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Acrodisc® WBC (White Blood Cell) Syringe Filter High Efficiency Leukocyte Capture and Recovery The Acrodisc WBC premium syringe filter is ideal for use in the capture and recovery of leukocytes and the separation of red blood cells. Acrodisc WBC provides a simple and efficient method to isolate blood cells used in a variety of research applications including: Antigen specificity and modulation studies Immune receptor analysis Receptor protein studies Genotyping Gene expression Cell therapy Reduced Sample Volume and Processing Time Ordering Information Pall Life Sciences’ Acrodisc Premium Syringe Filter (PSF) is a proven sample prep device for the laboratory. The white blood cell (WBC) isolation syringe filter contains Pall’s patented Leukosorb® media, a fibrous matrix designed for use in procedures requiring the capture and recovery of leukocytes or the separation of erythrocytes (red blood cells) and platelets from whole blood samples. VWR Cat. No. Pall Part No. Description Pkg CA10127-206 AP-4851 25 mm 25/pkg Quick and easy method for capturing and harvesting leukocytes for use in nucleic acid isolation or other analyses Allows red blood cells and platelets to easily pass through membrane Simple and easy handling using standard luer-lock connector Efficiency 4 10 3 10 2 10 1 10 0 SSC-H: Side Scatter 10 0 200 400 600 800 1000 FSC-H: Forward Scatter Ungated post big LR filter PI Event Count: 36266 10 4 10 3 10 2 10 1 10 0 10 10 4 10 3 10 2 10 1 10 0 1 10 2 10 3 Ungated post big LR filter PI Event Count: 36266 11.6 0 10 1 10 2 10 3 10 4 Post-Filter Acrodisc WBC 4 10 0.0386 10 Nucleated WBCs Ungated litter LR, PI Event Count: 59683 Traditional filter 0 Unfiltered Sample 10 SSC-H: Side Scatter Ease of Use SSC-H: Side Scatter The Acrodisc PSF WBC contains Pall’s effective filtration area (EFA) that allows for faster filtration and separation of the sample, resulting in very low hold up volumes. The combination of the EFA in conjunction with the capacity of the Leukosorb media to efficiently separate blood cells results in higher cell recoveries from smaller sample volumes. Performance SSC-H: Side Scatter The filter removes >90% of the leukocytes from whole blood samples allowing collection of a greater population of enriched red blood cells (RBCs) from the sample. The RBCs can be used in a variety of cellular and antigenic analyses. The removed leukocytes are efficiently captured and recovered for use in further analysis in various immune or gene expression studies. Acrodisc PSF WBC Syringe Filter with Leukosorb Membrane 10 4 10 3 10 2 10 1 10 0 1.42e-3 10 0 10 1 10 2 10 3 10 4 Ungated litter LR filter PI Event Count: 281252 Propridium idodide More efficient white blood cell separation as compared to Buffy Coat density gradient methods Flow cytometry data showing Leukocyte retention effectiveness for the Acrodisc WBC Yields greater cell capture and recovery with less sample compared to larger filter format Results in very low hold up volume compared to larger white blood cell isolation filters (Hold up Volume < 2 mL, with total sample volume = 12 mL) Ordering: ca.vwr.com Technical Assistance: [email protected] Product Support: www.pall.com/lab © 2015, Pall Corporation. Pall, , Acrodisc, and Leukosorb are trademarks of Pall Corporation. ® indicates a trademark registered in the USA. Filtration.Separation.Solution.SM is a service mark of Pall Corporation. 4/15, PDF, GN14.0711CA 0115 Lit. No. 161135WCN.CA