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DE–976 11 DISTANCE EDUCATION M.Phil. (Zoology) DEGREE EXAMINATION, DECEMBER 2010. (Non Semester) RESEARCH METHODOLOGY AND LABORATORY TECHNIQUES (2006 Batch) Time : Three hours Maximum : 100 marks Answer any FIVE questions. (5 20 = 100) 1. Describe in detail the applications of the followings : (a) Phase contrast Microscopy. (b) Confocal Microscopy. (c) Electron Microscopy. (d) Micrometry 2. A biopsy sample collected from infected patients. These samples should be subjected to histochemical characterization and cytological analysis. Discuss the methods involved in it. 3. Write an essay on UV and Visible spectroscopy. Add a note on Beer-Lambert law. 4. How will you separate protein based on molecular weight and charge? 5. Give an account of different types of ELISA and their applications. 6. What do you mean by DNA sequencing? Why the Sanger’s DNA sequencing method is well flourished in genome sciences? 7. Write a detected account on different types of sequence alignment programmes. Which you have studied in your syllabus? 1 DE–9064 8. What is phylogeny? What are the methods are adapted to analyze the phylogenetic relationship among flora and fauna through bioinformatics? Elaborate your account. ———————— DE–977 12 DISTANCE EDUCATION M. Phil. (Zoology) (NS) DEGREE EXAMINATION, DECEMBER 2010. ANIMAL DIVERSITY, RESOURCES AND CONSERVATION (2006 Batch) Time : Three hours Maximum : 100 marks Answer any FIVE questions. All questions carry equal marks. 1. Explain the recent trends and tools followed in taxonomy. 2. Discuss the role of IUCN on conservation of endangered species. 3. What is genetic diversity? Explain the role of VNTR markers in resolving genetic diversity. 4. Discuss the advantages and disadvantages of Randomly Amplified Polymorphic DNA (RAPD) markers in Phylogenetic comparison. How phylogenetic trees are constructed from RAPD data? 5. Explain the causes of polyarometic hydro carbons (PAH) in marine environment. 6. What are the reasons for ozone depletion? How ozone depletion affects biodiversity? 7. Explain the role of NGOs in minimizing the environmental crisis. 8. Explain the Biogas production with suitable illustrations. ———————— DE–978 13 DISTANCE EDUCATION 2 DE–9064 M.Phil. (Zoology) (Non Semester) DEGREE EXAMINATION, DECEMBER 2010. ANIMAL BIOTECHNOLOGY (2006 Batch) Time : Three hours Maximum : 100 marks Answer any FIVE questions. All questions carry equal marks. 1. Explain the role of various chemical constituents in biological growth media. 2. Describe various methods used to measure apoptosis. 3. What is cell cloning? Explain it in detail. 4. Write a short account on the following : 5. (a) Adenovirus (b) Herpes virus (c) Tissue engineering (d) Apoptosis (e) Cell culture. Explain in detail on intracervical insemination, intrauterine, insemination, intrauterine tuboperitoneal insemination. 6. Write notes on Chemical and Biological methods of gene transfer. 7. Elaborate the application of polymerase chain reaction in diagnosis of genetic disorders. 8. Write an essay on ‘‘Biotechnological approach to pest management and fertility Management’’. ––––––––––– 3 DE–9064