Download Biosketch - NC State University

Klaenhammer, Todd R.
BIOGRAPHICAL SKETCH
Provide the following information for the key personnel in the order listed on Form Page 2.
Follow this format for each person. DO NOT EXCEED FOUR PAGES.
NAME
POSITION TITLE
Todd R. Klaenhammer
Distinguished University Professor of Food Science,
Microbiology, and Genetics
EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.)
INSTITUTION AND LOCATION
DEGREE
(if applicable)
YEAR(s)
BS
MS
MS
Ph.D.
PhD
1973
1975
1975
1978
1978
University of Minnesota, Minneapolis, MN
University
Paul, MN
MN
University of
of Minnesota,
Minnesota, St.
St. Paul,
University
University of
of Minnesota,
Minnesota, St.
St. Paul,
Paul, MN
MN
FIELD OF STUDY/Minor
Microbiology
Food
Food Science/Biochemistry
Science/Biochem.
Food
Food Science/Genetics
Science/Genetics
Academic and Professional Appointments
Assistant Professor, Departments of Food Science & Microbiology, 78-83
Associate Professor, Departments of Food Science & Microbiology, 83-88
Technical Director of the Southeast Dairy Foods Research Center, 88-92
Professor, Departments of Food Science & Microbiology, 88-92
Professor Associe in Sciences et Technologie de Aliments, University of Laval, Canada, 93-94
NCSU Alumni Distinguished Professor, 93 & 94
Director of the Southeast Dairy Foods Research Center, 93 - present
William Neal Reynolds Professor, College of Agriculture and Life Sciences, 92 –present
Distinguished University Professor, 2002 - present
SELECTED PROFESSIONAL SERVICE ACTIVITIES
1984-1986
Pfizer Award Selection Committee, American Dairy Science Association
1987
Chairman, Pfizer Award Selection Committee, American Dairy Science Association
1985-present
USA National Committee of the International Dairy Federation, Group of experts
1989
ESCOP Strategic Planning Workshop, Processing and Value Added Subcommittee.
1992-1993
Executive Committee, IFT Biotechnology Section
1996-1998
Samual Cate Prescott Award Committee
1992-94, 1997-99
Borden Award Selection Committee, American Dairy Science Association
1994-1998
American Dairy Science Association Research Committee
1999
National Academy of Sciences, National Research Council, Committee on National Security Implications of
Advances in Biotechnology: Threats to Plants and Animals
2000
IFT Task Force on Food Biotechnology: Expert Panel
2000 – 2007
Science Advisor, Einstein Institute for Science, Health and the Courts and ASTAR, in Food/Agricultural
Biotechnology
2003-2009
Committee on Scientific Programs, National Academy of Sciences
2003-2009
German/US Frontiers of Science, National Academy of Sciences Representative on Organizing Committees
2004-2006
IFT Marcel Loncin Research Prize Jury
2008-present
Scientific Advisory Board, Alimentary Pharmabiotic Center, UCC, Ireland
2007-present
Scientific Advisory Board for National Academy of Finland Centre of Excellence in Microbial Food Safety
2009
Editoral Board, Molecular Biotechnology
2007-2010
Sigma Xi Distinguished Lecturer
1980-present
Yearly add hoc reviewer for USDA/National Research Initiative, SBIR, and NIH grants.
Awards and Honors
Pfizer Award, American Dairy Science Association , 1984
Sigma Xi Research Award. NC State University , 1986
Certificate of Distinguished Scholarly Achievement. NC State University, 1986
Nordica International Research Award. American Cultured Dairy Products Institute, 1989
Samuel Cate Prescott Research Award. Institute of Food Technologists, 1989
PHS 398/2590 (Rev. 05/01)
Page
Biographical Sketch Format Page
Principal Investigator/Program Director (Last, First, Middle):
Alumni University Research Award. North Carolina State Univ. Alumni Association, 1990
Dairy Research Foundation Award. American Dairy Science Association, 1990
Gamma Sigma Delta Certificate of Merit, NCSU, 1995
Borden Award. American Dairy Science Association, 1996
Research and Development Award Institute of Food Technologists, 1998;
Fellow, Institute of Food Technologists 2000;
Fellow, American Academy of Microbiology - 2001
Outstanding Achievement Award of the University of Minnesota, June 7, 2001
Elected member in the National Academy of Sciences, May 1, 2001.
Distinguished University Professorship, November 1, 2001, NC State University
William C. Frazier Memorial Award. May 29, 2002. The Department of Bacteriology, Food Science, Food Microbiology and
Toxicology, Food Research Institute, the University of Wisconsin, Madison.
William C. Haines Dairy Science Research Award, 2006
Alexander Quarles Holladay Medal of Excellence, North Carolina State University, 2007
Nicolas Appert Award, Institute of Food Technology, 2007
Julius Bauermann Lectureship Award, Philadelphia Section of the Institute of Food Technologists, 2008
PROGRAM DESCRIPTION:
Our program employs genetic approaches for the improvement and diversification of lactic acid bacteria that are used as starter
cultures in food/dairy fermentations and as probiotics. Specific emphasis has been focused on investigating the molecular
mechanisms through which bacteria protect themselves against bacteriophage attack and developing novel mechanisms that
circumvent phage infection, prevent proliferation, or close genetic routes for phage evolution. Recent efforts in genomics are
investigating the molecular mechanisms responsible for the survival and activity of probiotic Lactobacillus species in the
gastrointestinal tract and use of this information to develop live bacterial delivery systems for bioactive compounds in vivo
(e.g. vaccines, enzymes). Teaching interests include Food and Dairy Microbiology, Fermentation Bioprocessing, and Food
Biotechnology with special emphasis on the application of fundamental molecular biology to practical issues facing the developing
food and dairy industries.
Publications
Sturino, J.M. and T.R. Klaenhammer. 2004. Antisense RNA targeting primase interferes with bacteriophage replication in
Streptococcus thermophilus. Appl. Environ. Microbiol. 70:1735-1743.
Azcarate-Peril, M.A., E. Altermann, R.L. Hoover-Fitzula, R.J. Cano, and T.R. Klaenhammer. 2004. Identification and inactivation of
genetic loci involved with Lactobacillus acidophilus acid tolerance. Appl. Environ.Microbiol. 70:5315-5322.
Pridmore, R.D., B. Berger, F. Desiere D. Vilanova, C. Barretto, A.-C.Pittet, M.-C. Zwahlen, M. Rouvet, E. Altermann, R. Barrangou,
B. Mollet, A. Mercenier, T.R. Klaenhammer, F. Arigoni and M.A. Schell. 2004. The genome Sequ Altermann, E. , W. M.
Russell, M.A.Azcarate-Peril, R. Barrangou, B.L.Buck, O. McAuliffe, N. Souther, A. Dobsen, T. Doung, M. Callanan, S. Lick,
A. Hamrick, R. Cano, and T.R. Klaenhammer. 2005. Complete genome sequence of the probiotic lactic acid bacterium
Lactobacillus acidophilus. NCFM. Proc. Nat. Acad. Sci. USA 102: 3906-3912.
Mohamadzadeh, M., S. Olson, W.V. Kalina, G. Ruthel, G.L. Demmin, K.L. Warfield, S.Bavari, and T. R. Klaenhammer. 2005.
Lactobacilli activate human dendritic cells that skew T cells toward T helper 1 polarization. Proc. Nat. Acad. Sci. USA.
102:2280-2285.
Bruno-Barcena, J.M. M.A. Azcarate-Peril, T.R. Klaenhammer, and H.M. Hassan. 2005. Marker-free chromosoaml integartion of the
managanese superoxide dismutase gene (sodA) from Streptococcus thermophilus into Lactobacillus gasseri. FEMS
Microbiology Letters 246: 91-101.
Lu, Z., E.Altermann, F.Breidt, P. Predki, H.P. Fleming and T.R. Klaenhammer. 2005. Sequence analysis of the Lactobacillus
plantarum bacteriophage phi JL-1. Gene 348: 45-54.
Klaenhammer, T.R., A. Azcarate Peril, R. Barrangou, T. Doung and E. Altermann. 2005. Genomic perspectives on probiotic lactic
acid bacteria. Bioscience Microflora 24:31-33.
Klaenhammer, T.R., R. Barrangou, B.L. Buck, M.A. Azcarate-Peril, E.Altermann. 2005. Genomic features of lactic acid bacteria
effecting bioprocessing and health. FEMS Microbiology Reviews.29:393-409.
McAuliffe, O., R. J. Cano, and T.R. Klaenhammer. 2005. Genetic analysis of two bile salt hydrolase activities in Lactobacillus
acidophilus NCFM. Appl. Environ. Microbiol. 71:4925-4929.
Azcarate-Peril, M.A., O. McAuliffe, E. Altermann, S.Lick, W. M. Russell, and T.R. Klaenhammer. 2005. Microarray Analysis of a
Two-Component Regulatory System Involved in Acid Resistance and Proteolytic Activity in Lactobacillus acidophilus. Appl.
Environ. Microbiol. 71: 5794-5804.
Buck, B.L., Eric Altermann, Tina Svingerud, and Todd R. Klaenhammer. 2005. Functional Analysis of Putative Adhesion Factors in
Lactobacillus acidophilus NCFM. Appl. Environ.. Microbiol. 71: 8344-8351.
PHS 398/2590 (Rev. 05/01)
Page
Continuation Format Page
Principal Investigator/Program Director (Last, First, Middle):
Altermann, E., and T.R. Klaenhammer. 2005. PathwayVoyager: pathway mapping using the Kyoto Encyclopedia of genes and
genomes (KEGG) database. BMC Genomics 6:60.
Klaenhammer, T.R., deVos, W.M., and Mercenier, A. 2005. Genomics of probiotic lactic acid
bacteria: impacts on functional foods. In Bioprocesses and biotechnology for functional foods and nutraceuticals. Marcel
Dekker, NY, pp 63–78.
Altermann, E. , W. M. Russell, M.A.Azcarate-Peril, R. Barrangou, B.L.Buck, O. McAuliffe, N., Souther, A. Dobsen, T. Doung, M.
Callanan, S. Lick, A. Hamrick, R. Cano, and T.R. Klaenhammer. 2005. Complete genome sequence of the probiotic lactic acid
bacterium Lactobacillus acidophilus NCFM. Proc. Nat. Acad. Sci. USA 102: 3906-3912.
Barrangou, R., M.A. Azcarate-Peril, T.Duong, S.B. Conners, R.M. Kelly, and T.R. Klaenhammer. 2006. Global analysis of
carbohydrate utilization by Lactobacillus acidophilus using cDNA microarrays. Proc. Nat. Acad. Sci. USA 103: 3816-3821.
Sturino, J., and T.R. Klaenhammer. 2006. Engineered bacteriophage defenses systems in bioprocessing. Nature Reviews 4:395-404.
Ventura M, Canchaya C, Bernini V, Altermann E, Barrangou R, McGrath S, Claesson MJ, Li Y, Leahy S, Walker CD, Zink R,
Neviani E, Steele J, Broadbent J, Klaenhammer TR, Fitzgerald GF, O'toole PW, van Sinderen D.2006. Comparative genomics
and transcriptional analysis of prophages identified in the genomes of Lactobacillus gasseri, Lactobacillus salivarius, and
Lactobacillus casei. Appl Environ Microbiol. 72:3130-3146.
Engelbrektson, A.L., J.R. Korzenik, M.E. Sanders, B.G.Clement, G. Leyer, T.R. Klaenhammer & C.L.Kitts. 2006. Analysis of
treatment effects on the microbial ecology of the human intestine. FEMS Microbial Ecol. 57:239- 250.
Makarova K, Slesarev A, Wolf Y, Sorokin A, Mirkin B, Koonin E, Pavlov A, Pavlova N, Karamychev V, Polouchine N, Shakhova V,
Grigoriev I, Lou Y, Rohksar D, Lucas S, Huang K, Goodstein DM, Hawkins T, Plengvidhya V, Welker D, Hughes J, Goh Y,
Benson A, Baldwin K, Lee JH, Diaz-Muniz I, Dosti B, Smeianov V, Wechter W, Barabote R, Lorca G, Altermann E,
Barrangou R, Ganesan B, Xie Y, Rawsthorne H, Tamir D, Parker C, Breidt F, Broadbent J, Hutkins R, O'Sullivan D, Steele J,
Unlu G, Saier M, Klaenhammer T, Richardson P, Kozyavkin S, Weimer B, Mills D. Comparative genomics of the lactic acid
bacteria. 2006 Proc Natl Acad Sci U S A. 103:15611-15616.
Duong, T., R. Barrangou, W.M. Russell, and T.R. Klaenhammer. 2006. Characterization of the tre locus and analysis of trehalose
cryoprotection in Lactobacillus acidophilus. Appl. Environ. Microbiol. 72: 1218-1225.
Azcarte-Peril, M.A., B. Bruno-Barcena, H.M. Hassan, and T.R. Klaenhammer. 2006. Transcriptional and functional analysis of
oxalyl-coenzyme A (CoA) decarboxylase and formyl-CoA transferase genes from Lactobacillus acidophilus. Appl. Environ.
Microbiol. 72:1891-1899.
Klaenhammer, T.R., Pfeiler, E., and Duong, T. 2007. Genomics and Proteomics of Food-Borne Microorganisms. In Doyle, Beuchat,
and Montville (eds.). Food Microbiology: Fundamentals and Frontiers, 3rd edition. ASM Press, Washington, DC. pp 935-952.
Pfeiler, E.A., M. A. Azcarate-Peril, and T. R. Klaenhammer. Characterization of a two-component regulatory system implicated in the
bile tolerance of Lactobacillus acidophilus NCFM. J. Bacteriol . 189: 4624-4634.
Callanan, M.J., W.M. Russell, T. R. Klaenhammer. 2007. Modification of Lactobacillus -glucuronidase activity by random
mutagenesis. Gene 389: 122-127.
Dobson, A.E., R.B. Sanozky-Dawes, and T.R. Klaenhammer. 2007. Identification of an operon and inducing peptide involved in the
production of lactacin B by Lactobacillus acidophilus. J. Appl. Microbiol. 103: 1766-78.
Klaenhammer, T.R., Pfeiler, E., and Duong, T. 2007. Genomics and Proteomics of Food-Borne Microorganisms. In Doyle, Beuchat,
and Montville (eds.). Food Microbiology: Fundamentals and Frontiers, 3rd edition. ASM Press, Washington, DC, pp 935-951.
Klaenhammer, T.R. Probiotics and Prebiotics. 2007. In Doyle, Beuchat, and Montville (eds.). Food Microbiology: Fundamentals
and Frontiers, 3rd edition. ASM Press, Washington, DC, pp 891-907.
Durmaz, E. and T.R. Klaenhammer. 2007. Abortive phage resistance mechanism AbiZ speeds the lysis clock to cause premature
lysis of phage infected Lactococcus lacts J. Bacteriol. 189:1417-1425.
Duong, T. and T.R. Klaenhammer. 2008. Functional genomics of lactic acid bacteria. In, Therapeutic Microbiology: Probiotics and
Other Strategies. J. Versalovic and M. Wilson, Eds. Pp 193-204. ASM Press, Washington, DC.
Durmaz. E., Miller, M.J., Azcarate-Peril, M. A., Toon, S. P.,and T. R. Klaenhammer. 2008. Genome Sequence and Characteristics of
Lrm1, a prophage from industrial Lactobacillus rhamnosus strain M1. Appl. Environ. Microbiol. 74: 4601-4609.
Azcarate-Peril MA, Altermann E, Goh YJ, Tallon R, Sanozky-Dawes RB, Pfeiler EA, O'Flaherty S, Buck BL, Dobson A, Duong T,
Miller MJ, Barrangou R, Klaenhammer TR. 2008. Analysis of the genome sequence of Lactobacillus gasseri ATCC 33323
reveals the molecular basis of an autochthonous intestinal organism. Appl Environ Microbiol. 74:4610-4625
Mohamadzadeh, M., T. Duong, T. Hoover, and T.R. Klaenhammer. 2008. Targeting mucosal dendritic cells with microbial antigens
from probiotic lactic acid bacteria. Expert Reviews Vaccines 7:163-174.
Mohamadzadeh, M., T. Duong, S. J. Sandwick, T. Hoover, and T. R. Klaenhammer. 2009. Dendritic cell targeting of Bacillus
anthracis protective antigen expressed by Lactobacillus acidophilus protects mice from lethal challenge. Proc. Nat. Acad. Sci.
USA. www.pnas.org/cgi/doi/10.1073/pnas.0900029106
Number of Students Trained: 40, + 3 in progress, with 6 Ph.D. students awarded the Kenneth R. Keller Research
Award for the most outstanding Ph.D.Dissertation in the College of Agriculture and Life Sciences, NCSU.
Number of Post-Doctoral Scholars Trained: 21
PHS 398/2590 (Rev. 05/01)
Page
Continuation Format Page