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ANIMAL AND PLANT PHYSIOLOGY 2010-11 Teachers: Animal Physiology (6 CFU) Dott. Giovannella Bruscalupi, Dipartimento di Biologia Cellulare e dello Sviluppo (ex Istituto Fisiologia Generale), II floor, tel. 39 6 49912306. e-mail: [email protected] Plant Physiology (3 CFU) Prof. Felice Cervone, Dipartimento di Biologia Vegetale, II floor, tel: 39 6 49912517, e-mail: [email protected]. Target students: Environmental Sciences 3rd year Level: introductive Pre-requisites: knowledge of mathematics, physics, inorganic and organic chemistry and cell biology. Credits: 9 (6 Animal Physiolgy + 3 Plant Physiology) SSD: BIO-09 e BIO-04 Content of the course ANIMAL PHYSIOLOGY The animal cell. Chemical composition, structure and function of carbohydrates, lipids and proteins. Function of subcellular organelles. Cellular energetics. Plasma membrane: structure, specialized domains. Transport across membranes: simple diffusion, facilitated diffusion, active transport. Endocytosis and exocytosis. Trans-epithelial transport. Surface receptors. Electrophysiology Electric properties of the plasma membrane. Ionic basis of membrane potential. The action potential and conductance of electric impulses. Nerve cell organization. Synapses and impulse transmission. Spinal and autonomous neural circuits. Sensory transduction. Physiology of movement. Skeletal muscle: excitability, molecular basis of muscle contraction, excitation – contraction coupling. Smooth muscle. Cardiovascular system. Electrical activity of the heart, contractility, excitability, refractory period. Regulation of cardiac output. Circulatory system: hemodynamics, arteries, capillaries, veins, flux and pressure, transcapillary exchange. Arterial pressure regulation. Respiratory system. Mechanical aspects. Blood gas transport and tissue gas exchange. Central organization of breathing. Mechanical and chemical control of breathing. The internal fluids. Water body content and compartimentation. Plasma proteins. Blood pH. Osmotic regulation. Renal function. Functional anatomy of th kidney. Ultrastructure and function of the nephron: glomerular filtration, tubular reabsorption and secretion. Medullary osmotic gradient. Urine concentration. Ionic and volume regulation. Nutrition, digestion and absorption. Energy balance. Carbohydrate, lipid and protein digestion. Gastrointestinal secretions: acid, bases and digestive enzymes. Absorption in the intestinal mucosa. Hormonal control. General principles of endocrine physiology. Chemical classification of hormones. Regulation of hormone secretion. Hypothalamus and pituitary gland. Mechanism of action of peptidic and steroid hormones. Surface and intracellular receptors. Signal transduction: second messengers. Glycemia regulation. Reproduction regulation. PLANT PHYSIOLOGY Functional peculiarity of the plant cell. Vacuoles. Peroxysomes. Protein bodies. Oleosomes. Plastids. Plant cell wall. Composition, structure and biogenesis. Plasmodesms: structure and function. Electrochemical potential and water potential. Experimental methods for measurement of water potential. The movement/flow of water in the plant. Long-distance transport. Radical pressure. Cohesion-tension model. Soil-plant-atmosphere continuum. Water soilroot transport. Water transport to leaves. Transpiration and its regulation. The stomes: control of opening and closing. Phloem translocation. Pressure-flow model of phloem transport. Ionic pumps. Physiological meaning of the membrane electric potential. Calcium: compartimentalisation, accumulation and mobilization. Photosynthesis. Photosynthetic pigments. Spatial organization and function of the photosystems. Non cyclic transport of electrons, donor and acceptors of electrons. Proton transport and photophosphorylation. Cyclic and pseudocyclic transport. Tylacoid membranes. Photoinhibition. Calvin cycle and its regulation. Photorespiration. CO2 concentration systems: C4 plants. CAM acid metabolism. Skills to develop and learning outcomes expected Understanding the mechanisms underlying the functioning of cells and organisms using experimental data. Analysis of correlations between structure and function. Understanding the basic mechanisms of homeostatic control and adaptation in cells and organisms. Ability to execute, analyze and discuss reports and perform simple laboratory experiments. CONTENT (CFU) 5 CFU Diffusion, osmosis, enzymes (1 CFU ) Fisiologia Vegetale (3 CFU) Lectures Lab exercises Lectures Classroom hours 40 Home study hours 85 Total hours per student 125 12 13 25 24 50 74 Check of the results Final written test and optional ongoing tests Written reports and oral discussion Final written test and optional ongoing tests The evaluation will take place at the end of teaching with an oral exam on the whole program, taking into account the results of two ongoing optional trials. Recommended texts : Randall Eckert : Fisiologia animale - Zanichelli Richard D. : Fisiologia degli animali - Zanichelli Poli A : Fisiologia degli animali . Regolazione Diversità Adattamento - Zanichelli Hill R :Fisiologia animale - Zanichelli Sherwood L: Fisiologia degli animali . Dai geni agli organismi - Zanichelli Berne e Levy: Fisiologia - Casa Editrice Ambrosiana Silverthorn : Fisiologia una visione integrata – Casa Editrice Ambrosiana F. Cervone, P.Pupillo, C. Cresti : La Biologia Vegetale, 2003, Zanichelli