Download Bioengineering Teaching Lab Set-up

Bioengineering Teaching Lab Set-up
By
Dr. Mirjana Pavlovic
Why: in general?
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As continuation/extension of 12 years development of MS Bioengineering
program
Frequent critique/demand in SPOTs (student request)
Already developing Research Bioengineering Labs
Development of undergraduate Bioengineering program
Member of Steering Committee for development of that program
Dissertation Supervisory Committee Member for three Graduate Students,
which will be acting in support to train other students taking the course in
hands-on.
So, the money invested by students will come back to them as the
investment in teaching and elevation of their practical knowledge.
What will new Technologies bring?
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The highest possible and almost perfect merge of life
science/medicine and engineering (electrical, mechanical,
optical, etc)
Learning about them and how to use them, students will highly
benefit through conceptualization of their own innovations.
I believe it is our collective responsibility as a College to provide
an avenue to better train and develop our students to make
them competitive with students in other schools in United States
and parts of the World,
especially in this case that the students are actually the
ones demanding for such an opportunity.
The ultimate goal?
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In summary, we expect that offering these skills early will
allow students to gain experience in their field early so
they become better optimal candidates when applying
for jobs or into top-tier graduate programs.
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That is what this teaching lab would have as the ultimate
beneficial effect/goal.
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Number of student
On average basis there was about 20
students in groups 1, 2, 3 per
semester (~60 per year), 6 in DIS
group, and 30 in Summer Campus
for High School training. Without
undergraduate group which is to be
started some time it is about 300
students within 3 years.
As for faculties, it will be me and at
least 6 young faculties that are
developing their research labs, now
(7). They will also be involved in
teaching.
Colleges and students affected
A. Particularly Bioengineering Courses such as:
1. Introduction to Bioengineering (core): BME 5000
2. Tissue Engineering: BME 6334
3. Stem Cell Engineering: BME 6324
4. DIS Non-Thesis Masters : BME 6905
5. Summer campus High School young Talents: EGN 1935-003_2016R_61412
B. College of Engineering and Computer Science
1. EEL and Computer Science
2. Mechanical Engineering
3. Ocean Engineering
C. College of Science
1. Biochemistry
2. Biology
D. College of Medicine
Summary
Setting up this lab will also reinforce and capture 3 of the 4 research
advancement and strategic plans for the period between 2015 –
2025 mentioned by President Kelly’s during the “State of the
Campus” address last year:
Healthy ageing (Stem and cancer cell research);
Neuroscience (Molecular and Cellular aspect);
Ocean Science (Marine drugs testing and effects).
SENSORS?
Countess II FL Automated Cell Counter
Website Link:
https://www.thermofisher.co
m/us/en/home/lifescience/cell-analysis/cellanalysisinstruments/automated-cellcounters/countess-ii-flautomated-cell-counter.html
Video Link:
https://www.youtube.com/w
atch?v=kLmL1172zgY
Zoe™ Fluorescent Cell Imager
Website Link:
http://www.bio-rad.com/enus/product/zoe-fluorescentcell-imager
Video Link:
https://www.youtube.com/w
atch?v=KwVBgUgSX7c
XFp Extracellular Flux Analyzer
Website Link:
http://www.agilent.com/enus/products/cell-analysis(seahorse)/seahorseanalyzers/seahorse-xfp-analyzer
Video Link:
http://www.agilent.com/enus/products/cell-analysis(seahorse)/energy-pathways
Magnetic Bead Cell Sorter (MACS)
Website:
http://www.miltenyibiotec.com/en/
products-and-services/macs-cellseparation/macstechnology/microbeads_dp.aspx?
exportPF=1 Video Link:
https://www.youtube.com/watch?v
=JQQlXL0O4uw
Budget
ITEM
COMPANY
QUANTITY
COST
Zoe Fluorescent Cell
Imager
Bio-Rad Technologies
1
$ 7,495
Countess II FL Cell
Counter
Thermofisher Scientific
Magnetic Beads Cell
Sorter (MACS)
Miltenyl Biotec
XFp Extracellular Flux
Analyzer
(Agilent Technologies
(Seahorse Biosciences)
Total
(Promo Code: ZOE4LESSUS)
1
(Plus consumables & Evos
Fluorescent light cubes)
1
(Mini & MidiMACS Starting
Kits)
1
(Plus Consumable & Kits)
$ 12,456.10
$2080
$ 58, 000
$80,031
(w/o XFp = $ 22,031)
Dimensions
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DIMENSIONS AND WEIGHT OF INSTRUMENTS
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1. Zoe Fluorescent Cell Imager:
Catalog # = 145-0031 (Bio-Rad)
Instrument Dimensions (W x D x H) = 33 x 32 x 30 cm (13 x 12.6 x 11.8 inches)
Instrument Weight = 9 kg (19.8 lb)
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2. Countess II FL Cell Counter:
Catalog #: AMQAF1000 (Thermofisher Scientific)
Instrument Dimensions (W x D x H) = 228.6 x 139.7 x 228.6 mm (9 x 5½ x 9 inches)
Instrument Weight = 8 lb
3. XFp Metabolism Analyzer:
Catalog #: S7802A (Seahorse Bioscience)
Instrument Dimensions (W x D x H) = 43.0cm x 30.3cm x 56.8cm (16.9˝ x 11.9˝ x 22.4˝)
Instrument Weight = 14.7 kg (32.5 lb)
4. Magnetic Microbeads MACS (MiniMACS & MidiMACS Starting Kits)
Cat #: 130-091-632 (Miltenyl Biotec)
Weight of the MiniMACS Separator = 80 g
Weight of the MidiMACS Separator = 300 g
Dimension (Size) of the MultiStand (W × D × H) = 240 × 205 × 210 mm
Weight of the MultiStand = 2.15 kg
RESEARCH PROJECT BEHIND- GENERAL IDEA:
TO DEVELOP BIOMAGNETISM RESEARCH AT FAU
We are working on the project that we could tentatively entitle: Physical methods for targeting Cancer Stem Cells.
This is a new and evolving field of research within which we want also to see if the leading theories of Cancer (Cancer Stem Cell
Theories and Metabolic Theory of the cancer) work. We shall have the entire range of electromagnetic irradiation, but at this point we
are treating them with low frequencies. It is confirmed through some initial experiments that low frequency irradiation can cause
targeted death of cancer stem cells by disrupting their membranes.
Our hypothesis is that physical, targeted cancer stem cell therapy is possible due to specificity of resonance between particular
electromagnetic field and magnetic field around cancer stem cell membrane which creates distension in the membrane of this cell as
underlying mechanism of its death. This implicates the differences in the structure of normal cell, cancer stem cell and other cells in
the tumor which are considered non-tumorigenic.
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According to new theories of cancer stem cells, beside striking functionality of tumorigenesis and metastatic capacity, cancer
stem cells have specific extra or intra-cellular markers which are clonally (clonal mechanism theory) or mutationally (hierarchical
theory) perpetuated through the cell without any changes. Within last decade many of them are found and helped the
development of the cancer stem cell origin of the tumor (Dick, 1997, AL-Hajj,2004, Li, 2007 e.t.c.).
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This concept has also assisted in development of Targeted Cancer Stem Cell Therapy. Therefore, we are using Prostate Cancer
Stem cell as the subject of low frequency targeted electromagnetic treatment in expectation to get detrimental cellular effect. This
model has a CD44+ marker for cancer stem cell tumorigenicity. (Functionality and phenotype have to work together.).
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1.
Saheed's abstract: Cytostatic and Metabolic Effects of Electromagnetic Field Therapy on Prostate Spheroid Forming Cells
(Prostate Cancer Stem/Cells).
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2.
Evans abstract: Sequencing of specific marker from isolated prostate CSCs
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3. Mazhar's abstract: Adaptive Role of weak magnetic fields in Modulating Metabolic Pathways in Spheroid forming Prostate
Cancer Cells/Prostate Cancer Stem Cells.