Download FUTURE WORK

BSL3
To Infect
Inhalation
Laboratory
System
Animals
Team Members
Nick Anders – Team Leader
Stacey Hoebel – Communications
Jeff Swift – BWIG
Maja Middleton – BSAC
Kayla Ericson – BWIG
Austin Ramme - BWIG
Advisor
Professor Willis Tompkins
Client
Adel Talaat
Assistant Professor
Department of Animal and Biomedical Sciences
Abstract

Our objective is to build a safe system for
infecting small animals with a Biological Safety
Level 3 pathogen. The design must incorporate
safety features to prevent the escape of the
infectious materials from the infection chamber
to the surrounding environment. The research
will be used to study pathogenesis of
tuberculosis. Ultimate outcome of this study is
the development of novel drugs and vaccines
that can cure tuberculosis patients.
Problem Statement
Develop
a safe inhalation system for animal
infection
Should be able to infect animals ranging in
size from mice, rats, and guinea pigs to
rabbits
Should be equipped with pathogen escape
prevention measures to insure safety in the
surrounding environment
Background

Client’s goals of animal infection study
– To study the pathenogenesis of tuberculosis
(BSL3)
– To develop a cure for TB patients

Safety Precautions
– Proper training and protective clothing
– Non-porous material
– Autoclave in work zone
– HEPA filtration
– Sealed exhaust system with directional airflow
Current Device in Use
Main chamber: 75 cm x 50 cm x 15 cm
 Inner chambers: radius 8 cm, height 12 cm
 Nebulizer, Ethanol Trap, HEPA Filter
 Tupperware

Current Models available
•Glas-Col Manufactures a model for
$15,000
•Independent system
•UV light and HEPA filters incorporated
•Automated controls
•UW-Madison Engineering Shops make a
model for $33,000
•Independent system
•Stainless Steel Cylinder
•Automated Controls
•Clamps for seal
Design Specifications

Dimensions:
– Length: 25 cm – 75 cm
– Width: 40 cm – 30 cm
– Height: 10 cm – 70 cm
Withstand the Autoclaving Process
 Storage at Room Temperature
 Air Tight Structure
 Deliver 5 mL of culture in 20 minutes
 Service Life: 50 to 100 Uses

Alternative Design 1:

Pros
– Dual Chamber System
– Split Air Flow
– Latex Flange around
Neck

Air input
Cons
– Specific only to mice
– Too difficult to
manufacture
– Too expensive
animal
Air output
Animal head
Air input
Alternative Design 2:

Pros
– Autoclavable
– Even pathogen delivery
 Nose piece
 Split Tubing

Cons
– Too difficult to manufacture
 Nose piece
 Tubing
– Too expensive
– Infects only 4 animals at
once
Alternative Design 3:
•Pros
•Jars for various
animal sizes
•Latex diaphragms
for restraint
•Circulation fan for
distribution
•Fits under fume
hood
•Cons
•Too difficult to
manufacture
•Too expensive
•Lid with Gasket
Tape and Output
Valve
•PVC Pipe Stand
•Stainless Steel
Mesh Shelf
•Input Valve with
Elbow for Air Flow
•Latches
System Advantages

Fully Autoclavable

Air Tight System
– Increased Efficiency
– Increased Safety

Even Distribution of Pathogen

Increased Holding Container Size
– Small Animals to Large Animals
– Greater Numbers per Trial
Manufacturing: Clamp Attachment
Nut
Clamp Assembly

Holes Drilled at Equal
Intervals

Holes Filed and
Finished to Remove
Sharp Edges

Teflon Gasket Custom
Fit to Screws

Screws, Teflon Washer,
Nut, and Clamp
Attached
Screw
Teflon Washer
Attached Clamp
Manufacturing: Lid

Lid
– 3 layers of PTFE gasket tape on inside lip
– Creates seal between pot and lid
Manufacturing: Inflow
Inflow Elbow
Inflow Hose Nipple

½” Diameter Hole
Drilled with Step Drill

Elbow Positioned on
Threaded End of Hose
Nipple

Hose Nipple Welded
to Pot

Elbow positioned to
45o to promote air
circulation
Manufacturing: Outflow
Outflow Hose Nipple

½” Diameter Hole
Drilled with Step Drill

Hose Nipple Welded
to Pot
Cost







48 qt. Stainless Steel Stockpot
Draw Latches (8)
Woven Wire Cloth
Stainless Hose Adapters (2)
45o Elbow Fitting
PTFE Gasket Tape
Welding and Labor
TOTAL
$90.00
$33.40
$33.00
$16.50
$11.60
$32.50
$50.00
$267.00
Design Matrix
Design1
4
Design2
3
Design3
2
Final
9
Ease of
Manufacture
5
6
3
7
Cleaning
5
4
5
8
Safety
7
6
7
7
Overall
21
19
17
Cost
31
FUTURE WORK
TESTING
–Test with water and non-pathenogenic bacteria
Efficiency
Consistency
Safety
Air Flow
Improved
animal holding chambers
–Nose/Head only
References

www.McMaster-Carr.com

www.EastmanOutdoors.com

www.glascol.com/products/biotimag/biotech.html

www.hc-sc.gc.ca/pphb-dgspsp/publicat/lbg-ldmbl-96/

www.nalgene.com
Special Thanks
Professor Tompkins
(Advisor)
 Orrie Lokken (ME Shop
Supervisor)
 Adel Talaat (Client)


Nick Anders (our fallen
leader)