Download 40837 Genetics

1. COURSE DECRIPTION – GENERAL INFORMATION
1.1. Course teacher
Mirjana Pavlica
1.2. Name of the course
Genetics
1.3. Associate teachers
1.4. Study programme (undergraduate,
graduate, integrated)
undergraduate
required
1.5. Status of the course
1.6. Year and semester of study
1.7. Credit value (ECTS)
1.8. Type of instruction (number of hours
L+S+E+e-learning)
1.9. Expected enrolment in the course
1.10. Level of use of e-learning (1, 2, 3
level), percentage of instruction in the
course on line (20% maximum)
2, 4
4
2+2+0
2-4
1
2. COURSE DESCRIPTION
2.1. Course objectives
2.2. Enrolment requirements and
required entry competences for the
course
2.3. Learning outcomes at the level of
the study programme to which the
course contributes
2.4. Expected learning outcomes at the
level of the course (4-10 learning
outcomes)
To learn and understand basic principles of genetics, laws of inheritance at the cell, individual and population level as well as
basic molecular mechanisms by which genes control growth, development and morphology.
Basic knowledge of cell biology; chemical and biological origin of life, cell structure in general, DNA structure and replication,
RNA structure and types, transcription and translation, structure and organization of cell organelles at molecular and functional
level, cell division: mitosis and meiosis
To learn and understand basic genetic principles, laws of inheritance at the cell, individual and population level and how genes
control growth and development of an organism.
Learning outcomes:
Understanding Mendelian Laws of inheritance at the cell, individual and population level
Understanding basic molecular mechanisms by which genes control growth, development and morphology of an organism
Understanding gene linkage and genetic mapping in eukaryotes, prokaryotes and bacteriophages
To learn how Mendelian inheritance differ from non-Mendelian inheritance
To learn mutations of genetic material and their effects at molecular/cell and organism level
Introducing genetic technologies: Recombinant DNA technology
Solving conceptual and experimental genetic problems
Lectures:
2.5. Course content broken down in
detail by weekly class schedule
(syllabus)
Genetics as a science; basic concepts in genetics; Mendel’s laws of inheritance; Interactions between alleles and genes
Multiple alleles (gene polymorphisms) and their inheritance; Genetics of sex; sex chromosomes; determination of sex; sexlinked inheritance in different organisms; Regulation of X-linked genes
Linkage; complete and partial; Recombination and frequency of recombination in genetic mapping
1
Gene mutations and chromosomal changes
Genetics of microorganisms and viruses; regulation of gene expression in prokaryotes
Eukaryotic genome; regulation of gene expression
Quantitative inheritance; continuing and discontinuing variability; genes in population; gene pool; gene and genotype frequency
in the population; Hardy-Weinberg equilibrium
Non-Mendelian inheritance; maternal effect; maternal inheritance; extranuclear inheritance; recombinant DNA technology;
cloning and gene manipulation
Exercises: include practical work and solving genetic problems:
Mono- and dihybrid crosses; germination and growth of F2 Arabidopsis seeds, offspring of monohybrid cross; phenotype
analysis and χ2 test
Dihybrid cross; independent assortment; germination and growth of F2 and F3 Arabidopsis seeds, offspring of dihybrid cross;
genetic ratio analysis and χ2 test
Multiple alleles; Blood group typing (ABO and Rh)
Fruit fly; growth in laboratory, male and female determination; wild type and mutants determination; crosses
Linkage; sex-linked inheritance
Chromosomal abnormalities; human karyotype; analysis of fruit fly crosses
Genetics of microorganisms; induction of lac operon by transformation of competent E. coli; induction of eukaryotic promoters
sensible to plant growth hormones – induction of expression of Arabidopsis genes sensible to auxin
Analysis of E.coli transformation; Ames-test
2.6. Type of instruction
2.8. Student responsibilities
2.9. Screening of student’s work (specify
the proportion of ECTS credits for
lectures
2.7. Comments:
independent study
seminars and workshops
multimedia and the internet
X exercises
X laboratory
online in entirety
X work with the mentor
mixed e-learning
(other)
field work
Attending work with mentor, discussing the relating topics, attending excercises, understanding and solving problems, writing
laboratory reports (exercises)
Class attendance
1
Research
Practical training
Experimental work
1
Report
2
each activity so that the total number
of CTS credits is equal to the credit
value of the course)):
2.1. Grading and evaluation of student
work over the course of instruction
and at a final exam
(Other--describe)
Seminar essay
Essay
Tests
1
Oral exam
0,5
(Other—describe)
Written exam
0,5
Project
(Other—describe)
Evaluation and grading is based on at least 4 preliminary (qualifying) exams (2 for lectures; 2 for excercises) which fullfill
requirements for the final oral exam. Successful result is at least 60% of all activities.
Title
2.2. Required literature (available at the
library and via other media)
Alberts, B., Bray, D., Levis, J., Raff, M., Roberts, K., Watson, J.D. (2008) Molecular
Biology of the Cell, Garland Publishing, New York. 5th edition
Alberts, B., Bray, D., Levis, J., Raff, M., Roberts, K., Watson, J.D. (2002) Molecular
Biology of the Cell, Garland Publishing, New York. 4th edition
Tamarin, R.H. (1999) Principles of genetics, VCB Publishers, Oxford
Brooker R.J. (2009) Genetics – Analysis&Principles, McGraw-Hill, New York
Pavlica M. (2012) Genetika – sveučilišni mrežni udžbenik;
http://www.genetika.biol.pmf.unizg.hr/
Interna skripta za praktikum: Pavlica, M. i sur. (2013) Praktikum iz genetike;
www.biol.pmf.hr (pdf materials for exersices)
2.12. Optional literature (at the time of
the submission of the study
programme proposal)
2.13. Methods of monitoring quality that
ensure acquisition of exit
competences
Qualifying writen exams
Laboratory notes after each practicum
Final oral exam
3
Number of
copies at the
library
1
Availability via
other media
3
20
2
web
web