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BIO 274-01 Exam 4 Name ___________________ Multiple Choice (3 pts each) 1. a. b. c. d. All of the following are a function of roots except absorption of water and nutrients converting light energy to chemical energy conduction of absorbed minerals anchoring plant in the soil 2. a. b. c. d. All of the following are true about fibrous root systems except have several main roots are denser than tap roots develop from the radicle only all of the above are true 3. a. b. c. d. Adventitious roots are defined as roots that have several main roots that form a dense mass of roots roots that have one main root from which lateral roots branch roots that do not arise from another root or the embryo none of the above are true 4. a. b. c. d. Climbing vines have a tap root system a fibrous root system both a and b an adventitious root system 5. a. b. c. d. The root cap functions to protect the root provide nutrients to the root is located at the tip of the root both a and c 6. a. b. c. d. The epidermis anchors the plant in the soil converts sunlight to chemical energy is the reproductive structure of the plant none of the above 7. All are true about the quiescent center except a. it is located in the center of the RAM b. it is the zone where root initials are maintained c. it is characterized by low mitotic activity d. it originates from the embryo proper 8. a. b. c. d. Primary roots are formed in the embryo only from the lower tier of the embryo from the hyphophysis both b and c 9. a. b. c. d. The hyphophesis is the uppermost cell of the suspensor undergo’s divisions that result in the QC produces lateral roots both a and b 10. Growth of lateral roots is triggered by a. ethylene b. gibberellic acid c. auxin d. none of the above ---------------------------------------------------------------------------------------------------------Short Answer 11. The RAM differentiates into three primary meristems. What are they and what develops from each one? (6 pts) Protoderm - epidermis Ground meristem - cortex Procambium – xylem, phloem 12. The root has three zones of development. List and describe them. (6 pts) Root apical meristem – zone of cell division Region of elongation – no cell division, rapid growth by elongation Region of maturation – zone of differentiation, cells complete differentiation into specialized cells 13. What is the “quiescent center? The QC is analogous to what zone in the SAM? (6 pts) The QC is located in the center of the RAM, it is the zone where root initials are maintained. It is analogous to the central zone of the SAM. 14. What determines the identity of a cell (specialized function) in the root? (5 pts) The location of the cell in the root. 15. What will happen if you use a laser to destroy cells in the cortical layer? (6 pts) The cells of the adjacent layers will invade the empty space and assume the cell division patter of cortical initials. 16. Where is growth of lateral roots initiated? Why do these roots only develop in the late cell elongation, early cell differentiation zones? (5 pts) In the pericycle, this is the zone where cells are differentiated enough to have a specific function. 17. How do plant hairs differ from lateral roots? (5 pts) Plant hairs are found on root epidermis, lateral roots originate from the pericycle. 18. Complete the following table. (6 pts) Gene Mutation Phenotype of Plants with Mutation Fass (fs) Enlarged roots resulting from enlarged vascular cylinder and multiple cortical layers Superroot (sur1) Several lateral and adventitious roots; produces greater levels of auxins HOBBIT (HBT) Interference with cell division and cell type specification; root cap cells and Proposed Function of Gene a. b. Gene required for the maintenance of cell division and acquisition of the QC cells cannot be maintained differentiated state a. regulates cell growth b. regulates auxin production 19. Mutations in the HOBBIT (HBT) gene interfere with what two functions in Arabidopsis roots? (5 pts) Cell division and cell type specification. 20. The HBT gene encodes a homolog of the CDC27 subunit of the Anaphase Promoting Complex. What is the specific function of this complex? (5 pts) Cell cycle progression, it degrades proteins to allow M phase progression. 21. SCR and SHR encode what type of regulatory proteins. What is the function of these genes? (6 pts) GRAS-type transcription factors required for asymmetric division of ground tissue stem cells and endodermis specification. 22. What phenotype is observed in plant that have a mutation in the rml1 and rml2 genes? (4 pts) Very short primary roots, lateral roots that do not elongate.