Download Minisympozium o metalothioneinu

35th Congress of the International Society for Oncodevelopmental Biology and Medicine
(http://isobm07.vscht.cz/index.asp?m=1)
Title of Symposium: Metallothioneins, analytical methods and clinical importance
Chairmen of Symposium: Prof. Richard Prusa and Assoc. Prof. Rene Kizek
Prof. Richard Prusa
Department of Clinical Biochemistry and Pathobiochemistry
2nd Faculty of Medicine
Charles University
V Uvalu 84, 150 06 Prague 5
Czech Republic
Phone: +420-224435300
Fax: +420-224435320, e-mail: [email protected]
Assoc. Prof. Rene Kizek
Department of Chemistry and Biochemistry
Mendel University of Agriculture and Forestry
Zemedelska 1, 613 00 Brno
Czech Republic
Phone: +420-545133350
Fax: +420-545212044, e-mail: [email protected]
Proposed speakers and titles of presentations
1) Monica Nordberg – Biological functions of MTs in organisms
Monica Nordberg: Karolinska Inst, Inst Environm Med, Stockholm, S-17177 Sweden,
Karolinska Inst, Inst Environm Med, Stockholm, S-17177 Sweden, e-mail
address: [email protected]
2) Paola Irato – Transcription and translation of MTs gene
Paola Irato: Univ Padua, Dept Biol, Via U Bassi 58-B, Padua, I-35131 Italy
Univ Padua, Dept Biol, Padua, I-35131 Italy, e-mail address: [email protected]
3) Bernd Sures – Content of MT in tissues of different organisms
Bernd Sures: Universität Karlsruhe, Zoologisches Institut I, Ökologie-Parasitologie, D-76128
Karlsruhe, Germany, e-mail address: [email protected]
4) Joanna Szpunar and Biserka Raspor – Analytical techniques for determination of MT
Joanna Szpunar: CNRS, UMR 5034, Equipe Chim Analyt Bioinorgan, Helioparc 2,Av Pr
Angot, Pau, F-64053 France, e-mail address: [email protected]
Biserka Raspor: Rudjer Boskovic Inst, Ctr Marine & Environm Res, POB 1806, Zagreb,
10002 Croatia, e-mail address: [email protected]
5) Vojtech Adam – Changes of MT level at different tumour diseases and its correlation with
resistance on tumour diseases treatment
Vojtech Adam: Department of Chemistry and Biochemistry, Mendel University of Agriculture
and Forestry, Zemedelska 1, 613 00 Brno, Czech Republic, e-mail address: [email protected]
Abstract
Metallothioneins are biologically important group of cysteine rich proteins abundant within
whole animal kingdom. Nevertheless all of their biological functions are not clear yet. Thanks
to their high affinity to heavy metals e.g. zinc, copper and/or cadmium, homeostatic control
and detoxification of the metals are of their main known physiological function at
evolutionary different animal organisms. Metals bound in a metallothionein molecule could
be up to 20 % from its molecular weight. Mammalians metallothioneins have molecular
weight from 6 to 7 kDa with total number of aminoacids from 60 to 68. Aromatic amino acids
are not present in the MT molecule and twenty cysteines usually occur in its primary sequence
at these repetitions: Cys-X-Cys, Cys-Cys-X-Cys-Cys, Cys-X-Cys-Cys, where X represents
other aminoacid than cysteine. MTs consist of two binding domains (α, β) that are assembled
from cysteine clusters. Cysteine sulfhydryl groups participate in covalent bindings with heavy
metals. Single MT can bind up to seven divalent and/or twelve univalent metal ions. Recently,
number of tumour markers has been suggested. Metallothionein could be one of the new ones.
The overexpression of metallothioneins has been detected at tumour diseases. The aims of the
symposium is to show and discuss i) analytical methods and techniques for analysis of
metallothioneins, ii) biological function of metallothioneins at vertebrates, iii) metallothionein
as a biomarker of environmental stress, iv) correlation between metallothionein levels and
cancer diseases (resistance and progression of these diseases).