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MIDDLESEX COMMUNITY COLLEGE MEDICAL LABORATORY TECHNICIAN PROGRAM MLT 253: MICROBIOLOGY PRACTICUM COURSE SYLLABUS Number or credits: 2.0 REQUIRED TEXT Students should refer to the textbook and lecture and laboratory course materials from MLT 253. COURSE DESCRIPTION This course is intended to provide the student experience and competency in the clinical microbiology laboratory reinforcing the knowledge and skills gained in lecture and laboratory sessions. Instrumentation, bench/manual methodologies and clinical interpretation and correlation of laboratory results will be emphasized. PREREQUISITES: MLT 203 METHOD OF INSTRUCTION Clinical faculty will utilize various methods of instruction, including but not limited to a combination of: Clinical specimens Quality control materials Microbiology automated analyzers Assay of CAP survey samples previously analyzed and stock cultures Gram stain slides Positive O&P specimens Case studies METHOD OF EVALUATION Student competency will be assessed through the following mechanisms: Formative assessment will be performed daily using a clinical evaluation instrument. Laboratory skills will be evaluated using skills assessment instruments. Summative assessment will be performed weekly using a clinical evaluation instrument. STUDENT LEARNING OUTCOMES Upon successful completion of this course, the student will be able to: 1. Demonstrate proper procedures for the collection, safe handling, and analysis of biological specimens. 2. Conduct accurate microbiology testing utilizing manual/bench methodologies and automated instrumentation to properly identify and treat pathogenic microorganisms. 3. Comply with established laboratory safety regulations. 4. Communicate effectively and demonstrate ethical behavior and professionalism. COURSE OBJECTIVES COGNITIVE- With the use of course materials and various teaching methods, the student will be able to: For each patient specimen, choose correctly appropriate media, proper atmospheric conditions, and incubation times and temperatures according to laboratory protocol. Evaluate the results of these tests for the most likely identification of microorganisms. Understand the proper specimen required for cultures of the blood, respiratory tract, stool, urine, and anaerobic sites. Describe the principles of the procedures used for identification of fungal cultures. Compare the media, incubation times and temperatures, and growth conditions necessary for the growth of mycobacteria. PSYCHOMOTOR Demonstrate proper technique in preparing smears for direct microscopic examination according to laboratory protocol. Select potential clinically significant isolates for identification. Perform appropriate biochemical and molecular biology tests for the identification of microorganisms. Utilize correctly safe techniques in handling and disposal of infectious materials according to laboratory protocol. Perform antimicrobial susceptibility testing and correlate the results to patient treatment. List the specimen requirements and identification features utilized for the recovery and identification of ova and parasites in the human body. AFFECTIVE-These objectives apply to the overall performance and participation by the student during clinical practicums at the affiliate institutions. Comply with the established dress code policy of Middlesex Community College and its’ clinical affiliates. Report to the laboratory at the scheduled time. Notify the Clinical Coordinator and the MCC MLT Program Coordinator when unable to report to the clinical practicum. Demonstrate the ability to ask pertinent questions or for assistance if needed. Demonstrate the ability to work independently within student guidelines. Communicate courteously, effectively and professionally with instructors, laboratory staff, other health care personnel, patients and visitors. Demonstrate interest and enthusiasm for the clinical laboratory science profession. Accept evaluation of performance as constructive when offered by instructors and other laboratory personnel, and follow through with suggestions made. Adhere to laboratory safety regulations in each clinical area. Maintain a clean, organized work area. Utilize reagents and supplies judiciously. Replenish supplies required in the laboratory work area. Demonstrate self-confidence in the operation of equipment and in the performance of laboratory procedures. Report patient laboratory results only to authorized personnel. Maintain the confidentiality of all privileged information. Demonstrate organizational skills through ability to coordinate the quantity of work needed to be done with the time available for its completion. Practice acceptable quality assurance as established for each clinical area. Maintain composure and work quality work under stressful conditions. PROFESSIONALISM The student is expected to conduct himself/herself in a professional manner at all times. The ability to communicate in a respectful manner under all circumstances is an expectation of a professional. The student must remember that all patient information is privileged and as such strict confidentiality must be maintained. ATTENDANCE Attendance is mandatory and will be taken each day during the clinical practicum. Students will attend all practicum meetings, except in cases of reasonable extenuating circumstances. In cases of an emergency, students should contact their Clinical Coordinator and the MCC MLT Program Coordinator when unable to report to the clinical site. COURSE TOPICS Quality control and quality assurance procedures in the microbiology lab Proper specimen collection for the recovery of pathogenic microorganisms in the human body. Evaluation of primary cultures to recognize and distinguish normal flora and clinically significant pathogens. Select appropriate media and/or broth for each type of specimen submitted to the microbiology lab. Technique/protocol required in plating different specimen types. Isolation of pathogenic bacteria. Antimicrobial susceptibility testing. Mycology and parasitology testing and identification. This work is licensed under a Creative Commons 3.0 License http://creativecommons.org/licenses/by/3.0 This workforce solution is 100% funded by a grant awarded by the U.S. Department of Labor, Employment and Training Administration, TAACCCT grant agreement # TC-22505-1160-A-25.The solution was created by the grantee and does not necessarily reflect the official position of the U.S. Department of Labor. The Department of Labor makes no guarantees, warranties, or assurances of any kind, express or implied, with respect to such information, including any information on linked sites and including, but not limited to, accuracy of the information or its completeness, timeliness, usefulness, adequacy, continued availability, or ownership. Massachusetts Community Colleges are equal opportunity employers. Adaptive equipment available upon request for persons with disabilities.