Download أنموذج ( أ ) الخاص برسائل الماجستير و اطاريح الدكتوراة ( اخر شهادة

) ‫أنموذج ( أ ) الخاص برسائل الماجستير و اطاريح الدكتوراة ( اخر شهادة‬
University of Baghdad
College Name
College of Science
Department
Biology
Full Name as written
in Passport
Khawla Hori Zghair
e-mail
[email protected]
Career
Assistant Lecturer
Master
Lecturer
Assistant Professor
Professor
PhD
Thesis Title
Detection of B1 Gene From Blood of Pregnant and Abortive Women Infected
With Toxoplasma gondii
Year
2009
Abstract
Abstract
Primary maternal infection with toxoplasmosis during gestation
and its transmission to the fetus continue to be the cause of tragic
yet preventable disease in offspring. Serological test (ELISA) and
nested polymerase chain reaction were used in an attempt to
diagnose toxoplasmosis in the blood of abortive and pregnant
women.
One hundred twenty women were included in this study with a
history of single or repeated abortion that was referred by
physicians to detect anti-Toxoplasma antibodies. The study also
included thirty women with high risk pregnancies whom had
abnormal pregnancy outcome and thirty women with normal
pregnancy were used as a control. Blood samples from all groups
of women were tested for specific anti-Toxoplasma IgM and IgG
antibodies by an enzyme-linked immunosorbent assay (ELISA) and
for detection of B1 gene of Toxoplasma DNA by nested PCR (nPCR).
) ‫أنموذج ( أ ) الخاص برسائل الماجستير و اطاريح الدكتوراة ( اخر شهادة‬
Fifty-two (43.33%) of abortive women, 14(46.66%) of abnormal
pregnancy displayed the presence of anti-Toxoplasma antibodies in
contrast with 6(20%) in normal pregnancy. These antiToxoplasma antibodies were distributed as follows:
Five (4.16%) of abortive women had IgM+, 31(25.83%) had IgG+,
16(13.33%) had both IgM+ and IgG+. 3(10%) of abnormal
pregnancy had IgM+, 9(30%) had IgG+ and 2(6.66%) had IgM+ and
IgG+ antibodies. Notable, the sensitivity and specificity of ELISA
tests were 89.65% and 59% respectively.
On the other hand, B1 gene of T.gondii DNA was detected in the
blood of abortive women and abnormal pregnancy, 19 (15.83%) of
abortive women and 6(20%) of abnormal pregnancy were
identified to be positive for nPCR analysis in comparison with
normal pregnancy. While 101(84.16%) of abortive women,
24(80%) of abnormal pregnancy and control group were revealed
negative results.
Remarkably, two (10.52%) of abortive women and 2(33.33%) of
abnormal pregnancy with IgM+ were nPCR positive. They
represent recent infection.
In addition, 6(31.57%) of abortive women and 2(33.33%) of
abnormal pregnancy with IgG+ were nPCR positive. They represent
active infections due to reactivate of latent toxoplasmosis.
Also, 5(26.31%) of abortive women and 1(16.66%) of abnormal
pregnancy with both IgM+ and IgG+ were nPCR positive. They were
confirming recent infection. While 6(31.57%) of abortive women
and 1(16.66%) of abnormal pregnancy without anti-Toxoplasma
antibodies
were
nPCR
positive,
representing
a
state
of
) ‫أنموذج ( أ ) الخاص برسائل الماجستير و اطاريح الدكتوراة ( اخر شهادة‬
immunodeficiency or it could correspond to a very recent infection
with insufficient production of immunoglobulin not detected by
serology.
PCR test in blood of pregnant and abortive women has
advantages in detection of recent or active toxoplasmosis.