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Biology Study Guide EOC Practice Test: Go to: http://www.dpi.state.nc.us/accountability/testing/releasedforms Click on: Online EOC Biology *Please write your responses down as you complete the online test. You will be able to check it with the key once finished, but it will not save your responses for you once you exit the test. __________________________________________________________________________________________________ CHARACTERISTICS OF LIFE __________________________________________________________________________________________________ Organization: living things are made up of cells tissues organs … Reproduction: ASEXUAL: single parent cell; budding/fission, mitosis; SEXUAL: 2 parent cells; meiosis Universal Genetic Code: DNA, amino acids Growth and Development: during one’s lifetime; baby adult Metabolism: obtain and use materials and energy Stimulus and Response: respond to environment, reaction to various stimuli Homeostasis: maintain a stable internal environment Adapt and Evolve: adaptations are genetic; species evolve as a POPULATION or group __________________________________________________________________________________________________ SCIENCE LAB SKILLS __________________________________________________________________________________________________ Scientific Method: Observation Hypothesis Experiment Data Conclusion; Repeat Independent Variable: changed by the scientist -amount of water Dependent Variable: changes in response to independent variable -plant growth Control Variable: kept constant; used for comparison -plant type, soil, potting __________________________________________________________________________________________________ STRUCTURE AND FUNCTION OF LIVING ORGANISMS Bio.1.1 Understand the relationship between the structures and functions of cells and their organelles. Bio.1.2 Analyze the cell as a living system. __________________________________________________________________________________________________ Bio.1.1.1 Summarize the structure and function of organelles in eukaryotic cells (including the nucleus, plasma membrane, cell wall, mitochondria, vacuoles, chloroplasts, and ribosomes) and ways that these organelles interact with each other to perform the function of the cell. Cell: basic unit of living things Cell Theory: 1. All organisms composed of cells 2. Cell=basic unit of life 3. Cells come from other cells Plasma membrane: selectively permeable=control movement in/out; phospholipid bilayer; fluid mosaic Nucleus: control center Cytoplasm: surrounds organelles Ribosomes: protein synthesis Lysosome: cleans cell Mitochondria: makes ATP ER: lipids/proteins for transport, export Cytoskeleton: support Vacuoles: storage Cell Wall: cellulose, support/structure Golgi apparatus: modify/sort/package stuff from the ER Chloroplast: green/photosynthesis Bio.1.1.2 Compare prokaryotic and eukaryotic cells in terms of their general structures (plasma membrane and genetic material) and degree of complexity. Compound Microscope: oculars, stage, diaphragm, objectives, base, light, arm, coarse/fine adjustment Electron Microscope: see within a cell! Very advanced! Prokaryotes: no nucleus; no membrane-bound organelles; bacteria; less complex Eukaryotes: nucleus; membrane-bound organelles; more complex specialized!!; YOU are EUkaryotic! Plant cells: big vacuole, cell wall, chloroplasts = green! Animal cells: no cell wall or chloroplasts! K. Nifong 20131 Biology Study Guide Bio.1.1.3 Explain how instructions in DNA lead to cell differentiation and result in cells specialized to perform specific functions in multicellular organisms. Differentiation: undifferentiated cells become specialized when specific parts of DNA are activated; influenced by environment (all cells have same DNA and chromosomes; only certain genes are “activated” in each cell) Multicellular organisms: begin as a ball of cells; cells differentiate; cells release chemical signals to influence development/activity Bio.1.2.1 Explain how homeostasis is maintained in the cell and within an organism in various environments (including temperature and pH). Solution: one substance dissolved in another; solute and solvent: ex. salt, water pH: # that measures how acidic or basic; scale of 0-14; 7=neutral; ex. water Acid: forms Hydrogen ions in water (H+); between 0 and 7; ex. hydrochloric acid, coke Base: forms Hydroxide ions in water (OH-); between 7 and 14; ex. bleach, ammonia buffer: controls pH, minimizes change in pH Diffusion: movement from HIGH to LOW concentration Osmosis: diffusion of water; water always moves to greater solute concentration! Isotonic solution: solute concentrations are equal inside and outside: no change in cell Hypotonic solution: more solute inside than outside; water rushes in: cell swells/bursts/lyses Hypertonic solution: more solute outside than inside; water rushes out: cell shrinks/shrivels Turgor pressure: hypotonic solution; pressure of water, gives plant shape/ability to stand Plasmolysis: hypertonic; loss of pressure, cells shrink and plant wilts; animal cells shrivel Plasma membrane: phospholipid bilayer, regulates what enters/leaves cell, semi/selectively permeable membrane Carrier proteins: in plasma membrane; change shape to move molecules through Channel proteins: in plasma membrane; create opening for molecules to move through Facilitated diffusion: proteins help large molecules across membrane; no E; uses concentration gradient Passive transport: no energy or assistance required for transport across membrane; water/lipids Active transport: requires energy or assistance for transport across membrane; endo/exocytosis Bio.1.2.2 Analyze how cells grow and reproduce in terms of interphase, mitosis and cytokinesis. Surface Area: Volume: volume increases faster than cell’s surface area Effects: slows diffusion rate; rate that DNA can make enough proteins The Cell Cycle: Interphase and Mitosis; cell growth and division; I-P-M-A-T Song! Interphase: cell growth Mitosis: Prophase, Metaphase, Anaphase, Telophase; cell division Cytokinesis: after telophase; cytoplasm divides Enzymes: control cell cycle Cancer: excessive cell division Sister Chromatids: exact copy of a chromosome; separate during anaphase; connected by centromere Centrioles: structures that aid in separation of sister chromatids; “Spiderman” Spindle Fibers: microtubules branching from centrioles; pull apart chromatids; “Spiderman’s web” Bio.1.2.3 Explain how specific cell adaptations help cells survive in particular environments (focus on unicellular organisms). Contractile vacuole: contracts and removes water from the cell/organism Cilia and Flagella: structures of the cell for locomotion; cilia are hair-like and beat together; flagella=tail-like for propulsion Pseudopods: “false foot,” projection for cell that allows for movement; ex. Amoeba Eyespots: pigmented spot that detects light for the cell/organism; helps it find light; good for photosynthesis; ex. Euglena K. Nifong 20132 Biology Study Guide Taxis/Taxes: response toward or away from a stimuli Chemotaxis: response to chemicals; positive response = moves toward chem.; negative response = moves away from chem. Phototaxis: response to chemicals; positive response = moves toward light; negative response = moves away from light __________________________________________________________________________________________________ ECOSYSTEMS AND HUMAN/ENVIRONMENT INTERACTIONS Bio.2.1 Analyze the interdependence of living organisms within their environments. Bio.2.2 Understand the impact of human activities on the environment (one generation to the next). __________________________________________________________________________________________________ Bio.2.1.1 Analyze the flow of energy and cycling of matter (water, carbon, nitrogen and oxygen) through ecosystems relating the significance of each to maintaining the health and sustainability of an ecosystem. Ecological levels: Individual (species) Population Community Ecosystem Biome Biosphere Ecology: study of how organisms interact with each other and the environment Abiotic Factors: nonliving; climate, weather, soil, temperature, etc. Biotic Factors: living; plants, animals, etc. Biosphere: part of Earth that supports life; all life!!! Biome: group of ecosystems with dominant climate and communities; terrestrial and aquatic freshwater, marine (photic/aphotic), estuary, salt marsh, intertidal zone, tundra, taiga, desert, grasslands, temperate forest, tropical rainforest, tropical dry forest, savannah, etc. Ecosystem: populations interacting with each other and the environment Community: group of populations Population: group of organisms from same species living together Species: group of individuals that can successfully interbreed Habitat: where an organism lives Niche: role and position in its environment Sun: ultimate source of all ENERGY! grassmousesnakehawk Food Chain/Web: shows flow of energy through an ecosystem; arrows = direction of energy flow Trophic level: levels of food chain; energy and biomass decrease as you go up; 10% to each level ProducersPrimary consumersSecondary consumersTertiary consumers… Autotroph: makes own food; photosynthesis or chemosynthesis; producers; plants Heterotroph: consumes other organisms; consumers; omnivore, herbivore, carnivore; decomposer Carbon cycle: respiration/photosynthesis; burning fossil fuels; carbon dioxide (<1 % atmosphere) Nitrogen cycle: denitrification-N gas; nitrogen fixation-usable forms of N; lightning/bacteria Water cycle: evaporation, condensation, precipitation, transpiration (leaves of plants) Bio.2.1.2 Analyze the survival and reproductive success of organisms in terms of behavioral, structural, and reproductive adaptations. Behavior: way an organism reacts to changes Stimulus: signal that carries information; can be detected; ex. hunger or thirst Response: specific reaction to stimulus; ex. eat or drink Innate Behavior: instinct; inborn, fully functional 1st time performed; ex. babies nursing Ex. courtship, fight or flight, territoriality, aggression, circadian rhythm, migration, hibernation/estivation, parental care, pheromones/language/sound signals Learned Behavior: altered behavior as a result of experience; ex. monkeys use sticks to climb out of cage at zoo Ex. insight learning, habituation, operant and classical conditioning (Pavlov’s dog) Pavlov: food = dog salivates; food & bell = salivate; bell = salivates Adaptation: genetic trait that gives an organism an advantage in its environment K. Nifong 20133 Biology Study Guide Bio.2.1.3 Explain various ways organisms interact with each other (including predation, competition, parasitism, mutualism) and with their environments resulting in stability within ecosystems. Symbiosis: organisms live together and interact regularly with each other Commensalism – one benefits, one unaffected (+, 0); Spanish moss in trees Mutualism – both benefit (+, +); ants and acacia tree, teeth cleaner birds/alligators Parasitism – one benefits, one harmed (+, -); tick on a dog, tapeworms Exponential Growth: # of organisms increases by a constantly increasing rate; J-shaped curve R-selected: grow out of control, many offspring, short life span, small; ex. mice, weeds, bacteria Logistic Growth: growth slows or stops following a period of exponential growth; S-shaped curve K-selected: grow to K; few offsping/care, longer life span, large; ex. elephants, primates Carrying capacity: K; maximum # of organisms the habitat can support Bio.2.1.4 Explain why ecosystems can be relatively stable over hundreds or thousands of years, even though populations may fluctuate (emphasizing availability of food, availability of shelter, number of predators and disease). Limiting Factors: any factor that limits the #’s, existence, reproduction, or distribution of organisms Density-Dependent: affect large populations; disease/parasites, competition, predation, crowding/stress Density-Independent: affect all populations; abiotic factors – flood, hurricanes, drought, habitat loss Bio.2.2.1 Infer how human activities (including population growth, pollution, global warming, burning of fossil fuels, habitat destruction and introduction of nonnative species) may impact the environment. Bio.2.2.2 Explain how the use, protection and conservation of natural resources by humans impact the environment from one generation to the next. Demography: human populations; birth/death rate; fertility rate; age structure diagram; emigration/immigration Biodiversity: variety of life; 2/3 in tropical rainforest; natural resource = food, food chains, medicines Extinction: disappearance of a species; natural or human-caused; Stellar’s sea cow Endangered species: population close to extinction; Black rhinoceros Threatened species: rapid decline of population; African elephants Invasive/Exotic species: non-native species live and thrive in new habitat; ex. Cane toads! Habitat Loss: destruction of habitat; Ex. clear cutting a forest Habitat Fragmentation: breaking up a habitat; Ex. interstate cut through a forest Habitat Degradation: damage by pollution; air/water/land pollution, ozone, global warming Edge Effect: edges of habitat are affected; Ex. partially cleared forest Biological Magnification/Bioaccumulation: concentrations of a harmful substance increase at higher trophic levels; DDT Conservation: US ESA, cites, parks/rec., sustainable use, habitat corridors, reintroduction programs __________________________________________________________________________________________________ GENETICS AND EVOLUTION Bio.3.1 Explain how traits are determined by the structure and function of DNA. Bio.3.2 Understand how the environment, and/or the interaction of alleles, influences the expression of genetic traits. Bio.3.3 Understand the application of DNA technology. Bio.3.4 Explain the theory of evolution by natural selection as a mechanism for how species change over time. Bio 3.5 Analyze how classification systems are developed based upon speciation. __________________________________________________________________________________________________ Bio.3.1.1 Explain the double-stranded, complementary nature of DNA as related to its function in the cell. DNA: deoxyribonucleic acid; double helix; stays in nucleus; Adenine-Thymine, Cytosine-Guanine Replication: DNA DNA (nucleus) (DNA polymerase break H bonds; unzips; complimentary bases) RNA: ribonucleic acid; single-stranded; leaves the nucleus, Adenine-Uracil, Cytosine-Guanine mRNA = messenger; tRNA = transfer; carries amino acids; rRNA = ribosomal K. Nifong 20134 Biology Study Guide Bio.3.1.2 Explain how DNA and RNA code for proteins and determine traits. Transcription: DNA mRNA (nucleus) (DNA unzips, mRNA nucleotides match up, create single strand) Translation: mRNA proteins (ribosomes) (uses mRNA code to create proteins) Codon: 3 base sequence on mRNA; code for amino acids Anticodon: 3 base sequence on tRNA; complimentary to codon Bio.3.1.3 Explain how mutations in DNA that result from interactions with the environment (i.e. radiation and chemicals) or new combinations in existing genes lead to changes in function and phenotype. Mutations: any change in DNA sequence; frameshift –addition or deletion, point; can alter protein type/function Mutagen: anything that causes a mutation in DNA: exposure to chemicals, radiation, environmental factors Bio.3.2.1 Explain the role of meiosis in sexual reproduction and genetic variation. Meiosis: 2 divisions; produces haploid gametes; 1 diploid parent 4 haploid daughter cells; Meiosis I and II Sexual Reproduction: fusion of haploid gametes, VARIETY! Humans: 46 chromosomes; 23 pairs Homologous Chromosomes: paired chromosomes with genes for the same trait; 1 from mom and 1 from dad Crossing over: “switching” genes by homologous chromosomes; end up with new gene combinations, *PROPHASE 1 Tetrad: 4 part structure made of a pair of homologous chromosomes Aneuploidy: abnormal number of chromosomes Nondisjunction: chromosomes fail to separate correctly; Ex. Trisomy 21-Down’s Syndrome; Monosomy Polyploidy: more than 2 sets of chromosomes; common in plants Bio.3.2.2 Predict offspring ratios based on a variety of inheritance patterns (including dominance, co-dominance, incomplete dominance, multiple alleles, and sex-linked traits). Allele: particular form of a gene; one from mom, one from dad Law of Segregation: All organisms have 2 alleles for each gene Law of Independent Assortment: Alleles are inherited independently from each other; VARIETY! Homozygous: 2 of the same alleles for a trait; AA or aa Heterozygous: 2 different alleles for a trait; Aa Dominant: Aa, AA; phenotype always expressed: heterozygous and homozygous Recessive: aa; phenotype only expressed when homozygous Test Cross: determines genotypes of parents *Punnett Squares Phenotype: expression of a trait; ex: brown, tall, round Genotype: combination of alleles; ex: AA, Aa, aa Codominance: produces both phenotypes; black + white = black/white; BB+WW=BW; no recessive Incomplete dominance: produces intermediate phenotype; white + red=pink; R’R’+RR=RR’ Polygenic inheritance: 1+ genotypes code for a trait; skin color; AaBBCcDDEe… Multiple alleles: 2+ alleles exist for the trait in population; blood types: A, B, O Monohybrid Cross: a cross looking at only 1 gene or trait Dihybrid Cross: a cross looking at 2 genes or traits at the same time Gene Linkage: genes are in linkage groups; groups assort independently, not individual genes Gene Maps: map showing distances between genes K. Nifong 20135 Biology Study Guide Pedigree: family tree that shows where alleles for diseases exist circle-F, square-M, /-death, shaded-affected, half shaded-carrier Karyotype: chart of chromosome pairs Autosomes: chromosomes that resemble one another; pairs 1-22 Sex Chromosomes: 23rd pair of chromosomes; XX (female) or XY (male) Sex-linked traits: traits with alleles on sex chromosomes; usually on the X; colorblindness, hemophilia XAXA XAXa XaXa XAY XaY Dominant autosomal heredity: dominant traits on chromosome 1-22; tongue curling, Huntington’s, polydactyly Recessive autosomal heredity: recessive traits on chromosome 1-22; cystic fibrosis, Tay-sachs, PKU Bio.3.2.3 Explain how the environment can influence the expression of genetic traits. Environment: anything outside of or influencing the organism/cell Tobacco/Smoking: can contribute to lung/mouth cancer (excessive cell division) Excessive Sun Exposure: skin cancer; vitamin D from sun; sun exposure can reduce folic acid/folate Diet/exercise; genetic interaction: influences chance of diabetes/different types; heart disease risks/complications Diet: can prevent someone from developing symptoms of PKU (enzyme disorder/enzyme lacking) Bio.3.3.1 Interpret how DNA is used for comparison and identification of organisms. Gel Electrophoresis: “DNA fingerprinting,” electric current, DNA separates by size, can identify unknown PCR (Polymerase Chain Reaction): DNA strands multiplied many times; “copy machine” Restriction Enzymes: cut DNA; “scissors” in preparation for gel electrophoresis Bio.3.3.2 Summarize how transgenic organisms are engineered to benefit society. Bio.3.3.3 Evaluate some of the ethical issues surrounding the use of DNA technology (including cloning, genetically modified organisms, stem cell research, and Human Genome Project). Transgenic organism: contain inserted foreign DNA; bacteria with human insulin; can provide medicines for humans Gene Therapy: healthy foreign DNA inserted into cell with unhealthy DNA Cloning: making a copy of an individual Human Genome Project: analysis of human DNA sequence; 35-40,000 genes/chromosome Stem Cells: undifferentiated cells (embryonic or adult) that can become differentiated/specialized for intentional purposes, research, etc. Bio.3.4.1 Explain how fossil, biochemical, and anatomical evidence support the theory of evolution. Bio.3.4.2 Explain how natural selection influences the changes in species over time. Spontaneous Generation: nonliving material produces life Biogenesis: living organisms only from other living organisms Early life on Earth: anaerobic prokaryotic photosynthetic eukaryotic multicellular Lamarck: selective use or disuse of organs; lose or acquire within a lifetime; giraffe neck Darwin: naturalist; Galapagos Islands; Natural Selection – Descent with Modification; Origin of Species *Natural Selection: “survival of the fittest,” most fit survive to reproduce and pass on genes Theory of Evolution: change over time; change in existing species or appearance of new species Fossils: physical evidence for comparison of organisms through time Predator/Prey Relationships Mimicry: imitating another organism or trait for defense; mimic octopus Camouflage: blending in with environment; chameleon Structural adaptations: long necks in giraffes; webbed feet in ducks K. Nifong 20136 Biology Study Guide Homologous Structures: similar structures; different functions; same evolutionary origin; bones Analogous Structures: similar functions; different evolutionary origins; wings Vestigial Structures: no function in present organism; may have had use in ancient organism Embryology: similar as embryos; similar stages; gill slits in fish, bird, reptile, mammals Biochemistry: DNA codons universal throughout all life forms Gene Pool: sum of all alleles in a population Allele Frequency: frequency that any one allele is seen in a population Genetic Equilibrium: stable allele frequencies Hardy-Weinberg Equilibrium: in the absence of population decrease, mutation, migration, genetic drift, and nonrandom selection, allele frequencies remain constant Gene Flow: alleles brought in/out of a population due to migration; founder effect – change as a result of migration Genetic Drift: isolated chance events alter gene frequencies in a population; disrupts equilibrium Stabilizing Selection: natural selection favors the average for a trait; bell-shaped curve Disruptive Selection: natural selection favors both extreme phenotypes for a trait; m-shaped curve Directional Selection: natural selection favors one extreme phenotype for a trait; slanted bell curve Artificial Selection: selection for traits determined or selected by man; ex. dog breeding Sexual Selection: selection by one gender by another gender; ex. bird feathers, courtship behaviors Speciation: formation of a new species; successfully interbreed Geographic Isolation: separation of organisms; adapt to different environments; can no longer breed Reproductive Isolation: population can no longer breed with parent population; mating calls/courtship Temporal Isolation: species reproduce at different times; different mating seasons Punctuated Equilibrium: rapid speciation – periods of rapid change followed by period of equilibrium; Gould Gradualism: slow speciation at a consistent rate through time; Darwin Divergent Evolution: species once closely related become very different Adaptive Radiation: ancestral species evolve into several different species; “fan” Convergent Evolution: unrelated species in similar environments evolve same adaptations; cacti/deserts Coevolution: 2 species evolve in response to changes in each other over time; flowering plant/pollinator Bio.3.4.3 Explain how various disease agents (bacteria, viruses, chemicals) can influence natural selection. Bacteria: unicellular, divide asexually; can colonize quickly in organisms = sickness, disease; decreases survival chances Virus: nonliving, inject DNA into host organism for replication = sickness, disease; decreases survival chances Chemicals: exposure to certain toxins = sickness, disease; decreases survival chances Rates of natural selection: resistant viruses/bacteria exposed to chemicals can increase rate of natural selection Bio.3.5.1 Explain the historical development and changing nature of classification systems. Classification: Kingdom Phylum Class Order Family Genus Species (KPCOFGS) Humans: Animalia Chordata Mammalia Primates Hominidae Homo Sapiens Taxonomy: grouping and naming organisms; Binomial nomenclature: genus and species name; ex. Homo sapiens Evolutionary Order: anaerobic prokaryotesphotosynthetic prokaryoteseukaryotesmulticellular organisms 3 Domains: Archaea 6 Kingdoms: Archaebacteria Bacteria Eubacteria Eukarya Protists, Fungi, Plantae, Animalia Archaebacteria: prokaryotes, harsh environments: deep sea vents, hot springs Eubacteria: prokaryotes; basic bacteria, live everywhere *majority of prokaryotes Protista: eukaryotes; no organ systems; live in moist environments Fungi: eukaryotes; absorbs nutrients from environment for energy; unicellular/multicellular Plantae: eukaryotes; photosynthesis; multicellular; all plants Animalia: eukaryotes; heterotrophs; cells-tissues-organs-organ systems K. Nifong 20137 Biology Study Guide Bio.3.5.2 Analyze the classification of organisms according to their evolutionary relationships (including dichotomous keys and phylogenetic trees). Evolutionary Relationships based on: structural similarities, breeding behavior, geographical distribution, chromosomes, biochemistry Phylogeny: evolutionary history of a species; phylogenetic tree Dichotomous Key: sets of questions used to identify an organism (salamanders) Cladogram: branching diagram showing evolutionary relationships based on derived traits __________________________________________________________________________________________________ MOLECULAR BIOLOGY Bio.4.1 Understand how biological molecules are essential to the survival of living organisms. Bio 4.2 Analyze the relationships between biochemical processes and energy use in the cell. __________________________________________________________________________________________________ Bio.4.1.1 Compare the structures and functions of the major biological molecules (carbohydrates, proteins, lipids, and nucleic acids) as related to the survival of living organisms. Water: H2O, polar molecule; O-, H+; hydrogen bonds; cohesion/adhesion Organic molecules: Carbon; organic organism; Proteins, Lipids, Carbohydrates, Nucleic Acids Proteins: amino acids, peptide bond; CHONS; hair, teeth, carry O2, enzymes = “ase” Lipids: fats, oils; glycerol+3 fatty acid chains; triglycerides: saturated/unsaturated; plasma membrane phospholipids Carbohydrates: ENERGY!, sugars; mono, di, polysaccharides; starch (plants), glycogen (animals), cellulose (cell walls) Nucleic acids: DNA, RNA; made of nucleotides: sugar, phosphate, nitrogen base; code for proteins!!! Hydrogen + Oxygen Water Reactants: substances that enter a reaction Products: substances produced in a reaction Bio.4.1.2 Summarize the relationship among DNA, proteins and amino acids in carrying out the work of cells and how this is similar in all organisms. *See Bio.3.1.2 Bio.4.1.3: Explain how enzymes act as catalysts for biological reactions. Enzymes: proteins, reduce activation energy to speed up reactions; reactions with enzymes require less energy than others Lock and Key Model: enzymes are specific to their rxn., can build up or break down Denature: heat, pH change shape of enzyme; nonfunctional; cold slows enzyme function; homeostasis=important Bio.4.2.1: Analyze photosynthesis and cellular respiration in terms of how energy is stored, released, and transferred within and between these systems. PHOTOSYNTHESIS 6CO2 + 6H2O + light C6H12O6 + 6O2 Reactants = carbon dioxide, water, light Products = glucose, oxygen Light-dependent rxns = thylakoid membrane of chloroplasts in leaves of plants Light-independent rxns (Calvin Cycle) = stroma of chloroplasts in leaves of plants CELLULAR RESPIRATION ATP: Adenosine TriPhosphate; Cellular Energy! 6O2 + C6H12O6 6CO2 + 6H2O + energy Reactants = oxygen, glucose Products = carbon dioxide, water, up to 36 ATP! Glycolysis = cytoplasm, 2 ATP, anaerobic – no oxygen Aerobic: occurs in mitochondria; *AEROBIC RESPIRATION MAKES MORE ATP! Anaerobic: Fermentation: 2 ATP; Alcoholic - yeast; Lactic Acid - muscle cells; temporary! Bio.4.2.2: Explain ways that organisms use released energy for maintaining homeostasis (active transport). *See Bio.1.2.1 K. Nifong 20138