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Membrane Transport and Membrane
Dr. Michael P. Gillespie
Action Potential
 Have the ability to produce action potentials or impulses
(electrical excitability) in response to a stimulus.
 An action potential is an electrical signal that propagates
from one point to the next along the plasma membrane of a
 A stimulus is any change in the environment that is strong
enough to initiate an action potential.
Parts of a Neuron
 Cell Body
 Dendrites
 Axon
Parts of a Neuron (Cell Body)
 Cell body (perikaryon or soma).
 Contains the nucleus surrounded by cytoplasm which contains
the organelles.
 Clusters of rough ER called Nissl bodies (produce proteins to
grow and repair damaged nerves)
Parts of a Neuron (Nerve Fiber)
 Nerve fiber – any neuronal process that emerges from
the cell body of a neuron.
 Dendrites
 Axon
Parts of a Neuron (Dendrites)
 Dendrites (= little trees).
 The receiving (input) portion of a neuron.
 Short, tapering, and highly branched.
Parts of a Neuron (Axon)
 Axon (= axis).
 Each nerve contains a single axon.
 The axon propagates nerve impulses toward another neuron, muscle
fiber, or gland cell.
 Long, thin, cylindrical projection that often joins the cell body at a coneshaped elevation called the axon hillock (= small hill).
 The part of the axon closest to the hillock is the initial segment.
 The junction between the axon hillock and the initial segment is the
trigger zone (nerve impulses arise here).
 The cytoplasm of the axon is the axoplasm and is surrounded by a
plasma membrane known as the axolemma (lemma = sheath).
 The synapse is the site of communication between two
neurons or between a neuron and an effector cell.
 Synaptic end bulbs and varicosities contain synaptic
vesicles that store a chemical neurotransmitter.
 The myelin sheath is a lipid and protein covering. It is
produced by the neuroglia.
 The sheath electrically insulates the axon of a neuron.
 The sheath increases the speed of nerve impulse
 The amount of myelin increases from birth on.
 Axons without a covering are unmyelinated. Axons
with a covering are myelinated.
Myelination Continued…
 Two types of neuroglial cells produce myelination.
 Schwann cells – located in the PNS.
 Oligodendrocytes – located in the CNS.
Neurolemma (Sheath of Schwann)
 The neurolemma (sheath of Schwann) is the outer
nucleated cytoplasmic layer of the Schwann cell.
 It encloses the myelin sheath.
 It is only found around the axons of the PNS.
 If the axon is injured, the neurolemma forms a regeneration
tube that guides and stimulates re-growth of the axon.
Nodes of Ranvier
 The nodes of Ranvier are gaps in the myelin sheath at
intervals along the axon.
 Each Schwann cell wraps one axon segment between two
 The electrical impulse jumps from node to node to speed up
the propagation
 Nodes of Ranvier are present in the CNS, but fewer in
 Demyelination is the loss or destruction of the myelin sheaths
around axons.
 It occurs as the result of disorders such as multiple sclerosis
or Tay-Sachs disease.
 Radiation and chemotherapy can also damage the myelin
 Demyelination can deteriorate the affected nerves.
Electrical Signals in Neurons
 Neurons are electrically excitable and communicate with
one another using 2 types of electrical signals.
 Graded potentials (short distance communication).
 Action potentials ((long distance communication).
 The plasma membrane exhibits a membrane
potential. The membrane potential is an electrical
voltage difference across the membrane.
Electrical Signals in Neurons
 The voltage is termed the resting membrane potential.
 The flow of charged particles across the membrane is called
 In living cells, the flow of ions constitutes the electrical
Ion Channels
 The plasma membrane contains many different kinds of ion
 The lipid bilayer of the plasma membrane is a good electrical
 The main paths for flow of current across the membrane are
ion channels.
Ion Channels
 When ion channels are open, they allow specific ions to
move across the plasma membrane down their
electrochemical gradient.
 Ions move from greater areas of concentration to lesser areas of
 Positively charged cations move towards a negatively charged
area and negatively charged anions move towards a positively
charged area.
 As they move, they change the membrane potential.
Ion Channel “Gates”
 Ion channels open and close due to the presence of “gates”.
 The gate is part of a channel protein that can seal the channel
pore shut or move aside to open the pore.
Types of Ion Channels
 Leakage channels
 Ligand-gated channel
 Mechanically gated channel
 Voltage gated channel
Leakage Channels
 Leakage channels – gates randomly alternate between open and
closed positions.
 More potassium ion (K+) leakage channels than sodium (Na+)
leakage channels.
 The potassium ion leakage channels are leakier than the sodium
ion leakage channels.
Ligand-gated Channel
 Ligand-gated channels – open and close in response to a specific
chemical stimulus.
 Neurotransmitters, hormones, and certain ions can act as the
chemical stimulus that opens or closes these channels.
Mechanically Gated Channel
 Mechanically gated channels – opens or closes in response to
mechanical stimulation.
 Vibration, touch, pressure, or tissue stretching can all distort the
channel from its resting position, opening the gate.
Voltage-gated Channel
 Voltage-gated channels – opens in response to a change in
membrane potential (voltage).
 These channels participate in the generation and conduction of
action potentials.
 Concentration Gradient – A difference in the concentration
of a chemical from one place to another.
 Electrochemical Gradient – The combination of the effects of
the concentration gradient and the membrane potential.
Transport Across the Membrane
 Passive Transport – does not require cellular energy.
 Substances move down their concentration or electrochemical
gradients using only their own kinetic energy.
 Active Transport – requires cellular energy in the form of
3 Types of Passive Transport
 Diffusion through the lipid bilayer.
 Diffusion through membrane channels.
 Facilitated diffusion.
 Materials diffuse from areas of high concentration to areas of
low concentration.
 The move down their concentration gradient.
 Equilibrium – molecules are mixed uniformly throughout
the solution.
Factors Influencing Diffusion
 Steepness of the concentration gradient.
 Temperature.
 Mass of the diffusing substance,
 Surface area.
 Diffusion distance.
Resting Membrane Potential
 The resting membrane potential occurs due to a buildup
of negative ions in the cytosol along the inside of the
membrane and positive ions in the extracellular fluid along
the outside of the membrane.
 The potential energy is measured in millivolts (mV).
Resting Membrane Potential
 In neurons, the resting membrane potential ranges from –40
to –90 mV. Typically –70 mV.
 The minus sign indicates that the inside of the cell is negative
compared to the outside.
 A cell that exhibits a membrane potential is polarized.
 The potential exists because of a small buildup of negative
ions in the cytosol along the inside of the membrane and
positive ions in the extracellular fluid along the membrane.
Electrochemical Gradient
 An electrical difference and a concentration difference across
the membrane.
Factors Producing the Resting
Membrane Potential
 Unequal distribution of ions in the ECF and cytosol.
 Inability of most anions to leave the cell.
 Electrogenic nature of the Na+/K+ ATPases.
Unequal distribution of ions in the ECF
and cytosol.
 ECF is rich in Na+ and CL- ions.
 Cytosol has the cation K+ and the dominant anions are phosphates
attached to ATP and amino acids in proteins.
 The plasma membrane has more K+ leakage channels than Na+
leakage channels.
Inability of most anions to leave the
 The anions are attached to large nondiffusable molecules such as
ATP and large proteins.
Electrogenic nature of the Na+/K+
 Membrane permeability to Na+ is very low because there are
very few sodium leakage channels.
 Sodium ions do slowly diffuse into the cell, which would
eventually destroy the resting membrane potential.
 Na+/K+ ATPases pump sodium back out of the cell and bring
potassium back in.
 They pump out 3 Na+ for every 2 K+ they bring in.
Graded Potentials
 A graded potential is a small deviation from the resting
membrane potential.
 It makes the membrane either more polarized (more negative
inside) or less polarized (less negative inside).
 Most graded potentials occur in the dendrites or cell body.
Graded Potentials
 Hyperpolarizing graded potential make the membrane
more polarized (inside more negative).
 Depolarizing graded potential make the membrane less
polarized (inside less negative).
 Graded potentials occur when ligand-gated or
mechanically gated channels open or close.
 Mechanically gated and ligand-gated channels are present in
sensory neurons.
 Ligand-gated channels are present in interneurons and motor
Graded Potentials
 Graded potentials are graded because they vary in amplitude
(size) depending on the strength of the stimulus.
 The amplitude varies depending upon how many channels are
open and how long they are open.
 The opening and closing of channels produces a flow of
current that is localized.
Graded Potentials
 The charge spreads a short distance and dies out
(decremental conduction).
 The charge can become stronger and last longer by adding
with other graded potentials (Summation).
Types of Graded Potentials
 Post-synaptic potentials – a graded potential that occurs in
the dendrites or cell body of a neuron in response to a
 Receptor potentials and generator potentials – graded
potentials that occur in sensory receptors and sensory
Action Potentials
 An action potential or impulse is a sequence of events
that decrease and reverse the membrane potential and
eventually restore it to its resting state.
 Depolarizing phase – the resting membrane potential
becomes less negative, reaches zero, and then becomes
 Repolarizing phase – restores the resting membrane
potential to -70 mV.
 Threshold – depolarization reaches a certain level (about –
55 mV), voltage gated channels open.
A weak stimulus that does not bring the membrane to
threshold is called a sub-threshold stimulus.
A stimulus that is just strong enough to depolarize a
membrane is called a threshold stimulus.
Several action potentials will from in response to a suprathreshold stimulus.
Action potentials arise according to an all or none
Depolarizing Phase
 A depolarizing graded potential or some other stimulus
causes the membrane to reach threshold.
 Voltage-gated ion channels open rapidly.
 The inflow of positive Na+ ions changes the membrane
potential from –55mv to +30 mV.
 K+ channels remain largely closed.
 About 20,000 Na+ enter through the gates. Millions are
present in the surrounding fluid.
 Na+/K+ pumps bail them out.
Repolarizing Phase
 While Na+ channels are opening during depolarization, K+
channels remain largely closed.
 The closing of Na+ channels and the slow opening of K+
channels allows for repolarization.
 K+ channels allow outflow of K+ ions.
Refractory Period
 The refractory period is the period of time after an
action potential begins during which an excitable cell
cannot generate another action potential.
 Absolute refractory period – a second action potential
cannot be initiated, even with a very strong stimulus.
 Relative refractory period – an action potential can be
initiated, but only with a larger than normal stimulus.
Propagation of Nerve Impulses
 Unlike the graded potential, the impulse in the action
potential is not detrimental (it does not die out).
The impulse must travel from the trigger zone to the axon
This process is known as propagation or conduction.
The impulse spreads along the membrane.
As Na+ ions flow in, they trigger depolarization which opens
Na+ channels in adjacent segments of the membrane.
2 Types of Propagation
 Continuous Conduction – step by step depolarization and
repolarization of each segment of the plasma membrane.
 Saltatory Conduction – a special mode of action potential
propagation along myelinated axons.
 The action potential “leaps” from one Node of Ranvier to the
Continuous and Saltatory Conduction
 Few ion channels are present where there is myelin.
 Nodes of Ranvier – areas where there is no myelin – contain
many ion channels.
 The impulse “jumps” from node to node.
 This speeds up the propagation of the impulse.
 This is a more energy efficient mode of conduction.
Neurotoxins & Local Anesthetics
 Neurotoxins produce poisonous effects upon the nervous
 Local anesthetics are drugs that block pain and other somatic
 These both act by blocking the opening of voltage-gated Na+
channels and preventing propagation of nerve impulses.
Factors That Affect Speed of
 1. Amount of myelination - Myelinated axons conduct
impulses faster than unmyelinated ones.
 2. Axon diameter - Larger diameter axons propagate
impulses faster than smaller ones.
 3. Temperature – Axons propagate action potentials at lower
speeds when cooled.
Classification of Nerve Fibers
 A fibers.
 Largest diameter.
 Myelinated.
 Convey touch, pressure, position, thermal sensation.
Classification of Nerve Fibers
 B fibers.
 Smaller diameter than A fibers.
 Myelinated.
 Conduct impulses from the viscera to the brain and spinal cord
(part of the ANS).
Classification of Nerve Fibers
 C fibers.
 Smallest diameter.
 Unmyelinated.
 Conduct some sensory impulses and pain impulses from the
 Stimulate the heart, smooth muscle, and glands (part of ANS).
Encoding Intensity of a Stimulus
 A light touch feels different than a firmer touch because of
the frequency of impulses.
 The number of sensory neurons recruited (activated) also
determines the intensity of the stimulus.
Signal Transmission at Synapses
 Presynaptic neuron – the neuron sending the signal.
 Postsynaptic neuron – the neuron receiving the message.
 Axodendritic – from axon to dendrite.
 Axosomatic – from axon to soma.
 Axoaxonic – from axon to axon.
Types of Synapses
 Electrical synapse
 Chemical synapse
Electrical Synapses
 Action potentials conduct directly between adjacent cells
through gap junctions.
Electrical Synapses
 Tubular connexons act as tunnels to connect the cytosol
of the two cells.
 Advantages.
 Faster communication than a chemical synapse.
 Synchronization – they can synchronize the activity of a group
of neurons or muscle fibers. In the heart and visceral smooth
muscle this results in coordinated contraction of these muscle
Chemical Synapses
 The plasma membranes of a presynaptic and postsynaptic
neuron in a chemical synapse do not touch one another
 The space between the neurons is called a synaptic cleft
which is filled with interstitial fluid.
 A neurotransmitter must diffuse through the interstitial
fluid in the cleft and bind to receptors on the
postsynaptic neuron.
 The synaptic delay is about 0.5 msec.
Removal of Neurotransmitter
 Diffusion.
 Enzymatic degradation.
 Uptake by cells.
 Into the cells that released them (reuptake).
 Into neighboring glial cells (uptake).
Spatial and Temporal Summation of
Postsynaptic Potentials
 A typical neuron in the CNS receives input from 1000 to
10,000 synapses.
 Integration of these inputs is known as summation.
Spatial and Temporal Summation of
Postsynaptic Potentials
 Spatial summation – summation results from buildup of
neurotransmitter released by several presynaptic end bulbs.
 Temporal summation – summation results from buildup of
neurotransmitter released by a single presynaptic end bulb 2
or more times in rapid succession.
Neural Circuits
 Diverging circuit –single presynaptic neuron influences
several postsynaptic neurons (i.e. muscle fibers or gland
 Converging circuit – several presynaptic neruons influence a
single post-synaptic neuron (results in a stronger signal).
Neural Circuits
 Reverberating circuit – Branches from later neurons
stimulate earlier ones (may last for seconds to hours)
(breathing, coordinated muscular activities, waking up,
short-term memory).
 Parallel after-discharge circuit – a presynaptic neuron
stimulates a group of neurons that all interact with a
common postsynaptic cell (quick stream of impulses)
(mathematical calculations).
Neural Circuits
Neurogenesis in the CNS
 Birth of new neurons.
 From undifferentiated stem cells.
 Epidermal growth factor stimulates growth of neurons and
 Minimal new growth occurs in the CNS.
 Inhibition from glial cells.
 Myelin in the CNS.
Damage and Repair in the PNS
 Axons and dendrites may undergo repair if the cell body is
intact, if the Schwann cells are functional, and if scar tissue
does not form too quickly.
 Wallerian degeneration.
 Schwann cells adjacent to the site of injury grow torwards
one another and form a regeneration tube.