Download Curriculum vitae SRINIVAS V. RAMASWAMY 382 Finch Road

Curriculum vitae
SRINIVAS V. RAMASWAMY
382 Finch Road, Raynham, MA 02767
Email:[email protected]. Tel : (508)-821-2594
Goals: Microbiologist seeking immediate work in antimicrobial drug discovery
and development or QC/QA in the biotech/pharma industries located in MA.
WORK EXPERIENCE
Staff Scientist, 2005-2010; SelectX Pharmaceuticals, Inc., Worcester, MA
• Initiated antimicrobial screening division for drug discovery projects.
• Wrote several SOP’s for various assays and supervised technicians.
• Screen for novel chemical derivatives targeted primarily against multi-drug
resistant Pseudomonas, Klebsiella, Acinetobacter, E. coli, Staphylococcus and
other gram negative and gram positive bacteria.
• CDC, FDA, ASM, and CLSI guidelines for drug susceptibility testing.
• Drug resistance mechanisms of aminoglycosides, carbapenems and other
beta-lactams.
• Successfully identified next generation aminoglycoside for pre-clinical
development and IND studies.
• Reference: Michael Chaparian, CEO and supervisor, Tel: 508-798-0216
• Michael Brady, Group leader Tel: 508-798-0216X117
EDUCATION
Research Associate, 2003-2005; Harvard Medical School, Boston, MA
• Molecular characterization of Group B Streptococcus.
• PCR, multi-locus sequence typing (MLST), DNA sequence analysis of capsular
polysaccharide genes.
• Genetic analysis of capsular genes in clinical isolates to determine nontypable
phenotype.
• Reference: Larry Paoletti, Ph.D Tel: 617-525-2270
Post-doctoral Associate, 1997-2003; Baylor College of Medicine, Houston, TX
• Molecular epidemiology and genetic basis for drug resistance in Mycobacterium
tuberculosis and Staphylococcus aureus.
• PCR, multiplexing, microbial and human genome database mining,
spectroscopy, automated DNA sequencing and SNP analysis.
• Supervision of technicians, writing manuscripts and poster presentations.
• Reference: Ed Graviss, Ph.D, Tel: 713-798-8097
Ph.D in Microbiology, 1997, University of Oklahoma, Department of Microbiology,
Norman, OK.
• Identification and characterization of calcofluor white binding deficient mutants
in Myxococcus xanthus.
• Critically evaluate various aspects of research in microbiology and molecular
biology, teach undergraduate microbiology laboratory courses.
• Prokaryotic development, cell motility, cell-cell communication, gene
expression, microbial genetic analysis
• Molecular techniques including light and electron microscopy, DNA extraction,
restriction digestion, transformation, transduction, cloning, UV and transposon
mutagenesis, motility assays, microbial selection and screens, DNA
hybridization
• Reference: John Downard, Ph.D, Tel: 405-325-6302
Bachelor of Medicine & Bachelor of Surgery (MBBS), 1987, Mysore Medical
College, Mysore, India
• Clinical aspects of medicine and surgery
TECHNICAL SKILLS
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Clinical microbiology, staining, aseptic techniques, stock culture maintenance
Microbial identification, microbial quantitation, sterilization
Knowledgeable in bioburden, LAL (endotoxin testing), medical device, ET
Microbial selection and screens, spontaneous mutation frequency
Drug susceptibility testing (CLSI guidelines), MIC, time-kill kinetics
Synergy testing, post-antibiotic effect, drug resistance mechanisms
Light and electron microscopy, spectroscopy, plate readers
DNA extraction, restriction digestion, transformation, transduction, cloning
UV and transposon mutagenesis, PCR, multiplexing, hybridizations
DNA sequencing, SNP discovery, human & microbial genome database mining
Total protein and beta-galactosidase assays, ELISA, robotic liquid handling
Supervision of technicians, manuscript writing, poster presentations.
COMPUTER SKILLS
• PC and Macintosh computers-Word, Excel, Powerpoint, Pages, Numbers
• MacVector, Lasergene- DNASTAR, EditSeq, Megalign, NCBI resources
SELECTED PUBLICATIONS (Total published = 26)
Ramaswamy, S. V., P. Ferrieri, A. E. Flores, and L. C. Paoletti. 2006. Molecular
characterization of nontypable Group B Streptococcus. J. Clin. Microbiol.
44:2398-2403. Times cited – 14.
Biçmen, M., Z. Gülay, S. V. Ramaswamy, D. M. Musher, and D. Gür. 2006.
Analysis of mutations in the pbp genes of penicillin-nonsusceptible pneumococci
from Turkey. Clin. Microbiol. Infect. 12:150-155. Times cited – 7.
Koreen, L., S. V. Ramaswamy, S. Naidich, I. V. Koreen, G. R. Graff, E. A.
Graviss, and B. N. Kreiswirth. 2005. Comparative sequencing of the serineaspartate repeat region of clumping factor B gene (clfB) for resolution within
clonal groups of Staphylococcus aureus. J. Clin. Microbiol. 43:3985-3994. Times
cited – 10.
Shukla, S. K., M. E. Stemper, S. V. Ramaswamy, J. M. Conradt, R. Reich, E. A.
Graviss, and K. D. Reed. 2004. Molecular characteristics of nosocomial and
Native American community-associated Methicillin-resistant Staphylococcus
aureus clones from rural Wisconsin. J. Clin. Microbiol. 42:3752-3757. Times cited
– 50.
Ramaswamy, S. V., S. Dou, A. Rendon, Z. Yang, M.D. Cave, and E. A. Graviss.
2004. Genotypic analysis of multidrug-resistant Mycobacterium tuberculosis
isolates from Monterrey, Mexico. J. Med. Microbiol. 53:107-113. Times cited –
46.
Ramaswamy, S. V., R. Reich, S-J. Dou, L. Jasperse, X. Pan, A. Wanger, T.
Quitugua, and E. A. Graviss. 2003. Identification of Single Nucleotide
Polymorphisms (SNPs) in Genes Associated with Isoniazid Resistance in
Mycobacterium tuberculosis. Antimicrob. Agent Chemother. 47:1241-1250.
Times cited - 121.
Ramaswamy, S., and J. M. Musser. 1998. Molecular genetic basis of
antimicrobial agent resistance in Mycobacterium tuberculosis: 1998 update.
Tubercle Lung Dis. 79(1):3-29. Times cited - 445.
Mdluli, K., R. A. Slayden, Y. Zhu, S. Ramaswamy, X. Pan, D. Mead, D. D.
Crane, J. M. Musser, and C. E. Barry III. 1998. Inhibition of a Mycobacterium
tuberculosis β-ketoacyl ACP synthase by isoniazid. Science. 280:1607-1610.
This work was recognized by several newsletters including NIAID News,
Houston Chronicle, and Texas Medical Center News. Times cited – 285.
Ramaswamy, S., M. Dworkin, and J. Downard, 1997. Identification and
characterization of Myxococcus xanthus mutants deficient in calcofluor white
binding. J. Bacteriol. 179:2863-71. Times cited - 27.
SELECTED MEETING ABSTRACTS (Total published = 31)
Ramaswamy, S., P. Ferrieri, A. E. Flores, and L. C. Paoletti. 2005. Molecular
characterization of nontypable Group B Streptococcus. The XVIth Lancefield
International Symposium on Streptococci and Streptococcal Diseases, Cairns,
Australia.
Shukla, S. K., S. V. Ramaswamy, M. Stemper, J. Conradt,
R. Reich, E. A. Graviss, and K. D. Reed. 2004. Molecular Characterization of
Community-Associated Methicillin-Resistant Staphylococcus aureus from Native
American Communities in Rural Wisconsin. 104th ASM general meeting, New
Orleans, LA.
Ramaswamy, S. V., R. Reich, S-J. Dou, L. Jasperse, X. Pan, A. Wanger, T.
Quitugua, and E. A. Graviss. 2002. Identification of Mutations in Several New
Genes Associated with Isoniazid Resistance in Mycobacterium tuberculosis.
102rd ASM general meeting, Salt Lake City, UT.
Ramaswamy, S. V., E. A. Graviss, N. Kurepina, and B. N. Kreiswirth. 2001.
Mutations in pncA Distinguishes a Primary Spread and Acquired Resistance in
Two Multi-drug Resistant Mycobacterium tuberculosis Cohorts. Diagnostic
approaches for infectious disease: Future promises and impact on clinical
management. Meeting sponsored by IDSA, CDC, FDA, and NIAID, and held at
Orlando, FL.
Ramaswamy, S. V., A. G. Amin, S. Göksel, C. E. Stager, S-J. Dou, H-E. Sahly,
S. L. Moghazeh, B. N. Kreiswirth, and J. M. Musser. 2000. Molecular genetic
analysis of nucleotide polymorphisms associated with ethambutol resistance in
human isolates of Mycobacterium tuberculosis. 100th ASM general meeting, Los
Angeles, CA.
Ramaswamy, S., C. E. Stager, H. Soini, A. Amin, L. A. Basso, J. S. Blanchard,
S. L. Moghazeh, R. A. Slayden, C. E. Barry III, B. N. Kreiswirth, and J. M.
Musser. 1999. Comprehensive analysis of molecular genetic polymorphisms
associated with isoniazid resistance in human isolates of Mycobacterium
tuberculosis . 99th ASM general meeting, Chicago, IL.
Ramaswamy, S., and J. Downard. 1996. Identification and characterization of
calcofluor white binding deficient mutants in Myxococcus xanthus. Presented at
the 23rd annual meeting on the biology of myxobacteria, Angel fire, NM.
TEACHING EXPERIENCE
Supervised and trained technical staff at the Baylor College of Medicine
Taught the following laboratory courses at the University of Oklahoma:
M2815 - Introduction to Microbiology for non-majors
M3815 - Introduction to Microbiology for majors
M3942 - Instrumental methods in Biology Laboratory
M4845 - Pathogenic Microbiology
AWARDS
Recipient of Government of India National Merit Scholarship
RESIDENT STATUS
Citizen of the United States