Download Litvinova Larisa - Immanuel Kant Baltic Federal University

CV Litvinova Larisa
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Larisa Sergeevna Litvinova
Immanuel Kant Baltic Federal University,
Laboratory of Immunology and Cell
Biotechnologies, 3 Botkina str., Kaliningrad,
236016, Russia
November 21, 1975
Single
Russia
+79114820489 (mobile)
+7 (4012) 595-595 (6631) (work)
[email protected]
EDUCATION:
1993-1999
1999-2002
2004-2007
Siberian State Medical University, Department of Pathophysiology,
Faculty of Biology and Medicine, Tomsk, Russia (Diploma of
Specialist)
PhD, Siberian State Medical University, Department
of Pathophysiology, Tomsk, Russia (Scientific Advisor - MD,
Professor Ryazantseva Natalia, thesis "Cytogenetic status of
peripheral blood lymphocytes in children with infectious
mononucleosis")
MD, Siberian State Medical University, Department of
Pathophysiology, Tomsk, Russian Federation (Scientific Advisor MD, Professor Ryazantseva Natalia, thesis "Mechanisms
of dysregulation of cooperation between eosinophils and immune
cells in blood eosinophilia")
PROFESSIONAL EXPERIENCE:
2007-2008
2008- up to date
Research fellow, Head of the inter-chair Laboratory of Molecular
Medicine, Siberian State Medical University, Tomsk, Russia
Projects: Mechanisms of dysregulation of cooperation between
eosinophils and immune cells in blood eosinophilia
Head of Laboratory Immunology and Cell Biotechnologies,
Immanuel Kant Baltic Federal University, Kaliningrad, Russian
Federation.
Projects:
1. Study the mechanisms of regulation of the immunological
memory in normal and pathological conditions:
Molecular mechanisms of violations of the structure, metabolism
and function of blood cells in pathology; Development of new
technologies to obtain individual universal T cell vaccines that are
effective in treating autoimmune diseases; Study of molecular
biological mechanisms of modulation of the immunological
memory in norm and autoimmune diseases; Study of cellular
and endocrine mechanisms of regulation of immunological
memory; The influence of steroid hormones on the functional
activity and processes of self-maintenance of T-cell immune
memory.
(Topicality
was
confirmed
by
obtaining
federal destination grants)
2. The study of molecular and immunological mechanisms of the
pathogenesis of the metabolic syndrome:
Investigation of immunopathological reactions in metabolic
syndrome; Study of the variability of mitochondrial DNA of
adipocytes in metabolic syndrome; Investigation of molecular and
cellular mechanisms of formation of chronic inflammation in
metabolic disorders; Search and identification of prognostic
significance of molecular and cellular markers in socially
significant diseases; Molecular and genetic mechanisms of
metabolic abnormalities in insulin resistance; Study of the
association of endothelial nitric oxide synthase gene
polymorphisms and metabolic syndrome in Russian population;
Study of gene expression of proinflammatory cytokines and
adipokines in metabolic syndrome. (Topicality was confirmed by
obtaining federal destination grants)
3. The study of molecular-genetic, morphological and functional
properties of multipotential mesenchymal stem cells (MMSCs):
The study of the regenerative capacity of multipotential
mesenchymal stem cells in various types of tissues; Development of
3D tissue-engineering constructs.
International scientific collaboration with Laboratory of
Experimental Modeling, Department of Cell and Tissue
Technologies, Institute of Genetic and Regenerative Medicine of
National Academy of Medical Sciences of Ukraine in Kiev,
Ukraine.
LABORATORY SKILLS AND COMPETENCES:
Basic skills:
Methods of isolation, cultivation, expansion, purification,
processing, cultivation, differentiation of cells, storage and
characterization culture of T-cells, eosinophils and mononuclear
cells.
Isolation, primary culture and expansion of mesenchymal
stromal cells from human adult adipose tissue, dermis. Comparative
analysis of the proliferation rate, and differentiation capacities of
cells from different sources, using qualitative and quantitative
methods. Immunophenotypic flow cytomery analysis of cells
during subculturing. Low temperature banking of cells. Clonal
analysis of mesenchymal stromal cells, using CFU-f assays.
Differentiation of isolated clones. Isolation and primary culture of
Technical skills:
Skills profile:
MSC from human adipose tissue and from rat bone marrow and
skin.
Hematologic research methods, methods of separation (density
gradient, the direct and indirect penning, immunomagnetic
separation), the definition of surface markers on different types of
cells (flow cytometry), coagulometry, agregatometry, biochemical
and enzyme immunoassay (all kinds), microscopy, Bio-Plex assay,
xCelligence and Cell-IQ system et al.
Fluent PC: OS Windows, all programs of package Microsoft
Office, Opera, Google et al., statistical software.
Knowledge of languages: English, Deutsch (understanding, reading,
speaking and writing)
Personal qualities: Ability to collaborate, perseverance, efficiency,
analytical thinking. Good communication skills.
CURRENT RESEARCH INTERESTS:
 Humoral and cellular regulation of adaptive immunity; immunopathological reactions in
socially significant diseases (metabolic syndrome, cardiovascular diseases, etc.).
 Regenerative medicine, cell-based therapy (multipotent adipose-derived stem cells), 3D
tissue engineered constructs for experimental and medical use.