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) أنموذج ( أ ) الخاص برسائل الماجستير و اطاريح الدكتوراة ( اخر شهادة University of Baghdad College Name College of Science Department Biology Full Name as written in Passport Khawla Hori Zghair e-mail [email protected] Career Assistant Lecturer Master Lecturer Assistant Professor Professor PhD Thesis Title Detection of B1 Gene From Blood of Pregnant and Abortive Women Infected With Toxoplasma gondii Year 2009 Abstract Abstract Primary maternal infection with toxoplasmosis during gestation and its transmission to the fetus continue to be the cause of tragic yet preventable disease in offspring. Serological test (ELISA) and nested polymerase chain reaction were used in an attempt to diagnose toxoplasmosis in the blood of abortive and pregnant women. One hundred twenty women were included in this study with a history of single or repeated abortion that was referred by physicians to detect anti-Toxoplasma antibodies. The study also included thirty women with high risk pregnancies whom had abnormal pregnancy outcome and thirty women with normal pregnancy were used as a control. Blood samples from all groups of women were tested for specific anti-Toxoplasma IgM and IgG antibodies by an enzyme-linked immunosorbent assay (ELISA) and for detection of B1 gene of Toxoplasma DNA by nested PCR (nPCR). ) أنموذج ( أ ) الخاص برسائل الماجستير و اطاريح الدكتوراة ( اخر شهادة Fifty-two (43.33%) of abortive women, 14(46.66%) of abnormal pregnancy displayed the presence of anti-Toxoplasma antibodies in contrast with 6(20%) in normal pregnancy. These antiToxoplasma antibodies were distributed as follows: Five (4.16%) of abortive women had IgM+, 31(25.83%) had IgG+, 16(13.33%) had both IgM+ and IgG+. 3(10%) of abnormal pregnancy had IgM+, 9(30%) had IgG+ and 2(6.66%) had IgM+ and IgG+ antibodies. Notable, the sensitivity and specificity of ELISA tests were 89.65% and 59% respectively. On the other hand, B1 gene of T.gondii DNA was detected in the blood of abortive women and abnormal pregnancy, 19 (15.83%) of abortive women and 6(20%) of abnormal pregnancy were identified to be positive for nPCR analysis in comparison with normal pregnancy. While 101(84.16%) of abortive women, 24(80%) of abnormal pregnancy and control group were revealed negative results. Remarkably, two (10.52%) of abortive women and 2(33.33%) of abnormal pregnancy with IgM+ were nPCR positive. They represent recent infection. In addition, 6(31.57%) of abortive women and 2(33.33%) of abnormal pregnancy with IgG+ were nPCR positive. They represent active infections due to reactivate of latent toxoplasmosis. Also, 5(26.31%) of abortive women and 1(16.66%) of abnormal pregnancy with both IgM+ and IgG+ were nPCR positive. They were confirming recent infection. While 6(31.57%) of abortive women and 1(16.66%) of abnormal pregnancy without anti-Toxoplasma antibodies were nPCR positive, representing a state of ) أنموذج ( أ ) الخاص برسائل الماجستير و اطاريح الدكتوراة ( اخر شهادة immunodeficiency or it could correspond to a very recent infection with insufficient production of immunoglobulin not detected by serology. PCR test in blood of pregnant and abortive women has advantages in detection of recent or active toxoplasmosis.