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JANET C. LINDOW, PhD Current Titles: Associate Research Scientist School of Public Health – Department of Epidemiology of Microbial Diseases Yale University Visiting Research Scientist (Pesquisador Visitante) Laboratory of Pathology and Molecular Biology Oswaldo Cruz Foundation (Fiocruz) Current Address: 60 College St, Rm 319 New Haven, CT 06510 Phone: (203) 785-3927 Brazil Phone: +55-71-9151-1025 E-mail:[email protected] EDUCATION 1996 2002 University of Illinois - Urbana Massachusetts Institute of Technology B.S. Ph.D. Microbiology (minor: Biochemistry) Biology APPOINTMENTS 1992-1996 Department of Microbiology University of Illinois Urbana, IL Research Assistant, Dr. Charles Miller (Chair) 1996-2002 Department of Biology Massachusetts Institute of Technology Cambridge, MA Graduate Student, Dr. Alan Grossman 2003-2006 Department of Biochemistry University of Colorado – HSC Denver, CO Post-Doctoral Fellow, Dr. Charles McHenry 2006-2007 Department of Chemistry University of Colorado – Boulder Boulder, CO Post-Doctoral Fellow, Dr. Charles McHenry 2008- 2010 Department of Medicine University of Vermont Burlington, VT Post-Doctoral Associate, Dr. Beth Kirkpatrick 2010-2012 Department of Medicine University of Vermont Burlington, VT Research Associate, Dr. Beth Kirkpatrick 2012-2013 Department of Medicine University of Vermont Burlington, VT Assistant Professor 2013-present Department of Epidemiology of Microbial Diseases Associate Research Scientist Yale University New Haven, CT 2013-present Laboratory of Pathology and Molecular Biology Oswaldo Cruz Foundation (Fiocruz) Salvador, Bahia, Brazil Janet Lindow, PhD Visiting Research Scientist Curriculum vitae HONORS AND AWARDS 1992-1996 1994-1995 1995 1995-1996 1996 1996 1996 1996 1996 1996-1999 2013-2016 2013-2014 2014-2015 James Scholar (Academic Excellence Award), University of Illinois Microbiology summer research fellowship, University of Illinois Clark Microbiology Award for best academic record, University of Illinois American Society for Microbiology Undergraduate Research Fellowship Clark Microbiology Award for best academic record, University of Illinois School of Life Sciences Best Undergraduate Thesis Award, University of Illinois School of Life Sciences All School Distinction Award, University of Illinois Bronze Tablet (for 5.0 GPA), University of Illinois B.S. summa cum laude in School of Life Sciences, University of Illinois National Science Foundation Graduate Research Fellow, MIT Science without Borders Junior Investigator Fellowship, Brazilian Ministry of Science and Technology ASTMH Gorgas Memorial Institution Research Award Global Health Equity Scholar Faculty Fellow PROFESSIONAL ACTIVITIES Membership in Professional Organizations 1995-present 1999-2000 2008-present 2009-2012 2011-2014 Member. American Society for Microbiology Representative. MIT Department of Biology Graduate Education Committee Member. American Society for Tropical Medicine and Hygiene Member. Society for Mucosal Immunology Member. Infectious Diseases Society of America 2012-present Member. American Association for the Advancement of Science Ad Hoc Reviewer Journal of Bacteriology Molecular Microbiology Journal of Infectious Diseases PLoS Neglected Tropical Diseases Clinical and Vaccine Immunology Journal of Clinical Microbiology Drug Design, Development, and Therapy International Journal of Infectious Diseases SERVICE UVM College of Medicine Service 2009-2010 Post-doctoral representative on Graduate Education Committee University Service 2011-2013 University of Vermont Institutional Biosafety Committee member Janet Lindow, PhD Curriculum vitae Community/Public Service October 15, 2009 Speaker. “The making of a scientist,” Area Health Education Center. Vermont Technical College, Randolph, VT. November 7, 2009 Speaker. “The road to becoming a scientist,” Area Health Education Center. University of Vermont, Burlington, VT. March 17, 2010 Scientist mentor for a day to Paige Wood. University of Vermont, Burlington, VT. TEACHING (MIT) Year Course 1998 Spring 7.014 Introductory Biology 1999 Fall 7.18 Experimental Biology 1999-2000 Arithmetic and Basic English Nature of Contribution Hrs/Semester Teaching Assistant 154 Teaching Assistant 112 Volunteer Teacher 30 Class size 50/2 sections 15 10-15 TEACHING (Center for Excellence in Education - University of Gaborone, Botswana) Year Course Nature of Contribution Hrs 2003 (June) Biology- Scientific Method Co-Instructor 20hr/wk Class size 16-31 TEACHING (University of Vermont) Year Course Nature of Contribution 2009 Fall MMG065 Microbiology and Pathogenesis Guest Lecturer 2010 Fall MMG211 Prokaryote Molecular Genetics Guest Lecturer 2011 Spring MMG320 Cellular Microbiology Guest Lecturer Class size ~60 ~12 ~9 Hrs/Semester 2 1 2.5 MENTORING Year 2000 2000 2005 2009 2009 2010 2010 2010 2010 2010 2010 2011 2012 2012 2013 2013 2013 2013 2013 2014 2014 Name Milan deVries Megan Rokop Uma Sharma Amanda Ochoa Sarah Gillett Kelly Fimlaid Jake Shea Kaila Aeillo Nathan Borochoff-Porte Ellen Frasier Kelly Fimlaid Teresa Burton Ben McElvany Katrin Sadigh Guilherme Araujo Jaqueline Soares Laiara Lopes Camila Jacob Carolina Lessa-Aquino Ricardo Moreira de Andrade Vinicius Machado Janet Lindow, PhD Time 1 month 1 month 1 semester 1 semester 3 months 3 months 3 months 3 months 1.5 years 3 years 2 years 3 months 1 year 1 month ongoing ongoing 1 year ongoing ongoing 5 months ongoing Nature of Mentoring University Rotation supervisor MIT Rotation supervisor MIT Research supervisor University of Colorado Undergraduate research project UVM Rotation advisor/supervisor UVM Rotation advisor/supervisor UVM Summer research project UVM Summer research project UVM Research supervisor UVM Research supervisor UVM Co-advisor UVM Rotation advisor/supervisor UVM Research supervisor UVM Medical rotation supervisor Boston University Supervisor Fiocruz Advisor Fiocruz Research supervisor Fiocruz Advisor Fiocruz Collaboration research supervisor Fiocruz Advisor Fiocruz Supervisor Fiocruz Curriculum vitae 2014 Milena Kelly 4 months Research supervisor Fiocruz 5 months ongoing Research supervisor Research supervisor Yale Fiocruz Graduate Students 2013 2013 Annie Tsay Alcineia Damiao PUBLICATIONS Peer-reviewed articles: 1) Lindow JC, Dohrmann PR, and McHenry CS. DNA Polymerase Alpha Subunit Residues and Interactions Required for Efficient Initiation Complex Formation Identified by a Genetic Screen. J Biol Chem 2015; In press. 2) Backstedt BT, Buyuktanir O, Lindow JC, Wunder EA, Reis MG, Usmani-Brown S, Ledizet M, Ko AI, and U Pal. Efficient detection of pathogenic leptospires using 16S ribosomal RNA. PLoS One 2015; In press. 3) Lessa-Aquino C, Wunder EA Jr, Lindow JC, Rodrigues CB, Pablo J, Nakajima R, Jasinskas A, Liang L, Reis MG, Ko AI, Medeiros MA, and PL Felgner. Proteomic Features Predict Seroreactivity against Leptospiral Antigens in Leptospirosis Patients. J Proteome Res 2015; 14(1):549-56. PMID: 25358092. PMCID: PMC4286151. 4) Weiskopf D, Angelo MA, Bangs D, Signey J, Paul S, Peters B, de Silva AD, Lindow JC, Diehl SA,Whitehead SS, Durbin AP, Kirkpatrick BD, and A Sette. The human CD8+ T cell responses induced by a live attenuated tetravalent dengue vaccine are directed against highly conserved epitopes. J Virol 2015; 89(1):120-8. PMID: 25320311. PMCID: PMC4301095. 5) Fimlaid KA, Lindow JC, Tribble DR, Bunn JY, Maue AC, and BD Kirkpatrick. Peripheral CD4+ T cell Cytokine Responses Following Human Challenge and Re-challenge with Campylobacter jejuni. PLoS One. 2014; 9(11):e112513. PMID: 25397604. 6) Lindow, JC, Durbin AP, Whitehead SS, Carmolli MP, Pierce KK, and Kirkpatrick BD. Vaccination with 10 live dengue viruses leads to distinct immunologic and virologic profiles. J Infect Dis 2013; 207(6):957-65. PMCID: PMC3777849 7) Durbin AP, Kirkpatrick BD, Pierce K, Larsson CJ, Lindow JC, et al. A single dose of four separate live attenuated tetravalent dengue vaccine admixtures is safe and highly immunogenic in flavivirus-naïve adult volunteers. J Infect Dis 2013; 207(6):957-65. PMCID: PMC3571448 8) Lindow, JC, Borochoff-Porte N, Durbin AP, Whitehead SS, Fimlaid KA, Bunn JY, and BD Kirkpatrick. Primary Vaccination with Low Dose Live Dengue 1 Virus Generates a Proinflammatory, Multifunctional T Cell Response in Humans. PLoS Negl Trop Dis 2012; 6(7): e1742. PMCID: PMC3398956 9) Lindow JC, Fimlaid, KA, Bunn, JY and Kirkpatrick BD. Antibodies in action: the role of human opsonins in killing Salmonella enterica serovar Typhi. Inf Imm 2011; 79 (8): 3188-3194. PMCID: PMC3147595 10) Lindow JC*, Poly, F*, Tribble DR, Guerry P, Carmolli MP, Baqar S, Porter CK, Pierce KK, Darsley MJ, Sadigh KS, Dill EA, The Campylobacter Study Team, Kirkpatrick BD. Caught in the act: In vivo development of macrolide resistance to Campylobacter jejuni infection. J Clin Microbiol 2010; 48 (8): 3012-5. PMCID: PMC2916616 * Co-first authors: JCL reviewed and processed data and wrote the manuscript. FP performed the assays in the manuscript. Janet Lindow, PhD Curriculum vitae 11) Lyon CE, Sadigh KS, Carmolli MP, Harro C, Sheldon E, Lindow JC, Larsson CJ, Schwartz HI, Martinez T, Feller A, Ventrone CH, Sack DA, DeNearing B, Fingar A, Pierce K, Beardsley E, Kilonzo B, Kirkpatrick BD. The single oral dose typhoid vaccine, M01ZH09, is safe and immunogenic at doses up to 1.7 x 1010 colony-forming units. Vaccine 2010; 28:3602-8. PMID: 20188175 12) Carmolli M, Duggal P, Haque R, Lindow J, Mondal D, Petri WA, Jr, Mourningstar P , Larsson CJ, Sreenivasan M, Khan S , Kirkpatrick BD. Deficient serum MBL and MBL2 polymorphisms increase the risk of single and recurrent Cryptosporidium infections in young children. J Infect Dis 2009; 49 (10):1512-9. PMCID: PMC3400050 13) Lindow JC, Britton RA, and Grossman AD. Structural Maintenance of Chromosomes protein of Bacillus subtilis affects supercoiling in vivo. J Bacteriol 2002; 184(19):5317-5322. PMCID: PMC135370 14) Lindow JC, Kuwano M, Moriya S, and Grossman AD. Subcellular localization of the Bacillus subtilis Structural Maintenance of Chromosomes (SMC) protein. Mol Microbiol 2002; 46:997-1009. PMID: 12421306 In preparation for peer-reviewed publication: 15) Lindow JC, Wunder EA, Popper SJ, Min J, Fiocruz Leptospirosis Group, Lee PJ, Relman DA, Kim CC, Araújo G, Reis MG, and AI Ko. Altered immune responses in patients with leptospirosis are associated with death. In preparation. 16) Lindow JC, Montgomery RR, Shaw A, Tsay, A, Araújo G, EG Reis, MG Reis, and AI Ko. Altered neutrophil function is associated with poor patient outcomes in leptospirosis. In preparation. 17) Lindow JC, Li Y, Montgomery RR, Sarkar K, Reis MG, and AI Ko. Leptospirosis patients with poor outcomes lack robust adaptive immune memory responses. In preparation. 18) Lindow JC, Lessa-Aquino C, Wunder EA Jr, Pablo J, Nakajima R, Jasinskas A, Liang L, Reis MG, Medeiros MA, Felgner PL, and Ai Ko. Identification of Leptospira antigens associated with poor disease outcomes in patients with leptospirosis. In preparation. 19) Lindow JC, Ochsner UA, Pope C, Gogain GC, and McHenry CS. A Second DnaE-like gene in Pseudomonas aeruginosa encodes ImuC, a polymerase required for induced mutagenesis that functions in concert with a catalytically inactive PolY-like protein. In preparation. Recent Abstracts: 1) Lindow JC, Wunder EA, Popper SJ, Min J, Lee PJ, Reis MG, AI Ko, and Fiocruz-Yale Slum Health Research Group. Defect in the cathelicidin response leads to poor outcomes during human leptospirosis. Gordon Research Conference in Tropical Infectious Diseases, Galveston, TX, 2015. 2) Popper SJ, Lindow JC, Strouts FR, Sebastian E, Durbin AP, Kirkpatrick BD, Whitehead SS, and DA Relman. Early transcriptional responses that correlate with neutralizing antibody development in dengue vaccine recipients. American Society Tropical Medicine Hygiene, Washington D.C. 2013 3) Pierce KK, Kirkpatrick BD,Durbin AP, Lindow JC, Carmolli MP, Shaffer D, Colgate R, Larsson CJ, Wanionek K, Walsh MC, Andrada A, and Whitehead SS. Evaluation of low dose monovalent dengue vaccines in human volunteers. American Society Tropical Medicine Hygiene, Philadelphia. 2011. 4) Kirkpatrick BD, Durbin AP, Pierce KP, Lindow JC, Elwood D, Wanionek K, Andrada A, Carmolli M, and Whitehead SS. Evaluation of the safety and immunogenicity of TETRAVAX-DV, a live attenuated tetravalent dengue vaccine. Dengue Vaccine Initiative, Washington DC. 2011. Janet Lindow, PhD Curriculum vitae 5) Lindow JC, Fimlaid KA, and BD. Kirkpatrick. Opsonins clear Salmonella Typhi in humans. UVM Department of Medicine Research Day. 2010. 6) Lindow JC, Fimlaid KA, and BD. Kirkpatrick. Antibodies in action: role of opsonins in clearing Salmonella Typhi in humans. The American Society Tropical Medicine and Hygiene 59th Annual Meeting, Atlanta, GA. 2010. 7) Lyon CE, Carmolli M, Lindow J, Harro C, Sheldon E, Larsson CJ, Ventrone C, Sadigh K, Ventrone CH, Pierce KK, Fingar A, Dill E., and Kirkpatrick BD. The novel, single oral dose typhoid vaccine M01ZH09 is safe and immunogenic at doses up to 1.7 x1010 Colony-forming units. Vaccines for Enteric Diseases Conference, Malaga, Spain. 2009. Oral Presentation. LECTURES International Meetings (Invited Speaker): 2001 “Role of Bacillus subtilis SMC in chromosome compaction,” Keystone Symposia, Bacterial Chromosomes, Santa Fe, NM. National and International Seminars (Invited Speaker): 2015 “Altered immune responses in patients with leptospirosis lead to death,” Gordon Research Seminar – Tropical Infectious Diseases, Galveston, TX. 2013 “Vaccines and T cells: a search for a correlate of protection for dengue disease,” Oswaldo Cruz Foundation (CNpGM), Salvador, Brazil. 2013 “Characterizing the human immune response to Leptospira infection,” Oswaldo Cruz Foundation (CNpGM), Salvador, Brazil. 2013 “Vaccines and T cells: a search for a correlate of protection for dengue disease,” Yale School of Public Health, New Haven, CT. 2012 “Vaccines and T cells: a search for a correlate of protection for dengue disease,” University of Rhode Island, Providence, RI. 2011 “Cell mediated immune responses to dengue viruses,” University of California, Berkeley, CA. 2011 “Identifying immunoprofiles predictive of severe dengue disease or correlated with dengue serotype,” Laboratory of Infectious Diseases, NIH, Bethesda, MD. 2007 “Genetic selection for mutations within regions of E. coli dnaE that encode sites of interaction with other proteins,” Albert Einstein College of Medicine, Bronx, NY. 2003 “A role for the bacterial Structural Maintenance of Chromosomes in chromosome compaction,” University of Colorado – Health Sciences Center, Denver, CO. 2003 “Mycobacterium bovis infection in Kruger National Park, South Africa,” Colorado State University, Ft. Collins, CO. 2003 “A role for the bacterial Structural Maintenance of Chromosomes in chromosome compaction,” University of Colorado – Health Sciences Center, Denver, CO. 2001 “A role for the bacterial Structural Maintenance of Chromosomes in chromosome compaction,” Harvard Medical School - Longwood Campus, Boston, MA. 2001 “A role for the bacterial Structural Maintenance of Chromosomes in chromosome compaction,” Harvard Medical School – Massachusetts General Hospital, Boston, MA. Janet Lindow, PhD Curriculum vitae 2001 “A role for the bacterial Structural Maintenance of Chromosomes in chromosome compaction,” Baylor College of Medicine, Houston, TX. 2001 “A role for the bacterial Structural Maintenance of Chromosomes in chromosome compaction,” UCSF, San Francisco, CA. Local/Regional Meetings (Speaker): 2012 “Is there a reversible functional immune disorder in malnourished children?” ID Research Conference, FAHC, Burlington, VT. 2012 “What causes vaccine underperformance in the developing world?” VCIID seminar, University of Vermont, Burlington, VT. 2012* “Vaccines and T cells: a search for a correlate of protection for dengue disease,” ID Research Conference, FAHC, Burlington, VT. 2012* “Vaccines and T cells: a search for a correlate of protection for dengue disease,” MMG Departmental Seminar, University of Vermont, Burlington, VT. 2012 “Dengue serotype-specific immune responses in humans,” Department of Medicine, University of Vermont, Burlington, VT. 2011 “Dengue R01 grant overview,” Vaccine Testing Center, University of Vermont, Burlington, VT. 2011 “Food poisoning and dengue fever…what a combo,” MMG Department Retreat, Grand Isle, VT. 2011 “Identifying immunoprofiles predictive of severe dengue disease,” MMG supergroup meeting, University of Vermont, Burlington, VT. 2011 “Kinetics of T cell responses following dengue vaccination,” Vaccine Testing Center, University of Vermont, Burlington, VT. 2011 “Identifying immunoprofiles predictive of severe dengue disease,” VCIID seminar, University of Vermont, Burlington, VT. 2010 “Bacteria, Viruses and Vaccines, Oh My,” MMG retreat, University of Vermont, Grand Isle, VT. 2010 “Clearance of Salmonella Typhi infections in humans: opsonins at work,” VCIID seminar, University of Vermont, Burlington, VT. 2010 “The Role of Opsonins in S. Typhi Infections in Humans,” MMG supergroup meeting, University of Vermont, Burlington, VT. 2010* “Clearance of Salmonella Typhi infections in humans: opsonins at work,” MMG seminar, University of Vermont, Burlington, VT. 2010 “Clearance of Salmonella Typhi infections in humans: opsonins at work,” Vaccine Testing Center, University of Vermont, Burlington, VT. 2010 “Writing scientific papers,” Vaccine Testing Center, University of Vermont, Burlington, VT. 2009* “The road to becoming a scientist,” AHEC. University of Vermont, Burlington, VT. 2009* “The making of a scientist,” AHEC.Vermont Technical College, Randolph, VT. 2009 “Immunization with a new typhoid fever vaccine increases macrophage uptake and killing of wildtype S. Typhi in humans,” MMG supergroup meeting, University of Vermont, Burlington, VT. 2009 “Dengue and dengue vaccines,” Infectious Disease Unit Research Conference, FAHC, Burlington, VT. Janet Lindow, PhD Curriculum vitae 2008 “Dengue viruses: vaccine development and immunology studies,” VCIID seminar, University of Vermont, Burlington, VT. 2001* “Bacillus subtilis SMC affects supercoiling in vivo,” New England Spores Meeting, Boston, MA. 2001* “Bacillus subtilis SMC affects supercoiling in vivo,” Boston Bacterial Meeting, Boston, MA 2001 “The role of SMC in chromosome compaction,” MIT Motility & Morphology Club, Cambridge, MA. 2001 “Bacillus subtilis SMC affects supercoiling in vivo,” MIT Biology Dept. Retreat, Falmouth, MA. 2000 “A role for a bacterial SMC in chromosome compaction,” MIT Cell Cycle Club, Cambridge, MA. * Denotes invited speaker for Regional/Local Meetings Poster Presentations*: National/International Meetings: 2010 “Antibodies in action: the role of opsonins in clearing Salmonella Typhi in humans,” ASTMH Meeting, Atlanta, GA. 2005 “Genetic selection for mutations within regions of E. coli dnaE that encode sites of interaction with other proteins,” Keystone Meeting: DNA Replication and Repair, Keystone, CO. 1999 “Role of Bacillus subtilis SMC in chromosome compaction,” Gordon Research Conference, Plasmid and Chromosome Dynamics, New London, NH. Local/Regional Meetings: 2005 “Isolation and biochemical characterization of dnaE403DE suppressors that encode sites of interaction with other replication proteins,” Dept. of Biochemistry Retreat, University of Colorado, Estes Park, CO. 2003 “Purification and characterization of B. subtilis PolC,” Dept. of Biochemistry Retreat, University of Colorado, Estes Park, CO. * Pre-2003 poster presentations not listed. FUNDING Current Ciencias sem Fronteiras (Lindow, JC) Role: PI 10/03/2013-10/01/2016 Fiocruz CNPq – Salvador, Brazil Immunopathogenesis and Immune Memory in Human Leptospirosis The project will identify host factors that predispose individuals to severe leptospirosis and will provide evidence for the role of the immune response, particularly those driven by macrophages, neutrophils and T cells, as immunopathogenic processes, which in turn, may inform new therapeutic approaches and promote vaccine design. Gorgas Memorial Institute Research Award (Lindow, JC) Role PI 03/01/2014-01/01/2015 ASTMH Immunopathogenesis in Human Leptospirosis This project proposes to characterize the human immune response in a cohort of hospitalized leptospirosis patients with the aim of identifying the mechanism causing pathology in severe disease. 5 U01 AI088752-02 (Ko, AI) Role Co-I NIH/NIAID Janet Lindow, PhD 08/10/2010-07/31/2015 Curriculum vitae Disease determinants for urban leptospirosis The goal of this ICIDR program is to apply approaches in genomics and proteomics to field studies in Brazil in order to characterize the disease determinants of leptospirosis and identify novel intervention strategies. Global Health Equity Scholar Junior Faculty Fellow (Lindow, JC) Role PI 04/01/2014-01/01/2015 Immunopathogenesis in Human Leptospirosis This project proposes to characterize the human immune response in a cohort of hospitalized leptospirosis patients with the aim of identifying the mechanism causing pathology in severe disease. Pending U01 AI-14-001 (Ko, AI) Role Co-I NIH/NIAID Disease determinants for urban leptospirosis The goal of this ICIDR program is to apply approaches in genomics and proteomics to field studies in Brazil in order to characterize the disease determinants of leptospirosis and identify novel intervention strategies. Completed Immunology COBRE Pilot Project Grant (Lindow, JC) Role: PI 01/01/2011-07/01/2011 P20RR021905 (Ralph Budd, PI of COBRE) The kinetics of human cellular immune responses to dengue infections following initial infection and heterologous re-infection The major goal of this project was to define T cell responses to each of the four dengue virus serotypes using specimens from human clinical trials of live, attenuated dengue vaccines. F32 Ruth L. Kirschstein National Research Service Award (Lindow, JC) 04/01/2005-03/31/2007 GM074532-02 Identification and characterization of the Gram positive Bacillus subtilis replicative DNA polymerase holoenzyme The goal of the study was to determine the role of DnaE in replication in Bacillus subtilis. The work proposed to biochemically characterize the function of the B. subtilis DnaE at the replication fork. National Science Foundation Graduate Fellowship 08/01/1996-07/31/1999 Competitive Graduate Research Award This award supported my graduate research in defining the role of the Bacillus subtilis Structural Maintenance of Chromosomes protein in chromosome dynamics. Janet Lindow, PhD Curriculum vitae