Download Observation of plant cells I

Observation of plant cells I
 epidermal cells, guard cells, and stoma
 young leaf cells, cytoplasmic streaming, and plasmolysis
1. The upper epidermal cells of Zebrina
pendula
1) Take out the upper epidermis and put it on the slide, then add
some water, cover the coverslip.
2) Observe the shape and arrangement of cells under the lowpower, medium-power, and finally the high-power objective.
3) Observe the cell wall, nucleus, cytoplasm, and chloroplast.
4) Add a drop of Iodine stain
solution to make the cell
wall and nucleus more
obvious.
I2-KI stain
5) Draw what you see and describe the characteristics of the
observed.
Q: After the cells were stained, which may be seen more
clearly the structure?
2. The lower epidermal cell and stoma of
Zebrina pendula
氣孔(stoma)
1) Take out the lower epidermis and put it on the slide, then add
some water, cover the coverslip.
下表皮細胞
(lower epidermal cells)
保衛細胞
(guard cells)
2) Observe the shape and arrangement of lower epidermal cells,
guard cells, and stoma.
3) Observe the cell wall, nucleus, cytoplasm, and chloroplast.
氣孔(stoma)
下表皮細胞
(lower epidermal cells)
保衛細胞
(guard cells)
副衛細胞
(subsidiary cells)
 A subsidiary cell also called accessory cell that is associated with guard
cells and differs morphologically from other epidermal cells.
氣孔複合物(stomatal complex)
4) Draw what you see and describe the characteristics of the
observed.
3. The young leaf cells of Elodea densa (Planch.) Casp
1) Put the leaf on the slide, and drop enough water then cover the
coverslip.
2) Observe the shape and arrangement of cells under 4X and 10X
objective lens.
3) Observe the cell wall, nucleus and chloroplast under 40X
objective lens.
4) At the same time, also observe the cytoplasmic streaming.
5) Draw what you see and describe the characteristics of the
observed.
Q: Are there chloroplasts in the epidermal cells, guard cells, or
subsidiary cells?
細胞壁(cell wall)
葉綠體(chloroplast)
細胞質(cytoplasm)
4. Plasmolysis in Elodea densa (Planch.) Casp
1) Mount one leaf in 20% Sucrose solution on a microscope slide
then cover the coverslip.
2) After several minutes the cell will have lost water, causing it to
become plasmolyzed. (This process is called plasmolysis.)
- Cytoplasm is the transparent structure, it is hard to observe the
flow direction.
- Chloroplast is one of the plant cell organelles can be observed
under microscope so that the flow of chloroplast is a good
indicator to observe the speed of cytoplasmic streaming.
3) Draw and describe the characteristics
of plasmolyzed cells.
4) Open the coverslip and add some
water. After several minutes, observe
the microscope slide. Describe the
changes of the observed.