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Chapter 27 Reproduction and Embryonic Development PowerPoint Lectures for Biology: Concepts and Connections, Fifth Edition – Campbell, Reece, Taylor, and Simon Lectures by Chris Romero Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Baby Bonanza • The increased use of fertility drugs has caused an increase in the number of multiple births in the United States • Infertility affects about one in seven couples in the United States • Fertility drugs are an example of technology affecting the normal reproductive cycle Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings ASEXUAL AND SEXUAL REPRODUCTION 27.1 Sexual and asexual reproduction are both common among animals • In asexual reproduction, one parent produces genetically identical offspring – Budding, fission, fragmentation/ regeneration – Allows isolated animals to reproduce without having to find mates – Produces offspring quickly – Diminishes genetic diversity of offspring Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings • Sexual reproduction involves the fusion of gametes from two parents – Two haploid gametes unite to produce a diploid zygote – Male sperm is small, flagellated – Female ovum is larger and not selfpropelled – Increases genetic variability among offspring – May enhance reproductive success in changing environments Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings • Many animals can reproduce both sexually and asexually – Rotifer • Asexual in good environmental conditions • Sexual in more stressful conditions – Hermaphrodites • Each individual has both female and male reproductive systems • Most mate with another member of their species Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings LE 27-01c “Head” Intestine Ovary Eggs Video: Rotifer Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings • External fertilization is common in aquatic animals – Parents release their gametes into the environment, where fertilization occurs • Internal fertilization occurs in nearly all terrestrial animals – Sperm are deposited in or close to the female reproductive tract – Gametes unite within the tract – Usually requires copulation Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings LE 27-01e Eggs HUMAN REPRODUCTION 27.2 Reproductive anatomy of the human female • Both sexes in humans – Have a set of gonads where gametes are produced – Have ducts for delivery of the gametes and structures for copulation Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings • Ovaries – Produce egg cells (ova) – Follicles protect and nourish the eggs and produce estrogen • Ovulation • One egg released every 28 days, starting at puberty – Remaining follicular tissue temporarily develops into corpus luteum • Secretes progesterone Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings • Oviducts (fallopian tubes) – Convey eggs to the uterus – Location of fertilization • Uterus (womb) – Thick wall and endometrium richly supplied with blood vessels – Actual site of pregnancy • Embryo: from first division of zygote until body structures begin to appear • Fetus: from about ninth week until birth Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings – In ectopic pregnancy, embryo implants somewhere other than uterus • Cervix: narrow neck of uterus • Vagina: Muscular opening from uterus – Receives penis during intercourse – Forms the birth canal • Other structures of the female reproductive system – Labia minora and majora, clitoris, Bartholin's gland Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings LE 27-02a Ovaries Oviduct Follicles Corpus luteum Wall of uterus Uterus Endometrium (lining of uterus) Cervix (“neck” of uterus) Vagina LE 27-02c Oviduct Ovary Uterus Rectum (digestive system) Urinary bladder (excretory system) Pubic bone Cervix Urethra (excretory system) Vagina Shaft Glans Bartholin’s gland Anus (digestive system) Clitoris Prepuce Labia minora Vaginal opening Labia majora LE 27-02b Egg cell Ovary Animation: Female Reproductive Anatomy Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings 27.3 Reproductive anatomy of the human male • Testes, housed outside the body in the scrotum, produce sperm • Pathway of sperm from testis to outside – Epididymis – Vas deferens – Ejaculatory duct – Ejaculation through urethra in penis • Connection between reproductive and excretory systems Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings • Several glands contribute to the formation of fluid that nourishes and protects sperm – Seminal vesicles – Prostate gland – Bulbourethral glands – Semen combines sperm and glandular secretions Animation: Male Reproductive Anatomy Animation: Male Hormones Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings LE 27-03a Rectum (digestive system) Seminal vesicle Urinary bladder (excretory system) Vas deferens Pubic bone Ejaculatory duct Erectile tissue of penis Prostate gland Bulbourethral gland Vas deferens Epididymis Testis Scrotum Urethra (excretory system) Glans of penis Prepuce Penis LE 27-03b Urinary bladder (excretory system) Seminal vesicle (behind bladder) Prostate gland Bulbourethral gland Erectile tissue of penis Vas deferens Urethra Scrotum Epididymis Testis Glans of penis • The process of ejaculation involves coordinated contractions and expulsion of fluids • Hormones control sperm production by the testes through a negative-feedback system Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings LE 27-03c Sphincter contracts Contractions of vas deferens Urinary bladder Urethra region here expands and fills with semen Contractions of seminal vesicle Contractions of prostate gland First stage Sphincter contracts Sphincter remains contracted Contractions of epididymis Semen expelled Contractions of muscles around base of penis Sphincter relaxes Second stage Contractions of urethra LE 27-03d Stimuli from other areas in the brain Hypothalamus Anterior pituitary FSH LH Androgen production Testis Sperm production Negative feedback Releasing hormone 27.4 The formation of sperm and ova requires meiosis • Gametogenesis: the formation of diploid gametes from haploid sperm and ova during meiosis • Spermatogenesis: the formation of sperm cells – Diploid cells made continuously in seminiferous tubules of testes – Differentiated primary spermatocytes – Haploid secondary spermatocytes – Haploid sperm Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings LE 27-04a Epididymis Testis Scrotum Penis Diploid cell 2n Differentiation and onset of Meiosis I 2n Primary spermatocyte (in prophase of Meiosis I Meiosis I completed Cross section of seminiferous tubule Secondary spermatocyte (haploid; double chromatids) n n Testis Seminiferous tubule Meiosis II n n n n Developing sperm cells (haploid; single chromatids) Differentiation n n n n Sperm cells (haploid) Center of seminiferous tubule • Oogenesis occurs mostly in the ovaries – Before birth, a diploid cell in each developing follicle begins meiosis – At birth, each follicle contains a dormant diploid primary oocyte – After puberty, one primary oocyte is released each month • Continues meiosis • Unequal division of cytoplasm forms a single secondary oocyte • If fertilized, oocyte completes meiosis and becomes a haploid ovum Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings LE 27-04b Diploid cell 2n In embryo Differentiation and onset of Meiosis I Primary oocyte 2n (arrested in prophase of Meiosis I) Present at birth Completion of Meiosis I and onset of Meiosis II Secondary oocyte (arrested at metaphase of Meiosis II; released from ovary) n n First polar body Entry of sperm triggers completion of Meiosis II Ovum (haploid) n n Second polar body • The development of an ovarian follicle involves many different processes • Comparison of oogenesis and spermatogenesis – Both produce haploid gametes – Only one ovum results from each diploid cell that undergoes meiosis – Cells from which gametes develop are thought not to divide throughout life in the female – Oogenesis has long resting periods; spermatogenesis is uninterrupted Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings LE 27-04c Degenerating corpus luteum Start: Primary oocyte within follicle Corpus luteum Growing follicles Mature follicle Secondary oocyte Ovary Ovulation Ruptured follicle 27.5 Hormones synchronize cyclic changes in the ovary and uterus • The reproductive cycle in females involves an integrated process between the ovaries and the uterus – Ovarian cycle: produces the oocyte – Menstrual cycle: involves the monthly changes in the uterus – Hormonal messages synchronize the two cycles through intricate feedback systems Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings • Events in the menstrual cycle are coordinated with the ovarian cycle – Events synchronized by five hormones – Menstrual cycle prepares uterus for implantation of embryo – Menstruation lasts 3-5 days, corresponding to pre-ovulatory phase of ovarian cycle – Continues through time of ovulation (20-25 days) – If no embryo is implanted, menstruation begins again Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings • Hormonal events leading to ovulation – Hypothalamus signals the anterior pituitary to secrete FSH and LH – FSH stimulates growth of a follicle, with little estrogen secreted and negative feedback control of pituitary – Increased secretion of estrogen exerts positive feedback on hypothalamus – Pituitary secretes burst of FSH and LH levels – LH, FSH, and estrogen peak Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings • Hormonal events at ovulation and after – LH stimulates completion of meiosis and rupture of follicle – Ovulation occurs – Follicle becomes the corpus luteum – Corpus luteum secretes estrogen and progesterone – High levels of estrogen and progesterone exert negative feedback on hypothalamus and pituitary Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings – FSH and LH levels drop – Hypothalamus stimulates pituitary to secrete more FSH and LH, and a new cycle begins • Control of the menstrual cycle – When levels of estrogen and progesterone drop, endometrium begins to slough off – Menstrual bleeding begins on day 1 of a new cycle Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings LE 27-05 Control by hypothalamus Inhibited by combination of estrogen and progesterone Hypothalamus Stimulated by high levels Releasing hormone of estrogen Anterior pituitary FSH LH Pituitary hormones in blood LH peak triggers ovulation and corpus luteum formation LH FSH LH FSH Ovarian cycle Growing follicle Mature follicle Ovulation Pre-ovulatory phase Estrogen Corpus luteum Degenerating corpus luteum Post-ovulatory phase Progesterone and estrogen Ovarian hormones in blood Estrogen Progesterone Estrogen Progesterone and estrogen Animation: Ovulation Animation: Post Ovulation Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings 27.6 The human sexual response occurs in four phases • Excitement phase: prepares the sexual organs for coitus • Plateau phase: breathing and heart rate increase • Orgasm: rhythmic contractions, pleasure for both partners, ejaculation • Resolution phase: completes the cycle and reverses the previous responses Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings CONNECTION 27.7 Sexual activity can transmit disease • Sexually transmitted diseases (STDs) are contagious diseases spread by sexual contact • Viral diseases are not curable but can be controlled by medication • Many STDs can cause long-term problems or even death if untreated • STDs are most prevalent among teenagers and young adults Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings CONNECTION 27.8 Contraception can prevent unwanted pregnancy • Contraception is the deliberate prevention of pregnancy • Contraception must be used correctly to prevent failure • "Safe sex" provided by condoms can prevent both unwanted pregnancy and sexually transmitted disease Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings • Common contraceptive methods – Abstinence – Tubal ligation or vasectomy – Rhythm method (natural family planning) – Withdrawal – Barrier methods: condom, diaphragm • Most effective if used with spermicide – Oral contraceptives – Morning after pills Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings LE 27-08 Skin patch Condom Diaphragm Spermicide Birth control pills PRINCIPLES OF EMBRYONIC DEVELOPMENT 27.9 Fertilization results in a zygote and triggers embryonic development • Embryonic development begins with fertilization – Many sperm reach an egg, but only one fertilizes it – Sperm and egg unite to form a diploid zygote Video: Hydra Releasing Sperm Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings • The properties of sperm cells – Streamlined shape an adaptation for swimming – Thick head contains haploid nucleus – Acrosome at tip of head contains enzymes that help sperm penetrate the egg – High-energy nutrients absorbed from semen provide energy for propulsion and penetration Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings LE 27-09b Plasma membrane Middle piece Neck Head Tail Mitochondrion (spiral shape) Nucleus Acrosome • The process of fertilization 1. Sperm approaches egg 2. Sperm's acrosomal enzymes digest egg's jelly coat 3. Proteins on sperm head bind to egg receptors 4. Plasma membranes of sperm and egg fuse 5. Sperm nucleus enters egg cytoplasm Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings 6. Fertilization envelope forms, preventing other sperm from entering egg 7. Nuclei of sperm and egg fuse, producing diploid nucleus of zygote Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings LE 27-09c The sperm approaches the egg Sperm The sperm’s acrosomal enzymes digest the egg’s jelly coat Proteins on the sperm head bind to egg receptors The plasma membranes of sperm and egg fuse Sperm head Nucleus The sperm nucleus enters the egg cytoplasm Acrosome Plasma membrane Acrosomal enzymes Vitelline layer A fertilization envelope forms Receptor protein molecules Plasma membrane Sperm nucleus Cytoplasm Egg nucleus Jelly coat Egg cell The nuclei of sperm and egg fuse Zygote nucleus 27.10 Cleavage produces a ball of cells from the zygote • Cleavage is the first major stage of embryonic development • A rapid series of cell divisions produces a ball of cells from the zygote – The number of cells doubles with each cleavage division • Fluid-filled blastocoel forms in the center of the embryo • A blastula, a hollow ball of cells, is the product of cleavage Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings LE 27-10 Zygote 2 cells 4 cells 8 cells Blastocoel Many cells (solid ball) Blastula (hollow ball) Cross section of blastula Video: Sea Urchin Embryonic Development Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings • Cleavage makes two important contributions to early development – Creates a multicellular embryo from a single-celled zygote – Partitions the embryo into developmental regions Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings 27.11 Gastrulation produces a three-layered embryo • Gastrulation adds more cells to the embryo and sorts them into three layers – Ectoderm: forms outer skin of gastrula – Endoderm: forms embryonic digestive tract (archenteron) – Mesoderm: partly fills space between ectoderm and endoderm – These layers develop into all parts of the adult animal Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings • Mechanics of gastrulation 1. Blastula 2. Blastopore formation 3. Cell migration to form layers 4. Completion of gastrulation Video: Frog Embryo Development Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings LE 27-11 Animal pole Blastocoel Blastula Vegetal pole Gastrulation Blastopore forming Blastopore forming Blastocoel shrinking Archenteron Archenteron Ectoderm Mesoderm Endoderm Yolk plug Yolk plug Gastrula 27.12 Organs start to form after gastrulation • The three embryonic tissue layers differentiate into tissues and organs • Frog development in first few hours after gastrulation – Notochord in mesoderm • Will become core of the backbone – Hollow nerve cord in ectoderm • Neural plate forms neural tube, which will become the brain and spinal cord Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings LE 27-12a Neural Neural fold plate Notochord Ectoderm Mesoderm Endoderm Archenteron Neural folds LE 27-12b Neural fold Neural plate Outer layer of ectoderm Neural tube • Relative positions of neural tube, notochord, and archenteron preview the basic body plan • Changes about 12 hours later – Somites from mesoderm; will give rise to segmented structures such as vertebrae – Hollow coelom (body cavity) • By 5-8 days after beginning of development, all body tissues and organs would have emerged from cells of the three original layers Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings LE 27-12c Neural tube Notochord Somite Coelom Archenteron (digestive cavity) Somites Tail bud SEM 15 Eye 27.13 Changes in cell shape, cell migration, and programmed cell death give form to the developing animal • Changes in cell shape – Folds of ectoderm become the start of the brain and spinal cord • Migration of cells – Move to specific destinations – Join together, held by glycoproteins – Take on characteristics of a tissue Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings LE 27-13a Ectoderm • Programmed cell death, or apoptosis – Controlled by "suicide" genes – Essential for normal development and differentiation of body structures Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings LE 27-13b Apoptosis Dead cell engulfed and digested by adjacent cell 27.14 Embryonic induction initiates organ formation • All developmental processes depend on signals between cells • Induction: the mechanism by which one group of cells influences development of an adjacent group • A sequence of inductive signals leads to increasingly greater specialization of cells into organs Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings LE 27-14 Lens ectoderm Optic cup Cornea Future brain Lens Optic vesicle Optic stalk Future retina 27.15 Pattern formation organizes the animal body • Pattern formation is emergence of a body form with specialized parts in the right places • Positional signals determine which master control genes will be expressed • In vertebrates, pattern-forming zones provide positional information to other cells through chemical signals • A major goal of developmental research is to learn how information in DNA directs development of 3-D animal form Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings LE 27-15a Anterior Bird embryo Ventral Limb bud Limb bud develops Distal Dorsal Proximal Posterior Normal wing LE 27-15b Donor limb bud Graft of cells from patternforming zone Donor cells Host limb bud Graft Host limb bud develops Host patternforming zone Wing with duplication HUMAN DEVELOPMENT 27.16 The embryo and placenta take shape during the first month of pregnancy • Pregnancy, or gestation, is the carrying of developing young within the female reproductive tract – Gestation period varies considerably within animal species Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings • An overview of human developmental events – Fertilization in the oviduct – Blastocyst implanted in endometrium • Fluid-filled cavity • Cell mass that will form baby • Trophoblast – Secretes enzymes enabling implantation; forms part of placenta • Extraembryonic membranes Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings LE 27-16a Cleavage starts Fertilization of ovum Ovary Oviduct Secondary oocyte Ovulation Blastocyst (implanted) Endometrium Uterus LE 27-16b Endometrim Inner cell mass Cavity Trophoblast LE 27-16c Endometrium Future embryo Future yolk sac Blood vessel (maternal) Multiplying cells of trophoblast Trophoblast Actual size Uterine cavity • Roles of the extraembryonic membranes – Amnion has grown to enclose the embryo – Yolk sac produces embryo's first blood cells and germ cells – Allantois forms part of umbilical cord and part of urinary bladder – Chorion becomes embryo's part of placenta • HCG maintains production of estrogen and progesterone during early pregnancy Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings LE 27-16d Amniotic cavity Mesoderm cells Amnion Chorion Yolk sac LE 27-16e Chorion Amnion Allantois Yolk sac Chorionic villi Embryo: Ectoderm Mesoderm Endoderm • The placenta: composite organ consisting of chorionic villi closely associated with blood vessels of mother's endometrium – Chorionic villi • Absorb food and oxygen from the mother's blood – Chorionic blood vessels • Carry food, oxygen, protective antibodies, some viruses and toxins to fetus • Carry wastes back to mother's bloodstream Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings LE 27-16f Mother’s blood vessels Allantois Yolk sac Placenta Amniotic cavity Amnion Embryo Chorion Chorionic villi 27.17 Human development from conception to birth is divided into three trimesters • First trimester – All organs and appendages are built in essentially a human pattern – By 9 weeks, embryo is called a fetus – Sex is evident, and heartbeat can be detected Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings • Second trimester – Main changes involve an increase in size and a general refinement of human features – Placenta takes over secretion of progesterone and estrogen – Corpus luteum degenerates – Eyes are open, and teeth are forming Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings • Third trimester – Fetus grows rapidly and gains strength needed to survive outside uterus – Circulatory and respiratory system change to allow air breathing – Fetus gains ability to maintain its own temperature – Bones harden, muscles thicken Video: Ultrasound of Human Fetus 2 Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings 27.18 Childbirth is hormonally induced and occurs in three stages • The birth of a child is brought about by a series of strong, rhythmic contractions of the uterus • Several hormones play key roles, under positive feedback control – Estrogen – Oxytocin – Prostaglandins Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings LE 27-18a from ovaries Oxytocin from fetus and pituitary Induces oxytocin receptors on uterus Stimulates uterus to contract Stimulates placenta to make Prostaglandins Stimulate more contractions of uterus Positive feedback Estrogen • Labor occurs in three stages 1. Dilation of the cervix 2. Expulsion: delivery of the infant 3. Delivery of the placenta Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings LE 27-18b Placenta Umbilical cord Uterus Cervix Dilation of the cervix Expulsion: delivery of the infant Uterus Placenta (detaching) Umbilical cord Delivery of the placenta CONNECTION 27.19 Reproductive technology increases our reproductive options • Couples may be unable to conceive or bear a child for a variety of reasons – Males: low sperm count, defective sperm, impotence – Females: lack of ova, failure to ovulate, blocked oviducts, uterus won't support growing embryo Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings • Reproductive technologies can help many cases of infertility – Drug or hormone therapies for both sexes – Penile implants – Sperm, ova from donors – Assisted reproductive technology • In vitro fertilization – Very expensive – Entails some risks Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings