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Download Engineering E.Coli to see Light
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Engineering E.Coli to see Light Voigt CA et. al. 2005 Light Engineering Imaging using E.Coli System Stimulus Sensor Signal Transducer Effector Output Stimulus Red Light Highly defined edges Two-dimensional image Sensor Phytochrome Membrane bound extracellular sensor Intracellular regulator Fusion protein: phytochrome and EnvZ histidine kinase domain Extracellular sensor Intracellular regulator Photoreceptor Expression PCB not produced in E.Coli Introduction of PCB synthesis genes Converts haem into PCB Transduction Mechanism Red light represses EnvZ autophosphorylation Output OmpR-P activates promoter linked to reporter gene lacZ reporter produce black compound OmpR-P dependent ompC promoter System Revisited Review Created novel genetic circuit High definition film (~100 megapixels per square inch) lacZ expression as a function of light intensity / wavelength Transfer function Something for the team to think about!! Light Canon Characterisation Macro Resolution Micro Transfer function SigB activity as a function of: Intensity Wavelength
