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Photosynthesis I) Energy II) Living things grow and reproduce. Biological activity requires Consumers get energy from the food they eat. Where do produces get their energy? o From the sun, however no organism can use light energy directly from the sun. Energy Conversions A) Photosynthesis CO2 + H2O + sunglight O2 + C6H12O6 Plants convert light energy from the sun into chemical energy. This energy is stored in complex sugar molecules. Energy can be used by plants or other organisms that eat them. Every animal (carnivore or herbivore) depends on plants – directly or indirectly. B) Steps of Photosynthesis 1) Absorption of light energy by a green plant ( primarily by chlorophyll – green pigment in chloroplasts) 2) Plants absorb carbon dioxide from the air and water from the soil. 3) Light energy, carbon dioxide, and water are used to make sugar. 4) Light energy is used to break down water into hydrogen and oxygen and then to combine the H with the CO2 to form sugar. 5) Oxygen is released into the air as O2 gas. 6) Some light energy absorbed by the plant is stored in the sugar molecules as chemical energy. *Small sugar molecules are linked together by chemical bonds (energy is stored in those bonds) *When sugar molecules are broken down in a cell, the energy stored is released and used to carry out cell activities. III) Electromagnetic Energy IV) The solar energy called visible light drives photosynthesis. Solar radiation is composed of electromagnetic energy that travels through space. Energy cannot be created or destroyed. When light is absorbed by a green plant, a small portion of that energy is converted into chemical energy in the process of photosynthesis. Structure of a Leaf Leaves are plant’s main photosynthetic organ. Leaf structure is closely associated with its photosynthetic function. Leaves must permit carbon dioxide access to the photosynthetic cells, but impede water from diffusing out. The oxygen waste product of photosynthesis must be allowed to escape from the leaf. Plants normally lose water from openings in their leaves called stomates. The water loss typically occurs during daylight hours when plants are exposed to the Sun. This water loss, known as transpiration, is both beneficial and harmful to plants. V) Mesophyll VI) Chloroplasts VII) Mesophyll cells are specialized for photosynthesis. These cells in the middle of the leaf contain many chloroplasts, the organelles that perform photosynthesis. Gas exchange (oxygen and carbon dioxide) takes place within the chloroplasts, and the food produced by the chloroplasts must move out of the cells to the other parts of the plant. Chloroplasts are the site of photosynthesis. These double-membrane bound organelles enclose additional membranes called thylakoids. The thylakoids are stacked to form grana, which are suspended in the stroma or the chloroplasts. Chlorophyll Chlorophyll molecules embedded in the thylakoid absorb light energy. These molecules are the most important pigments for absorbing light energy. VIII) Light Reactions and the Calvin Cycle Photosynthesis depends on an interaction between two sets of reactions and the Calvin cycle. Chlorophyll and the other molecules responsible for the light reactions are build into the thylakoid membrances. Enzymes that catalyze the Calvin cycle are located in the stroma. Beginning with the absorption of light by chlorophyll, the light reactions convert light energy into chemical energy in the form of ATP and NADPH. The ATP provides the energy, and the NADPH supplies the electrons for the Calvin cycle, which converts carbon dioxide to sugar. ADP and NADP+ result from the Calvin cycle and shuttle back to the light reactions, which regenerate ATP and NADPH. Photosynthesis Multiple Choice Questions 1. A green plant is kept in a brightly lighted area for 48 hours. What will most likely occur if the light intensity is then reduced slightly during the next 48 hours? 1. 2. 3. 4. Photosynthesis will stop completely. The rate at which nitrogen is used by the plant will increase. The rate at which oxygen is released from the plant will decrease. Glucose production inside each plant cell will increase. 2. An inorganic molecule required by green plants for the process of photosynthesis is 1. 2. 3. 4. oxygen starch carbon dioxide glucose 3. If the leaves of a geranium plant receive an adequate supply of raw materials, which graph below shows how the rate of photosynthesis is related to increasing light intensity received by the plant? 1. 2. 3. 4. Which activity occurs in the process of photosynthesis? 1. 2. 3. 4. Chemical energy from organic molecules is converted into light energy. Organic molecules are obtained from the environment. Organic molecules are converted into inorganic food molecules. Light energy is converted into the chemical energy of organic molecules. 4. 5. An organism was added to a test tube containing water, which was then sealed and placed in sunlight. The graph shows an increase in the oxygen content of the test tube over a period of time. Which type of organism was most probably added to the test tube? 1. 2. 3. 4. fresh-water animal green alga ameba virus 6. Photosynthesis is the process by which 1. 2. 3. 4. the potential energy of simple sugars is transferred to ATP molecules simple sugars are gradually broken down to form lactic acid or alcohol two simple sugar molecules combine to form maltose and water light energy is converted into the chemical energy of simple sugars 7. Most of the oxygen gas present in the atmosphere is produced as a result of 1. 2. 3. 4. photosynthesis cellular respiration dehydration synthesis decomposition 8. Which biological process is the main source of atmospheric oxygen? 1. 2. 3. 4. respiration photosynthesis hydrolysis synthesis 9. The graph represents the absorption spectrum of chlorophyll. The graph indicates that the energy used in photosynthesis is most likely obtained from which regions of the spectrum? 1. 2. 3. 4. yellow and orange red violet blue and green orange red and violet blue green and yellow 10. Eating a sweet potato provides energy for human metabolic processes. The original source of this energy is the energy 1. 2. 3. 4. in protein molecules stored within the potato from starch molecules absorbed by the potato plant made available by photosynthesis in vitamins and minerals found in the soil 11. The mass of some corn plants at the end of their growth period was 6 tons per acre. Most of this mass was produced from 1. 2. 3. 4. water and organic compounds absorbed from the soil minerals from the soil and oxygen from the air minerals and organic materials absorbed from the soil water from the soil and carbon dioxide from the air 12. The dense needles of Douglas fir trees can prevent most light from reaching the forest floor. This situation would have the most immediate effect on 1. 2. 3. 4. producers carnivores herbivores decomposers 13. The diagram represents part of a life process in a leaf chloroplast. If the process illustrated in the diagram is interrupted by a chemical at point X, there would be an immediate effect on the release of 1. 2. 3. 4. chlorophyll nitrogen carbon dioxide oxygen 14. The equation below represents a summary of a biological process. carbon dioxide + water → glucose + water + oxygen This process is completed in 1. 2. 3. 4. mitochondria ribosomes cell membranes chloroplasts 15. Which process is directly used by autotrophs to store energy in glucose? 1. 2. 3. 4. diffusion photosynthesis respiration active transport 16. Organisms that have the ability to use an atmospheric gas to produce an organic nutrient are known as 1. 2. 3. 4. herbivores decomposers carnivores autotrophs 17. Leaves of green plants contain openings known as stomates, which are opened and closed by specialized cells allowing for gas exchange between the leaf and the outside environment. Which phrase best represents the net flow of gases involved in photosynthesis into and out of the leaf through these openings on a sunny day? 1. 2. 3. 4. carbon dioxide moves in; oxygen moves out carbon dioxide and oxygen move in; ozone moves out oxygen moves in; nitrogen moves out water and ozone move in; carbon dioxide moves out 18. Which process usually uses carbon dioxide molecules? 1. 2. 3. 4. cellular respiration asexual reproduction active transport autotrophic nutrition 19. The graph below shows the results of an experiment in which a container of oxygen-using bacteria and strands of a green alga were exposed to light of different colors. Which statement best explains the results of this experiment? 1. 2. 3. 4. The rate of photosynthesis is affected by variations in the light. In all environments light is a vital resource. The activities of bacteria and algae are not related. Uneven numbers and types of species can upset ecosystem stability. 20. Which process provides the initial energy to support all the levels in the energy pyramid shown below? 1. 2. 3. 4. circulation photosynthesis active transport digestion Constructed Response Questions 1. The Control of Transpiration Plants normally lose water from openings (stomates) in their leaves. The water loss typically occurs during daylight hours when plants are exposed to the Sun. This water loss, known as transpiration, is both beneficial and harmful to plants. Scientists believe wind and high temperatures increase the rate of transpiration, but the size of each stomate opening can be regulated. Reducing the size of the openings during drought conditions may help reduce the dehydration and wilting that would otherwise occur. A leaf may lose more than its own weight in water each day. Transpiration also lowers the internal temperature of the leaf as water evaporates. On hot days, temperatures in the leaves may be from 3° to 15°C cooler than the outside air. With stomates open, vital gases may be exchanged between the leaf tissues and the outside environment. Researchers have also found many plants that use another response when leaf temperatures rise. Special molecules known as heat shock proteins are produced by plant cells and help to hold enzymes in their functional shapes. a. Identify the specific leaf structures that regulate the opening and closing of stomates. b. Identify two of the "vital gases" that are exchanged between leaf tissues and the outside environment. c. State one way transpiration is beneficial to plants. 2. In some land plants, guard cells are found only on the lower surfaces of the leaves. In some water plants, guard cells are found only on the upper surfaces of the leaves. Explain how guard cells in both land and water plants help maintain homeostasis. In your answer be sure to: • identify one function regulated by the guard cells in leaves • explain how guard cells carry out this function • give one possible evolutionary advantage of the position of the guard cells on the leaves of land plants ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ 3. Base your answer to the following question on the investigation described below and on your knowledge of biology. As part of an investigation, 10 bean seedlings in one setup were grown in the dark, while 10 seedlings in another setup were grown in sunlight. All other growth conditions were kept the same in both setups. The seedlings grown in the dark were white with long, slender stems. These seedlings soon died. The seedlings grown in the sunlight were green and healthy. a. Identify the independent variable in this investigation ____________________________________________________ The diagram below shows the setup of an experiment. b. Using one or more complete sentences, state a problem that could be investigated using this experimental setup. _____________________________________________________ _____________________________________________________ _____________________________________________________ Cellular Respiration O2 + C6H12O6 CO2 + H2O We can divide cellular respiration into three metabolic processes: glycolysis, the Krebs cycle, and oxidative phosphorylation. Each of these occurs in a specific region of the cell. 1. Glycolysis occurs in the cytosol. 2. The Krebs cycle takes place in the matrix of the mitochondria. 3. Oxidative phosphorylation via the electon transport chain is carried out on the inner mitochondrial membrane. * In the absence of oxygen, respiration consists of two metabolic pathways: glycolysis and fermentation. Both of these occur in the cytosol. I) ATP Production from Glucose In cellular respiration, the chemical energy in various nutrients, such as glucose, is transferred to ATP. In this form it can be transported to provide the energy needed to carry out metabolic functions. II) Glycolysis In glycolysis, the 6 –carbon sugar, glucose, is broken down into two molecules of a 3-carbon molecule called pyruvate. This change is accompanied by a net gain of 2 ATP molecules and 2 NADH molecules. Four ATP are made, but two are used by the process. Net gain is +2 ATP molecules. A) There are 4 important stages in glycolysis: 1) 2) 3) 4) III) Activation of glucose by ATP Splitting of glucose into two roughly equivalent phosphorylated halves Reduction of NAD+ to NADH Phosphorylation of ADP The Krebs Cycle The Kreb cycle occurs in the mitochondria matrix and generates a pool of chemical energy (ATP, NADH, and FADH2) from the oxidation of pyruvate. Pyruvated is transported into the mitochondria and loses carbon dioxide to form acetyl-CoA, and 2-carbon molecule. When acetyl-CoA is oxidized to carbon dioxide in the Krebs cycle, chemical energy is released and captured in the form of NADH, FADH2, and ATP. The Kreb Cycle produces +2 ATP molecules. IV) Oxidative Phosphorylation via the Electron Transport Chain The electron transport chain allows the release of the large amount of chemical energy stored in reduced NAD+ (NADH) and reduced FAD (FADH2). The energy released is captured in the form of ATP (3 ATP per NADH and 2 ATP per FADH2). The electron transport chain (ETC) consists of a series of molecules, mostly proteins, embedded in the inner mitochondrial membrane. The electron transport chain produces +32 ATP molecules. TOTAL ATP FROM ALL STAGES = 36 ATP V) Fermentation (no oxygen available) All cells are able to synthesize ATP via the process of glycolysis. In many cells, if oxygen is not present, pyruvate is metabolized in a process called fermentation. Fermentation complements glycolysis and makes it possible for ATP to be continually produced in the absence of oxygen. o In some plant cells and yeast o Glucose pyruvic acid ethyl alcohol + 2 ATP o Beer/wine making Lactic acid fermentation occurs in muscle cells o Glucose pyruvic acids lactic acid + 2 ATP o Muscle sores, cramps VI) Glucose and Energy The chemical energy stored in glucose generates far more ATP in aerobic respiration than in respiration without oxygen (glycolysis and fermentation). Each molecule of glucose can generate 36-38 molecules of ATP in aerobic respiration but only 2 ATP molecules in respiration without oxygen (through glycolysis and fermentation). Cellular Respiration Questions 1. Which statement best describes one of the events taking place in the chemical reaction? 1. 2. 3. 4. Energy is being stored as a result of aerobic respiration. Fermentation is taking place, resulting in the synthesis of ATP. Energy is being released for metabolic activities. Photosynthesis is taking place, resulting in the storage of energy. 2. The main result of aerobic respiration is the 1. 2. 3. 4. conversion of radiant energy into chemical energy production of lactic acid as an end product storage of energy in a polysaccharide production of ATP from the breakdown of glucose 3. Which substance is needed for aerobic cellular respiration to occur? 1. 2. 3. 4. oxygen carbon dioxide nitrogen methane 4. Which process is represented by the arrow in the diagram? 1. 2. 3. 4. growth respiration regulation excretion 5. The transfer of energy from nutrients to ATP is accomplished most directly by the process of 1. 2. 3. 4. cyclosis diffusion cellular respiration glucose synthesis 6. The diagram shows a mitochondrian. All the arrows are associated with the process of 1. 2. 3. 4. carbon fixation photochemical reaction synthesis aerobic respiration 7. The diagram shows a mitochondrian. Letter X most likely represents 1. 2. 3. 4. ATP maltose lactic acid PGAL 8. The energy found in ATP molecules synthesized in animal cells comes directly from 1. 2. 3. 4. sunlight organic molecules minerals inorganic molecules 9. In the demonstration shown in the diagram, which process performed by the peas when they start to grow causes the drop of liquid to move to the left? 1. 2. 3. 4. protein synthesis photosynthesis digestion cellular respiration 10. ATP is a compound that is synthesized when 1. 2. 3. 4. chemical bonds between carbon atoms are formed during photosynthesis energy stored in chemical bonds is released during cellular respiration energy stored in nitrogen is released, forming amino acids digestive enzymes break amino acids into smaller parts 11. In heterotrophs, energy for the life processes comes from the chemical energy stored in the bonds of 1. 2. 3. 4. water molecules oxygen molecules organic compounds inorganic compounds 12. Information concerning a metabolic activity is shown below. Substance X is most likely 1. 2. 3. 4. DNA oxygen ATP chlorophyll 13. The graphs below show the changes in the relative concentrations of two gases in the air surrounding a group of mice. Which process in the mice most likely accounts for the changes shown? 1. 2. 3. 4. active transport evaporation respiration photosynthesis 14. The production of energy-rich ATP molecules is the direct result of 1. 2. 3. 4. recycling light energy to be used in the process of photosynthesis releasing the stored energy of organic compounds by the process of respiration breaking down starch by the process of digestion copying coded information during the process of protein synthesis 15. Which statement best describes cellular respiration? 1. 2. 3. 4. It occurs in animal cells but not in plant cells. It converts energy in food into a more usable form. It uses carbon dioxide and produces oxygen. It stores energy in food molecules. 16. To remain healthy, organisms must be able to obtain materials, change the materials, move the materials around, and get rid of waste. These activities directly require 1. 2. 3. 4. energy from ATP the replication of DNA nutrients from inorganic sources manipulation of altered genes 17. Which change in a sample of pond water could indicate that heterotrophic microbes were active? 1. 2. 3. 4. increase in ozone level increase in glucose level decrease in oxygen level decrease in carbon dioxide level 18. Base your answer to the question on the diagram below and on your knowledge of biology. The diagram illustrates a process by which energy is released in organisms. Cells usually transfer the energy that is released directly to 1. 2. 3. 4. 5. glucose ATP oxygen enzymes 19. All life depends on the availability of usable energy. This energy is released when 1. 2. 3. 4. organisms convert solar energy into the chemical energy found in food molecules respiration occurs in the cells of producers and high-energy molecules enter the atmosphere cells carry out the process of respiration animal cells synthesize starch and carbon dioxide 20. The diagram below represents a biochemical process. Which molecule is represented by X? 1. 2. 3. 4. DNA starch protein ATP Short Answer Respiration Questions 1. Base your answer on the two different cells shown. Only cell A produces substance X. Both cells A and B use substance X. Identify substance X. ___________________________________________ 2. The diagram below represents a cell found in some complex organisms. The enlarged section represents an organelle, labeled X, found in this cell. Describe the function of organelle X and explain how it is important to the survival of the cell. In your answer, be sure to: identify organelle X state the process that this organelle perform identify the two raw materials that are needed for this process to occur identify one molecule produced by this organelle and explain why it is important to the organism _____________________________________________________________________________________ _____________________________________________________________________________________ _____________________________________________________________________________________ _____________________________________________________________________________________