Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Observation of plant cells I epidermal cells, guard cells, and stoma young leaf cells, cytoplasmic streaming, and plasmolysis 1. The upper epidermal cells of Zebrina pendula 1) Take out the upper epidermis and put it on the slide, then add some water, cover the coverslip. 2) Observe the shape and arrangement of cells under the lowpower, medium-power, and finally the high-power objective. 3) Observe the cell wall, nucleus, cytoplasm, and chloroplast. 4) Add a drop of Iodine stain solution to make the cell wall and nucleus more obvious. I2-KI stain 5) Draw what you see and describe the characteristics of the observed. Q: After the cells were stained, which may be seen more clearly the structure? 2. The lower epidermal cell and stoma of Zebrina pendula 氣孔(stoma) 1) Take out the lower epidermis and put it on the slide, then add some water, cover the coverslip. 下表皮細胞 (lower epidermal cells) 保衛細胞 (guard cells) 2) Observe the shape and arrangement of lower epidermal cells, guard cells, and stoma. 3) Observe the cell wall, nucleus, cytoplasm, and chloroplast. 氣孔(stoma) 下表皮細胞 (lower epidermal cells) 保衛細胞 (guard cells) 副衛細胞 (subsidiary cells) A subsidiary cell also called accessory cell that is associated with guard cells and differs morphologically from other epidermal cells. 氣孔複合物(stomatal complex) 4) Draw what you see and describe the characteristics of the observed. 3. The young leaf cells of Elodea densa (Planch.) Casp 1) Put the leaf on the slide, and drop enough water then cover the coverslip. 2) Observe the shape and arrangement of cells under 4X and 10X objective lens. 3) Observe the cell wall, nucleus and chloroplast under 40X objective lens. 4) At the same time, also observe the cytoplasmic streaming. 5) Draw what you see and describe the characteristics of the observed. Q: Are there chloroplasts in the epidermal cells, guard cells, or subsidiary cells? 細胞壁(cell wall) 葉綠體(chloroplast) 細胞質(cytoplasm) 4. Plasmolysis in Elodea densa (Planch.) Casp 1) Mount one leaf in 20% Sucrose solution on a microscope slide then cover the coverslip. 2) After several minutes the cell will have lost water, causing it to become plasmolyzed. (This process is called plasmolysis.) - Cytoplasm is the transparent structure, it is hard to observe the flow direction. - Chloroplast is one of the plant cell organelles can be observed under microscope so that the flow of chloroplast is a good indicator to observe the speed of cytoplasmic streaming. 3) Draw and describe the characteristics of plasmolyzed cells. 4) Open the coverslip and add some water. After several minutes, observe the microscope slide. Describe the changes of the observed.