Download Department of Chemistry and Physics

Department of Chemistry and Physics
Biochemistry/Lab
Last Date Revised: 05/25/2016
I. COURSE NUMBER AND TITLE:
CHEM 3650 EV2 Biochemistry/Lab (CRN: 20472)
 Monday, Aug. 22 – Monday, Dec. 5, 2016
 Lecture: M 6:00 PM – 8:30 PM (See location at Course Wizard)
 Lab: T 6:00 PM – 8:45 PM, Parker 113.3A
II. INSTRUCTOR:
Dr. Ketian Chen
 Email: [email protected]
 Phone: 954-262-8052
 Office Location: Parker- 383
 Office Hours: Thursday & Friday 10 AM – 12 PM, and by appointment
III. COURSE DESCRIPTION:
The chemical properties of amino acids, monosaccharides, lipids and nucleotides are discussed. The structure of
proteins, carbohydrates and biological membranes are studied. Mechanisms of enzymatic catalysis are outlined
in detail with an emphasis on the structure/function of cofactors. Glycolysis and citric acid cycle are described.
Electron transport and ATP synthesis are discussed in both mitochondria and chloroplasts. Metabolism of lipids,
amino acids and nucleotides are presented. In addition to mechanistic studies of biochemical pathways and cycles,
regulation of these processes is also covered. Prerequisites: BIOL 1500 and CHEM 2200 or CHEM 2410 or
CHEM 2410H. (Description Last Updated: Fall 2013 (201420))
IV. LEARNING OUTCOMES:
Upon the completion of the course, the student is expected to be able to:
1. Discuss the key molecular reactions involving the mechanistic studies of biochemical pathways and
processes.
2. Develop problem-solving skills, critical thinking and analytical reasoning in the field of biochemistry.
3. Demonstrate proper procedures for handling chemicals and utilizing instrumentation used in
biochemistry.
4. Develop skills to carry out biochemical protocols, collect and analyze the data.
5. Critically assess a biochemistry topic by reading relevant current research articles found and accessed
through electronic scientific databases.
V. REQURED TEXTS AND MATERIALS:
Fundamentals of Biochemistry: Life at the Molecular Level
Author: Voet and Pratt Publisher: Wiley Year: 2016 Edition: 5th
Fundamental Laboratory Approaches for Biochemistry and Biotechnology
Author: Ninfa, Ballou, and Parsons Publisher: Wiley Year: 2009 Edition:
2nd
ISBN-9781118918401
ISBN-10: 0470087668
ISBN-13: 9780470087664
VI. COURSE REQUIREMENTS AND POLICIES:
Students are expected to:
 Attend class (attendance will be recorded).
 Read assigned material before class and be prepared for class questions and discussion.
 Follow class notes, which are available online (Blackboard) and take good class notes.
 Study in groups to review the course material. This will help tremendously.
 Come prepared to perform each lab. Students attending unprepared will be dismissed earning zero credit
for that experiment. Observe all laboratory safety precautions and procedures.
 Attend lab sessions and submit timely and well-written lab reports. Every student must write their own
lab reports even though they have worked with a lab partner.
 Observe all laboratory safety precautions and procedures.
 Display college-level writing ability in written lab reports, exams and homework, in accordance with the
Writing Across the Curriculum policy.
 Contact the professor if you are having difficulties as soon as possible!
Additional Policies:
 No cell phones are allowed in class or lab. Cell phones must be turned off during class and lab.
 Recording lectures is not allowed unless you get approval from the instructor.
 It is the student responsibility to take all exams and attend all lab sessions on their scheduled dates. Failure
to do so will result in a zero for that exam or that lab session. You will not be allowed to preform lab
experiment in an alternate lab section. There will be NO makeup exams, quizzes or labs unless your
absence is due to special circumstances beyond your control (documentation in written form must be
presented either BEFORE the exam or WITHIN 24 HOURS following the exam).
 No exams or lab reports will be dropped.
 Academic dishonesty (cheating, plagiarism, etc.) on exams, homework assignments and lab reports will be
dealt with harsh penalty, at minimum, with a failing grade.
“Partners in the lab can only share the data they jointly produce, but each student needs to complete the lab
report, including data analysis and report writing, independently and individually. Producing the lab report
in any joint effort or fashion by lab partners will be considered plagiarism.”
VII. GRADING CRITERIA:
There will be three semester exams and a cumulative final exam. Additional assignments, which may come in
the form of homework, in class work, and/or discussions, will also be assigned.
Grading Scale:
A
AB+
B
B-
92.0-100
90.0-91.9
86.0-89.9
82.0-85.9
80.0-81.9
Evaluations:
C+
C
CD
F
76.0-79.9
70.0-75.9
66.6-69.9
60.0-66.5
<60.0
Exam I, II (13% each)
Midterm Exam
Final Exam
Labs (total)
Assignments
26%
13%
30%
25%
6%
VIII: COURSE SCHEDULE AND TOPIC OUTLINE: LECTURE SCHEDULE
Week
Date
Topics
Chapter
1
8/22/2016
Introduction to Amino Acids, Proteins
4, 5
2
8/29/2016
Proteins: 3-Dimensional Structure
Protein Function
6, 7
3
9/5/2016
No Class - University Holiday (Labor Day)
4
9/12/2016
Carbohydrates, Lipids and Biological Membranes
5
9/19/2016
Exam I
6
9/26/2016
Biological Membrane Transport
Enzyme Catalysis
10, 11
7
10/3/2016
Enzyme Kinetics, Biochemical Signaling
12, 13
8
10/10/2016
Mid-Term Exam
9
10/17/2016
Introduction to Metabolism, Glucose Metabolism
14, 15
10
10/24/2016
Gluconeogenesis, Glycogen Breakdown and Synthesis
Citric Acid Cycle
16, 17
11
10/31/2016
Electron Transport Chain, Photosynthesis
18, 19
12
11/7/2016
Exam II
13
11/14/2016
Lipid Metabolism
20
14
11/21/2016
Amino Acid Metabolism
21
15
11/28/2016
Mammalian Fuel Metabolism, Nucleotide Metabolism
16
12/5/2016
Final Exam (Comprehensive)
December 5, 2016, 6:00pm-8:00pm (see Course Wizard)
8, 9
22, 23
** This schedule is intended only as a guide and may change during the course of the semester.
IX: LABORATORY SCHEDULE
Week
Lab
Chapter/Exp
8/22/2016
Safety + Excel SR
Handout
8/29/2016
Internet Resources and Software for Protein Biochemistry SR
Handout
9/5/2016
Labor Day Week, No Lab
9/12/2016
Protein Purification Using Ion-Exchange Chromatography FR
Part I
Handout
9/19/2016
Protein Electrophoresis for Alkaline Phosphastase Enzyme FR
Part II (including Part I from previous lab)
Handout
9/26/2016
Protein Electrophoresis SR
Handout
10/3/2016
Enzyme kinetics of β-Galactosidase FR
Handout
10/10/2016
Midterm Exam week - No labs
10/17/2016
Enzymatic determination of glucose concentration SR
Handout
10/24/2016
Affinity Purification of His-tag Thioredoxin FR Part I
Handout
10/31/2016
Western Blot to Identify His-tag Thioredoxin FR Part II
(including Part I from previous lab)
Handout
11/7/2016
Activity Measurements of Plant Chloroplasts
Handout
11/14/2016
Measuring cellular lipid and starch content SR
Handout
** This schedule is intended only as a guide and may change during the course of the semester.
X. COLLEGE-WIDE POLICY STATEMENTS
Students should visit www.fcas.nova.edu/about/policies.cfm to access additional required college-wide
policies. It is your responsibility to access and carefully read these policies to ensure you are fully
informed. As a student in this class, you are obligated to follow these college-wide policies in addition to the
policies established by your instructor.
The following policies are described on this website:
 Academic misconduct
 Last day to withdraw
 Email policy
 Student course evaluations
 Student responsibility to register
 Student responsibility for course prerequisites
Additional Academic Resources: Nova Southeastern University offers a variety of resources that may aid
in student success. Among these resources are:
Accommodations for students with documented disabilities: For more information about ADA policy,
services, and procedures, students may call the Office of Student Disability Services at 954-262-7189 or visit
http://www.nova.edu/disabilityservices.
Tutoring and Testing Center: Free tutoring services: Students are encouraged to use the free,
individualized tutoring services offered by the Tutoring and Testing Center (TTC). TTC provides a
supportive atmosphere in which tutors and students work collaboratively on improving students’ writing, math
and/or science skills. To set up a tutoring appointment, call (954) 262-8350 or (800) 541-6682, ext. 28350 or
stop by the Tutoring and Testing Center in the Student Affairs Building, 2nd floor. For additional resources,
as well as information about tutoring at the regional campuses visit the Tutoring and Testing Center website
at http://www.nova.edu/tutoring-testing/index.html