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Download 1891-1899 March 15, 2000
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Evidence that tristetraprolin is a physiological regulator of granulocyte-macrophage colony-stimulating factor messenger RNA deadenylation and stability by Ester Carballo, Wi S. Lai, and Perry J. Blackshear Blood Volume 95(6):1891-1899 March 15, 2000 ©2000 by American Society of Hematology Morphology of BMSCs from WT and TTP-deficient mice.BMSCs were cultured for 6 weeks as described in “Materials and Methods.” After this period, the cells were trypsinized and replated at 50 000 cells/well in 4-well Lab-Tek tissue-culture chambers. Ester Carballo et al. Blood 2000;95:1891-1899 ©2000 by American Society of Hematology Time course of GM-CSF mRNA accumulation in BMSCs stimulated with LPS.(A) 60 mm dishes containing confluent BMSCs derived from wild-type (WT) or TTP-deficient (KO) mice were prepared as described in “Materials and Methods.” Cells were then stimulated with LP... Ester Carballo et al. Blood 2000;95:1891-1899 ©2000 by American Society of Hematology Time course of GM-CSF mRNA accumulation in BMSCs stimulated with TNF- .(A) This experiment was similar to the one described in the legend to Figure 2, except that the control cells were derived from an Htz mouse, and were stimulated with TNF-α (10 ng/mL) in... Ester Carballo et al. Blood 2000;95:1891-1899 ©2000 by American Society of Hematology Evidence that the smaller species of mouse GM-CSF mRNA represents deadenylated mRNA.BMSCs were stimulated with TNF-α (10 ng/mL) for 2 or 3 hours, and total cellular RNA was harvested as described in “Materials and Methods.” As indicated, the RNA samples wer... Ester Carballo et al. Blood 2000;95:1891-1899 ©2000 by American Society of Hematology GM-CSF mRNA stability in BMSCs after addition of actinomycin D.(A) Confluent BMSCs were stimulated for 2 hours with LPS (1 μg/mL). Ester Carballo et al. Blood 2000;95:1891-1899 ©2000 by American Society of Hematology TNF- mRNA stability in BMSCs after the addition of actinomycin D.(A) Confluent BMSCs were stimulated for 2 hours with LPS (1 μg/mL). Ester Carballo et al. Blood 2000;95:1891-1899 ©2000 by American Society of Hematology Lack of response to TNF- in cells from double- and triple-KO mice.BMSCs were stimulated with LPS (1 μg/mL) or TNF-α (10 ng/mL) for 3 hours. Ester Carballo et al. Blood 2000;95:1891-1899 ©2000 by American Society of Hematology Ester Carballo et al. Blood 2000;95:1891-1899 ©2000 by American Society of Hematology Ester Carballo et al. Blood 2000;95:1891-1899 ©2000 by American Society of Hematology
