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35th Congress of the International Society for Oncodevelopmental Biology and Medicine (http://isobm07.vscht.cz/index.asp?m=1) Title of Symposium: Metallothioneins, analytical methods and clinical importance Chairmen of Symposium: Prof. Richard Prusa and Assoc. Prof. Rene Kizek Prof. Richard Prusa Department of Clinical Biochemistry and Pathobiochemistry 2nd Faculty of Medicine Charles University V Uvalu 84, 150 06 Prague 5 Czech Republic Phone: +420-224435300 Fax: +420-224435320, e-mail: [email protected] Assoc. Prof. Rene Kizek Department of Chemistry and Biochemistry Mendel University of Agriculture and Forestry Zemedelska 1, 613 00 Brno Czech Republic Phone: +420-545133350 Fax: +420-545212044, e-mail: [email protected] Proposed speakers and titles of presentations 1) Monica Nordberg – Biological functions of MTs in organisms Monica Nordberg: Karolinska Inst, Inst Environm Med, Stockholm, S-17177 Sweden, Karolinska Inst, Inst Environm Med, Stockholm, S-17177 Sweden, e-mail address: [email protected] 2) Paola Irato – Transcription and translation of MTs gene Paola Irato: Univ Padua, Dept Biol, Via U Bassi 58-B, Padua, I-35131 Italy Univ Padua, Dept Biol, Padua, I-35131 Italy, e-mail address: [email protected] 3) Bernd Sures – Content of MT in tissues of different organisms Bernd Sures: Universität Karlsruhe, Zoologisches Institut I, Ökologie-Parasitologie, D-76128 Karlsruhe, Germany, e-mail address: [email protected] 4) Joanna Szpunar and Biserka Raspor – Analytical techniques for determination of MT Joanna Szpunar: CNRS, UMR 5034, Equipe Chim Analyt Bioinorgan, Helioparc 2,Av Pr Angot, Pau, F-64053 France, e-mail address: [email protected] Biserka Raspor: Rudjer Boskovic Inst, Ctr Marine & Environm Res, POB 1806, Zagreb, 10002 Croatia, e-mail address: [email protected] 5) Vojtech Adam – Changes of MT level at different tumour diseases and its correlation with resistance on tumour diseases treatment Vojtech Adam: Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Zemedelska 1, 613 00 Brno, Czech Republic, e-mail address: [email protected] Abstract Metallothioneins are biologically important group of cysteine rich proteins abundant within whole animal kingdom. Nevertheless all of their biological functions are not clear yet. Thanks to their high affinity to heavy metals e.g. zinc, copper and/or cadmium, homeostatic control and detoxification of the metals are of their main known physiological function at evolutionary different animal organisms. Metals bound in a metallothionein molecule could be up to 20 % from its molecular weight. Mammalians metallothioneins have molecular weight from 6 to 7 kDa with total number of aminoacids from 60 to 68. Aromatic amino acids are not present in the MT molecule and twenty cysteines usually occur in its primary sequence at these repetitions: Cys-X-Cys, Cys-Cys-X-Cys-Cys, Cys-X-Cys-Cys, where X represents other aminoacid than cysteine. MTs consist of two binding domains (α, β) that are assembled from cysteine clusters. Cysteine sulfhydryl groups participate in covalent bindings with heavy metals. Single MT can bind up to seven divalent and/or twelve univalent metal ions. Recently, number of tumour markers has been suggested. Metallothionein could be one of the new ones. The overexpression of metallothioneins has been detected at tumour diseases. The aims of the symposium is to show and discuss i) analytical methods and techniques for analysis of metallothioneins, ii) biological function of metallothioneins at vertebrates, iii) metallothionein as a biomarker of environmental stress, iv) correlation between metallothionein levels and cancer diseases (resistance and progression of these diseases).