Download QUERCETIN CONJUGATED GOLD NANOPARTICLE MAY INDUCE

QUERCETIN CONJUGATED GOLD NANOPARTICLE MAY INDUCE
APOPTOSIS IN HUMAN BREAST CANCER CELLS
Balakrishnan S# Firdous Ahmad Bhat, Raja Singh P, Arunakaran J*
Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences,
University of Madras, Taramani, Chennai - 600113.
Email ID: [email protected]*, [email protected]#
Background: Breast cancer is the most common invasive cancer and the second leading
cause of death among women. AuNPs (Gold nanoparticles) have attracted a great deal of
attention due to their chemical inertness and biocompatibility, making them suitable for
biomedical applications like cancer treatment and drug delivery systems. Quercetin is a wellknown bioflavonoid, shown to have anti-cancer, antioxidant and anti-inflammatory activities.
Bioflavonoid conjugated Gold nanoparticles can be delivered to specific target sites, paving
the way for targeted drug delivery, thereby minimizing the side effects and maximizing drug
efficacy.
Aim: The purpose of study is to investigate the effects of quercetin conjugated gold
nanoparticles (Q+AuNPs) on human breast cancer cells.
Methods: Quercetin conjugated gold nanoparticles were synthesized according to previous
study and Q+AuNPs were characterized by UV, FT-IR, HR-TEM, EF-SEM, EDAX, XRD,
AFM, Particle size analyzer and zeta potential measurement. Cell viability was assessed by
MTT assay. DAPI staining, AO/EtBr dual staining and DNA fragmentation was used to
visualize apoptotic cells.
Results: The growth inhibitory effect of Q+AuNPs was assessed by MTT assay. Q+AuNPs
treatment was determined by AO/EtBr dual staining, DAPI staining and DNA fragmentation.
Conclusions: It is concluded that quercetin conjugated gold nanopartilcles may induce
apoptosis in human breast cancer cells.