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Supplemental Digital Content 1. Real-time quantitative PCR amplicons
Target
Primers and probes
Eff.
Ref.
Bacteria
TCC TAC GGG AGG CAG CAG T
1.07
[1]
0.91
[2]
1.15
[3]
0.82
[4]
0.62
[5]
0.94
[6]
FAM-CTG ATT ACC GCG GCT GCT GGC ACTAMRA
GGA CTA CCA GGG TAT CTA ATC CTG TT
Bacteroides
GAA AGC ATT AAG TAT TCC ACC TG
fragilis
FAM-TGA AAC TCA AAG GAA TTG ACG GGGTAMRA
CGG TGA TTG GTC ACT GAC A
Escherichia coli
GTG TGA TAT CTA CCC GCT TCG C
FAM-TCG GCA TCC GGT CAG TGG CAG TTAMRA
AGA ACG GTT TGT GGT TAA TCA GGA
Bifidobacterium
TGG AAG ACG TCG TTG GCT TT
longum
FAM-CGC ACC CAC CGC A-MGB
ATC GCG CCA GGC AAA A
Lactobacillus
CAT AAA TCC AAG AAC CGC ATG G
rhamnosus GG
FAM-CTT GGC TGA AAG ATG-MGB
CAC GCC GAC AAC AGT TACT CT GC
Helicobacter pylori TTT GTT AGA GAA GAT AAT GAC GGT ATC
TAA C
FAM-CGT GCC AGC AGC CGC GGT-TAMRA
CAT AGG ATT TCA CAC CTG ACT GAC TAT C
Staphylococcus
GGC GCT TGT AAA ATT TTC GT
aureus
FAM- TTG TTC ACG ATA TGC GTA CAC GTG-
0.78
[7]
0.91
[5]
0.81
[8]
Not
[9]
TAMRA
TGC GCA AAG TTT TAT TGA ACA
Bifidobacterium
AGA ACC ACG GCG GCG TC 0.91
animalis subsp.
FAM-TGC GCT CGC CGA CG-MGB
lactis Bb-12
CGC GGT CTT CTC GAG CAC T
Lactobasillus
GAA AGA GCC CAA ACC AAG TGA TT
acidophilus La-5
FAM-TAC CAC TTT GCA GTC CTA CA-MGB
CTT CCC AGA TAA TTC AAC TAT CGC TTA
Bacteroides
CGTTCCATTAGGCAGTTGGT
determi
thetaiotaomicron
FAM-CTGAGAGGAAGGTCCCCCACATTGGA-
ned
TAMRA
ACACGGTCCAAACTCCTACG
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