Download α-Actin (Smooth Muscle) Rabbit Monoclonal Antibody Product Data

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α-Actin (Smooth Muscle) Rabbit Monoclonal Antibody
Product Data Sheet
Catalog #1184-1
Clone ID: E184
Lot #: C04018
Quantity: 100 µl
Type: Rabbit Monoclonal IgG
Species Cross-reactivity: + Human + Mouse + Rat
Applications: + WB + IHC n/d ICC + Flow Cytometry + IP
Molecular Wt.: 42 kDa
UniProt ID: P68032
Background: Actins are highly conserved proteins
ubiquitously expressed in all eukaryotic cells. They
comprise, along with microtubules, a major component of the
cytoskeleton. Actin has been found to be expressed in at
least six isomeric forms. It is expressed in heart and skeletal
striated muscle tissue, and in certain smooth muscle tissues,
regulating contractile potentials for these cells. It is also
expressed in the cytoplasm of non-muscle cells, functioning
to control cell structure and motility (1). Polymerization of
globular actin (G-actin) leads to a structural filament (F-actin),
whose formation requires the Arp2/3 complex (2). It has
been reported that actin is hyperphosphorylated in primary
breast tumors (3). It has also been shown that actin
dysfunction leads to certain types of heart failure (4).
Smooth muscle alpha actin is of further interest because it is
one of a few genes whose expression is relatively restricted
to vascular smooth muscle cells. Furthermore, expression of
smooth muscle alpha actin is regulated by hormones and cell
proliferation, and is altered by pathological conditions
including oncogenic transformation and atherosclerosis (5).
cancers: implications for adjuvant use of kinase-inhibitory
drugs. Clin. Cancer Res. 10: 3980–3987 (2004).
4. Olson, T.M., et al. Actin mutations in dilated
cardiomyopathy, a heritable form of heart failure. Science
280: 750–2 (1998).
5. Miwa, T., et al. Structure, chromosome location, and
expression of the human smooth muscle (enteric type)
gamma-actin gene: evolution of six human actin genes. Mol
Cell Biol. 11: 3296–306 (1991).
kDa
2501501007550-
Fig 1. Western blot
analysis on A431 cell
lysate using anti-α-Actin
(Smooth Muscle)
RabMAb (catalog #11841), dilution 1:50,000.
37-
Specificity: A synthetic peptide corresponding to N-terminus
of human Alpha-actin 3 was used as immunogen. This
antibody only detects actin in smooth muscle in
immunohistochemistry.
Storage Conditions: Store at -20 °C. Buffer: 50 mM TrisGlycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium
azide and 0.05% BSA. Stable for 12 months from date of
receipt.
Recommended Dilutions:
WB: 1:50,000
IHC: 1:500
Flow Cytometry: 1:20
IP: 1:20
Background References:
1. Herman, I.M. Actin isoforms. Curr. Opin. Cell Biol. 5: 48–
55 (1993).
2. Bailly, M., et al. The F-actin side binding activity of the
Arp2/3 complex is essential for actin nucleation and
lamellipod extension. Curr Biol. 11: 620–5 (2001).
3. Lim, Y.P., et al. Selective tyrosine hyperphosphorylation of
cytoskeletal and stress proteins in primary human breast
2520-
15-
A
B
Fig 2. Immunohistochemical analysis of paraffin-embedded (A)
human uterus tissue, showing positive staining of smooth
muscle, and (B) skeletal muscle tissue, showing negative
staining of skeletal muscle, using anti-α-Actin (Smooth
Muscle) RabMAb (cat. #1184-1).
Product QC’d by: __________________________________
For research use only. Not for use in diagnostic or therapeutic applications.
This product was manufactured under U.S. Patent No. 5,675,063. For a complete list of protocols and available related products, please visit www.epitomics.com.
Epitomics, Inc., 863 Mitten Road, Suite 103 Burlingame, California 94010-1303
Rev. A/04-05/JS