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18.4 Bacteria and Archaea KEY CONCEPT Bacteria and archaea are both single-celled prokaryotes. 18.4 Bacteria and Archaea Prokaryotes are widespread on Earth. • Prokaryotes can be grouped by their need for oxygen. – obligate anaerobes are poisoned by oxygen – obligate aerobes need oxygen – facultative aerobes can live with or without oxygen Evaluate: Bacteria are often associated with illness. Why is this a misconception? 18.4 Bacteria and Archaea Bacteria and archaea are structurally similar but have different molecular characteristics. • Bacteria commonly come in three forms. – rod-shaped, called bacilli – spiral, called spirilla or spirochetes – spherical, called cocci Spirochaeta: spiral Lactobacilli: rod-shaped Enterococci: spherical • Archaea have many shapes. Spirochaeta: spiral 18.4 Bacteria and Archaea • Bacteria and archaea have similar structures. – plasmid – flagellum pili plasma – pili membrance chromosome cell wall plasmid This diagram shows the typical structure of a prokaryote. Archaea and bacteria look very similar, although they have important molecular differences. flagellum 18.4 Bacteria and Archaea • Bacteria and archaea have molecular differences. – The amount of peptidoglycan within the cell wall can differ between bacteria GRAM NEGATIVE GRAM POSITIVE – Archaea have different lipids entirely 18.4 Bacteria and Archaea • Gram staining identifies bacteria. – stains polymer peptidoglycan – gram-positive stains purple, more peptidoglycan – gram-negative stains pink, less peptidoglycan Gram-negative bacteria have a thin layer of peptidoglycan and stain red. Gram-positive bacteria have a thicker peptidoglycan layer and stain purple. 18.4 Bacteria and Archaea Bacteria have various strategies for survival. • Prokaryotes exchange genes during conjugation. In conjugation genetic material transfers between prokaryotes, producing genetic variation. conjugation bridge • Bacteria may survive by forming endospores. Endospores are a specialized cell with a TEM; magnification 6000x thick, protective cell wall. Endospores can last for centuries. Connect: Why are disinfectants alone not enough to kill all types of bacteria? 18.4 Bacteria and Archaea Prokaryotes provide nutrients to humans and other animals. • Prokaryotes live in digestive systems of animals. – make vitamins – break down food – fill niches Figure: These bacteria, found in human intestines, are beneficial to our health. They produce B vitamins and keep out harmful microbes. (colored SEM; magnification 6300X) 18.4 Bacteria and Archaea • Bacteria help ferment many foods. – yogurt, cheese – pickles, sauerkraut – soy sauce, vinegar 18.4 Bacteria and Archaea Prokaryotes play important roles in ecosystems. • Prokaryotes have many functions in ecosystems. – photosynthesize – recycle carbon, nitrogen, hydrogen, sulfur – fix nitrogen Figure: Root nodules of this white clover contain nitrogen-fixing bacteria. Symbiotic bacteria convert nitrogen from the atmosphere (N2) into a form usable by the clover. In return, the plant produces carbohydrates that the bacteria can consume. This SEM (90X) shows root nodules, containing nitrogen-fixing bacteria, of a white clover. 18.4 Bacteria and Archaea • Bioremediation uses prokaryotes to break down pollutants. – oil spills – biodegradable materials Figure: Oil spill clean-up, men, cleaning. Sometimes workers will spray bacteria over the contaminated are so the bacteria can digest the chemicals, converting them into harmless or less-harmless compounds. 18.4 Bacteria and Archaea Some bacteria cause disease. • Bacteria cause disease by invading tissues or making toxins. • A toxin is a poison released by an organism. Figure: Clostridium botulinum causes a serious illness called botulism. Food contamination by this bacterium often comes from improper home canning. 18.4 Bacteria and Archaea • Normally harmless bacteria can become destructive. – may colonize new tissues Figure: Streptococcus bacteria are commonly found on skin. They are fairly harmless unless they come in contact with tissues they do not normally colonize, such as muscle or fat. This can occur through open wounds. 18.4 Bacteria and Archaea • Normally harmless bacteria can become destructive. – immune system may be lowered Apply: Potato salad left out at a picnic is sometimes a source of food poisoning. Which bacterium mentioned above is the most likely culprit? Explain. 18.4 Bacteria and Archaea Antibiotics are used to fight bacterial disease. • Antibiotics may stop bacterial cell wall formation. • Antibiotics do not work on viruses. • Prevention is best method to fight bacterial disease. 18.4 Bacteria and Archaea Bacteria can evolve resistance to antibiotics. • Bacteria are gaining resistance to antibiotics. – overuse A bacterium carries – underuse genes for antibiotic resistance on a plasmid. – misuse • Antibiotics must be A copy of the plasmid is transferred through used properly. conjugation. Infer: Why don’t antibiotics affect our bodies’ cells? Connect: How can you use “superbugs” as an example of natural selection? Resistance is quickly spread through many bacteria. 18.4 Bacteria and Archaea Archaea are extremophiles • Archaea exist or “love” extreme conditions – Extremely hot (thermophile – High salt (halophile) – High acidity (acidophile) pH: Some bacteria can live at a pH of 0 High temp: The highest temperature archaea have been known to live at is 230 degrees Fahrenheit (110 Celsius)