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IDEXX IBR gE Ab Test Test With Confidence™ Differentiates between marker-vaccinated and BHV-1-infected The IDEXX IBR gE Ab Test is an enzyme immunoassay for the detection of bovine herpesvirus-1 specific gE antibodies in bovine serum, plasma and milk samples. Infectious bovine rhinotracheitis (IBR) is a highly contagious, infectious disease that is caused by bovine herpesvirus-1 (BHV-1). Typical clinical signs associated with infection include respiratory disease, but the virus can cause conjunctivitis, vulvovaginitis, abortions, encephalitis and balanoposthitis. The transition from primary manifestions of infection to a latent stage of persistency is often the source of spread after virus reactivation. Confirmation of exposure to BHV-1 is facilitated by a measurement of antibody. When used in IBR control programs together with gE-deleted vaccines, the IDEXX IBR gE Ab Test can be an effective differential test for distinguishing naturally infected animals from vaccinated animals. •Blocking ELISA to screen for infected or traditionally vaccinated cattle •Differentiation of infected animals from vaccinated animals in combination with gE-deleted marker vaccine •Serum, plasma and milk applications •6 -plate (strips) and 30-plate (solid) formats, ready-to-use reagents •Easy to automate •Compatible with xChek* software Specificity: Dutch bovine population (n=1730) Sensitivity: Temporal sera IBR gE-deleted, multivaccinated Dutch bovine Seroconversion post-IBR infection 1.4 600 Cutoff cow 2 cow 3 cow 4 1.0 400 300 0.8 0.6 200 0.4 100 0.2 0 cow 1 1.2 S/N ratio Frequency 500 0.60 0.75 0.90 1.05 1.20 0 1 3 4 5 6 7 8 9 10 11 12 13 14 Days post-infection S/N value Average S/N = 1.02; Standard deviation = 0.075 Specificity = 99.83% (95% CL 99.7–100%) Interpretation of results: S/N ratio > 0.7 negative; = 0.6–0.7 suspect; < 0.6 positive Conclusion: The IDEXX IBR gE Ab Test shows specificity greater than 99% using samples from characterized, multivaccinated herds. Conclusion: The IDEXX IBR gE Ab Test detected gE antibodies at days 9 to 10 post-infection. Source: Infectious Bovine Rhinotracheitis Virus (BHV-1) gE Antibody Test Kit, Validation studies of a new sample diluent reagent, summary report. IDEXX Laboratories, April 2001. Source: Experiences with the second generation of IDEXX IBR gE Ab ELISA test. Proceedings from: Ballagi A, Holmquist G, Palmquist S, Schroeder C, Tonelli Q, 2nd. International Symposium on BHV-1 Eradication, 1999, Stendal, Germany. 21 IDEXX IBR gE Ab Test Sensitivity Sample Characterization IBR Antibody (Ab) Status— Expected Result IDEXX IBR gE Ab Result (S/N) R1 1:64 dilution of a serum positive for Ab to IBR in a pool of negative sera ++ 0.29 (+) R2 1:2 dilution of R1 in a pool of negative sera + 0.47 (+) R3 1:2 dilution of R2 in a pool of negative sera + 0.61 (+/–) R4 Calf that received colostrums from a IBR gE-deleted vaccinated cow – 0.87 (–) R5 Calf that received colostrums from a IBR gE-deleted vaccinated cow – 0.88 (–) R6 Calf that received colostrums from a IBR gE-deleted vaccinated cow – 0.91 (–) R7 Bull, vaccinated with IBR gE-deleted vaccine – 0.86 (–) Sample R11 Reference sample from France +++ 0.05 (+) R12 Latent IBR infected cow after reactivation of infection + 0.24 (+) R14 Latent IBR infected cow after reactivation of infection + 0.22 (+) R17 IBR gE-deleted vaccinated and IBR infected calf + 0.36 (+) R18 IBR gE-deleted vaccinated and IBR infected calf + 0.46 (+) 0.30 (+) R19 IBR gE-deleted vaccinated and IBR infected calf + R20 IBR gE-deleted vaccinated and IBR infected calf + 0.48 (+) R23 IBR gE-deleted vaccinated and IBR infected calf + 0.15 (+) R31 IBR negative and nonvaccinated cow – 0.86 (–) R32 IBR negative and nonvaccinated cow – 0.86 (–) Sample R1–R32: Results of the IDEXX Reference Lab participating at the IBR Ringtrial 2001. Dr. M. Beer, Federal Research Center, Riems, Germany. – + ++ +++ ++++ negative weak positive positive strong positive very strong positive Interpretation of results: S/N value > 0.7 negative (–) = 0.6–0.7 suspect (+/–) < 0.6 positive (–) Conclusion: In this characterized sample population, the IDEXX IBR gE Ab Test showed 100% agreement with the expected results, and did not react with samples of cattle vaccinated with gE-deleted vaccine. For more information about the IDEXX IBR gE Ab Test, please contact your IDEXX representative or visit idexx.com/ibr. Corporate Headquarters IDEXX Laboratories, Inc. One IDEXX Drive Westbrook, Maine 04092 USA Tel: Fax: +1 207 556 4890 or +1 800 548 9997 +1 207 556 4826 or +1 800 328 5461 European Headquarters IDEXX Europe B.V. Scorpius 60 Building F 2132 LR Hoofddorp The Netherlands Asian Headquarters IDEXX Laboratories, Inc. 3F-5 No. 88, Rei Hu Street Nei Hu District 11494 Taipei, Taiwan Tel: +31 23 558 70 00 or +00 800 727 43399 Fax: +31 23 558 72 33 Tel: +886 2 6603 9728 Fax: +886 2 2658 8242 © 2010 IDEXX Laboratories, Inc. All rights reserved. • 09-71308-00 IDEXX, xChek and Test With Confidence are trademarks or registered trademarks of IDEXX Laboratories, Inc. or its affiliates in the United States and/or other countries. The IDEXX Privacy Policy is available at idexx.com.