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HIBBING COMMUNITY COLLEGE COURSE OUTLINE COURSE TITLE & NUMBER: Medical Microbiology 2: MLT 2424 CREDITS: 4 (2 Lec/2 Lab) PREREQUISITES: MLT 1424: Medical Microbiology 1 CATALOG DESCRIPTION: Medical Microbiology 2 is an advanced course which introduces clinically significant fungi, mycobacterium, parasites, viral agents, and less common bacterial organism by organ system of the body. This course also covers specimen collection, specimen processing, and automated microbiology systems. OUTLINE OF MAJOR CONTENT AREAS: I. Introduction to complex microorganisms (Eukaryotes) A. Structure B. Fungi C. Mycobacterium D. Intestinal parasites E. Viruses F. Blood and tissue parasites II. Specimen processing, diseases, and identification of pathogens, including bacteria, fungi, parasites, and viruses by body system. A. Skin B. Respiratory C. Alimentary D. Genitourinary E. Nervous system F. Blood G. Wound infections III. Automated methods in microbiology COURSE GOALS/OBJECTIVES/OUTCOMES: Cognitive: Students will 1. explain safe laboratory protocols in the handling of biohazardous and infectious materials, including spill clean-up and routine disinfection of the work areas to comply with established laboratory safety regulations. 2. use quality control and quality assurance principles and practices, including MLT 2424 Hibbing Community College, a technical & community college, is an equal opportunity educator & employer 1 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. documentation, to monitor procedures, equipment and technical competency. describe collection, storage, processing, and transportation procedures for a variety of specimens collected for identification of specific pathogens, including bacteria, fungus, parasites and viruses. discuss the rationale of specimen acceptability and rejection protocols. evaluate, interpret, and relate microbiology test results to common disease processes. differentiate between and describe the clinical significance of normal and abnormal microbiology test results, emphasizing normal flora. discuss the meaning of opportunistic infections and give an example. list and give examples of various staining procedures used to identify variety of microorganisms such as AFB stains, FA tests for certain organisms, wet preps, KOH preps, etc. define the culture media and growth requirements required for the isolation of Mycobacterium tuberculosis. describe the diseases of dimorphic fungus, dermatophytes, common contaminants, and yeast. define the culture media and growth requirements of dimorphic fungi, dermatophytes, and yeast. explain different procedures used for the identification of intestinal and blood parasites. operate basic microbiology laboratory equipment to include calibration, QC, maintenance, and troubleshooting. identify causative agents in clinical case studies and present results to classmates, answering their questions. demonstrate the use of reference materials in the collection, storage, processing, and transportation of various specimens for bacterial, fungal, parasitic, or viral pathogen detection. discuss the rapid group A strep antigen test including specimen collection and handling, principle of the method, interpretation of the test results and correlation with patient disease. Psychomotor: Students will 1. consistently follow safety protocol. 2. follow procedure protocols in the collection, processing, storage, and transportation of all microbiological specimens. 3. demonstrate the isolation and identification of pathogens from mixed culture and in unknown samples. 4. recognize the presence of AFB by one of the staining methods in the samples provided. 5. recognize the presence of fungus and yeast by colonial morphology and staining. 6. perform slide culture and wet mount procedures. 7. identify Candida albicans in practice specimens. 8. process fecal and blood specimens for the examination of parasites. MLT 2424 Hibbing Community College, a technical & community college, is an equal opportunity educator & employer 2 9. recognize the presence of parasites in unknown samples of feces and blood and identify with the aid of reference materials. 10. perform a rapid strep test from a throat culture. 11. successfully collect a blood culture following prescribed protocol. MNTC GOALS AND COMPETENCIES MET: N/A HCC COMPETENCIES MET: Working Productively and Cooperatively Thinking Creatively and Critically Communicating Clearly and Effectively STUDENT CONTRIBUTIONS: The student is expected to: 1. Demonstrate and follow safe laboratory techniques 2. Attend lectures and labs 3. Participate in class activities and discussions STUDENT ASSESSMENT SHALL TAKE PLACE USING INSTRUMENTS SELECTED/DEVELOPED BY THE COURSE INSTRUCTOR. SPECIAL INFORMATION: (SPECIAL FEES, DIRECTIVES ON HAZARDOUS MATERIALS, ETC.): None AASC APPROVAL: February 16, 2012 REVIEW DATE: February 2017 MLT2424:so 021612 MLT 2424 Hibbing Community College, a technical & community college, is an equal opportunity educator & employer 3