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G-Biosciences/Genotech: St. Louis, MO, USA; www.GBiosciences.com, email: [email protected] Product: TBS, Tris buffered saline [20X] Dry Buffer Pack Catalog #: RC-148 Components Catalog # Quantity TBS RC-148 for 20L [LU 1.12.10/JK-IA] Material Safety Data Sheet Section 1: Chemical Identification Name: TBS Catalog #: a component of product Cat # RC-148 indicated above Section 2: Composition/Information on Ingredients TBS contains the following: Chemical name: CAS#: Tris 77-86-1 Potassium chloride 7447-40-7 Sodium chloride 7647-14-5 Tris hcl 1185-53-1 Section 3: Hazards Identification Label precautionary statements. Irritant. Irritating to eyes, respiratory system and skin. Wear suitable protective clothing. Section 4: First-Aid Measures After eye contact: Immediately flush eyes with copious amounts of water for at least 15 minutes. Call a physician. After skin contact: Immediately wash skin with soap and copious amounts of water. After Inhalation: Remove to fresh air. If not breathing give artificial respiration. If breathing is difficult, call a physician. After swallowing: Wash out mouth with water provided person is conscious. Call a physician. Section 5: Fire Fighting Measures Extinguishing Agents: Water spray, carbon dioxide, dry chemical powder or appropriate foam. Special fire fighting procedures: Wear self-contained breathing apparatus and protective clothing to prevent contact with skin and eyes. Unusual fire and explosions hazards: Emits toxic fumes under fire conditions. Section 6: Accidental Release Measures Human Safety Precautions: Exercise appropriate precautions to minimize direct contact with skin or eyes and prevent inhalation of dust. Cleaning/Collecting Measures: Sweep up, place in a bag and hold for waste disposal. Avoid raising dust. Ventilate area and wash spill site after material pickup is complete. Section 7: Handling and Storage Refer to section 8. Section 8: Exposure Controls/Personal Protection Personal protective equipment: Chemical safety goggles, compatible chemical-respirator, NIOSH/MSHA-approved respirator, and safety shower and eye bath. General Protective and Hygiene Measures: Mechanical exhaust required. Do not breathe dust. Avoid inhalation. Avoid contact with eyes, skin and clothing. Avoid prolonged or repeated exposure. Wash thoroughly after handling. Keep tightly closed. Store in cool dry place. Section 9: Physical and Chemical Properties Form: Solid Color: none. Odor: None Section 10: Stability and Reactivity Thermal Decomposition/Conditions to be avoided: Avoid strong oxidizing agents. Dangerous Reactions: None known. Dangerous Products of Decomposition: Carbon monoxide, carbon dioxide, nitrogen oxides. Section 11: Toxicological Information On the skin: May be harmful if absorbed through the skin. May cause skin irritation. On the eye: May cause eye irritation. Inhalation: May be harmful if inhaled. Material may be irritating to mucous membranes and upper respiratory tract. Ingestion: May be harmful if swallowed. Section 12: Ecological Information: Data not yet available Section 13: Disposal Considerations Recommendation: Contact a licensed professional waste disposal service to dispose of this material. Observe all federal, state and local environmental regulations. Section 14: Transport Information: Not dangerous goods. Safe for all transport. Section 15: Regulatory information Irritating to eyes, respiratory system and skin. In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing. Section 16: Other Information The above information is believed to be correct but does not purport to be all-inclusive and shall be used only as a guide. Geno Technology shall not be held liable for any damage resulting from handling or from contact with the above product. See reverse side of invoice or packing slip for additional terms and conditions of sale. G-Biosciences/Genotech St. Louis, MO, USA Web: www.GBiosciences.com, email: [email protected] SIGMA-ALDRICH sigma-aldrich.com Material Safety Data Sheet Version 5.0 Revision Date 03/13/2011 Print Date 11/27/2012 1. PRODUCT AND COMPANY IDENTIFICATION Product name : 1-O-Octyl-β-D-glucopyranoside Product Number Brand : : O3757 Sigma Supplier : Telephone Fax Emergency Phone # (For both supplier and manufacturer) Preparation Information : : : Sigma-Aldrich 3050 Spruce Street SAINT LOUIS MO 63103 USA +1 800-325-5832 +1 800-325-5052 (314) 776-6555 : Sigma-Aldrich Corporation Product Safety - Americas Region 1-800-521-8956 2. HAZARDS IDENTIFICATION Emergency Overview OSHA Hazards No known OSHA hazards Not a dangerous substance or mixture according to the Globaly Harmonised System (GHS). HMIS Classification Health hazard: Flammability: Physical hazards: 0 0 0 NFPA Rating Health hazard: Fire: Reactivity Hazard: 0 0 0 Potential Health Effects Inhalation Skin Eyes Ingestion May be harmful if inhaled. May cause respiratory tract irritation. May be harmful if absorbed through skin. May cause skin irritation. May cause eye irritation. May be harmful if swallowed. 3. COMPOSITION/INFORMATION ON INGREDIENTS Synonyms : n-Octyl glucoside OGP Formula Molecular Weight : : C14H28O6 292.38 g/mol CAS-No. EC-No. 1-O-Octyl-β-D-glucopyranoside 29836-26-8 249-887-8 Sigma - O3757 Index-No. Concentration - Page 1 of 6 4. FIRST AID MEASURES If inhaled If breathed in, move person into fresh air. If not breathing, give artificial respiration. In case of skin contact Wash off with soap and plenty of water. In case of eye contact Flush eyes with water as a precaution. If swallowed Never give anything by mouth to an unconscious person. Rinse mouth with water. 5. FIRE-FIGHTING MEASURES Conditions of flammability Not flammable or combustible. Suitable extinguishing media Use water spray, alcohol-resistant foam, dry chemical or carbon dioxide. Special protective equipment for fire-fighters Wear self contained breathing apparatus for fire fighting if necessary. Hazardous combustion products Hazardous decomposition products formed under fire conditions. - Carbon oxides 6. ACCIDENTAL RELEASE MEASURES Personal precautions Avoid dust formation. Avoid breathing vapors, mist or gas. Environmental precautions Do not let product enter drains. Methods and materials for containment and cleaning up Sweep up and shovel. Keep in suitable, closed containers for disposal. 7. HANDLING AND STORAGE Precautions for safe handling Provide appropriate exhaust ventilation at places where dust is formed. Conditions for safe storage Keep container tightly closed in a dry and well-ventilated place. Recommended storage temperature: -20 °C 8. EXPOSURE CONTROLS/PERSONAL PROTECTION Contains no substances with occupational exposure limit values. Personal protective equipment Respiratory protection Respiratory protection is not required. Where protection from nuisance levels of dusts are desired, use type N95 (US) or type P1 (EN 143) dust masks. Use respirators and components tested and approved under appropriate government standards such as NIOSH (US) or CEN (EU). Hand protection Handle with gloves. Gloves must be inspected prior to use. Use proper glove removal technique (without touching glove's outer surface) to avoid skin contact with this product. Dispose of contaminated gloves after use in accordance with applicable laws and good laboratory practices. Wash and dry hands. Sigma - O3757 Page 2 of 6 Eye protection Use equipment for eye protection tested and approved under appropriate government standards such as NIOSH (US) or EN 166(EU). Skin and body protection Choose body protection in relation to its type, to the concentration and amount of dangerous substances, and to the specific work-place., The type of protective equipment must be selected according to the concentration and amount of the dangerous substance at the specific workplace. Hygiene measures General industrial hygiene practice. 9. PHYSICAL AND CHEMICAL PROPERTIES Appearance Form powder Colour white Safety data pH no data available Melting point/freezing point no data available Boiling point no data available Flash point no data available Ignition temperature no data available Autoignition temperature no data available Lower explosion limit no data available Upper explosion limit no data available Vapour pressure no data available Density no data available Water solubility no data available Partition coefficient: n-octanol/water no data available Relative vapour density no data available Odour no data available Odour Threshold no data available Evaporation rate no data available 10. STABILITY AND REACTIVITY Chemical stability Stable under recommended storage conditions. Possibility of hazardous reactions no data available Conditions to avoid no data available Materials to avoid Strong oxidizing agents Sigma - O3757 Page 3 of 6 Hazardous decomposition products Other decomposition products - no data available Hazardous decomposition products formed under fire conditions. - Carbon oxides 11. TOXICOLOGICAL INFORMATION Acute toxicity Oral LD50 no data available Inhalation LC50 no data available Dermal LD50 no data available Other information on acute toxicity no data available Skin corrosion/irritation no data available Serious eye damage/eye irritation no data available Respiratory or skin sensitization no data available Germ cell mutagenicity no data available Carcinogenicity IARC: No component of this product present at levels greater than or equal to 0.1% is identified as probable, possible or confirmed human carcinogen by IARC. ACGIH: No component of this product present at levels greater than or equal to 0.1% is identified as a carcinogen or potential carcinogen by ACGIH. NTP: No component of this product present at levels greater than or equal to 0.1% is identified as a known or anticipated carcinogen by NTP. OSHA: No component of this product present at levels greater than or equal to 0.1% is identified as a carcinogen or potential carcinogen by OSHA. Reproductive toxicity no data available Teratogenicity no data available Specific target organ toxicity - single exposure (Globally Harmonized System) no data available Specific target organ toxicity - repeated exposure (Globally Harmonized System) no data available Aspiration hazard no data available Potential health effects Inhalation Ingestion Skin Sigma - O3757 May be harmful if inhaled. May cause respiratory tract irritation. May be harmful if swallowed. May be harmful if absorbed through skin. May cause skin irritation. Page 4 of 6 Eyes May cause eye irritation. Signs and Symptoms of Exposure To the best of our knowledge, the chemical, physical, and toxicological properties have not been thoroughly investigated. Synergistic effects no data available Additional Information RTECS: Not available 12. ECOLOGICAL INFORMATION Toxicity no data available Persistence and degradability no data available Bioaccumulative potential no data available Mobility in soil no data available PBT and vPvB assessment no data available Other adverse effects no data available 13. DISPOSAL CONSIDERATIONS Product Offer surplus and non-recyclable solutions to a licensed disposal company. Contaminated packaging Dispose of as unused product. 14. TRANSPORT INFORMATION DOT (US) Not dangerous goods IMDG Not dangerous goods IATA Not dangerous goods 15. REGULATORY INFORMATION OSHA Hazards No known OSHA hazards SARA 302 Components SARA 302: No chemicals in this material are subject to the reporting requirements of SARA Title III, Section 302. SARA 313 Components SARA 313: This material does not contain any chemical components with known CAS numbers that exceed the threshold (De Minimis) reporting levels established by SARA Title III, Section 313. SARA 311/312 Hazards No SARA Hazards Massachusetts Right To Know Components No components are subject to the Massachusetts Right to Know Act. Sigma - O3757 Page 5 of 6 Pennsylvania Right To Know Components 1-O-Octyl-β-D-glucopyranoside CAS-No. 29836-26-8 Revision Date CAS-No. 29836-26-8 Revision Date New Jersey Right To Know Components 1-O-Octyl-β-D-glucopyranoside California Prop. 65 Components This product does not contain any chemicals known to State of California to cause cancer, birth defects, or any other reproductive harm. 16. OTHER INFORMATION Further information Copyright 2011 Sigma-Aldrich Co. License granted to make unlimited paper copies for internal use only. The above information is believed to be correct but does not purport to be all inclusive and shall be used only as a guide. The information in this document is based on the present state of our knowledge and is applicable to the product with regard to appropriate safety precautions. It does not represent any guarantee of the properties of the product. Sigma-Aldrich Co., shall not be held liable for any damage resulting from handling or from contact with the above product. See reverse side of invoice or packing slip for additional terms and conditions of sale. Sigma - O3757 Page 6 of 6 SIGMA-ALDRICH sigma-aldrich.com Material Safety Data Sheet Version 4.2 Revision Date 05/08/2012 Print Date 11/20/2012 1. PRODUCT AND COMPANY IDENTIFICATION Product name : Pluronic® F-127 Product Number Brand : : P2443 Sigma Supplier : Telephone Fax Emergency Phone # (For both supplier and manufacturer) Preparation Information : : : Sigma-Aldrich 3050 Spruce Street SAINT LOUIS MO 63103 USA +1 800-325-5832 +1 800-325-5052 (314) 776-6555 : Sigma-Aldrich Corporation Product Safety - Americas Region 1-800-521-8956 2. HAZARDS IDENTIFICATION Emergency Overview OSHA Hazards No known OSHA hazards GHS Classification Skin irritation (Category 3) GHS Label elements, including precautionary statements Pictogram none Signal word Warning Hazard statement(s) H316 Precautionary statement(s) Causes mild skin irritation. none HMIS Classification Health hazard: Flammability: Physical hazards: 1 0 0 NFPA Rating Health hazard: Fire: Reactivity Hazard: 1 0 0 Potential Health Effects Inhalation Skin Eyes Ingestion May be harmful if inhaled. May cause respiratory tract irritation. May be harmful if absorbed through skin. May cause skin irritation. May cause eye irritation. May be harmful if swallowed. 3. COMPOSITION/INFORMATION ON INGREDIENTS Sigma - P2443 Page 1 of 6 No ingredients are hazardous according to OSHA criteria. 4. FIRST AID MEASURES General advice Consult a physician. Show this safety data sheet to the doctor in attendance. If inhaled If breathed in, move person into fresh air. If not breathing, give artificial respiration. Consult a physician. In case of skin contact Wash off with soap and plenty of water. Consult a physician. In case of eye contact Flush eyes with water as a precaution. If swallowed Never give anything by mouth to an unconscious person. Rinse mouth with water. Consult a physician. 5. FIREFIGHTING MEASURES Conditions of flammability Not flammable or combustible. Suitable extinguishing media Use water spray, alcohol-resistant foam, dry chemical or carbon dioxide. Special protective equipment for firefighters Wear self contained breathing apparatus for fire fighting if necessary. Hazardous combustion products Hazardous decomposition products formed under fire conditions. - Carbon oxides 6. ACCIDENTAL RELEASE MEASURES Personal precautions Use personal protective equipment. Avoid dust formation. Avoid breathing vapors, mist or gas. Ensure adequate ventilation. Avoid breathing dust. Environmental precautions Do not let product enter drains. Methods and materials for containment and cleaning up Pick up and arrange disposal without creating dust. Sweep up and shovel. Keep in suitable, closed containers for disposal. 7. HANDLING AND STORAGE Precautions for safe handling Provide appropriate exhaust ventilation at places where dust is formed. Conditions for safe storage Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place. 8. EXPOSURE CONTROLS/PERSONAL PROTECTION Contains no substances with occupational exposure limit values. Personal protective equipment Respiratory protection Respiratory protection is not required. Where protection from nuisance levels of dusts are desired, use type N95 (US) or type P1 (EN 143) dust masks. Use respirators and components tested and approved under appropriate government standards such as NIOSH (US) or CEN (EU). Sigma - P2443 Page 2 of 6 Hand protection Handle with gloves. Gloves must be inspected prior to use. Use proper glove removal technique (without touching glove's outer surface) to avoid skin contact with this product. Dispose of contaminated gloves after use in accordance with applicable laws and good laboratory practices. Wash and dry hands. Eye protection Safety glasses with side-shields conforming to EN166 Use equipment for eye protection tested and approved under appropriate government standards such as NIOSH (US) or EN 166(EU). Skin and body protection Choose body protection in relation to its type, to the concentration and amount of dangerous substances, and to the specific work-place., The type of protective equipment must be selected according to the concentration and amount of the dangerous substance at the specific workplace. Hygiene measures Handle in accordance with good industrial hygiene and safety practice. Wash hands before breaks and at the end of workday. 9. PHYSICAL AND CHEMICAL PROPERTIES Appearance Form powder Colour colourless Safety data pH 6.0 - 7 Melting point/freezing point no data available Boiling point no data available Flash point no data available Ignition temperature no data available Autoignition temperature no data available Lower explosion limit no data available Upper explosion limit no data available Vapour pressure no data available Density no data available Water solubility no data available Partition coefficient: n-octanol/water no data available Relative vapour density no data available Odour no data available Odour Threshold no data available Evaporation rate no data available 10. STABILITY AND REACTIVITY Chemical stability Stable under recommended storage conditions. Possibility of hazardous reactions no data available Sigma - P2443 Page 3 of 6 Conditions to avoid no data available Materials to avoid Strong oxidizing agents Hazardous decomposition products Hazardous decomposition products formed under fire conditions. - Carbon oxides Other decomposition products - no data available 11. TOXICOLOGICAL INFORMATION Acute toxicity Oral LD50 LD50 Oral - rat - 9,380 mg/kg LD50 Oral - mouse - 15,000 mg/kg Inhalation LC50 no data available Dermal LD50 LD50 Dermal - rabbit - 20,000 mg/kg Other information on acute toxicity no data available Skin corrosion/irritation Skin - rabbit - Mild skin irritation - 24 h Serious eye damage/eye irritation Eyes - rabbit - Mild eye irritation - 24 h Eyes - rabbit - No eye irritation - OECD Test Guideline 405 Respiratory or skin sensitization rabbit - Did not cause sensitization on laboratory animals. Germ cell mutagenicity Tests on bacterial or mammalian cell cultures did not show mutagenic effects. Carcinogenicity IARC: No component of this product present at levels greater than or equal to 0.1% is identified as probable, possible or confirmed human carcinogen by IARC. ACGIH: No component of this product present at levels greater than or equal to 0.1% is identified as a carcinogen or potential carcinogen by ACGIH. NTP: No component of this product present at levels greater than or equal to 0.1% is identified as a known or anticipated carcinogen by NTP. OSHA: No component of this product present at levels greater than or equal to 0.1% is identified as a carcinogen or potential carcinogen by OSHA. Reproductive toxicity no data available Teratogenicity no data available Specific target organ toxicity - single exposure (Globally Harmonized System) no data available Specific target organ toxicity - repeated exposure (Globally Harmonized System) no data available Sigma - P2443 Page 4 of 6 Aspiration hazard no data available Potential health effects Inhalation Ingestion Skin Eyes May be harmful if inhaled. May cause respiratory tract irritation. May be harmful if swallowed. May be harmful if absorbed through skin. May cause skin irritation. May cause eye irritation. Signs and Symptoms of Exposure Effects due to ingestion may include:, Diarrhoea, Weakness, To the best of our knowledge, the chemical, physical, and toxicological properties have not been thoroughly investigated. Synergistic effects no data available Additional Information RTECS: MD0911050 12. ECOLOGICAL INFORMATION Toxicity Toxicity to fish static test LC50 - other fish - > 10,000 mg/l - 96 h Method: OECD Test Guideline 203 Persistence and degradability no data available Bioaccumulative potential Bioaccumulation is unlikely. Mobility in soil no data available PBT and vPvB assessment no data available Other adverse effects no data available 13. DISPOSAL CONSIDERATIONS Product Offer surplus and non-recyclable solutions to a licensed disposal company. Contaminated packaging Dispose of as unused product. 14. TRANSPORT INFORMATION DOT (US) Not dangerous goods IMDG Not dangerous goods IATA Not dangerous goods 15. REGULATORY INFORMATION OSHA Hazards No known OSHA hazards SARA 302 Components SARA 302: No chemicals in this material are subject to the reporting requirements of SARA Title III, Section 302. Sigma - P2443 Page 5 of 6 SARA 313 Components SARA 313: This material does not contain any chemical components with known CAS numbers that exceed the threshold (De Minimis) reporting levels established by SARA Title III, Section 313. SARA 311/312 Hazards No SARA Hazards Massachusetts Right To Know Components No components are subject to the Massachusetts Right to Know Act. Pennsylvania Right To Know Components Polyethylene glycol, propoxylated CAS-No. 9003-11-6 Revision Date CAS-No. 9003-11-6 Revision Date New Jersey Right To Know Components Polyethylene glycol, propoxylated California Prop. 65 Components This product does not contain any chemicals known to State of California to cause cancer, birth defects, or any other reproductive harm. 16. OTHER INFORMATION Further information Copyright 2012 Sigma-Aldrich Co. LLC. License granted to make unlimited paper copies for internal use only. The above information is believed to be correct but does not purport to be all inclusive and shall be used only as a guide. The information in this document is based on the present state of our knowledge and is applicable to the product with regard to appropriate safety precautions. It does not represent any guarantee of the properties of the product. Sigma-Aldrich Corporation and its Affiliates shall not be held liable for any damage resulting from handling or from contact with the above product. See www.sigma-aldrich.com and/or the reverse side of invoice or packing slip for additional terms and conditions of sale. Sigma - P2443 Page 6 of 6