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EXPT. NAME: NO. Estimation of, Polyphenols, by by Colorimetric Method Aim To estimate present method. în the amount qiven Sample Equipments Test tubes pipettes Page No.: YOUv DA1/0LJa2 ot by by polyphenol Colorimetri colorimeter and Cuvettes. Reaqents : 0-1 N Folin's Ciocaltaeucagent 1 in 1a dtlution l o % 5odium Palyphena carbonate standard9uaiacol 0.12 20 mqper ml ot quaiacol standard in 0 ethanol. :5mlot stock standard sWorkinq StandardL: is diluted to loo mlConcentration Imglml Principle 3 The hydroxsl qraups are 0xidized by he phosphor tungsic acid reducin4 agent Eolin's Ciocataeu reagent which Can be me asured to product. blue Colarproduct, at 7o0 nm. Teacher's Signature EXPT. Page No. NAME: NO. YouvA Date Proceduxe Pipete out standard with O.2 to 1 ml ot workinq which Corre sp onds 1 m mq respechvely. and 0.5 ml 0.6, 0.8 to To all 0.2, 0.4, test reaqenot ofFolin Cioaltaeu The volume of alltest ubes i s made upto 3.5 ml using disilled water Then added 5m of LO Sodium carbanate Then tubes is added the sest tube are for a n hour Added water and measured All allowed to 6m o Hhe the Stand distilled absorbance at 100 nm- Preparation o lqot exracted 3 ml with of H unil the Then 6.S dest Sample : Hower5ample is_colle.cfe.d experlment- 1 m of 70Jethano acetonitrile extract Sample and or and and methanol Lat s 7o is Color leSS. aken tor the teated a s above was Teacher's Signature: Qbservahian Concentrathon Sr.No Par Hculars 1. Standard B solution (mlo.0 2. Concentration (ug) 3 4 Test sample Disilled S 0.2 wakr (ml)3.S33 6 0 (ml) Sodium Add 6ml S3 Ss S4 0-4 . 6 T 1 400 600 &00 31 2-9 2-7 Carbonate the of of 9. Optical density minture disilled 2.S 3 5 Cmd) Incubate stand 1mq /ml wor Finq (ml) Folin ciocalteau reagent g. S 200 5 7. of for qn our water to O.9 0-14 all at room fest 0.23 0.3o tem p. tubes. 0 35 0. 13 EXPT. NAME: NO. Page NO. YoUVA Date Calaulaions From he 0.18 9raph est Sqmple OD of L0 u Tes+ of 0fpolyphenol Contains here tore 490x o0 O S corres pon ds to ie, 0:5 ml of 1gof polyp heno o0 m ot Hower exiract contain8 2000ug 98 ma. ReSult The 106 m amountot poly pheno _0f 92000U qiven sample present in is 9R m -x- Teacher's Signature-