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Name________________________ Concepts in Biology Exam #4 (12-15) Fall 2011 Mark each correct or true statement with a T and each incorrect or false statement with an F. (50) _______1. Bacterial plasmids are the most common vector used in recombinant DNA technology. _______2. A restriction enzyme is used to cut nucleic acids into specific fragments. _______3. DNA synthesized from an RNA molecule is referred to as recombinant DNA. _______4. The process used to “amplify” a small piece of DNA into many pieces is called the polymerase chain reaction. _______5. A collection of cloned DNA fragments that includes an organism’s entire genome is called the genomic library. _______6. Charles Darwin was the first person to conceive the idea of evolution. _______7. The development of pesticide resistant insects is an example of evolution occurring today. _______8. Charles Darwin actually became a famous naturalist while on the voyage of the Beagle. _______9. Lamarck’s mechanism of evolution was called the theory of acquired characteristics. _______10. The Origin of Species challenged the widely held belief of the idea of fixed species. _______11. Microevolution is usually documented in the fossil record and records the major changes in the history of life. _______12. The potential to interbreed defines a species in the biological species concept but does not apply to organisms that only undergo asexual reproduction. _______13. Hybrid breakdown, in which the hybrid is feeble, is a post-zygotic barrier. _______14. Reproductive barriers are most often pre-zygotic. _______15. Sympatric speciation is the origin of a new species without involving a geographical barrier. _______16. DNA technology raises both legal and ethical questions—few of which have clear answers. _______17. French naturalist George Buffon was one of the first to suggest that the Earth was only 6000 years old. _______18. The two models for the speed of evolution are the punctuated and the gradual. _______19. Mutations, random changes in the nucleotide sequence, can lead to the appearance of new genes. _______20. The binomial system of nomenclature states that every organism has at least two names, the genus name and the specific epithet or species name. _______21. A bacterium that is capable of causing disease is called a pathogen. _______22. Biogenesis refers to the concept that life could regularly arise from non-living matter. _______23. The endospore of some bacteria is considered a reproductive structure. _______24. Endotoxins are released as the bacterium grows in the infection. _______25. The use of microorganisms to remove pollutants from the environment is called bioremediation. Select the one best answer that either defines or completes the meaning of the given statement. (40) _______1. What feature of a DNA fragment causes it to move through a gel during electrophoresis? A. B. C. D. _______2. The enzyme necessary to produce cDNA A. B. C. D. _______3. The size of the fragments The double helix shape The hydrogen bonds between the base pairs The nucleotide sequence DNA polymerase RNA polymerase Reverse transcriptase All of the above are capable of producing a cDNA The world’s first genetically engineered pharmaceutical product was A. B. C. D. Subunit vaccine for anthrax Human growth hormone Hepatitis B vaccine Human insulin _______4. A radioactively tagged DNA fragment is called a A. B. C. D. _______5. The most commonly used organism in DNA engineering A. B. C. D. _______6. Individuals in a population differ in many heritable traits Evolution is goal oriented, and leads to perfectly adapted organisms Evolution refers to generation to generation changes in a population Evolution leads to unequal reproductive success in a population The change in the gene pool of a population A. B. C. D. _______9. Island isolation Gene pools Biogeography Fossil records All of the following are correct except A. B. C. D. _______8. Escherichia coli Saccharomyces cerevisaea Hemolphilus influenza Staphylococcus aureus Which of the following evidence’s evolution was not seen by Darwin on the voyage of the Beagle? A. B. C. D. _______7. Restriction enzyme Nucleic acid probe DNA vector probe Plasmid Hardy-Weinberg equilibrium Genetic drift Gene flow Darwinian fitness Natural selection that shifts the overall makeup of a population by selecting opposite extremes is called A. B. C. D. Disruptive selection Directional selection Stabilizing selection Destabilizing selection _______10. Which of the following statements is true about a population in Hardy-Weinberg equilibrium? A. B. C. D. _______11. Mass extinctions A. B. C. D. _______12. Clade Genus Population Homologue The oldest geologic time A. B. C. D. _______15. All likeness is thought to be inherited from a common ancestor Results from analogous adaptations in different evolutionary branches Is demonstrated best by fossil records All of the above are correct An ancestral species and all its descendants A. B. C. D. _______14. Cut the number of species to the few survivors left today Resulted mainly from the separation of the continents Occurred regularly, about every million years Were followed by diversification of the survivors Convergent evolution A. B. C. D. _______13. The population is quite small The population is not evolving Natural selection is occurring, but at a slow rate Heritable variation is occurring Cenozoic era Mesozoic era Paleozoic era Precambrian era Which of the following is true concerning the tempo of speciation? A. B. C. D. Punctuated equilibrium exhibits long periods where little change occurs In punctuated equilibrium there are no transitional stages Gradual diversification occurs in the graduated tempo model All of the above are correct _______16. The binomial system of nomenclature was proposed by A. B. C. D. _______17. Which Kingdom was “lost” when the three domain system began to be used in classification? A. B. C. D. _______18. The percentage of homozygous dominant individuals The percentage of recessive alleles The percentage of heterozygotes in the population The percentage of recessive individuals Of these listed, the most inclusive taxonomic level is A. B. C. D. _______21. Genera Orders Families Phyla In the Hardy Weinberg equation, 2pq represents A. B. C. D. _______20. Protista Monera Animalia Fungi Similar genera are grouped together into A. B. C. D. _______19. Buffon Lamarck Darwin Linnaeus genus order phylum family Which of the following does not belong to the Kingdom Protista? A. B. C. D. Cellular slime molds Trypanosomes Amoebas Ciliates like Paramecium _______22. The theory that tries to explain the evolution of eukaryotic cells A. B. C. D. _______23. The common shape(s) of bacteria A. B. C. D. _______24. Abiogenesis Biogenesis Endosymbiosis Parasitism Spirals Coccus forms Bacillus forms All of the above are correct Put the following stages of the hypothesis for the origin of life in order 1. 2. 3. 4. A. B. C. D. _______25. Abiotic synthesis of polymers Abiotic synthesis of monomers Self-replicating molecules Pre-cell formation 1,2,4,3 4,3,2,1 2,1,4,3 2,1,3,4 The extreme bacteria, those that live in extreme environments, are found in the A. B. C. D. Monera Archae True bacteria All of the above are correct Required Essay: Given the following information, calculate the values of q and p; and calculate the values of p2 and q2 and 2pq. A population has a certain recessive trait. This trait is present in 36% of the population. Given that p + q = 1 and p2 + 2pq + q2 = 1. (10) Choose one of the following essay questions. You may do the second question as a bonus. (10) List and discuss the two observations and the inference or conclusion drawn from them that Darwin proposed in natural selection. Outline the basic process of recombinant DNA technology and cite 5 practical applications of that research. Define and discuss the four modes of nutrition of all organisms. Give examples.