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Laboratoire d’Ingénierie Moléculaire
Appliquée à l’Analyse (LIMAA, DSA)
Raphaël Gillet
Journée des Doctorants
8 novembre 2016
March 2016
1
Sylvie SCHUH - secretariat
Permanent researchers
Loïc CHARBONNIERE
Head director
PhDs
Contract researchers
Aline NONAT – CR
Câline CHRISTINE – MC
Zouhair ASFARI – IR
Alexandre LECOINTRE – AI
Raphaël GILLET – 3ème année
Joan GOETZ – 3ème année (HK)
Nabila SOURI – writing in process
Cyrille CHARPENTIER – 1ère année
Lilia TABTI – 1ère année
Soumaya KHALFALLAH – 2ème année
Alexandre DESTHEVES – AI
Anatolie GAVRILUTA – post-doc
(ICUBE)
Several master interns to come in February
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LIMAA
Molecular design
for innovative new
compounds
Applications in
detection, analysis
and imagery
Few examples :
1. Ultrabright lanthanide nanoparticles for medical imaging
2. Luminescent Ln(III) complexes: imagery, ion sensing and energy transfer
3. Bifunctional bispidine-based chelates for 64Cu, application in Positron
Emission Tomography
4. Calixarene synthesis for depollution applications
5. MIVOC
Strong interest in Lanthanides: f block atoms, 4f electrons. Why?
• Sharp fingerprint (specific wavelenghts) emission spectra
(spectral distinction)
• Long lifetime: good ratio signal/noise (time distinction)
Antenna effect. Since electron transition from f orbitals to f orbitals are unfavored, it
is necessary to develop a chromophoric system that “helps the lanthanide gather
energy” by collecting photons and transfering the energy to the lanthanide
Ln nanoparticle with ligand
at the surface
Ln complex
4
Objectives
1. Synthesis of ultrabright nanoparticles based on Terbium and Lanthanum
2. Alternatively: synthesis of functionalized chromophoric compounds for biological
targeting
3. Mixing nanoparticles and ligand: coordination at the surface
Excitation
Excitation
O
O
O
O
O
O
O
O
O
O
Emission
Excitation
O
O
Energy transfert
O
O
O
Energy transfert
O
Energy transfert
Excitation
O
Ln0,9 Tb0,1F3
O
Energy transfert
O
O
Energy transfert
O
Energy transfert
O
O
Energy transfert
O
O
Excitation
O
O
O
O
O
O
O
O
Emission
Under UV lamp
O
O
Excitation
Excitation
4. Cells test: location (lysosomes) and toxicity (non-toxic) for further applications
Fluoride-based treatment/compounds widely used: dental care, PET imaging, osteoporose
BUT
It is toxic: osteosclerosis, kidney stones for example…
Quantification is important, but the method currently used  too low sensitivity.
The idea is to design a lanthanide complex for the coordination of fluoride.
LnX3.H2O,
85 to 95%
F-
Probe OFF
Supramolecular interaction.
Probe ON
Positron Emission Tomography: based on the limited lifetime of isotopes.
Mainly used nowadays: 18F (t1/2 = 109 min)
Interesting new isotopes: 64Cu (t1/2 = 13 h). Metallic isotopes require the development
of bifunctional chelates for metal complexation and further biological interaction.
Sodium complex
O
O
O
O
C O N
O
Cs+
o
O
Depollution
o
O O
o
o
O
O
Cs+
O
O
CH3
Imagery/therapy
O
O
O
O
O
O
O
 Titane beam production to get 256Rutherfordium from 208Pb
Cp*Ti(CH3)3
MIVOC (Metal Ions from Volatile Compounds)
Thank you for your attention.
We also do that kind of stuff