Download DNA Extraction Introduction Slides

What is DNA?
• DeoxyriboNucleic Acid
• hereditary material
– National Institutes of Health
• a complex molecule which contains the biological instructions
necessary to build and maintain an organism and that makes each
organism unique
– genome.gov/nature.com
• present in all known living organisms (bacteria, insects, plants,
animals) and some viruses
• starting material for many bioinformatics questions
DNA in the News – Jan 20, 2016
• “DNA Reveals Far-Off Origins of Ancient ‘Gladiators’”
–
National Geographic
• “Through DNA, family finds missing women’s remains, closure”
–
Fox News
• “Life-sized models of face 3D-printed with DNA technology”
–
The Guardian
• “Researchers investigating how led exposure could affect DNA”
–
Michigan Radio
• “Searching for cancer maps in free-floating DNA”
–
NYTimes
DNA Extraction
Objective:
• Extract and purify intact DNA from cells
• Almost every cell in your body
contains DNA!
Steps:
1. Collect cells (cheek swab, blood,
tissue…)
2. Break open cells (cell lysis)
3. Remove proteins and isolate DNA
DNA