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Potential = stored Kinetic = motion ATP (adenosine triphosphate) The high energy is stored in the last P bond Adenine Base 3 Phosphates Ribose Sugar Our bodies have less than a gram of ATP! Our cells must recycle ATP We use the energy in glucose to reattach phosphates to ADP. This process is called Cellular Respiration • Occurs in the cytoplasm and mitochondria 1 Glucose = 38 ATP Four stages: 1. Glycolysis 2. Transition Reaction 3. Citric Acid Cycle 4. Electron Transport Chain NADH High-energy electrons carried by NADH NADH FADH2 and GLYCOLYSIS CITRIC ACID CYCLE Pyruvate Glucose Electron Transport Chain Mitochondrion Cytoplasm ATP Substrate-level phosphorylation CO2 ATP CO2 Substrate-level phosphorylation ATP Oxidative phosphorylation Glucose (6-carbon chain) is broken down to two Pyruvates (3-carbon chains) This cost 2 ATPs, but produced 4 ATPs Net gain = 2 ATPs In the process, 2 H+ ions combine with NAD+ to form NADH molecules – electron carriers Net gain = 2 NADH 2 Pyruvates (3-carbon chains) react with coenzyme A (CoA) to form 2 acetyl CoA’s (2carbon chains) CoA is like a trailer loading up carbon to get into the Mitochondria Output = 2 CO2 Net gain = 2 NADH NAD+ NADH + H+ CoA Pyruvate Acetyl CoA (acetyl coenzyme A) CO2 Figure 6.8 Coenzyme A In Mitochondria CoA drops off 2-C chain which combines with a 4-C compound to form a 6-C chain Through the cycle Carbons are snapped off and released as CO2 For 2 trips around the cycle: ◦ ◦ ◦ ◦ 4 6 2 2 CO2 released NADH made FADH2 made ATP made Located on inner membrane of Mitochondria Electrons from NADH and FADH2 ◦ Travel down the electron transport chain to oxygen, which picks up H+ to form water (H2O) Electron movement causes an H+ gradient H ions rush through ATP enzyme producing: ◦ NADH 3 ATP ◦ FADH2 2 ATP 10 NADH = 30 ATP 2 FADH2 = 4 ATP H+ Intermembrane space Inner mitochondria l membrane Mitochondrial matrix . Protein complex H+ FADH2 Electron flow NADH H+ H+ H H+ Electron carrier H+ H+ ATP synthase FAD NAD+ H 1O + 2H+ 2 2 + H+ H+ H2O Electron Transport Chain OXIDATIVE PHOSPHORYLATION Figure 6.10 H+ + ADP + P H+ ATP Chemiosmosis NADH High-energy electrons carried by NADH NADH FADH2 and GLYCOLYSIS CITRIC ACID CYCLE Pyruvate Glucose Electron Transport Chain Mitochondrion Cytoplasm ATP Substrate-level phosphorylation CO2 ATP CO2 Substrate-level phosphorylation ATP Oxidative phosphorylation Light energy 6 CO2 + 6 Carbon dioxide H2O Water C6H12O6 + 6 Glucose O2 Oxygen gas Through the process of photosynthesis, plants can store energy by making glucose. Plants are autotrophs ◦ They make their own food (sugars) They are so good at it that they make enough for us too! Plant cells full of chloroplasts! Thylakoids – stacks of chlorophyll inside the chloroplast Wavelengths of visible light are absorbed by plant pigments This drives the light reactions of photosynthesis Increasing energy 10–5 nm 10–3 nm Gamma rays X-rays 1 nm 103 nm UV 1m 106 nm Microwaves Infrared 103 m Radio waves Light Reflected light Visible light 380 400 500 600 700 750 Wavelength (nm) 650 nm Chloroplast Absorbed light Transmitted light Photo system 1 & 2 ◦ Photons from sun split water into O2 (released) and H+ ◦ Photons absorbed by chlorophyll excite H+ ions ◦ H+ ions are passed between photosystems until they reach final electron carriers = NADPH ◦ The final H+ ion cycles six times to make a total of 18 ATPs ◦ Net gain = 12 NADPH and 18 ATP ◦ 18 ATPs will help make 1 glucose ATP and NADPH from light reaction powers sugar production in the Calvin cycle Plant is taking in CO2 3 (5-Carbon) chains cycle around picking up CO2 Each cycle makes half a sugar, so two cycles will make a full sugar Light energy 6 CO2 + 6 Carbon dioxide H2O Water C6H12O6 + 6 Glucose O2 Oxygen gas