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Chapter 19: Two-Factor Studies with Equal Sample Size Lecture 12 March 29, 2007 Psych 791 Slide 1 of 33 Seen Previously ■ Tuesday we learned about all that great terminology and all those wonderful Greek letters. ■ As a review: Overview ● Previous Class ● Today’s Class ● Today’s Example Cell Means Model ◆ αi is the effect of Factor A. ◆ βj is the effect of Factor B. ◆ (αβ)ij is the interaction effect of Factors A and B. Factor Effects Model Fitting The Model Testing Effects Wrapping Up Slide 2 of 33 Today’s Class ■ Overview Hopefully, by the end of this class, you will know: ◆ The formula for a 2-way ANOVA using the Cell Means model. ◆ The formula for a 2-way ANOVA using the Factor Effect model. ◆ The relationship between the two models. ◆ How to formulate both the model and design matrix for a 2-way ANOVA using the Factor Effects model. ◆ And, most important, how to test for both main effects and interaction effects. ● Previous Class ● Today’s Class ● Today’s Example Cell Means Model Factor Effects Model Fitting The Model Testing Effects Wrapping Up Slide 3 of 33 Today’s Example ■ Overview ● Previous Class ● Today’s Class ● Today’s Example Cell Means Model Factor Effects Model Fitting The Model Testing Effects Wrapping Up From Street and Carroll (1972): In testing food products for palatability, General Foods employed a 7-point scale from -3 (terrible to +3 (excellent) with 0 representing "average". Their standard method for testing palatability was to conduct a taste test with 50 persons - 25 men and 25 women. The experiment reported here involved the effects on palatability of a course versus fine screen and of a low versus high concentration of a liquid component. Four groups of 50 consumers each were recruited from local churches and club groups. Persons were assigned randomly to the four treatment groups as they were recruited. The experiment was replicated four times, so that there were 16 groups of 50 consumers each in the entire experiment Slide 4 of 33 Today’s Example Data Score Liquid Screen 35 0 0 39 0 0 77 0 0 ● Today’s Example 16 0 0 Cell Means Model 104 1 0 Factor Effects Model 129 1 0 Fitting The Model 97 1 0 Testing Effects 84 1 0 Wrapping Up 24 0 1 21 0 1 39 0 1 60 0 1 65 1 1 94 1 1 86 1 1 64 1 1 Overview ● Previous Class ● Today’s Class Slide 5 of 33 Cell Means Model Slide 6 of 33 Cell Means Model ■ Our ANOVA model can now be stated as follows: Overview Yijk = µij + εijk Cell Means Model ● Matrix Version ● Additional Comments ■ where: ■ µij are the parameters: treatment means. ■ εijk are independent N (0, σ 2 ). ■ i = 1,...,a; ■ j = 1,...,b; ■ k = 1,...,n. Factor Effects Model Fitting The Model Testing Effects Wrapping Up Slide 7 of 33 Representation in Matrix Formula ■ Using our example’s 2X2 design: Overview Cell Means Model ● Matrix Version ● Additional Comments Factor Effects Model Fitting The Model Testing Effects Wrapping Up Slide 8 of 33 Additional Comments ■ While this representation is easy to do, it isn’t particularly interesting. ■ All you are recovering is the treatment level means. ■ Testing main effects would require you to perform contrasts on the cell means. ■ Seems like it is back tracking. ■ We want to parameterize the model is such a way that it is easy to test to main effects. ■ Oh, and we don’t want to abandon those nice little αs and βs that we talked about on Tuesday. Overview Cell Means Model ● Matrix Version ● Additional Comments Factor Effects Model Fitting The Model Testing Effects Wrapping Up Slide 9 of 33 Factor Effects Model Slide 10 of 33 Cell Means Model Alteration ■ We are going to take the cell means model, and put it in a format that is more intuitive for our analysis. ■ To do this, we will replace each treatment mean: Overview Cell Means Model Factor Effects Model ● Matrix Formula µij Fitting The Model Testing Effects Wrapping Up ■ with this: µij = µ·· + αi + βj + (αβ)ij ■ This will make the model... Slide 11 of 33 Factor Effects Model ■ With our substitution: Overview Yijk = µ·· + αi + βj + (αβ)ij + εijk Cell Means Model Factor Effects Model ● Matrix Formula ■ µ·· is a constant (the grand mean). ■ εijk are independent N (0, σ 2 ). P αi constant with αi = 0. P βj constant with βj = 0. P P i (αβ)ij = 0 and j (αβ)ij = 0. Fitting The Model Testing Effects Wrapping Up ■ ■ ■ ■ P P i j (αβ)ij = 0. Slide 12 of 33 Matrix Formula ■ Overview Cell Means Model Factor Effects Model ● Matrix Formula Fitting The Model Testing Effects Wrapping Up Slide 13 of 33 Fitting The Model Slide 14 of 33 Fitting the Model ■ Basically take every formula that we talked about up to now, and replace the µ’s with Y. ■ In condensed form, the estimated model is: Overview Cell Means Model Factor Effects Model Fitting The Model Ybijk = Ȳ··· + (Ȳi·· − Ȳ··· ) + (Ȳ·j· − Ȳ··· ) + (Ȳij· − Ȳi·· − Ȳ·j· + Ȳ··· ) ● Sum of Squares ● Partitions ● Decomposition ● Example ● Degrees of Freedom ● Partitioning DF ■ With residuals being: ● Mean Squares Testing Effects Wrapping Up eijk = Yijk − Ȳij· Slide 15 of 33 In SAS... Overview Cell Means Model Factor Effects Model proc glm data=palate; class liquid screen; model score=liquid|screen/solution; lsmeans liquid|screen; run; Fitting The Model ● Sum of Squares ● Partitions ● Decomposition ● Example ● Degrees of Freedom ● Partitioning DF ● Mean Squares Testing Effects Wrapping Up Slide 16 of 33 Sum of Squares Overview ■ We are doing ANOVA, so will be analyzing some variances. ■ We are going to take our total sum of squares and do some partitioning: Cell Means Model Factor Effects Model XXX Fitting The Model i ● Sum of Squares j k 2 (Yijk − Ȳ··· ) = n XX XXX 2 2 (Ȳij· − Ȳ··· ) + (Yijk − Ȳij· ) i j i j k ● Partitions ● Decomposition ● Example ● Degrees of Freedom ● Partitioning DF ■ The shortened form is this: ● Mean Squares Testing Effects SST O = SST R + SSE Wrapping Up Slide 17 of 33 Partitioning of Treatment SS ■ Since we have more than one effect, we need to partition our sum of squares. ■ Remember when we did all the great partitioning in multiple regression, well, all this partitioning will be done for us. ■ Here is the long formula: Overview Cell Means Model Factor Effects Model Fitting The Model ● Sum of Squares ● Partitions ● Decomposition ● Example n ● Degrees of Freedom ● Partitioning DF XX X X XX 2 2 2 2 (Ȳij· −Ȳ··· ) = nb (Ȳi·· −Ȳ··· ) +na (Ȳ·j· −Ȳ··· ) +n (Ȳij· −Ȳi·· −Ȳ·j· +Ȳ··· ) i j i j i j ● Mean Squares Testing Effects Wrapping Up ■ And the short formula is this: SST R = SSA + SSB + SSAB Slide 18 of 33 Decomposition ■ So now, I will spare you the long formula and get right to the short formula. ■ Our total error variance is going to be partitioned in this way: Overview Cell Means Model Factor Effects Model SST O = SSA + SSB + SSAB + SSE Fitting The Model ● Sum of Squares ● Partitions ● Decomposition ● Example ● Degrees of Freedom ■ Just like in regression, we want to see how much variance is attributed to which effect. ■ The larger the variability (or larger the partition), the more of an effect there is. ● Partitioning DF ● Mean Squares Testing Effects Wrapping Up Slide 19 of 33 SAS Example The GLM Procedure Dependent Variable: score Source Model Error Corrected Total DF 3 12 15 R-Square 0.720813 Source liquid screen liquid*screen Sum of Squares 12053.25000 4668.50000 16721.75000 Coeff Var 30.52091 DF 1 1 1 Mean Square 4017.75000 389.04167 Root MSE 19.72414 Type III SS 10609.00000 1024.00000 420.25000 F Value 10.33 Pr > F 0.0012 F Value 27.27 2.63 1.08 Pr > F 0.0002 0.1307 0.3191 score Mean 64.62500 Mean Square 10609.00000 1024.00000 420.25000 Slide 20 of 33 Degrees of Freedom ■ As you remember from all ANOVA tables, there are degrees of freedom associated with each effect. ■ We need to break up our total degrees of freedom we have in our data (total subjects - 1) into it’s particular parts. ■ You will notice that the title of the chapter is "Two-Factor Studies with Equal Sample Sizes". ■ This idea of equal sample sizes refers to the number of subjects in each treatment group. ■ There is an equal number in each treatment group. ■ We are going to refer to the number of subjects in each treatment group as n. ■ Therefore, our total sample size is nT = a × b × n. Slide 21 of 33 Partitioning DF Overview ■ Our partitioning of our Degrees of Freedom will be as follows: ■ Factor A: a − 1 ■ Factor B: b − 1 ■ AB Int: (a − 1)(b − 1) ■ error: ab(n − 1) ■ Total: abn − 1 Cell Means Model Factor Effects Model Fitting The Model ● Sum of Squares ● Partitions ● Decomposition ● Example ● Degrees of Freedom ● Partitioning DF ● Mean Squares Testing Effects Wrapping Up Slide 22 of 33 Mean Squares Overview ■ Do you remember how to get a mean square? ■ Take the Sum of Squares and Divide by your Degrees of Freedom: Cell Means Model Factor Effects Model SS/df Fitting The Model ● Sum of Squares ● Partitions ● Decomposition ● Example ● Degrees of Freedom ■ So: ● Partitioning DF ● Mean Squares Testing Effects Wrapping Up M SA = SSA/(a − 1) M SB = SSB/(b − 1) M SAB = SSAB/(a − 1)(b − 1) M SE = SSE/ab(n − 1) Slide 23 of 33 Testing Effects Slide 24 of 33 Testing for Effects Overview ■ Hypothesis tests should all look familiar. ■ To test effects, you are going to basically test for a significant amount of variance in the effect. ■ How do you do this? ■ Going to be an F-test. Cell Means Model Factor Effects Model Fitting The Model Testing Effects ● Testing for Main Effect of A ● Testing for Main Effect of B ● Testing for Interaction Effect of A × B Wrapping Up Slide 25 of 33 Testing for Main Effect of A ■ Null Hypothesis: Overview H0 : α1 = α2 = . . . = αa = 0 Cell Means Model Factor Effects Model Ha : not all αi = 0 Fitting The Model Testing Effects ● Testing for Main Effect of A ● Testing for Main Effect of B ● Testing for Interaction Effect of A × B ■ or alternatively: H0 : µ1· = µ2· = . . . = µa· Wrapping Up Ha : not all µi· equal ■ To test: M SA F (a − 1, ab(n − 1)) = M SE Slide 26 of 33 SAS Example The GLM Procedure Dependent Variable: score Source Model Error Corrected Total DF 3 12 15 R-Square 0.720813 Source liquid screen liquid*screen Sum of Squares 12053.25000 4668.50000 16721.75000 Coeff Var 30.52091 DF 1 1 1 Mean Square 4017.75000 389.04167 Root MSE 19.72414 Type III SS 10609.00000 1024.00000 420.25000 F Value 10.33 Pr > F 0.0012 F Value 27.27 2.63 1.08 Pr > F 0.0002 0.1307 0.3191 score Mean 64.62500 Mean Square 10609.00000 1024.00000 420.25000 Slide 27 of 33 Testing for Main Effect of B ■ Null Hypothesis: Overview H0 : β1 = β2 = . . . = βb = 0 Cell Means Model Factor Effects Model Ha : not all βj = 0 Fitting The Model Testing Effects ● Testing for Main Effect of A ● Testing for Main Effect of B ● Testing for Interaction Effect of A × B ■ or alternatively: H0 : µ·1 = µ·2 = . . . = µ·b Wrapping Up Ha : not all µ·j equal ■ To test: M SB F (b − 1, ab(n − 1)) = M SE Slide 28 of 33 SAS Example The GLM Procedure Dependent Variable: score Source Model Error Corrected Total DF 3 12 15 R-Square 0.720813 Source liquid screen liquid*screen Sum of Squares 12053.25000 4668.50000 16721.75000 Coeff Var 30.52091 DF 1 1 1 Mean Square 4017.75000 389.04167 Root MSE 19.72414 Type III SS 10609.00000 1024.00000 420.25000 F Value 10.33 Pr > F 0.0012 F Value 27.27 2.63 1.08 Pr > F 0.0002 0.1307 0.3191 score Mean 64.62500 Mean Square 10609.00000 1024.00000 420.25000 Slide 29 of 33 Testing for Interaction Effect of A × B ■ Null Hypothesis: Overview H0 : all (αβ)ij = 0 Cell Means Model Factor Effects Model Ha : not all (αβ)ij = 0 Fitting The Model Testing Effects ● Testing for Main Effect of A ● Testing for Main Effect of B ● Testing for Interaction Effect of A × B ■ or alternatively: H0 : all µij equal Wrapping Up Ha : not all µij equal ■ To test: F ((a − 1)(b − 1), ab(n − 1)) = M SAB M SE Slide 30 of 33 SAS Example The GLM Procedure Dependent Variable: score Source Model Error Corrected Total DF 3 12 15 R-Square 0.720813 Source liquid screen liquid*screen Sum of Squares 12053.25000 4668.50000 16721.75000 Coeff Var 30.52091 DF 1 1 1 Mean Square 4017.75000 389.04167 Root MSE 19.72414 Type III SS 10609.00000 1024.00000 420.25000 F Value 10.33 Pr > F 0.0012 F Value 27.27 2.63 1.08 Pr > F 0.0002 0.1307 0.3191 score Mean 64.62500 Mean Square 10609.00000 1024.00000 420.25000 Slide 31 of 33 Final Thought ■ We saw today how interaction terms are parameterized. ■ We also so how such terms are placed in the GLM context with matrix forms. ■ Interactions are straightforward extensions of the modeling framework you already know (see your notes on higher order models from last semester). ■ Interpretation of interactions is less straightforward. Overview Cell Means Model Factor Effects Model Fitting The Model Testing Effects Wrapping Up ● Final Thought ● Next Class Slide 32 of 33 Next Time ■ On Tuesday we will finish up the chapter by talking about what to do when effects are significant ■ Then, we will also have a data analysis example to reiterate everything we have talked about Overview Cell Means Model Factor Effects Model Fitting The Model Testing Effects Wrapping Up ● Final Thought ● Next Class Slide 33 of 33