Download FLUID THIOGLYCOLLATE MEDIUM w/ 0.5% BEEF EXTRACT BASE

FLUID THIOGLYCOLLATE MEDIUM w/ 0.5% BEEF EXTRACT BASE
INTENDED USE
Remel Fluid Thioglycollate Medium w/ 0.5% Beef Extract Base is a medium recommended for use in qualitative procedures to test for bacteria
in biological products.
SUMMARY AND EXPLANATION
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Thioglycollate Medium was first described by Brewer in 1940. This medium contained a small amount of agar and a reducing substance to
promote the growth of anaerobes. Fluid Thioglycollate Medium w/ 0.5% Beef Extract is recommended by the Animal and Plant Health Inspection
2
Service, U.S. Department of Agriculture (USDA).
PRINCIPLE
Sodium thioglycollate is a reducing agent which maintains a low oxygen tension in anaerobic media. A small amount of agar is added to
impede diffusion of oxygen. Casein peptone and cystine supply nitrogenous and carbon compounds, while the sodium chloride maintains
osmotic equilibrium. Beef extract provides nitrogenous substances and enhances organism recovery. Resazurin is added as an oxidationreduction indicator which turns pink when increased oxidation occurs. Yeast extract provides B-complex vitamins to enhance the growth of
bacteria.
REAGENTS (CLASSICAL FORMULA)*
Casein Peptone............................................................... 15.0
Beef Extract....................................................................... 5.0
Dextrose (anhydrous)........................................................ 5.0
Yeast Extract..................................................................... 5.0
g
g
g
g
Sodium Chloride .............................................................. 2.5 g
L-Cystine ......................................................................... 0.5 g
Sodium Thioglycollate ..................................................... 0.5 g
Resazurin ........................................................................ 1.0 mg
Agar ................................................................................0.75 g
pH 7.1 ± 0.2 @ 25°C
*Adjusted as required to meet performance standards.
PRECAUTIONS
This product is For Laboratory Use only. It is not intended for use in the diagnosis of disease or other conditions.
PREPARATION OF DEHYDRATED CULTURE MEDIUM
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Suspend 34.5 g of medium in 1000 ml of demineralized water.
Heat to boiling with agitation to completely dissolve.
Dispense into appropriate containers.
Sterilize by autoclaving at 121°C for 15 minutes or following established laboratory procedures.
PROCEDURE
1.
Consult current editions of appropriate references for the recommended procedure for sample preparation, inoculation, testing, and
interpretation.
QUALITY CONTROL
Each lot number of Fluid Thioglycollate Medium w/ 0.5% Beef Extract Base has been manufactured, packaged, and processed in accordance
with current Good Manufacturing Practice regulations. All lot numbers have been tested using the following quality control organisms and
have been found to be acceptable. Testing of control organisms should be performed in accordance with established laboratory quality control
procedures. If aberrant quality control results are noted, sample results should not be reported.
CONTROL
®
Bacillus subtilis ATCC 6633
®
Bacteroides vulgatus ATCC 8482
®
Candida albicans ATCC 10231
®
Clostridium sporogenes ATCC 11437
®
Kocuria rhizophila ATCC 9341
®
Streptococcus pyogenes ATCC 19615
INCUBATION
RESULTS
Aerobic, 24-48 h @ 33-37°C
Aerobic, 24-48 h @ 33-37°C
Aerobic, 24-48 h @ 33-37°C
Aerobic, 24-48 h @ 33-37°C
Aerobic, 24-48 h @ 33-37°C
Aerobic, 24-48 h @ 33-37°C
Growth
Growth
Growth
Growth
Growth
Growth
LIMITATIONS
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A slight turbidity (haziness) may be present due to the small amount of agar in the medium.
Do not boil more than once; frequent boiling results in the development of toxic products.
BIBLIOGRAPHY
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Brewer, J.H. 1940. JAMA. 115:598-600.
Federal Register. Animal and Plant Health Inspection Service, USDA. 9 CFR 113.26 (2010). Retrieved June 23, 2010 from: http://edocket.access.
gpo.gov/cfr_2010/janqtr/pdf/9cfr113.26.pdf.
Refer to the front of Remel Technical Manual of Microbiological Media for General Information regarding precautions, product storage and
deterioration, sample collection, storage and transportation, materials required, quality control, and limitations.
ATCC® is a registered trademark of American Type Culture Collection.
IFU 453472, Revised July 26, 2010
Printed in U.S.A.
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