Download Animal Study Proposal sample document

UNIVERSIDAD DE PUERTO RICO EN HUMACAO
CALL BOX 860, HUMACAO, PUERTO RICO 00792
TEL. (787) 850-0000 EXT 9388
Institutional Animal Care and Use Committee
Animal Study Protocol
Please Leave Blank
[This form was developed as an msWord table.
You can type your responses into the table fields. If field does
not apply, please type “does not apply”]
Protocol #:
Approval Date:
Date:
Expiration Date:
A. ADMINISTRATIVE DATA
Department:
Principal investigator:
Mailing address:
Phone:
Fax:
E-mail:
Project title:
Initial submission:
Renewal:
Modification:
Funding Source:
List the names of all individuals authorized to conduct procedures involving animals under this proposal and
identify key personnel [e.g., co-investigator(s)], providing their department, telephone, fax, and e-mail:
B. ANIMAL REQUIREMENTS
Genus:
[e.g., Mus]
Strain, subspecies, or
breed:
[e.g., C57BL/6]
Species:
[e.g., musculus]
Common
name:
[e.g., Black6]
Approximate age, weight or size:
Sex:
Source(s):
[e.g., name of vendor or breeder, or bred in-house]
Primary housing
location(s):
EXPIRATION DATE:
[Facility manager must certify in Section S that facility has the resource capability to
support the study. If animals will be housed in lab or anywhere else outside central
facility for more than 12 hours, provide building and room number.]
Location(s) where manipulation will
be conducted:
Number of animals to be used:
Year 1:
Year 2:
Year 3:
Total number of animals to be used:
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C. TRANSPORTATION
Transportation of animals must conform to all institutional guidelines/policies and federal regulations. If
animals will be transported on public roads or out of state, describe methods you will use to comply with
USDA regulations. If animals will be transported between facilities, describe the methods and containers that
will be used. If animals will be transported within a facility, include the route and elevator(s) that will be
used.
D. STUDY OBJECTIVES
Briefly explain the aim of the study and why the study is important to human or animal health, the
advancement of knowledge, or the good of society in language that a layperson can understand. Please
comment on whether the study unnecessarily duplicates other studies.
E. RATIONALE FOR ANIMAL USE
1. Explain your rationale for animal use. [The rationale should include reasons why it is necessary to use
animal models.]
2. Justify the appropriateness of the species selected. [The species selected should be the lowest possible
on the phylogenetic scale.]
3. Justify the number of animals to be used. [The number of animals should be the minimum number
required to obtain statistically valid results. Include justification for group size through a power analysis
when possible.]
F. DESCRIPTION OF EXPERIMENTAL DESIGN AND ANIMAL PROCEDURES


Briefly explain the experimental design and specify all animal procedures. All procedures to be employed
in the study must be described. This description should allow the IACUC to understand the experimental
course of an animal from its entry into the experiment to the endpoint of the study. A flowchart may be
an effective presentation of the planned procedure.
A best practice is to provide an acceptable range of the specific items described below to allow flexibility
in the use of professional judgment and avoid non-compliance due to work conducted off protocol as a
result of overly restricted parameters.
Include the following specific information, if applicable:









Animal identification methods [e.g., ear tags, tattoos, collar, cage card, implant, etc.].
Methods of restraint [e.g., restraint chairs, collars, vests, harnesses, slings, etc.]. Describe how
animals are restrained for routine procedures like blood withdrawals. Prolonged restraint must be
justified with appropriate oversight to ensure it is minimally distressing. Describe any sedation,
acclimation or training to be used.
Experimental injections or inoculations [substances, e.g., infectious agents, adjuvants, etc.; dose,
sites, volume, route, and schedule].
Blood withdrawals [volume, frequency, withdrawal site, and methodology].
Radiation [dosage and schedule].
Food or fluid restriction If food, or fluid, or both food and fluid, will be restricted, describe method for
assessing the health and wellbeing of the animals. [Amount earned during testing and amount freely
given must be recorded and assessed to assure proper nutrition.] If you are seeking a departure from
the recommendations of the Guide, provide a scientific justification.
Pharmaceutical-grade and Non-pharmaceutical-grade Compounds Identify any drugs, biologics,
or reagents that will be administered to animals. If these agents are not human or veterinary
pharmaceutical-grade substances, provide a scientific justification for their use and describe methods
that will be used to ensure appropriate preparation and administration.
Other procedures [e.g., survival studies, tail biopsies].
Resultant effects, if any, that the animals are expected to experience [e.g., pain or distress, ascites
production, etc.].
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
Other potential stressors [e.g., noxious stimuli, environmental stress] and procedures to monitor
and minimize distress. If a study is USDA Classification E, describe any non-pharmaceutical methods
that will be used to minimize pain and distress.
Experimental endpoint criteria [e.g., tumor size, percentage body weight gain or loss, inability to eat
or drink, behavioral abnormalities, clinical symptomatology, or signs of toxicity] must be specified when
the administration of tumor cells, biologics, infectious agents, radiation or toxic chemicals are expected
to cause significant symptomatology or are potentially lethal. List the criteria that will be used to
determine when euthanasia is to be performed. Death as an endpoint must be scientifically justified.
Veterinary care Indicate the plan of action in case of animal illness [e.g., initiate treatment, call
investigator prior to initiating treatment, euthanize].
Surgical procedures [provide details of survival and non-survival surgical procedures in Section G.].



G. SURGERY
If surgery is proposed, complete the following:
1.
Identify and describe the surgical procedure(s) to be performed. Include preoperative procedures [e.g.,
fasting, analgesic loading], and monitoring and supportive care during surgery. Include the aseptic
methods to be used.
2.
Identify the individual(s) that will perform surgery and their qualifications, training, and/or experience.
3.
Identify the location where surgery will be performed. [building(s) and room(s)]
4.
If survival surgery, describe postoperative care that will be provided and frequency of observation.
Identify the responsible individual(s) and location(s) where care will be provided. [building(s) and
room(s)] Include detection and management of postoperative complications during work hours, after
hours, weekends and holidays.
5.
If non-survival surgery, describe how euthanasia will be provided and how death will be determined.
6.
Are paralytic agents used during surgery? If yes, please describe how ventilation will be maintained
and how pain will be assessed.
7.
Has major or minor survival surgery been performed on any animal prior to being placed on this study?
[Major survival surgery penetrates and exposes a body cavity or produces substantial impairment of
physical or physiologic functions or involves extensive tissue dissection or transection (such as
laparotomy, thoracotomy, craniotomy, joint replacement, or limb amputation)]. If yes, please explain.
8.
Will more than one survival surgery be performed on an animal while on this study?
If yes, please justify.
H. PAIN OR DISTRESS CLASSIFICATION AND CONSIDERATION OF ALTERNATIVES
1.
Pain or distress classification for USDA covered species. See Appendix 1 for classification definitions and
examples.
2.
Attachment 1, Explanation for USDA Classification E, must be completed for animals listed in
Classification E.
Species
(common
name)
USDA
Classification*
B, C, D or E
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Number of animals used each
year
Year 1
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Year 2
Year 3
3 years total
number of
animals
3
Total number of animals
3. Consideration of Alternatives
If any procedures fall into USDA's Classification D or E, causing more than momentary or slight pain or
distress to the animals, describe your consideration of alternatives and your determination that alternatives
are not available. Delineate the methods and sources used in the search. Database references must include
databases searched, the date of the search, period covered, and the keywords used. Alternatives include
methods that:



refine existing tests by minimizing animal distress,
reduce the number of animals necessary for an experiment, or
replace whole-animal use with in vitro or other tests.
If you use ascites production to produce antibodies, you must provide the reason for not using an in vitro
system. Note that you must certify in Section Q.5. that no valid alternative was identified to any described
procedures which may cause more than momentary pain or distress, whether relieved or not.
I. ANESTHESIA, ANALGESIA, TRANQUILIZATION, OTHER AGENTS
For animals indicated in Section H.1. Classification D, specify the anesthetics, analgesics, sedatives or
tranquilizers that will be used. [A best practice is to provide an acceptable range of the specific items to allow
flexibility in the use of professional judgment and avoid non-compliance due to work conducted off protocol
as a result of overly restricted parameters.] Include the name of the agent(s), the dosage range, route(s)
and schedule of administration. If information is provided in Section R.5., above, please cross-reference.
Describe tracking and security of controlled drugs (Drug Enforcement Agency requirements).
J. METHOD OF EUTHANASIA OR DISPOSITION OF ANIMALS AT END OF STUDY
Indicate the proposed method of euthanasia. If a chemical agent is used, specify the dosage range and
route(s) of administration. If the method(s) of euthanasia include those not recommended by the AVMA
Guidelines on Euthanasia [e.g., decapitation or cervical dislocation without anesthesia], provide scientific
justification as to why such methods must be used. Indicate the method of carcass disposal if not described
in Section K. below.
K. HAZARDOUS AGENTS
Use of hazardous agents requires the approval of the institutional Biosafety Office/Committee. Attach
documentation of approval for the use of recombinant DNA or potential human pathogens.
Hazardous Agent
Yes
No
Agent
Date of Biosafety
Approval
Tracking #
Radionuclides
Biological Agents
Hazardous Chemicals or
Drugs
Recombinant DNA
Study Conducted at Animal Biosafety Level:
1
2
3
4
Describe the practices and procedures required for the safe handling and disposal of contaminated animals
and material associated with this study. Also describe methods for removal of radioactive waste and, if
applicable, the monitoring of radioactivity.
Additional safety considerations:
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L. BIOLOGICAL MATERIAL/ANIMAL PRODUCTS FOR USE IN ANIMALS
[e.g., cell lines, antiserum, etc.]
1. Specify Material:
2. Source:
Material Sterile or Attenuated:
Yes
No
Has the material been tested for pathogens? (e.g., MAP - Mouse Antibody Production; RAP - Rat
Antibody Production; HAP - Hamster Antibody Production, PCR test)
Yes
[Attach copy of results]
No
3. I certify that the tested materials to be used have not been passed through rodent species outside
of the animal facility in question and/or the material is derived from the original tested sample. To
the best of my knowledge the material remains uncontaminated with rodent pathogens.
Initials of Principal Investigator
M. FIELD STUDIES
1. If renewal of an approved IACUC Protocol: Please provide Protocol# and a brief summary (a few
sentences) describing work accomplished during the last approval period and how the work proposed in
this renewal extends knowledge from the previous studies.
2. Characteristics of Wild Animals and Pain Category of Research: List and describe the animals to
be studied. Indicate the anticipated number of animals to be used in each Pain Category of Research and
the total number of animals involved during the 3-year approval period of this protocol. Indicate whether
the planned procedures consist of animals being bred, conditioned, or held for use in teaching, testing,
experiments, research, or surgery, but not yet used for such purposes (USDA Pain Classification B),
produce momentary, slight, or no pain, discomfort or distress (USDA Pain Classification C), more than
momentary or slight pain, discomfort or distress which is alleviated by the use of appropriate anesthetics
and/or analgesics (USDA Pain Classification D), or pain, discomfort, or distress, which cannot, or is not
alleviated by the administration of appropriate anesthetics and/or analgesics (USDA Pain Classification
E). (Note: The total number of animals requested here must be justified in response to item #6 below.
The IACUC recognizes that field studies often involve many species, some of which may be unanticipated
or even unknown to science before the onset of the study. The IACUC recognizes that it is not always
possible to predict at the initiation of field studies the number of animals to be encountered, the species
to be encountered, or the effects of research procedures on each species. The IACUC recognizes that
pain perception by many species of vertebrate animals may not be uniform over the various portions of
their bodies, and that broad extrapolation of pain perception across taxonomic lines may not be
appropriate.)
Taxa or Species
Characteristics
(age, sex,
weight)
#used in Pain
Classification B
Procedures
#used in Pain
Classification C
Procedures
#used in Pain
Classification D
Procedures
#used in Pain
Classification E
Procedures
Total
Number
Anticipated
3. Off Campus Study Location: Describe below the anticipated off campus location(s) where the field
study will be performed. If this protocol is to be conducted at another academic institution, or a
zoological garden, aviary, aquarium, or oceanarium, please name that institution and attach a letter with
official letterhead from that institution that indicates that they are anticipating the presence of this
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research protocol, and whether they have an assurance on file with the PHS. (Note: The IACUC
recognizes that it is not always possible to predict at the initiation of field studies all potential observation
or collection opportunities. PIs must inform the IACUC of new location opportunities as they develop by
submitting a completed Request for Minor Amendments for Animal Studies of an Approved Protocol. If
collaborating institutions will supply live animals collected by their staff, or will perform portions of this
protocol, or University faculty or staff will conduct this protocol at another institution, those institutions
must be declared here.)
4. Permits: The IACUC recognizes that you should have knowledge of all regulations pertaining to the
animals under study, including whether they are considered endangered or threatened, and will obtain all
applicable permits before initiating the study. Therefore, indicate if federal, state, and/or local permits
are required, whether they have been requested, and evidence they been obtained accordingly whenever
applicable. (Note: The IACUC recognizes that state and federal wildlife and forest agencies review
applications for permits for their scientific merit and their potential impact on native populations, and
issue permits that authorize the taking of specified numbers of individuals, the taxa and methods
allowed, the period of study, the type of activities allowed in protected lands, and often other restrictions
designed to minimize the likelihood that an investigation will have deleterious effects.)
5. Justification for the Use of Animals: If animals in the wild will be used, briefly state in lay terms the
purpose and scope of work (i.e., research hypotheses or teaching objectives), and the procedures
(general sequence of events) in which animals will be involved as to clearly describe how they will be
observed, any interactions with the animals, whether the animals will be disturbed or affected, and any
special procedures anticipated. (Note: Your response must be in language that a high school senior can
understand.)
6. Briefly describe the rationale, prior experience, statistical analysis, or other methods used to understand
the population status of the taxa or species to be studied, and used to determine the anticipated total
number of animals that will be encountered or involved during the 3-year approval period of this
protocol. (Note: The total number of animals requested in item #2 above must be justified here. The
IACUC recognizes that it is not always possible to accurately predict at the initiation of field studies the
number of animals to be encountered. The minimum number of animals necessary for accomplishing the
goals of the study should be used.)
7. Will this study only consist of the direct, unobtrusive observation of free-ranging animals under natural
conditions or conditions described in item #3 above (i.e., at another academic institution, or a zoological
garden, aviary, aquarium, or oceanarium), and not require that animals be contacted, capture or
restrained at anytime?
Yes
No
If yes, stop here. Section M. Field Studies is complete. If No, proceed to item #8.
8. Will the captured animals ever be transported to the University?
Yes
No
If yes, name the University building and room number where animals will be taken.
If no, proceed to item #9.
9. Capture & Restraint: Briefly describe the technique(s) of wild animal capture, and the method(s) and
duration of animal restraint that will be used. (Note: Techniques that have minimal impact on the
animal, require the shortest period of time to accomplish, reduce hazards to research personnel, and are
environmentally benevolent should be used whenever possible.)
10. Will the method of wild animal capture or restraint cause more than momentary discomfort, pain, or
distress to the animals?
Yes
No
If yes, briefly describe the methods to be used that will assist in avoiding or alleviating the potential for
animal distress, pain, or discomfort. If drug-induced sedation, analgesia, or anesthesia will be used,
list the drugs, and the dose and route of administration.
If no, proceed to item #11.
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11. Are animals euthanized immediately at the moment of capture for preparation as museum
specimens, for post mortem tissue collection, or for other purposes? (Note: If animals are euthanized
later at the conclusion of study, and not immediately following their first capture, respond “No” here.)
Yes
No
If yes, the IACUC recognizes that the PI must have Indicated the proposed method of euthanasia in
Section J. above. In addition, if tissues are to be collected post mortem, list the tissues to be collected.
STOP here, Section M. Field Studies is complete.
If no, proceed to item #12.
12. Marking: Briefly describe the marking technique that will be used, the nature and duration of restraint
required during marking, the amount of tissue affected by the technique, and whether the method of
marking will cause animals momentary or prolonged distress. (Note: If drug-induced sedation,
analgesia, or anesthesia will be used during the marking of animals, list the drugs, and the dose and
route of administration.)
13. If the preferred marking technique may result in animals that will be at greater than normal risk of
infection, predation, or survival, or will have reduced reproductive fitness, justify below why this marking
technique must be used, and why other techniques that have less impact on the animal may conflict with
the purposes of this research activity.
14. Specimen Collection, Ante Morten: If biosamples will be collected from the captured animals prior to
their release, please list the tissues or specimens (e.g., tissues, blood, spleen, liver, lymph node, body
fluid) that will be collected ante mortem from animal(s), and indicate the amount of tissue to be
collected, the frequency of collection, and the method that will be used to collect the tissue sample (e.g.,
needle aspiration, punch biopsy, or surgical excision). In addition, describe the method of restraint, and
whether tranquilizers, sedatives, or anesthetics will be used, and their dose and route. Finally, describe
how anesthesia will be induced and maintained, including the dose and route of agents used, whether
post-operative/procedural analgesics will be used, their dose and frequency of administration, and a
description of the post-procedural methods of minimizing and/or alleviating pain and discomfort.
15. Animal Maintenance and Captive Care—Natural Settings: if animals are confined or restricted to an
enclosure in their natural setting for longer than 24 hours, indicate the period of time that animals will be
cared for in the field, describe the enclosure(s) that will be used, the methods for maintaining
appropriate living conditions that contribute to the animal’s health and well-being, including their diet
and frequency of feeding if applicable, and how environmental conditions will be controlled. In addition,
describe the factors that will be monitored to ensure that these methods of animal maintenance in the
field contribute to the health and well-being of the confined animals (e.g., appearance, behavior, activity,
growth). (Note: Methods must accommodate salient features of the animal’s ecology, morphology,
physiology, and/or behavior, and contribute to their health and well-being.)
16. Animal Maintenance and Captive Care—Laboratory Settings: if animals are transported to, and
housed within an enclosure in a University animal facility, laboratory, or other area on campus, indicate
to where the captured animals will be transported, including the University building and room number if
applicable, and the period of time that the animals will be cared for at this new location. While animals
are housed on campus, describe the enclosure(s) that will be used, the methods for maintaining
appropriate living conditions that contribute to the animal’s health and well-being, including their diet
and frequency of feeding, and how environmental conditions will be controlled. In addition, describe the
factors that will be monitored to ensure that these methods of animal maintenance in the laboratory
contribute to the health and well-being of the confined animals (e.g., appearance, behavior, activity,
growth). (Note: Methods must accommodate salient features of the animal’s ecology, morphology,
physiology, and/or behavior, and contribute to their health and well-being.)
17. Specific Questions on the Final Disposition of the Animals After Maintenance and Captive Care
Period:
a. Will the animals be released at the original site of capture, without impairment of their ability to
survive, while environmental conditions are conducive to their survival, and while their release is
not likely to spread pathogens? If yes, please explain your rationale.
b. If no, will the animals be humanely euthanized as described in response to item #11, above, if
applicable? If no, describe the final disposition of the animals in accordance to Section J above
and STOP, Section M. Field Studies is complete.
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N. SPECIAL CONCERNS OR REQUIREMENTS OF THE STUDY
List any special housing, equipment, animal care or any departures from the Guide [e.g., special caging,
water, feed, waste disposal, environmental enrichment, etc.].
O. PRINCIPAL INVESTIGATOR CERTIFICATIONS
1. I certify that I have attended the institutionally required investigator training course.
Year of Course Attendance:
Location:
2. I certify that I have determined that the research proposed herein is not unnecessarily duplicative of
previously reported research.
3. I certify that all individuals working on this proposal who are at risk are participating in the institution's
Occupational Health and Safety Program.
4. I certify that the individuals listed in Section A. are authorized to conduct procedures involving animals
under this proposal, have attended the institutionally required investigator training course, and received
training in: the biology, handling, and care of this species; aseptic surgical methods and techniques (if
necessary); the concept, availability, and use of research or testing methods that limit the use of animals
or minimize distress; the proper use of anesthetics, analgesics, and tranquilizers (if necessary); and
procedures for reporting animal welfare concerns.
5. For all USDA Classification D and E proposals (see section H.1.): I certify that I have reviewed the
pertinent scientific literature and the sources and/or databases as noted in Section H.2. and have found
no valid alternative to any procedures described herein which may cause more than momentary pain or
distress, whether it is relieved or not.
6. I certify that I will obtain approval from the IACUC before initiating any significant changes in this study.
7. I certify that I will notify the IACUC regarding any unexpected study results that impact the animals. Any
unanticipated pain or distress, morbidity or mortality will be reported to the attending veterinarian and
the IACUC.
8. I certify that I am familiar with and will comply with all pertinent institutional, state, and federal rules
and policies, including the Guideline of the American Society of Mammalogists for the Use of Wild
Mammals in Research, the Guidelines for Use of Fishes in Research, the Guidelines for Use of Live
Amphibians and Reptiles in Field and Laboratory Research, the Guidelines to the Use of Wild Birds in
Research, PHS policy, AWA, The Guide, and AAALAC guidelines when applicable.
Principal Investigator
Name:
Signature:
Date:
P. CONCURRENCES
PROTOCOL NUMBER _____________ (leave blank)
Supervisory concurrence as applicable:
Name:
Signature:
Date:
Safety Office/Committee Certification of Review and Concurrence:
[Required of all studies that use hazardous agents.]
Name:
Signature:
Date:
Facility Management/Veterinarian certification of resource capability in the indicated facility to support the
proposed study:
Facility:
Name:
Signature:
Date:
Facility:
Name:
Signature:
Date:
Comments:
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Attending Veterinarian certification of review and consultation on proper use of anesthetics and pain relieving
medications for any painful procedures:
Name:
Signature:
Date:
[IACUC Office: add any additional concurrences that are needed e.g., radiations safety, Drug Enforcement
Agency licensure, select agents]
Q. FINAL APPROVAL
Certification of review and approval by the Institutional Animal Care and Use Committee:
Name:
Signature:
Date:
List any attachments here:
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Appendix 1 - USDA Classifications and Examples
Classification B: Animals being bred, conditioned, or held for use in teaching, testing, experiments,
research, or surgery, but not yet used for such purposes.
Examples:
 Breeding colonies of any animal species (USDA does not require listing of rats, mice, birds) that are
handled in accordance with IACUC approval, the Guide and other applicable regulations. Breeding
colony includes parents and offspring.
 Newly acquired animals that are handled in accordance with IACUC approval and applicable
regulations.
 Animals held under proper captive conditions or wild animals that are being observed.
Classification C: Animals upon which teaching, research, experiments, or tests will be conducted involving
no pain, distress, or use of pain-relieving drugs.
Examples:
 Procedures performed correctly by trained personnel such as the administration of electrolytes/fluids,
administration of oral medication, blood collection from a common peripheral vein per standard
veterinary practice [dog cephalic, cat jugular] or catheterization of same, standard radiography,
parenteral injections of non-irritating substances.
 Manual restraint that is no longer than would be required for a simple exam; short period of chair
restraint for an adapted nonhuman primate.
Classification D: Animals upon which experiments, teaching, research, surgery, or tests will be conducted
involving accompanying pain or distress to the animals and for which appropriate anesthetic, analgesic, or
tranquilizing drugs will be used.
Examples:
 Surgical procedures conducted by trained personnel in accordance with standard veterinary practice
such as biopsies, gonadectomy, exposure of blood vessels, chronic catheter implantation, and
laparotomy or laparoscopy.
 Blood collection by more invasive routes such as intracardiac or periorbital collection from species
without a true orbital sinus [e.g., guinea pigs].
 Administration of drugs, chemicals, toxins, or organisms that would be expected to produce pain or
distress but which will be alleviated by analgesics, anesthetics, tranquilizers, or supportive care.
Classification E: Animals upon which teaching, experiments, research, surgery, or tests will be conducted
involving accompanying pain or distress to the animals and for which the use of appropriate anesthetic,
analgesic, or tranquilizing drugs will adversely affect the procedures, results, or interpretation of the
teaching, research, experiments, surgery, or tests.
Examples:
 Procedures producing pain or distress unrelieved by analgesics such as toxicity studies, microbial
virulence testing, radiation sickness, and research on stress, shock, or pain.
 Surgical and postsurgical sequella from invasion of body cavities, orthopedic procedures, dentistry or
other hard or soft tissue damage that produces unrelieved pain or distress.
 Negative conditioning via electric shocks that would cause pain in humans.
 Chairing of nonhuman primates not conditioned to the procedure for the time period used.
NOTE REGARDING CLASSIFICATION E: An explanation of the procedures producing pain or distress in
these animals and the justification for not using appropriate anesthetic, analgesic or tranquilizing drugs must
be provided on Attachment 1. This information is required to be reported to the USDA, will be available
from USDA under the Freedom of Information Act (FOIA), and may be publicly available through the Internet
via USDA’s website.
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Attachment 1 - Explanation for USDA Classification E
[This report is required to accompany USDA Form 7023 to support any USDA Classification E listings.]
This document must be typed.
Name of investigator:
Animal study proposal title:
Species and number of animals listed in Classification E for each year:
Species:
Number of animals:
year 1 year 2 year 3 Total:
Description of project including reason(s) for species selection:
Provide a scientific justification to explain why the use of anesthetics, analgesics, sedatives or tranquilizers
during and/or following painful or distressing procedures is contraindicated:
Signature of investigator:
Date:
Signature of IACUC Chairperson:
Date:
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