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Figure 7.1 Page 111 Slide 1 1) Water is split by light energy. Oxygen escapes. Coenzymes pick up electrons, H+. Carbon dioxide, water are required Carbon dioxide, water are released 2) ATP energy drives synthesis of glucose from hydrogen and electrons, plus carbon and oxygen (from CO2). Oxygen is released 1) Glucose is degraded to CO2 and water. Coenzymes pick up electrons, hydrogen. Oxygen is required 2) Coenzymes give up electrons, hydrogen to oxygen-requiring transfer chains that release energy to drive ATP formation. ATP is available to drive cellular tasks Figure 7.2 Page 112 Slide 2 12H2O + 6CO2 Water Carbon Dioxide 6O2 + C2H12O6 + 6H2O Oxygen Glucose Water In-text figure Page 115 Slide 3 upper leaf surface photosynthetic cells (see next slide) Cutaway section of leaf Stepped Art Figure 7.3b,c Page 116 Slide 4 two outer membranes inner membrane system (thylakoids connected by channels) stroma channel stacked part of thylakoid membrane (see next slide) Stepped Art Figure 7.3d,e Page 116 Slide 5 SUNLIGHT compartment inside a thylakoid O H+ H2O H+ H+ H+ e– NADP+ NADPH H+ ATP ADP + Pi H+ Light-Independent Reactions CO2 H2O P glucose carbohydrate end product (e.g., sucrose, starch, cellulose) Figure 7.3f Page 117 Slide 6 Reactants Products 12H2O 6O2 6CO2 C6H12O6 6H2O Stepped Art In-text figure Page 116 Slide 7 sunlight water uptake carbon dioxide uptake ATP LIGHT DEPENDENT REACTIONS ADP + Pi NADPH LIGHT INDEPENDENT REACTIONS NAD+ P oxygen release glucose new water In-text figure Page 117 Slide 8 energy input from sun Photoautotrophs (plants, other producers) nutrient cycling Heterotrophs (consumers, decomposers) energy output (mainly heat) Figure 7.4 Page 118 Slide 9 Low energy wavelength High energy wavelength In-text figure Page 118 Slide 10 Wavelength of light (nanometers) Figure 7.5a Page 118 Slide 11 chlorophyll b wavelengths (nanometers) percent of wavelengths absorbed percent of wavelengths absorbed chlorophyll a beta-carotene phycoerythrin (a phycobilin) wavelengths (nanometers) Figure 7.6a,b Page 119 Slide 12 (combined absorption efficiency across entire visible spectrum) chlorophyll b chlorophyll a chlorophyll a carotenoids chlorophyll b phycoerythrin phycoerythrin (a phycobilin) (a phycobilin) Figure 7.6c Page 119 Slide 13 Chlorophyll a Beta-carotene Figure 7.7 Page 120 Slide 14 water-splitting complex H2O thylakoid compartment 2H + 1/2O2 P680 acceptor PHOTOSYSTEM II (light green) P700 pool of electron carriers acceptor stroma PHOTOSYSTEM I (light green) Figure 7.10 Page 121 Slide 15 incoming light reaction center PHOTOSYSTEM Figure 7.11 Page 122 Slide 16 electron acceptor e– electron transfer chain e– e– e– ATP Electron flow through transfer chain sets up conditions for ATP formation at other membrane sites. Figure 7.12 Page 122 Slide 17 sunlight THYLAKOID COMPARTMENT H2O photolysis second electron transfer chain e– e– first electron transfer chain PHOTOSYSTEM II NADP+ PHOTOSYSTEM I ATP SYNTHASE NADPH ADP + Pi ATP STROMA Figure 7.13a Page 123 Slide 18 Potential to transfer energy (voids) second transfer chain e– first transfer chain e– e– NADPH e– (Photosystem I) (Photosystem II) H2O 1/2 O2 + 2H+ Figure 7.13b Page 123 Slide 19 Photolysis in the thylakoid compartment splits water H2O e– H+ is shunted across membrane by some components of the first electron transfer chain Gradients propel H+ through ATP synthases; ATP forms by phosphate-group transfer acceptor ATP SYNTHASE PHOTOSYSTEM II ADP + Pi ATP Stepped Art Figure 7.15 Page 124 Slide 20 6 CO2 CARBON FIXATION 6 unstable intermediate 6 RuBP 12 PGA 6 ADP CALVINBENSON CYCLE 6 ATP 12 ATP 12 NADPH 4 Pi 12 ADP 12 Pi 12 NADP+ 10 PGAL 12 PGAL 2 Pi PP glucose PGAL Stepped Art Figure 7.16 Page 125 Slide 21 Leaf cross-section from C3 plant upper epidermis palisade mesophyll spongy mesophyll lower epidermis air space stoma vein Do not post on Internet Figure 7.17a Page 126 Slide 22 Stomata closed: CO2 can’t get in; O2 can’t get out X Rubisco binds oxygen, not carbon dioxide RUBP 6 PGA + 6 glycolate Calvin-Benson Cycle 5 PGAL 6 PGAL 1 PGAL 6 CO2 + water Twelve turns of the cycle required to make one 8-carbon sugar Photorespiration in a C3 plant Figure 7.18a Page 127 Slide 23 Leaf cross-section from C4 plant upper epidermis mesophyll bundle-sheath cell lower epidermis vein stoma (with air space above) Do not post on Internet Figure 7.17b Page 126 Slide 24 Stomata closed: CO2 can’t get in; O2 can’t get out C4 carbon fixation X mesophyll cell PEP oxaloacetate C4 cycle malate bundle-sheath cell RuBP pyruvate CO2 Calvin-Benson Cycle 12 PGA 10 PGAL 2 PGAL 1 sugar 12 PGAL Figure 7.18b Page 127 Slide 25 CO2 uptake at night only C4 cycle operates at night when stomata are open CO2 that accumulated during night is used during day for C3 cycle in same cell C4 cycle Calvin-Benson Cycle 1 sugar CAM plant Figure 7.19 Page 127 Slide 26