated membrane protein of the lysosome. Lysosome-associated membrane protein 2 (LAMP 2), also known as lgp2 or lgp110, is a type 1 integral membrane protein that is transported from the trans-Golgi network to endosomes and then lysosomes. LIMP II is ubiquitously expressed in most higher organisms and is a major lysosomal and endosomal membrane marker. Rap1 Antibody NB100-292 Rap1 Antibody NB110-57453 Localization of RAP1 to Telomeres with TRF2 by Immunofluorescence, using NB 100-292. Sample: HeLa cells. Species: Hu Applications: IF, IP, WB Rap1 Antibody NB110-57454 Staining of paraffinembedded human stomach carcinoma using NB110-57453. Species: Hu, Mu, Rt Applications: FACS, ICC, IHC, IP, WB Staining of paraffinembedded human breast cancer tissue using NB110-57454. Species: Hu, Mu, Rt Applications: FACS, ICC, IHC, IP, WB Rap1A and Rap1B proteins were localized to late endocytic compartments (late endosomes/lysosomes) in fibroblasts. Mitochondrial Markers The mitochondrion is a compartmentalized, dualmembraned organelle that is responsible for most energy production in the cell. Mitochondria contain their own DNA and ribosomes. The primary function of mitochondria is to convert energy from glucose into ATP via oxidative phosphorylation via the Krebs Cycle. Cytochrome c Antibody NB100-56503 Detection of Cytochrome C in HeLa cell lysate using NB100-56503. Cytochrome c Antibody NB300-1068 Staining of heart tissue using NB300-1068. Species: Dr, Ha, Eq, Hu, Mu, Rt Applications: ICC, IF, WB Species: Hu Applications: ELISA, IHC-P, WB GFP Antibody NB100-1770 GFP Antibody NB600-597 GFP Antibody NB600-303 Recombinant GFP detected using NB600-303. Species: All Applications: ICC, IHC-P, IP, WB Cytochrome c is released from mitochondria after tissue injury. It is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in the process of oxidative phosphorylation and production of cellular ATP. GFP recombinant protein detected with NB 100-1770 polyclonal anti-GFP antibody. Western blot of GFP recombinant protein detected with NB600-597. Species: All Applications: ELISA, IHC, WB Species: All Applications: ELISA, WB GFP, green fluorescent protein, is a marker for subcellular localization and as a label for plant mitochondria in vivo. 17 TOLL FREE: 888.506.6887 • PHONE: 303.730.1950 Daily product updates! www.novusbio.com Glutamate dehydrogenase Antibody NB600-853 HSP60 Antibody NB110-57063 Staining of paraffinembedded human human breast carcinoma using NB110-57063. Detection of Glutamate dehydrogenase in bovine liver preparations using NB600-853. HSP70 Mitochondrial Antibody NB300-527 Staining of human peripheral blood mononucleocyte lysate using NB300-527. Species: Bv Applications: ELISA, WB Species: Hu, Mu, Rt Applications: IHC, WB Species: Hu, Mu Applications: ICC, IP, WB Glutamate dehydrogenase has long been used as a marker for mitochondria in brain and other tissues. HSP60 is a transiently expressed mitochondrial protein. HSP70 is known to have been acquired from the proto-mitochondrial endosymbiont. GRK2 Antibody NB100-2831 GRK2 Antibody NB300-156 Detection of GRK2 in Daudi cell lysate using NB100-2831. NB300-156 (green) versus NF-M (red, NB 300-134), in rat cortex, showing mostly large pyramidal neurons. Species: Hu, Bv, Rt Applications: IHC, WB Species: Hu Applications: ELISA, WB GRK2 is found in many cell types and may also have a role in the regulation of the microtubular cytoskeleton. The levels of GRK2 enzymatic activity, protein and mRNA are all elevated in a variety of human heart disease states, and the effect of this may contribute to the reduced effectiveness of the beta-adrenergic system in these disease states. GRK2 is also overexpressed in Alzheimer disease and is an early marker of brain mitochondrial lesions. Mitochondrial Marker Antibody NB120-17857 Staining of human liver using NB120-17857. VDAC1 Antibody NB100-695 Species: Ca, Hu, Mu, Rt, Fe Applications: IF, IP, WB Species: Hu Applications: IHC-P Species: Hu, Rt Applications: ELISA, WB Analysis of the human heart mitochondrial proteome shows that mitofilin is one of the most abundant mitochondrial proteins. NB120-17857 recognizes a 60kDa non-glycosylated protein component of mitochondria found in human cells. It can be used to stain the mitochondria in human samples as a mitochondrial-marker in subcellular fractions. VDAC1 is an outer membrane mitochondrial protein. VDACs are highly expressed in heart, liver and skeletal muscle, where concentrations of mitochondria are at their highest. Mitofilin Antibody NB100-1919 Staining of HeLa cells using NB100-1919. . Nuclear Markers The nucleus is the central organelle of the cell, containing a cell’s DNA and coordinating the actions of the cell and the other organelles within the cell. Surrounded by the nuclear envelope, the nucleus is responsible for the control of cell division and the NUP214 Antibody NB110-40634 direction of protein synthesis within the cell. The nucleolous, a membraneless organelle lying within the nucleus, manufactures ribosomes for protein synthesis in the cell. Histone H1 Antibody NB300-373 Staining of human ovarian carcinoma using NB110-40634. Detection of VDAC1 in rat heart lysate using NB100-695. Detection of Histone H1 in calf thumus using NB300-373. Fibrillarin Antibody NB300-269 Human SH-SY5Y cells stained with NB300-269, showing prominent specular nucleolar stainingNB. Species: Hu Applications: ICC, IHC, IHC-P Species: Bv, Ch, Hu, Mu, Rb, Rt Applications: IHC, WB Species: Hu Applications: WB Nucleoporin 214 (NUP214) is required for CRM1-mediated nuclear protein export as well as cell cycle progression. Somatic H1 may act as a marker of nuclear reprogramming. Fibrillarin is an snRNP protein involved in pre-ribosomal RNA processing. For research purposes only. Not for use in humans. Prices subject to change. FAX: 303.730.1966 • WEB: www.novusbio.com 18 Cancer Markers Breast Cancer Breast cancer is the most common cancer in women and is second to lung cancer as the most common non-skin cancer overall. Approximately 200,000 cases of breast cancer are diagnosed in women every year in Androgen Receptor Antibody NB120-9474 the United States alone, with an additional 2,000 cases diagnosed in men. There will be an estimated 40,000 women and 450 men who die in 2008 from breast cancer in the United States. Androgen Receptor Antibody NB300-615 Bcl-2 Antibody NB200-572 Staining of formalinfixed, paraffinembedded human tonsil using NB200-572. Staining of primate brain using NB300-615. Staining of formalin fixed paraffin embedded human prostate using NB120-9474. Species: Hu Applications: IHC, WB Species: Hu, Mu, Mk, Rt Applications: ICC, IHC, IP, WB Species: Hu, Mk Applications: IHC-P, WB Beta-Catenin Antibody NB110-56924 CAIX Antibody NB100-417 CaR Antibody NB300-601 Staining of Rat-1 cells using NB300-601. Staining of human RCC tumor cryosections using NB100-417. Immunofluorescent staining of A431 cells using NB110-56924. Species: Hu, Mu Applications: ICC, IHC, IP, WB Species: Ca, Hu Applications: IF, IHC-P, WB Species: Hu, Rt Applications: ICC, IHC, IP, WB CD24 Antibody NB110-500 Cyclin A Antibody NB110-56899 Cyclin A Antibody NB110-14736 Staining of formalin fixed paraffin embedded human tonsil using NB110-14736. Staining of paraffinembedded skin carcinoma using NB110-56899. Staining of formalinfixed, paraffinembedded human liver using NB110-500. Species: Hu Applications: IHC Species: Hu Applications: ICC, IHC, IP, WB Species: Hu Applications: IHC, WB Cyclin A Antibody NB110-56898 Cyclin E Antibody NB110-56904 Cyclin E Antibody NB110-56905 Immunofluorescent staining of HeLa cells using NB110-56898. Staining of paraffinembedded human breast carcinoma using NB110-56905. Immunofluorescent staining of HeLa cells using NB110-56904. Species: Hu Applications: ICC, IP, WB Species: Hu Applications: FACS, ICC, IP, WB Species: Hu Applications: ICC, IHC, IP, WB DAP-kinase Antibody NB110-56925 DAP-kinase Antibody NB110-56926 ErbB2 Antibody NB100-1739 Staining of HeLa cells using NB110-56925. Species: Hu Applications: ICC, IHC, IP, WB 19 Staining of human breast adenocarcinoma using NB100-1739. Immunohistochemical staining of paraffinembedded human liver carcinoma using NB110-56926. Species: Hu, Mu, Rt Applications: FACS, IHC, WB Species: Hu Applications: IHC TOLL FREE: 888.506.6887 • PHONE: 303.730.1950 Daily product updates! www.novusbio.com ErbB-2 Antibody NB110-55529 ErbB-2 Antibody NB110-57022 ErbB-2 Antibody NB110-57021 Immunohistochemical staining of paraffinembedded Human breast carcinoma using NB110-57021. Staining of breast carcinoma using NB110-55529. Immunohistochemical analysis of paraffinembedded human breast carcinoma using NB110-57022. Species: Hu Applications: FACS, IHC, IP, WB Species: Hu Applications: IHC Species: Hu Applications: IHC ErbB-2 Antibody NB110-57023 EZH2 Antibody NB110-56975 GATA3 Antibody NB600-1184 IHC staining of paraffin embedded human tonsil using NB600-1184. Immunofluorescent staining of HeLa cells using NB110-56975. Immunohistochemical staining of paraffinembedded human breast ductal carcinoma in situ using NB110-57023. Species: Hu Applications: FACS, ICC, IHC, IP Species: Hu, Mu, Rt Applications: FACS, ICC, IF, IP, WB Species: Hu Applications: IHC, WB GCDFP-15 Antibody NB110-66658 Glut-1 Antibody NB120-15310 HIF-1 alpha Antibody NB100-479 Staining of placenta, villi using NB100-479. Staining of human esophagous using NB120-15310. Immunohistochemical staining of paraffinembedded human breast carcinoma using NB110-66658. Species: Hu Applications: FACS, ICC, IHC, WB Species: Hu, Rt Applications: IHC-P Species: Hu, Mu, Mk, Rt Applications: IHC, WB HSP70 Antibody NB110-57064 Ki67 Antibody NB500-170 KPNA2 Antibody NB100-1371 Staining of green formaldahyde-fixed HeLa with endogenously expressed Histone H2B-GFP fusion as DNA marker. Staining of FFPE human tonsil using NB500-170. Immunohistochemical staining of paraffinembedded human prostate carcinoma using NB110-57064. Species: Hu Applications: FACS, IHC, IP, WB Species: Hu, Mu, Po, Rt Applications: ICC, IF, IHC-Fr, IHC-P Species: Hu Applications: ELISA, ICC, IHC, WB Laminin Antibody NB300-144 MDM2 Antibody NB100-62269 MMP-1 Antibody NB110-57211 Immunohistochemical staining of paraffinembedded human cervical carcinoma using NB100-57211. Staining of MDM2human breast carcinoma using NB100-62269. Staining of rat spinal cord and dorsal root paraformaldehyde/ paraffin-embedded tissue using NB300-144. Species: Hu, Mu, Rt Applications: IHC-Fr, IHC-P, WB Species: Hu Applications: IHC-Fr, IHC-P, IP, RIA, WB Species: Hu Applications: FACS, ICC, IHC, IP, WB MMP-13 Antibody NB110-60992 MMP-2 Antibody NB200-193 p53 Antibody NB100-1746 Staining of formalin fixed and Paraffin embedded human placenta tissue using NB110-60992. Species: Hu Applications: IHC, WB For research purposes only. Not for use in humans. Prices subject to change. Staining of human skin squamous cell carcinoma using NB100-1746. Detection of MMP2 in a paraffin-embedded human neurofibroma using NB200-193. Species: Ch, Hu, Mu, Rt Applications: ELISA, IHC-Fr, IHC-P, WB Species: Hu Applications: IHC FAX: 303.730.1966 • WEB: www.novusbio.com 20 PKC alpha Antibody NB110-57353 p63 Antibody NB100-691 PSA Antibody NB600-835 Staining of formalin-fixed, paraffin-embedded human prostate carcinoma using NB600-835. Staining of human thyroid gland carcinoma using NB110-57353. Staining of formalin fixed paraffin embedded human skin using NB100-691. Species: Hu, Mu, Rt Applications: IF, IHC-P, IP, WB Species: Hu, Mu, Rt Applications: FACS, ICC, IHC, IP, WB Specie Species: cies i s: Hu Hu Applications: IHC-P, WB SNCG Antibody NB110-60497 Survivin Antibody NB500-238 Survivin Antibody NB500-201 Species: Hu Applications: ELISA, IHC-Fr, IHC-P, WB Telophase with accumulation of survivin in the midbodies of two daughter cells. Staining of ovary carcinoma using NB500-238. Immunohistochemical staining of paraffin-embedded human ovary carcinoma using NB110-60497. Species: Hu, Mu, Rt Applications: ICC, IF, IHC, IHC-P, WB Species: Hu, Mu, Ca, Rt, Fe Applications: IF, WB, IHC-P, IP, ICC. Colorectal Cancer Although only a quarter of diagnosed colorectal tumors are malignant, this portion still accounts for 150,000 cases of colorectal cancer in the United states each year, resulting in nearly 50,000 deaths. Nearly all Bcl-2 Antibody NB200-572 Caspase 7 Antibody NB110-55664 CDX-2 Antibody NB110-40734 Staining of FFPE human ovarian carcinoma using NB110-40734. Immunofluorescent staining of HeLa cells using NB110-55664. Formalin-fixed, paraffin-embedded human tonsil stained using NB 200-572. Species: Hu, Mk Applications: IHC-P, WB Species: Hu Applications: ICC, IHC, IP, WB Species: Hu Applications: IHC CDX-2 Antibody NB100-699 Cyclooxygenase-2 Antibody NB100-689 Galectin-3 Antibody NB300-538 Immunohistochemical staining using NB100-699. Staining of formalin fixed paraffin embedded human colon carcinoma using NB100-689. Staining of rat olfactory bulb using NB300-538. Species: Hu Applications: IHC Species: Hu, Mu, Rt Applications: IHC-Fr, IHC-P, IP, WB S Species: i Hu, H Mu, M Rt Applications: ELISA, FACS, IF, IHC, IP, WB Ki67 Antibody NB500-170 Ki67 Antibody NB600-1209 MMP-9 Antibody NB110-57222 Staining of FFPE human tonsil using NB500-170. Species: Hu, Mu, Po, Rt Applications: ICC, IF, IHC-Fr, IHC-P 21 malignant colorectal tumors are adenocarcinomas, although lymphomas, leiomyosarcomas, and carcinoids can also occur in the colorectal region. Staining of human lymph node using NB600-1209. Species: Hu Applications: IHC, IHC-Fr, WB Immunohistochemical staining of paraffinembedded human lymphoma using NB110-57222. Species: Hu Applications: FACS, ICC, IHC, WB TOLL FREE: 888.506.6887 • PHONE: 303.730.1950 Daily product updates! www.novusbio.com MMP-9 Antibody NB100-59029 MMP-9 Antibody NB110-57223 Immunohistochemical staining of paraffinembedded human breast carcinoma using NB110-57223. MUC1 Antibody NB120-15481 Staining of skin granulation tissue using NB100-59029. Staining of breast carcinoma using NB120-15481. Species: Hu Applications: FACS, IHC, WB Species: Hu Applications: IHC-P Species: Hu, Mu Applications: IHC-P, WB Osteopontin Antibody NB600-1043 p63 Antibody NB110-57309 PGP9.5 Antibody NB600-1160 Staining of human breast tumor cells using NB600-1043. Immunofluorescent staining of A431 cells using NB110-57309. Staining of rat abdominal tissue using NB600-1160. Species: Ca, Hu, Po Applications: IHC, WB Species: Hu Applications: ICC, IP, WB Species: Hu, Rt Applications: ELISA, IHC-P, WB SMAD4 Antibody NB110-57582 STAT3 Antibody NB110-57596 Species: Hu, Mu, Rt Applications: FACS, ICC, IHC, IP, WB S Species: i Hu H Applications: ICC, IHC, IP, WB Telomerase Antibody NB600-476 Immunofluorescence microscopy of Saos-2 cells transduced with a retroviral vector expressing hTERT and GFP rom an internal ribosomal entry site. Species: Hu Applications: IF, IHC, IP, WB Telomerase Antibody NB100-317 TEM-8 Antibody NB110-17405 VEGF-D Antibody NB110-60973 Immunohistochemical staining of paraffinembedded human lung carcinoma using NB110-57582. Staining of normal pancreas using NB100-317. Species: Hu Applications: FACS, IF, WB Immunohistochemical staining of paraffinembedded lung squamous carcinoma using NB110-57596. Immunohistochemical staining of human brain tissue using NB110-17405. Species: Hu, Mu, Rt Applications: IHC, WB Formalin fixed and Paraffin embedded human placenta tissue using NB110-60973. Species: Hu Applications: IHC, WB Lung Cancer Lung cancer is the most common non-skin cancer, with an estimated 215,000 new cases diagnosed in 2008 in the United States. Additionally, more than 160,000 people will die in the United States from lung cancer in 2008, making lung cancer the leading cause of cancer MUC16 Antibody NB200-562 Staining of human lung tissue (alveolocytes type II) using NB200-562. Species: Hu Applications: ELISA, IHC-P, WB For research purposes only. Not for use in humans. Prices subject to change. deaths. While most cases of lung cancer are related to smoking, there are other risk factors including genetics, family history of lung cancer and exposure to chemicals and pollution. Calcitonin Antibody NB110-55646 Immunohistochemical staining of paraffinembedded human thyroid using NB110-55646. CEA Antibody NB120-15987 Species: Hu, Mu, Rt Applications: IHC Species: Hu Applications: ELISA, IHC-P Staining of formalin fixed paraffin embedded human colon carcinoma using NB120-15987. FAX: 303.730.1966 • WEB: www.novusbio.com hTERT Antibody NB100-317 CEA Antibody NB120-15287 COX-2 Antibody NB100-689 Staining of pancreatic carcinoma using NB100-317. Staining of formalin fixed paraffin embedded human breast using NB120-15287. Staining of formalin fixed paraffin embedded human colon carcinoma using NB100-689. Species: Hu, Mu, Rt Applications: IHC-Fr, IHC-P, IP, WB S Species: i Hu H Applications: IHC Species: Hu Applications: FACS, IHC-P, IF, WB Ki67 Antibody NB110-57147 Ki67 Antibody NB600-1252 MAP-2 Antibody NB110-58369 MAP-2-positive cultured neurons (green) from an e13.5 mouse brain. Nuclei are blue. Staining of formalinfixed, paraffinembedded human tonsil using NB600-1252. Immunohistochemical staining of paraffinembedded Human tonsil using NB110-57147. Species: Hu, Mu, Rt Applications: IHC Species: Hu, Rt Applications:IHC-Fr, IHC-P Species: Mu Applications: ICC, IHC, IHC-P, WB MAP-2 Antibody NB300-213 NSE Antibody NB110-58870 NSE Antibody NB100-1606 Rat neurons stained with anti-MAP2 (NB 300-213) [green] and anti-GFAP (NB 300-142) [red]. Dissociated cell cultures of neonatal mouse brains, showing NSE-2 (green staining) in neurons. Staining of HEL293 cells using NB110-58870. Species: Bv, Hu, Mu, Rt Applications: IF, WB Species: Hu, Rt Applications: IF, WB Species: Mu Applications: ICC, IHC Osteopontin Antibody NB600-1043 Staining of human breast tumor using NB600-1043. Osteopontin Antibody NB100-1883 Staining of paraffin embedded human tonsil using NB100-1883. p63 Antibody NB100-691 Species: Ca, Hu, Po Applications: IHC, WB Species: Hu Applications: ELISA, IHC, IHC-P, WB Species: Hu, Mu, Rt Applications: IF, IHC-P, IP, WB p63 Antibody NB110-57309 PGP9.5 Antibody NB300-675 Smac Antibody NB110-56936 Staining of A431 cells using NB110-57309. Staining of formalin fixed paraffin embedded human skin using NB100-691. Immunohistochemical staining of paraffinembedded human skin cancer tissue using NB110-56936. Staining of human brain using NB300-675. Species: Hu Applications: ICC, IP, WB Species: Mu Applications: IHC, WB Species: Hu, Rt Applications: FACS, ICC, IHC, IP, WB Survivin Antibody NB500-237 Telomerase Antibody NB600-476 UCHL1 Antibody NB300-676 Staining of lung carcinoma using NB500-237. Species: Hu Applications: IF, IHC, WB Staining of human brain using NB300-676. Immunofluorescence staining of Saos-2 cells transduced with a retroviral vector expressing hTERT using NB600-476. S Species: i Hu H Applications: IF, IHC, IP, WB Species: Mu Applications: IHC, WB TOLL FREE: 888.506.6887 • PHONE: 303.730.1950 Daily product updates! www.novusbio.com Prostate Cancer For men in the United States, prostate cancer is the most common non-skin cancer and is the second most deadly cancer. With over $8 billion spent annually in the United States on prostate cancer research, prostate cancer diagnoses and deaths have declined in recent years after rising in the 1980s because of new detection techniques. Nearly 1 in 6 men will be diagnosed with prostate cancer at some point in their lives. Caveolin-1 Antibody NB100-615 Bcl-2 Antibody NB110-55551 Staining of EaHy926 endothelial cell line using NB100-615. Immunohistochemical staining of paraffinembedded human breast carcinoma using NB110-55551. Caveolin-1 Antibody NB110-39015 Staining of respiratory epithelium and alveole using NB110-39015. Species: Hu, Mu Applications: FACS, ICC, IHC, IP, WB Species: Hu, Mu, Rt Applications: IF, IP, WB Species: Hu Applications: IHC Caveolin-1 Antibody NB110-39016 DPP4 Antibody NB100-58756 DPP4 Antibody NB100-59021 Staining of respiratory epithelium, bronchial smooth muscle, and alveoli using NB11039016. Staining of placenta using NB100-58756. Staining of placenta using NB100-59021. Species: Hu Applications: IHC Species: Hu Applications: IHC-P Species: Hu Applications: IHC-P E-cadherin Antibody NB110-56937 Immunohistochemical staining of paraffinembedded human hepatocellular carcinoma using NB11056937. HGF Antibody NB110-57200 Ki67 Antibody NB600-1209 Species: Hu, Mu Applications: ICC, IHC, IP, WB Species: Hu Applications: ICC, IHC, WB Species: Hu Applications: IHC, IHC-Fr, WB LDH Antibody NB110-57161 MCM7 Antibody NB110-57188 PCNA Antibody NB500-106 Immunohistochemical staining of paraffinembedded human breast carcinoma using NB110-57200. Immunohistochemical staining of paraffinembedded human testis carcinoma using NB110-57161. Staining of human lymph node using NB600-1209. Immunohistochemical staining of paraffinembedded human lung tissue using NB110-57188. Immunohistochemical staining of paraffinembedded human colon carcinoma, using NB500-106. Species: Hu Applications: FACS, IHC, WB Species: Hu, Mu, Rt Applications: FACS, ICC, IHC, IP, WB Species: Ch, Dr, Hu, Mu, Rt, Ye Applications: IHC, IP, WB PSA Antibody NB110-59960 PSA Antibody NB600-835 S100A6 Antibody NB100-1765 Immmunohistochemical staining of paraffinembedded human prostate carcinoma using NB110-59960. Species: Hu Applications: IHC-P, WB For research purposes only. Not for use in humans. Prices subject to change. Immunohistochemical staining of paraffin-embedded brain glioma tissue using NB1001765. Staining of formalinfixed, paraffinembedded human prostate carcinoma using NB600-835. Species: Hu Applications: IHC-P, WB Species: Hu Applications: ELISA, IHC, WB FAX: 303.730.1966 • WEB: www.novusbio.com 22 ORDERING U.S. ORDERING Phone: 303.730.1950 888.506.6887 Fax: 303.730.1966 Email: [email protected] Internet: www.novusbio.com EUROPEAN ORDERING Phone: +44 (0) 1223 426001 Fax: +44 (0) 871 971 1635 Email: [email protected] Web: www.novusbio.com TECHNICAL SUPPORT Phone: 303.730.1950 888.506.6887 Email: [email protected] Live Chat featured on our website, www.novusbio.com TOLL FREE: 888.506.6887 • PHONE: 303.730.1950 Daily product updates! www.novusbio.com