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Biological and nutritional value of cereals and legumes Sanitary-hygienic expertise of bread Ph.D Maksimenko L.V. Cereals (maize, rice, wheat) are the main power source for the grate bulk of the population all over the world Cereals provide about 350 kcal per 100 grams Cereals contribute 70 - 80 % of the total energy intake, and more than 50 % of protein intake in typical Indian diets. The endosperm is composed mostly of starch, the outer pericarp aleurone layer and germ contain most of the essential nutrients. Biological value: nutritional composition of cereals and legumes Product Carbohydr Caloration, ides, % kcal Protein, % Oil,% K Ca Mg P Fe Soybean 28,1 17 23 368 1797 217 280 704 15,7 Peas Haricot bean 19,3 2,2 49,8 304 906 63 107 369 4,7 19,2 1,9 50,3 303 1061 157 167 504 6,7 Lentils 18 1,6 49,8 301 607 55 69 347 6 Wheat hard bread 11,4 1,7 62,4 318 363 34 137 357 3,4 Oats 10,8 6 61,1 351 117 135 361 5,5 Wheat soft bread 9,7 1,5 63,1 312 316 42 140 316 3,7 Barley 9,5 1,5 72 348 477 43 141 400 4,1 9 1,5 64,6 316 424 59 120 366 5.4 Maize 8,3 4,2 63,6 334 209 7 82 186 2,2 Rice long 7,1 0,7 79 365 115 28 25 115 0,8 Rice round 6,6 0,6 79 317 86 9 35 108 0,8 Rye 421 Cereal proteins • The negative feature is amino acide imbalance: • Cereal proteins are deficient in the essential amino acid lysine. • Maize proteins are deficient in lysine and tryptophan (a precursor of niacin= vitamin PP). • Some strains of maize contain an excess of leucine which interferes with the conversion of tryptophan into niacin and thus aggravates the pellagragenic action of mais. Legume proteins • • • • • • • Proteins content: Legumes - 20-40% fish (21,5%), meat (20%), eggs (13,3%), cereals (10%). Aminoacide composition of legumes proteins is close by aminoacide composition of animal protein (but incompletely). • Legume proteins are poor in methionine and to a lesser extent in cystein. • On the other hand they are rich in lysine. • If cereals are eaten with pulses, as in common in the traditional Indian diets, cereals and pulse proteins complement each other and provide a more balanced and “complete” protein intake. • • • • • • • Vegetable oils Cereals - 0,5-2 % (corn contains a little bit more - 4,5-5 %). Soy (17 %) and oil-bearing crops (sunflower, peanut, cocoa, olive and etc.) are exclusion. Oils are fats that are liquid at room temperature, like the vegetable oils used in cooking. Oils come from many different plants and from fish. Oils are NOT a food group, but they provide essential nutrients. Oils consist of polyunsaturated fatty acids including essential fatty acids (linoleic, linolenic and arachidonic acids). Linoleic acid (18: 9, 12) is present in wheat, peanut, seeds of a cotton plant, soy and etc. Linolenic acid (18: 9, 12, 15) together with a linoleic acid is present in cereals oil, specially in linseed-oil. Arachidonic acid (20: 5, 8, 11, 14) is present in combination with a linoleic acid, specially in ground-nut oil. The polyunsaturated fatty acids in a structure of cellular membranes phospholipides provide • cellular membranes flowability. • reduction of cholesterol in a blood plasma and, as suppose, prevent coronary artery disease. Carbohydrates • An animal organism is able to synthesize a number of carbohydrates from fat and protein, but the large part of carbohydrates enters by phytogenic food. • The content of hydrocarbons in the grain is 60-75%, bean - no more than 50%. • Grain and beans carbohydrates are submitted by amylum (starch) and dietary fibers. • Starch, as against monosaccharides and disaccharides, is assimilated step-by-step, that provides an organism by monosaccharides during a prolonged period and, thus, eliminates development of a nutritional glucosuria. The development of glycosuria is possible for excess consumption of sugar (saccharose). • The dose 50-100 grams in a day prevents a ketosis and loss of myoproteins. •Optimum ratio of different carbohydrates in a diet: •64 % starch, •36 % - saccharides. • Carbohydrates are indispensable to normalization of protein and fat metabolism. At carbohydrate starvation there are ketones in urine (ketons are the products of partial oxidation of protein and fats), that leads to acidosis. • • • • • Dietary fibers (DF) DF (biopolymer molecules) are components of vegetative cell wall and not assimilated or partially assimilated since human dietary system does not contain hydrolyzing enzymes for disintegration of (14)-glycosidic linkages. – Unassimilated : cellulose, hemicellulose (xylans) and lignin – Partially assimilated: glucopolicaccharides (resin, pectins and pentosans). The main sources of DF is a buckwheat barley, bread of coarse grinding or rye, wheaten bran, crude fruit and vegetables (beet, turnip, rutabaga, carrots). Content of DF: • Fruit and the vegetables (except for potato) contain 32 % (in general as pectin and resin), • Grain as a whole - 29 % (pulp and lignin), • Bread - 16,8 %, • Blended dishes - 12,6 %, • Cereal food (porridge, boiled rice, buckwheat, etc.) - 7,1 %. Hygienic recommendations for DF intake: 15-40 gr in a the daily diet (at more than 35 years old – not less 24 g ). Diseases associated with inadequate intake of dietary fibers (less 14 g per 1,000 kcal of food) : disbacteriosis (pectin stands up against putrefactive bacterias), atherosclerosis, sugar diabetes, rectal cancer, diverticulosis and constipation , poisoning by the endo-and exotoxin (heavy metals, radionuclides) Vitamins • Legumes are rich in minerals and B-group vitamins • Fermentation modifies the nutritive value of legumes in that the vitamin content particularly that of riboflavin, thiamine and niacin is enhanced. • Germinating pulses, however, contain higher concentration of vitamins, especially vitamin C and B vitamins. • In the dry state, legumes do not contain vitamin C. • The highest concentration of vitamin A is found in sweet potatoes; just one mediumsized baked sweet potato contains more than 28,000 international units (IU) of vitamin A, or 561% of your recommended daily value (DV). Beef liver, spinach, fish, milk, eggs, and carrots also are good sources. Beta-carotene ( Vitamin A): carrots, squash, pumpkin, sweet potato, dark green leafy vegetables (spinach and broccoli), red peppers, tomatoes, and yellow fruits such as apricots, mango and peaches. Vitamin B1 Thiamin: brown rice, wholemeal bread, fortified flour and breakfast cereals, pulses, nuts, potatoes and yeast extract. Vitamin B2 Riboflavin: milk and dairy products, eggs, cereals, wholemeal bread, rice, yeast extract, green leafy vegetables (broccoli and spinach), mushrooms and beverages such as beer, lager and tea. Vitamin B3 Niacin: wholegrain and fortified cereals, maize, fortified flour, yeast extract, coffee beans and beverages such as beer and lager. Vitamin B6 Pyridoxine: wholegrains such as brown rice, oatmeal, and wholemeal bread, fortified cereal products, potatoes, bananas, soya beans, nuts, pulses, yeast extract and beverages such as beer and lager. Vitamin B12 Cobalamin: milk, dairy products and eggs; fortified plant foods include soya milk, breakfast cereals, veggieburger mixes, yeast extract and herbal soft drinks. : cereal products, potatoes, pulses (e.g. chickpeas, black-eyed beans), leafy green vegetables (e.g. broccoli), nuts, yeast extract and fruits such as oranges and bananas. Vitamin C (Ascorbic Acid): citrus fruits, strawberries, guava, berries, currants, fruit juice, potatoes and nuts; vegetables (cabbage, cauliflower, broccoli, spinach, kale and green peppers) - are rich sources but large amounts of the vitamin are lost during food storage, preparation and cooking. Vitamin D: synthesised from sunlight and also found in eggs, fortified margarines, breakfast cereals and soya milk. Vitamin E: margarine and spreads, foods high in fat such as crisps, vegetable oils such as corn, soya and sunflower but not olive oil and small amounts in dairy products. Vitamin K: dark leafy greens such as cabbage, kale, spinach and broccoli, vegetable oils such as rapeseed, soybean and olive, but not corn or sunflower oil. Smaller amounts are found in eggs and dairy products. The content of vitamins in typical Indian cereal Notice: Bengal Gram contents 3 mg of vitamin C per 100 g. Grams Millets Pulses Cereals Thiamin e, mg Raw rice 0.06 Wheat whole Maize Jowar Bajra Ragi Bengal Gram Black Gram Niacin, mg 1,9 Riboflav in, mg 0.06 0.45 5,0 0.17 0.42 0.30 0.30 0.20 0.30 1,8 3,1 2,3 2,3 2,9 0.1 0.1 0.25 0.18 0.15 0.42 2,0 0.20 Kinds of bread • Pan bread: flour, water (milk), yeast, eggs, sugar and salt. • Distinguish between the crust and crumb: crumb has a porous structure and is formed through the formation of carbon dioxide as a result of yeast vital activity at dough maturation (1-4 h before baking). • Floor troughs, flat bread: flour, water . Kinds of Russian bread • White (wheat, threaded) bread is baked from fine wheat flour. • Grey bread is baked from a mixture of wheat and rye flour, or wheat rough flour, or wheat flour with added bran. • Black (ray) bread is baked from rye flour. Bread • Bread gives 40 % of the energy value of the daily diet (100 g of bread = 200-250 kcal). • At consumption of 500-600 g of bread per day 35-40 g of protein and 230-260 g of carbohydrates are provided • Content of bread: • • • • • carbohydrates (40-45 %), proteins (6-8 %), fats (1-1,5 %), vitamins PP, B1, a little quantity of B2, a lot of K, Fe, P, Mg and S. • Protein composition depends on the grain. • . Anti-nutritional factors • Legumes have some anti-nutritional factors such as phytates and tannins which adversely affect the availability of some nutrient. • Most of the anti-nutritional factors are destroyed by heat. Nutritional value and digestion of bread depends on a kind and flour extraction rate at the grinding • Flour extraction rate → 95% • (coarse grinding): the flour is dark, rough, but high in B vitamins and fiber • The bread is recommended for: • • healthy to prevent hypo-and avitaminosis of B-group, • postoperative patients to stimulate intestinal motility and rapid detox, • patients with diabetes mellitus, atherosclerosis and obesity. Flour extraction rate → 10% (wheat), 50% (ray) (fine grinding): the flour is white, light: easy to digest, provides a lot of energy. The bread is recommended for: • • • • • children, convalescents, malnourished and starving, TB-patients, patients after surgery for gastrointestinal tract, • patients with gastritis and gastric and duodenal ulcer. Nutritional value of bread • Proteins assimilation depends on the degree of grinding: – 74-85 % - coarse grinding, – 92 % - fine grinding. • Digestion of carbohydrates of bread is high (93-98 %) in any cases. • Vitamins. Only 10-20 % of vitamins are lost at baking. Therefore it is possible to consider bread as a valuable source of thiamin (0,15-0,25 mg%), riboflavin (0,1-0,15 mg %) and niacin (0,7-3 mg %). Epidemiological risk Potato disease - condition called “rope” • «Potato disease» of bread is conditioned by potato bacillus (Bacillus mesentericus). • Bacillus mesentericus is sporiferous bacteria, looks like a thin rod (0,5-0,6 3-10 micron). The vegetative cells are mobile and Gram-positive. • Its settlements are yellow - brown, dry, rogues. • Spores, falling with a flour or yeast into the paste, do not perish at bread baking and vegetate at high humidity and temperature at bread storage (usually in a summer in a plastic bag). A condition conducive to high moisture of bread is a violation of baking temperature regime. • Bread-crumb becames slimy, the mucus color is transparent - brown, the odor is offensive with under tint of molding fruit or anise. • Bacillus mesentericus is pathogenic for animal and person. • The bread with potato disease is unsuitable (the bread should be destroyed on site and bakery equipment to be decontaminated from spores also). Damage of bread by pigmentforming bacteria «wonderful rod» (Serration marcescens or Bacillus prodigious=“prodig” bacteria) • Rich colored slimy spots are on bread surface. • S. marcescens is a Gram-negative, rod-shaped, facultative anaerobic bacterium in the family Enterobacteraciae • Conditions are source of contamination, humidity, temperature. Serratia can be difficult to eradicate completely. • Bacillus prodigious is conditionally pathogenic for the person and animal also can fester. • Such bread is not suitable for consumption in view of changed organoleptic tags and potential health hazard. • Numerous historical incidents recount that the blood red pigment produced by S. marcescens growing in bread was interpreted as a sign of blood. Bread molding • Bread moulding is conditioned by funguses Aspergillius (white mould), Penicillium (green mould) and Mucor mucendo (mucor, smut). • Basic health risk is due to the accumulation of aflatoxins in bread. • The reason is the contamination of poorly dried grain by fungal spores (aflatoxins accumulate in the moldy grain or in moldy bread during the storage in humid conditions). • Aflatoxins are hepatotoxins and hepatocarcinogens. • MPC = 0.01 mg of aflatoxin B1/ kg Contamination of bread by fungi Fusarium • Fuzarium forms trichothecene mycotoxins contaminating overwintered in a field grain (harvest), or if the crop harvested at the rain. • Fuzario-toxins cause damage of bone marrow stem cells (poison by “drunk bread”, aseptic angine, alimentary toxic aleukia-ATA). Alimentary Toxic Aleukia (ATA) • ATA symptoms include fever, rashes, and bleeding from the nose, throat and gums. More severe symptoms include extreme leucopenia, and the elimination of bone marrow. • Fusarium sporotrichioides is a very uncommon species and is found to grow mostly in temperate regions on cereal crops but studies have found that the toxin production is most favorable at low temperature. • The presence of toxin-producing Fusarium sporotrichioides in the grain in Russia is believed to have caused the deaths of at least 100,000 people who consumed the toxic bread baked from overwintered in field and Fusarium-infected wheat. • This fungus is believed to have caused the mycotoxicosis epidemic in Russian between 1942 and 1948, and also earlier in 1913 and 1932. The region that was most affected by this epidemic was Russia’s Orenburg region near the Caspian Sea. Poisoning by ergot Cleviceps purpurea alkaloids (ergotism) • • Ergot spreads by wind, contaminates the soil and grain, fungal spores growing in a flower bed of grains, forming the body of sclerotia, where ergot alkaloids are produced. Ergot alkaloids: • • • affect the peripheral nervous system (limbs convulsions ), cause a hallucinogenic effect, cause dry gangrene of the extremities with the exclusion of the joints at chronic consumption of contaminated bread. • Factors: prolonged drought, the contamination of sowing soil by spores of ergot, non-application of pesticides and presowing treatment of seeds, lack of sanitary expertise of grain. • • MPC ergotoxines in grain=0,05 mg/kg Ergot sclerotia by mass: MPC in grain = 0,10,2% and MPC in flour = 0,05%. Hepatic veno-occlusive disease (VOD) in connection with the consumption of bread, the baked from grains mixed with weed seeds, such as heliotrope. • VOD occur after ingestion of certain plant alkaloids such as pyrrolizidine alkaloids. • Features of VOD include weight gain, tender hepatomegaly, ascites, increased bilirubin. It often is associated with renal failure. • VOD is fatal in over 30% of cases. • Poisoning outbreaks: • Senecio poisoning: South Africa, 1920. • Gurlan-disease: Western Afghanistan (province Herat), 1974-2008. • “Camel's belly»: Uzbekistan, 1931 and 1945. • Budd-Chiari-like syndrome: Central India, 19721973. Sanitary expertise of bread • I step is organoleptic expertise: • - surface of wheat or rye-bread: smooth, without large cracks and defects; • - collor: uniform, shining; • - form of bread stick: right, free of defects; • - crump: without inclusions, uniform good porosity, not adhesive, not humid; • - consistence: elastic, fast restoring form of bread; • - taste: pleasant, conforming to the kind of bread, without extraneous smack. I stage: bread humidity (H). • • • • • • Weigh 5 grams of bread-crumb in a metal box (a), Grind it into small particles. Place it into a thermostat (T=1300) for 40 min. Take the box out, immediately cover it, get it cold to room temperature. Weigh the box again (b). Account the bread humidity by the formula: ( a b) H (%) 100 5 • Compare with the normative: standard humidity of rye bread is ≤51%, wheaten bread - ≤47%, mixed bread - ≤50%. • Exceeding of the standard humidity creates conditions for bread spoiling (growing moldy and bacteria), deteriorates organoleptic properties, and degrades bread assimilability. • II stage: bread porosity (P). • Cut out a piece of bread-crumb using round knife, allowing to cut out a piece of constant volume 27 cm3 (V). Weigh the bread-crumb piece (W). • Account the bread porosity by the formula: W (V ) D P(%) [ ] 100 • V • D – density of bread-crumb mass without pores. D is estimated from table: Compare with normative: standard porosity of rye bread is ≥ 45%, wheaten bread - ≥50%. Insufficiency of the standard porosity deteriorates organoleptic properties, and brings down bread assimilability. Bread kind Rye or mixed Rue boiled Wheaten (2-nd sort) Wheaten (1-st sort) Density (g/cm3) 1.21 1.27 1.26 1.31 III stage: bread acidity (basic test of bread quality) • Acidity of bread (expressed in grades) is equal to volume of 1N solution of NaOH, spend on acids neutralization (in main acetic and lactic acids) of 100 g of bread. • Weigh 25 g of bread, reduce to fine particles, and place it into a flat-bottom flask (250 ml). • Add 50 ml of distilled water, and grind and shake it, trying to make homogeneous mixture. • Add 150 ml of distilled water, mix it again. • Decant liquid and filtrate it. • Place 50 ml of the filtrate into a flat-bottom flask (100 ml). • Add 2-3 drops of 1% phenolphthalein. • Titrate it by 0.1 N solution sodium hydroxide till stable pink coloring. Fix volume of titrant (ml). • Calculate acidity by the formula: A( Terner ) 1.6 V 0 • Compart with normative: standard acidity of rye bread is 120T, wheaten bread – 30T. • Give sanitation of the quality of bread studied, based on the studied properties. If some of them do not meet hygienic requirements, describe the risks to health. Hygienic evaluation of canned and concentrates. Preservation methods. • The basic directions of scientific development of conserving methods: – increasing in foodstuff storage period; – increasing of food value; – improvement of food properties including the taste, smell and appearance. • The reasons of food damage are – microorganism`s vital activity, – undesirable activity of the food enzymes, – oxidation of food components by air oxygen. • The purposes of conservation are destruction of microorganisms and undesirable activity of the enzymes and keeping from air. Physical methods of food conservation • Sterilization (100-140С, bactericidal effect) • Frying (bactericidal effect, but absence of guarantee of reaching temperature of sterilization inside the fried product (meat, fish) • Deep-frying (toxic substances formation from fat - alkyl-, alkoxy-, peroxyradicals, cancerogenic benzpyrene) • Boiling (bacteriostabilizing effect, decreasing of alimentary value of milk) • Pasteurization (60-70С during 15-30 minutes), fractional pasteurization (bacterial and fungi spores are not damaged) • Freezing (At violation of the rules - rapid freezing - defrosting slow - food cell destruction, loss of food juices - reduced nutritional value) → modern freezing: – in freezing chambers with a coolant (freon or ammonia; – in a special brick quick-freezing unit occurs 2-3 hours at less 5 cm thick layer of food (dough, pizza, bread); – in “boiling layer” allows to frost a food (berry, cut fruits and vegetables, small seafood) in some minutes. Coolants are streams of liquid nitrogen (-195С), dioxide of nitrogen or freon. – Hermetic packing of frozen fruit and vegetables allows keeping of vitamin C and humidity owing to exclude contact of the product to air oxygen and evaporation of water. – The storage period is a 1 year at -18С for vegetables and -12С for fruit. • Dehydration ways: • 1). Natural (dry-curing in a shadow and drying on the sun); • 2). Artificial (drying in furnaces, sublimation drying (lyophilization) or freeze-drying). Sterilization by ultrasound • UV - radiation sterilizes a surface of a firm product (for example, cheese) or transparent liquids in bottles from UV-glasses • Radioactive γ-radiation is used for sterilization of meat, for example, pork from Trichinella spiralis (before using technical or destruction) Biological methods • for storing apples, cabbage, cucumbers, tomatoes in open containers (barrels) in a cool place (cellar). • Fermentation of fruit and vegetable sugars by lactic bacteria leads to formation of a dairy acid at pickling and wetting: the dairy acid gradually cumulates and at concentration 0,7 % possesses preserving action. • Fermentation way includes addition of common salt and allows to storage at 0 +40C. • Russian national tradition involves cooking by this method of pickled apples, pickled cucumbers and tomatoes, sauerkraut for consumption during a long winter. Chemical methods • Pickling (NaCl) - plazmolisis of bac.cells (Meat - 10-12 %, fishes - 14 %, tomato paste - 10 %). • Smoking of meat, sausages and fishes can be carried out by the cold way (240C) or the hot way (raised T). At smoking septic action is rendered with products of wood high temperature sublimation (phenols, formaldehydes, creosote, benz(a)pyrene and an acetic acid). • Sugar (60-65%) – plazmolisis of bac.cells • Organic acids • Semi-preservation (The agents are salt, acetic acid and spices (essential oil)) for fish: • Sulfitation (sodium sulfate, sulfite of sodium NaHSO3 (E221E227)). • Sulphurous anhydride represents a water solution of sulphurous gas SO2, which suppresses fungi, yeast and aerobic bacteria growth. Unaerobic microflora is to a lesser degree subject to action of sulphurous anhydride. Besides sulphurous anhydride and solutions of sulfites possess regenerative properties, inhibit of dehydrogenazes, protecting a potato, vegetables and fruit. • Sulphurous anhydride is poisonous for human organism, but easily disappears at heating and long contact to air. The level of comprehensible daily consumption is 0,7 mg/kg of body weight. One glass of sulfited juice gives 1,2 mg of sulphurous anhydride, 200 g of zephyr or a fruit candy - 4 mg, 200 ml of wine - 30 mg/l (=MPC). The daily use of sulfited products can lead to excess of an admissible daily doze. Sulphites in an organism turn to sulfates which are well deduced with urine and excrements. Code Е-200 Е-209 Е-210 Е-213 Е-214 Е-215 Е-216 Е-217 Е-218 Е-219 Е-220 Е-221 Е-225 Е-226 Е-227 Е-228 Е-230 Е-231 Е-232 Е-233 Е-234 Е-235 Е-236 Е-237 Е-238 Е-239 Е-240 Е-241** Е-249 Е-252** Е-261 Е-262 Е-263 Е-264 Е-281 Е-282 Е-283 Е-284 Е-285 Е-296 Е-297 Name Sorbic acid Heptil ether of para-hydrobenzoic acid Benzoic acid Benzoate calcium Ethyl ether of para-hydrobenzoic acid Sodium salt of ethyl ether of para-hydrobenzoic acid Propyl ether of para-hydrobenzoic acid Sodium salt of propyl ether of para-hydrobenzoic acid Methyl ether of para-hydrobenzoic acid Sodium salt of methyl ether of para-hydrobenzoic acid Sulfur dioxide Sodium sulfite Potassium sulfite Calcium sulfite Calcium hydrosulfite Potassium hydrosulfite (potassium bisulfite) Biphenyl Orto-phenyl phenol Sodium orto- phenyl phenol Тиабендазол thiabendazol Nizine Natamicyne Formic acid Sodium formiate Calcium formiate Hexamethylenamine (urotropine) Formaldehyde Guaiacum gum Potassium nitrite Potassium nitrate Potassium acetate Sodium diacetate Calcium acetate Ammonium acetate Sodium propionate Calcium propionate Potassium propionate Boric acid Sodium tertaborat (бура) Malonic acid Fumaric acid The note Can cause skin reactions Can cause an attack of an asthma It is forbidden in a number of the countries It is forbidden in a number of the countries It is forbidden in a number of the countries Skin allergic reactions are possible It is forbidden in a number of the countries To people with diseases of kidneys to apply with care It is forbidden in a number of the countries It is forbidden in a number of the countries It is forbidden in a number of the countries It is forbidden in a number of the countries It is forbidden in a number of the countries Can cause allergic reactions, a nausea, a diarrhea It is forbidden in a number of the countries It is forbidden in a number of the countries It is forbidden in a number of the countries It is forbidden in a number of the countries It is forbidden in Russia and in a number of other countries It is forbidden to use in a children's feed. Probable carcinogen. In many countries on its use restrictions are imposed It should be avoided to people with diseases of kidneys Can make a sick Can cause a headache, a migraine Can cause a headache, a migraine Can cause a headache, a migraine Toxic with high cumulating ability It is not recommended to babies and small children Sanitary estimation of canned food • A standard set of research involves – humidity (conditions for growth of molds) – acidity (acidity > 0.4-0.6% in re-count to malic acid is a sign of the turned sour food), – content of NaCl (normal content in canned food – 1.5-3%, in preserves – 9-13%), – content of copper salts (MPC = 5 - 60 mg/kg for different kinds of canned food), – content of zinc salts (MPC = 200 mg/kg), – content of lead (is forbidden). Revealing discrepancy of various information blocks put on various places of the pack • 1). Marks of tin should be stamped or put by an indelible paint and consist of 3-6 signs. • 1-st number: date of the manufacturing – a number (2 signs), a month (2 numbers or a letter, excepting the letter "З"), a year (2 or 1 last figure). • 2-nd number: assortment number (from 1 up to 3 numbers or letters, M - meat-and-milk (мясо, молоко), Р - fishcanning (рыба), K - fruit-vegetable (фрукты, овощи)); number of a factory (from 1 up to 3 numbers or letters). • 3-rd number: the manufacturing plant (1 sign). Examination of packaging for its damages • Tin corrosion (blue-brown film on internal surface), food acids tin damaging - marblization or sulphidic corrosion (for protein canned food). • True bombazh can as sign of reproduction of bacteria Clostridium botulinum (differ false bombazh – overflow). • Violation of integrity of an internal canning varnishes (oxidized linen, dehydrate castor, condensed tung oils). • Conditions of storage of canned food: 0-200C and humidity ≤75%; of jam - 15-200C. Bar-code EAN (European Article Numbering) Rules for checking the electronic bar-code EAN-13 on falsification 1. Sum the numbers, standing on the even places from left to right (2, 4, 6, 8, 10,12). 2. Multiply the sum by 3. 3. Sum the numbers, standing on the odd places from left to right (1, 3, 5, 7, 9, 11). 4. Sum the figures obtained at points 2 and 3. 5. If you receive a two-digit number drop tens (i.e. the first of two): 6. Calculate the difference between the number 10 and received at point 5 number (example: 10-3=7). The resulting figure should be identical to the target figure. If the numbers are not identical, the product should be recognized as a falsified (unsuitable).