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Protists I Lab 3 BIOL 171 Remember!: Classification System We’ll be looking at all of these! Ancestral Eukaryote Protists are everywhere in Eukarya! “the junk drawer of the eukaryotes” We’ll be looking at all of these! Ancestral Eukaryote Protists are everywhere in Eukarya! “the junk drawer of the eukaryotes” 6 Kingdoms • • • • • • Plants (Plantae) Animals (Animalia) Fungi (Fungi) Eubacteria Archaeabacteria Protista A constantly changing system… Linnaeus Haeckel (1735) (1866) 2 kingdoms 3 kingdoms Animalia Vegetabilia Chatton (1925) 2 groups Animalia Plantae Eukaryote Copeland Whittaker Woese  (1938) (1969) (1977,1990) 4 kingdoms 5 kingdoms 3 domains Animalia Animalia Plantae Plantae Protoctista (not treated) Protista Prokaryote Monera Fungi Eukarya Protista Monera Archaea Bacteria Lab Study A: Euglenozoans – Trypanosoma levisi (prepared slide) – Euglena (make wet mount) – not in manual (use depression slide) – Termites (Trichonympha) - procedure not in manual Trypanosoma and red blood cells Euglena Trichonympha • Lives in the intestine of the termite • Bacterial endosymbionts inside Trichonympha digest cellulose - Termite > Trichonympha > Spirochetes Procedure 1. 2. 3. 4. 5. 6. Place a couple of drops of Ringer’s solution on a clean slide. Transfer a termite into the drop of solution. Place slide under a dissecting microscope. Place the tips of dissecting needles at either end of the termite and pull in opposite directions. Locate the long tube that is the termite’s intestine. Place a cover slip over the specimen and lightly press down on coverslip to release the Trichonympha from the intestines. Observe with a compound microscope. Lab Study B: Alveolates Ciliate: Paramecium caudatum – (wet mount) in manual Dinoflagellates: mixed dinoflagellates (live & wet mount), and Peridinium (wet mount) not in manual Paramecium structures Dinoflaggelates Lab Study C: Stramenopiles Diatoms (Bacillariophyta) – make wet mount Also observe diatomaceous earth (the cell wall deposits from diatoms) – make wet mount and look at prepared slides Diatom diversity Diatom cell wall made of silica Stramenopile flagella Brown Algae (Phaeophyta) Living: Ectocarpus and Sphacelaria Preserved: Fucus and Laminaria Lab Study D: Rhizaria (different title from manual) • Foraminiferans - prepared slides Radiolarians - prepared slides Think about… • • • • • • Morphological characteristics Ecology of the organism How does the organism get around? What role do they play in the ecosystem? Do they have any economic value? Where do they live? • Don’t know the answer?? It’s probably a great research question! Ask me about it.